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The map label for this gene is algI [H]

Identifier: 170751922

GI number: 170751922

Start: 5873510

End: 5874883

Strand: Direct

Name: algI [H]

Synonym: Mrad2831_5554

Alternate gene names: 170751922

Gene position: 5873510-5874883 (Clockwise)

Preceding gene: 170751921

Following gene: 170751923

Centisome position: 96.64

GC content: 70.45

Gene sequence:

>1374_bases
ATGCTGTTCAACTCCTTCGTCTTCCTCCTCGCCTTCCTGCCGACCGCGCTGATCCTGCACGGGCTGGTGGCGCGCGCCGC
GCCCGCGTGGCGGTTGCCGCTCCTCGTCGCCCTGTCCTTCGTGTTCTACGGCTGGTGGGACATCCGGTTCGTGCCGCTGC
TGGCCGGGTCGATCCTCGCGAACTGGTGCGTGGCCCGGGCCTATCGCCTCTGGCCGGGCCGCTGGCTGATCCCGGCGGCG
ATCGCCGCCAACCTCGCGCTGCTCGGCGTGTTCAAGTACCTCGACTTCGCGGCCGACCTCGCCAACCTGATCCCCGGCCT
GGAAGCGCAGCGCCTCGGCCTCGCCCTGCCGCTCGGCATCTCGTTCTTCACCTTCCACCACATCATGTATCTCGCCGACC
TGCGGGCCGGCCGGGCGCCGGGCTTCGGGCTCGTGAAATACGCCCTCTACATCGCCTTCTTCCCGCAGGTTCTCGCCGGC
CCGCTCGTGCGCTGGAGCGAGATCATGCACCAGTTCGACGAGTCGCCCTACGCGCGGCCCGACGCCGCCGAGCGCTTCGC
CCGCGGGCTGATGCTGCTCACCGTCGGCCTCGCCAAGAAGGTGTTCCTCGGCGACCCGCTCGCCGAGTACGTGAACCCGG
TGTTCCAGGCCGCCGCCGCCGGCAAGGCCGTTACGGTGGTGGAGGCGTGGCAGGCGACGCTCGGCTTCACCTTCCAGATC
TACTTCGACTTCTCCGGCTACACCGACATGGCGCTGGGCATCGCGCTGCTGTTCGGCCTCGTCCTGCCGCAGAACTTCGA
CGTGCCGTACCGCGCCACCTCCCTGCGCGACTTCTGGCGGCGCTGGCACATGACCCTGTCGCGCTTCCTGCGCGACTACC
TGTACGTCCCGATGGGCGGTAACCGCCGCGGGCTGCCGCGGCAGGTCGGGGCGCTCGTCGCCACGATGACGCTCGGCGGC
CTCTGGCACGGGGCGGGCCTGACCTTCCTCGCCTGGGGCGCGCTCCACGGGATCGGCCTCGGCGCCGGGGTCCTGGCGCG
CCGTGCCCGGATCGCGGTGCCCGCGCCCCTCGGCTGGGCGCTGACCAGCCTGTTCGTCGTGCTGACCTGGGTGCTGTTCC
GGGCGACGAGCTTCGAGGCGGCCCTCGTGATCTTCAAGGGGCTGTTCGGGCTGGCGCCCAGGGGCTCCGGCTTCAAGTGG
CGCACCATCGCCATCGCCGCCCTGGTCGCGACGGTGGGGCCCACCGCGTGGAACGCGGTGCATCGCCTGCCGCCCCGGCG
GCTCCTCGCCTTCGGCTTCGCGCTGCTGTTCGTGCTCGTCCTGCTCAAGATCGGCGACGATGCGAACTACGAGTTCATCT
ACTTCCAGTTCTGA

Upstream 100 bases:

>100_bases
GAGCGCCGATTGACGGGCCGGCCCCGATCCCCGACAAGGCCGTCCCTCCCGCTCGGAGACTACGGCGGCCCATCCGGGCT
CCTCCGCGCCGCCCCGCCCG

Downstream 100 bases:

>100_bases
GGATGGCGCCCGCACCCCCCACGACCGATCGAGACTGGCGCGGCTTCGCCCGCACCCTGTTCCTGTCGATGGTCGCCCTG
TTCGCGGGCTACGTGGCGCT

Product: membrane bound O-acyl transferase MBOAT family protein

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MLFNSFVFLLAFLPTALILHGLVARAAPAWRLPLLVALSFVFYGWWDIRFVPLLAGSILANWCVARAYRLWPGRWLIPAA
IAANLALLGVFKYLDFAADLANLIPGLEAQRLGLALPLGISFFTFHHIMYLADLRAGRAPGFGLVKYALYIAFFPQVLAG
PLVRWSEIMHQFDESPYARPDAAERFARGLMLLTVGLAKKVFLGDPLAEYVNPVFQAAAAGKAVTVVEAWQATLGFTFQI
YFDFSGYTDMALGIALLFGLVLPQNFDVPYRATSLRDFWRRWHMTLSRFLRDYLYVPMGGNRRGLPRQVGALVATMTLGG
LWHGAGLTFLAWGALHGIGLGAGVLARRARIAVPAPLGWALTSLFVVLTWVLFRATSFEAALVIFKGLFGLAPRGSGFKW
RTIAIAALVATVGPTAWNAVHRLPPRRLLAFGFALLFVLVLLKIGDDANYEFIYFQF

