Click here to switch to the map view.

The map label for this gene is purA

Identifier: 170752006

GI number: 170752006

Start: 5967324

End: 5968616

Strand: Direct

Name: purA

Synonym: Mrad2831_5638

Alternate gene names: 170752006

Gene position: 5967324-5968616 (Clockwise)

Preceding gene: 170752005

Following gene: 170752007

Centisome position: 98.18

GC content: 69.61

Gene sequence:

>1293_bases
ATGGCCAACGTCGTCGTTGTGGGCGCCCAGTGGGGCGACGAGGGCAAGGGCAAGATCGTCGACTGGCTCTCCGAGCAGGC
CGACATCGTGGTCCGTTTCCAGGGCGGCCATAATGCCGGCCACACGCTCGTGATCGACGGCGTGACCTACAAGCTGGCGC
TGCTGCCGTCGGGCGTGGTCCGCGGCGGCAAGCTCTCGGTCATCGGCAACGGCGTCGTGGTCGATCCCTGGCACCTGATC
GATGAGATCGAGCGGATCAAGGCGCAGGGCGTGGACGTCTCGCCGTCCTCCTTGCGGATCGCCGACAACGCGACGCTGAT
CCTGCCGCTCCACCGCGAGCTGGATCATTTCCGCGAGACCGCCAATGCCGGGCTGAAGATCGGCACGACTAAGCGCGGCA
TCGGACCGGCCTACGAGGACAAGGTCGGCCGCCGCGCGATCCGGGTCGTCGACCTCGCCGACCCCGACGCGCTGCCCGCC
AAGATCGAGCGCGTGCTGACCCACCACAACGCCCTCCGGCGCGGCCTCGGGATCGACGAGGTGAATGCCGAGGCGCTGCT
GTCCGAGCTGAAGGCGATCGCGCCGAAGATCCTGCCCTTCGCCGACACCGTCTGGTCGCTGCTCGACGAGGAGCGCCGGG
CCGGCAAGCGCATCCTGTTCGAGGGCGCGCAGGGCGCGCTGCTCGACGTCGATCACGGCACCTACCCGTTCGTGACCTCC
TCCAACATCGTGGCCGCGCAGGCGGCGACGGGCTCGGGCCTCGGCCCGGGGGCGATCGGCTACGTCCTGGGCATCGCCAA
GGCGTACACGACCCGCGTCGGCGAGGGGCCGTTCCCGACCGAGCTGTTCGACGAGATCGGCGAGACCATCGGCAGCCGCG
GGCGGGAGTTCGGGGTCAATACCGGGCGCAAGCGCCGCTGCGGCTGGTTCGACGCCGTGCTGGTGCGCCAGACGGTCCGG
ACCTCCGGCATCCACGGCATCGCGCTGACCAAGCTCGATATCCTCGACGGGTTCGAGGAGATCCGCGTCTGCACCGGCTA
CATGCTGGACGGCCAGCCGGTCGACCATCTCCCGGCGAGCCAGGCCGCGCAGGCCCGGATCGAGCCGGTCTACGAAGTGA
TCCCGGGCTGGTCGGAGACGACCGCGGGCGCCCGGTCCTGGGCTGATCTGCCCGCGCAGGCGATCAAGTATGTCCGCCGG
ATCGAGGAGCTGATCGGGGCGCCGGTGGCTCTGCTGTCGACCTCCCCCGAGCGCGACGACACGATCCTCATGCACAACCC
GTTCGAGGATTGA

Upstream 100 bases:

>100_bases
TTGATTTCGCTGCCCGGATCGCGGAAACGGCTCACGACGTACGCCGCCTGCACCCGTGGGCGGCGCCGTCCCGGTTTCCG
TCGCGGGTAAGCAGGCAGGC

Downstream 100 bases:

>100_bases
CCCTCCGCGCGCTATCTCGTTGACGACGCGTGTTTTCGGCGGCCCTGCCTGTATCCAGTCCGCGCGGGCTTGATCTAGAA
CGGGCTGGCGGCCGGGCGGT

Product: adenylosuccinate synthase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MANVVVVGAQWGDEGKGKIVDWLSEQADIVVRFQGGHNAGHTLVIDGVTYKLALLPSGVVRGGKLSVIGNGVVVDPWHLI
DEIERIKAQGVDVSPSSLRIADNATLILPLHRELDHFRETANAGLKIGTTKRGIGPAYEDKVGRRAIRVVDLADPDALPA
KIERVLTHHNALRRGLGIDEVNAEALLSELKAIAPKILPFADTVWSLLDEERRAGKRILFEGAQGALLDVDHGTYPFVTS
SNIVAAQAATGSGLGPGAIGYVLGIAKAYTTRVGEGPFPTELFDEIGETIGSRGREFGVNTGRKRRCGWFDAVLVRQTVR
TSGIHGIALTKLDILDGFEEIRVCTGYMLDGQPVDHLPASQAAQARIEPVYEVIPGWSETTAGARSWADLPAQAIKYVRR
IEELIGAPVALLSTSPERDDTILMHNPFED

Sequences:

