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The map label for this gene is otsB [H]

Identifier: 170751861

GI number: 170751861

Start: 5807066

End: 5807809

Strand: Direct

Name: otsB [H]

Synonym: Mrad2831_5493

Alternate gene names: 170751861

Gene position: 5807066-5807809 (Clockwise)

Preceding gene: 170751858

Following gene: 170751862

Centisome position: 95.55

GC content: 75.0

Gene sequence:

>744_bases
ATGGACATCGCCCTCTTCCTCGACTTCGACGGCACGCTCGCCGAGATCGCTCCGCGGCCGGATGCCGTGCGGGTCGAGCC
GGGTCTGGTGGAGGATCTGGAGCGTCTCCGGGCACGCCTCGACGGCGCCCTCGCCATCGTCACCGGCCGGCCAGTCACGG
TGATCGACGGCTTCCTGACGCCCGCGCGCCTCGACGCGGCCGGTCTTCACGGCGTGGAGCGCCGGGTCGACGGCGCGCTC
ACCGGCGGCCGCGCGGAGGATCACCCCGACCTGCGCCGGCAGGTCGCGCGCCTGCAGGCCGAGACCGCCGCGCTGGCGCA
GGTCCTGATCGAGGACAAGGGCGCCTCAGTGGCGGTCCACTGGCGGCTCGCGAGCCCGGACGACGCGCTGCGGGCCGAGA
CGCTGGTGAAGGGCGCGGCGGAGGCCCTCGGCGCCGATTATCGCCTCCAGCTCGGCAAGGCGGTCGGCGAGATCGTCCCC
GCGCAGGCCACCAAAGCCCACGCGATCCGGGCCTTCATGGCGCAGGCGCCCTACGCGGGACGGATTCCCGTCTTCTTCGG
CGACGACCGCACCGACGAGATCGCCTTCGCGTCGGTGAACGAGGACGGCGGCGTCGCGGTGCGCGTCGGGGACGGCGAGA
CCGTCGCCCGGCGACGCCTGCCCGACCCCGCCGCCGTCCGTGCCCTGCTGAGCAACTGGGCCGCCGGCGGTGCGATCGAC
CCGGACGCGCTGCCGCCGGCCTGA

Upstream 100 bases:

>100_bases
TGTGTCAGTGCGGATCCAGATAGGGCGCCGCGGCCGGCGTTTGTCGTCCGGCGCGCCCGTGCTAGCCCGTCGCCGACACT
CGAGCCCGGGATCGCCCGCG

Downstream 100 bases:

>100_bases
CGCGCCGCGCCGTCCAGGGGAGATCGCGATGACGGGGCGTTCCGTCACGGGCTGGCCGCGCCTGCTGCTGCGGCTGGAGG
GAGCCTGCATCCTGGTCGCC

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MDIALFLDFDGTLAEIAPRPDAVRVEPGLVEDLERLRARLDGALAIVTGRPVTVIDGFLTPARLDAAGLHGVERRVDGAL
TGGRAEDHPDLRRQVARLQAETAALAQVLIEDKGASVAVHWRLASPDDALRAETLVKGAAEALGADYRLQLGKAVGEIVP
AQATKAHAIRAFMAQAPYAGRIPVFFGDDRTDEIAFASVNEDGGVAVRVGDGETVARRRLPDPAAVRALLSNWAAGGAID
PDALPPA

Sequences:

>Translated_247_residues
MDIALFLDFDGTLAEIAPRPDAVRVEPGLVEDLERLRARLDGALAIVTGRPVTVIDGFLTPARLDAAGLHGVERRVDGAL
TGGRAEDHPDLRRQVARLQAETAALAQVLIEDKGASVAVHWRLASPDDALRAETLVKGAAEALGADYRLQLGKAVGEIVP
AQATKAHAIRAFMAQAPYAGRIPVFFGDDRTDEIAFASVNEDGGVAVRVGDGETVARRRLPDPAAVRALLSNWAAGGAID
PDALPPA
>Mature_247_residues
MDIALFLDFDGTLAEIAPRPDAVRVEPGLVEDLERLRARLDGALAIVTGRPVTVIDGFLTPARLDAAGLHGVERRVDGAL
TGGRAEDHPDLRRQVARLQAETAALAQVLIEDKGASVAVHWRLASPDDALRAETLVKGAAEALGADYRLQLGKAVGEIVP
AQATKAHAIRAFMAQAPYAGRIPVFFGDDRTDEIAFASVNEDGGVAVRVGDGETVARRRLPDPAAVRALLSNWAAGGAID
PDALPPA

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=221, Percent_Identity=42.0814479638009, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 25995; Mature: 25995

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIALFLDFDGTLAEIAPRPDAVRVEPGLVEDLERLRARLDGALAIVTGRPVTVIDGFLT
CCEEEEEECCCCHHHHCCCCCCEEECCCHHHHHHHHHHHHCCEEEEEECCCEEEEECCCC
PARLDAAGLHGVERRVDGALTGGRAEDHPDLRRQVARLQAETAALAQVLIEDKGASVAVH
CHHHCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
WRLASPDDALRAETLVKGAAEALGADYRLQLGKAVGEIVPAQATKAHAIRAFMAQAPYAG
EEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
RIPVFFGDDRTDEIAFASVNEDGGVAVRVGDGETVARRRLPDPAAVRALLSNWAAGGAID
CCCEEECCCCCCCEEEEEECCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHCCCCCCCC
PDALPPA
CCCCCCC
>Mature Secondary Structure
MDIALFLDFDGTLAEIAPRPDAVRVEPGLVEDLERLRARLDGALAIVTGRPVTVIDGFLT
CCEEEEEECCCCHHHHCCCCCCEEECCCHHHHHHHHHHHHCCEEEEEECCCEEEEECCCC
PARLDAAGLHGVERRVDGALTGGRAEDHPDLRRQVARLQAETAALAQVLIEDKGASVAVH
CHHHCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
WRLASPDDALRAETLVKGAAEALGADYRLQLGKAVGEIVPAQATKAHAIRAFMAQAPYAG
EEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
RIPVFFGDDRTDEIAFASVNEDGGVAVRVGDGETVARRRLPDPAAVRALLSNWAAGGAID
CCCEEECCCCCCCEEEEEECCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHCCCCCCCC
PDALPPA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]