Sequences:

>Translated_457_residues
MLFNSFVFLLAFLPTALILHGLVARAAPAWRLPLLVALSFVFYGWWDIRFVPLLAGSILANWCVARAYRLWPGRWLIPAA
IAANLALLGVFKYLDFAADLANLIPGLEAQRLGLALPLGISFFTFHHIMYLADLRAGRAPGFGLVKYALYIAFFPQVLAG
PLVRWSEIMHQFDESPYARPDAAERFARGLMLLTVGLAKKVFLGDPLAEYVNPVFQAAAAGKAVTVVEAWQATLGFTFQI
YFDFSGYTDMALGIALLFGLVLPQNFDVPYRATSLRDFWRRWHMTLSRFLRDYLYVPMGGNRRGLPRQVGALVATMTLGG
LWHGAGLTFLAWGALHGIGLGAGVLARRARIAVPAPLGWALTSLFVVLTWVLFRATSFEAALVIFKGLFGLAPRGSGFKW
RTIAIAALVATVGPTAWNAVHRLPPRRLLAFGFALLFVLVLLKIGDDANYEFIYFQF
>Mature_457_residues
MLFNSFVFLLAFLPTALILHGLVARAAPAWRLPLLVALSFVFYGWWDIRFVPLLAGSILANWCVARAYRLWPGRWLIPAA
IAANLALLGVFKYLDFAADLANLIPGLEAQRLGLALPLGISFFTFHHIMYLADLRAGRAPGFGLVKYALYIAFFPQVLAG
PLVRWSEIMHQFDESPYARPDAAERFARGLMLLTVGLAKKVFLGDPLAEYVNPVFQAAAAGKAVTVVEAWQATLGFTFQI
YFDFSGYTDMALGIALLFGLVLPQNFDVPYRATSLRDFWRRWHMTLSRFLRDYLYVPMGGNRRGLPRQVGALVATMTLGG
LWHGAGLTFLAWGALHGIGLGAGVLARRARIAVPAPLGWALTSLFVVLTWVLFRATSFEAALVIFKGLFGLAPRGSGFKW
RTIAIAALVATVGPTAWNAVHRLPPRRLLAFGFALLFVLVLLKIGDDANYEFIYFQF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 50576; Mature: 50576

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFNSFVFLLAFLPTALILHGLVARAAPAWRLPLLVALSFVFYGWWDIRFVPLLAGSILA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NWCVARAYRLWPGRWLIPAAIAANLALLGVFKYLDFAADLANLIPGLEAQRLGLALPLGI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEHHHHH
SFFTFHHIMYLADLRAGRAPGFGLVKYALYIAFFPQVLAGPLVRWSEIMHQFDESPYARP
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DAAERFARGLMLLTVGLAKKVFLGDPLAEYVNPVFQAAAAGKAVTVVEAWQATLGFTFQI
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCEEEEE
YFDFSGYTDMALGIALLFGLVLPQNFDVPYRATSLRDFWRRWHMTLSRFLRDYLYVPMGG
EEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCC
NRRGLPRQVGALVATMTLGGLWHGAGLTFLAWGALHGIGLGAGVLARRARIAVPAPLGWA
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHEECCCCHHHH
LTSLFVVLTWVLFRATSFEAALVIFKGLFGLAPRGSGFKWRTIAIAALVATVGPTAWNAV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
HRLPPRRLLAFGFALLFVLVLLKIGDDANYEFIYFQF
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
>Mature Secondary Structure
MLFNSFVFLLAFLPTALILHGLVARAAPAWRLPLLVALSFVFYGWWDIRFVPLLAGSILA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NWCVARAYRLWPGRWLIPAAIAANLALLGVFKYLDFAADLANLIPGLEAQRLGLALPLGI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCEEHHHHH
SFFTFHHIMYLADLRAGRAPGFGLVKYALYIAFFPQVLAGPLVRWSEIMHQFDESPYARP
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DAAERFARGLMLLTVGLAKKVFLGDPLAEYVNPVFQAAAAGKAVTVVEAWQATLGFTFQI
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCEEEEE
YFDFSGYTDMALGIALLFGLVLPQNFDVPYRATSLRDFWRRWHMTLSRFLRDYLYVPMGG
EEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCC
NRRGLPRQVGALVATMTLGGLWHGAGLTFLAWGALHGIGLGAGVLARRARIAVPAPLGWA
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHEECCCCHHHH
LTSLFVVLTWVLFRATSFEAALVIFKGLFGLAPRGSGFKWRTIAIAALVATVGPTAWNAV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
HRLPPRRLLAFGFALLFVLVLLKIGDDANYEFIYFQF
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8636017; 10984043; 12003941; 11208804; 11179370 [H]