>Translated_430_residues
MANVVVVGAQWGDEGKGKIVDWLSEQADIVVRFQGGHNAGHTLVIDGVTYKLALLPSGVVRGGKLSVIGNGVVVDPWHLI
DEIERIKAQGVDVSPSSLRIADNATLILPLHRELDHFRETANAGLKIGTTKRGIGPAYEDKVGRRAIRVVDLADPDALPA
KIERVLTHHNALRRGLGIDEVNAEALLSELKAIAPKILPFADTVWSLLDEERRAGKRILFEGAQGALLDVDHGTYPFVTS
SNIVAAQAATGSGLGPGAIGYVLGIAKAYTTRVGEGPFPTELFDEIGETIGSRGREFGVNTGRKRRCGWFDAVLVRQTVR
TSGIHGIALTKLDILDGFEEIRVCTGYMLDGQPVDHLPASQAAQARIEPVYEVIPGWSETTAGARSWADLPAQAIKYVRR
IEELIGAPVALLSTSPERDDTILMHNPFED
>Mature_429_residues
ANVVVVGAQWGDEGKGKIVDWLSEQADIVVRFQGGHNAGHTLVIDGVTYKLALLPSGVVRGGKLSVIGNGVVVDPWHLID
EIERIKAQGVDVSPSSLRIADNATLILPLHRELDHFRETANAGLKIGTTKRGIGPAYEDKVGRRAIRVVDLADPDALPAK
IERVLTHHNALRRGLGIDEVNAEALLSELKAIAPKILPFADTVWSLLDEERRAGKRILFEGAQGALLDVDHGTYPFVTSS
NIVAAQAATGSGLGPGAIGYVLGIAKAYTTRVGEGPFPTELFDEIGETIGSRGREFGVNTGRKRRCGWFDAVLVRQTVRT
SGIHGIALTKLDILDGFEEIRVCTGYMLDGQPVDHLPASQAAQARIEPVYEVIPGWSETTAGARSWADLPAQAIKYVRRI
EELIGAPVALLSTSPERDDTILMHNPFED

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=44.3661971830986, Blast_Score=356, Evalue=3e-98,
Organism=Homo sapiens, GI34577063, Length=427, Percent_Identity=44.7306791569087, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=42.4242424242424, Blast_Score=313, Evalue=3e-85,
Organism=Escherichia coli, GI1790620, Length=429, Percent_Identity=50.3496503496504, Blast_Score=432, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI25146553, Length=426, Percent_Identity=44.6009389671362, Blast_Score=342, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI25146555, Length=426, Percent_Identity=44.6009389671362, Blast_Score=341, Evalue=4e-94,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=41.2587412587413, Blast_Score=329, Evalue=5e-91,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=44.9172576832151, Blast_Score=352, Evalue=3e-97,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_METRJ (B1M1K0)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001758266.1
- ProteinModelPortal:   B1M1K0
- SMR:   B1M1K0
- GeneID:   6141715
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_5638
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   CLSK2613042
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46349; Mature: 46218

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 130-130 BINDING 144-144 BINDING 224-224 BINDING 239-239 BINDING 303-303 BINDING 305-305

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVVGAQWGDEGKGKIVDWLSEQADIVVRFQGGHNAGHTLVIDGVTYKLALLPSGVV
CCCEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEEECCEEEEEECCCCCC
RGGKLSVIGNGVVVDPWHLIDEIERIKAQGVDVSPSSLRIADNATLILPLHRELDHFRET
CCCEEEEEECCEEECHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECHHHHHHHHHH
ANAGLKIGTTKRGIGPAYEDKVGRRAIRVVDLADPDALPAKIERVLTHHNALRRGLGIDE
HCCCEEECCCCCCCCCHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VNAEALLSELKAIAPKILPFADTVWSLLDEERRAGKRILFEGAQGALLDVDHGTYPFVTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNIVAAQAATGSGLGPGAIGYVLGIAKAYTTRVGEGPFPTELFDEIGETIGSRGREFGVN
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCC
TGRKRRCGWFDAVLVRQTVRTSGIHGIALTKLDILDGFEEIRVCTGYMLDGQPVDHLPAS
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHHCEEECCCCCCCCCCH
QAAQARIEPVYEVIPGWSETTAGARSWADLPAQAIKYVRRIEELIGAPVALLSTSPERDD
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
TILMHNPFED
EEEECCCCCC
>Mature Secondary Structure 
ANVVVVGAQWGDEGKGKIVDWLSEQADIVVRFQGGHNAGHTLVIDGVTYKLALLPSGVV
CCEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEEECCEEEEEECCCCCC
RGGKLSVIGNGVVVDPWHLIDEIERIKAQGVDVSPSSLRIADNATLILPLHRELDHFRET
CCCEEEEEECCEEECHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECHHHHHHHHHH
ANAGLKIGTTKRGIGPAYEDKVGRRAIRVVDLADPDALPAKIERVLTHHNALRRGLGIDE
HCCCEEECCCCCCCCCHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VNAEALLSELKAIAPKILPFADTVWSLLDEERRAGKRILFEGAQGALLDVDHGTYPFVTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNIVAAQAATGSGLGPGAIGYVLGIAKAYTTRVGEGPFPTELFDEIGETIGSRGREFGVN
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCC
TGRKRRCGWFDAVLVRQTVRTSGIHGIALTKLDILDGFEEIRVCTGYMLDGQPVDHLPAS
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHHCEEECCCCCCCCCCH
QAAQARIEPVYEVIPGWSETTAGARSWADLPAQAIKYVRRIEELIGAPVALLSTSPERDD
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
TILMHNPFED
EEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA