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The map label for this gene is yfcG [H]

Identifier: 170751810

GI number: 170751810

Start: 5747704

End: 5748411

Strand: Direct

Name: yfcG [H]

Synonym: Mrad2831_5440

Alternate gene names: 170751810

Gene position: 5747704-5748411 (Clockwise)

Preceding gene: 170751809

Following gene: 170751811

Centisome position: 94.57

GC content: 64.83

Gene sequence:

>708_bases
ATGATCGAACTCCACTACTGGCCGACGCCGAACGGCCATAAGATCACGATGTTTCTCGAAGAGGCGGGCATTCCCTACCG
TATTCACCCAGTCAATATTGGTAAGGGCGAGCAGTTCAATCCCGATTTCCTCAAGATCGCGCCCAACAACCGGATGCCGG
CGATCGTCGACGACGAGCCGGCGGGCGGCGGCGCGCCGGTCTCGCTGTTCGAGTCGGGGGCGATCCTGCTCTACCTCGCC
GAGAAGACCGGGCGCTTCATCCCGGGCGACCTGCGGGGCCGGGCCGAGGTGCTGCAGTGGTTGTTCTGGCAGATGGGCGG
CCTCGGCCCGATGCTCGGCCAGAACCATCACTTCTCGCAGTACGCGCCGGAGAAGATCGACTACGCGATCAAGCGCTACG
TCAACGAGACCAACCGGCTCTACGGCGTCCTCGACCGGCGGCTGGCCGACCGCGCCTTCGTCGCCGGGCCGGACTACTCC
ATCGCCGACATGGCCTGCTATCCCTGGATCGTGCCATGGGAGAAGCAGGGTCAGAACCTCGACGAGCACCCGAACCTCAA
GCGCTGGTTCACGGCGATCGCCGAGCGGCCCGCCACCAAGGCGGCTTATGCCCGCGCCCCGGAGGTCAATCCCGACTTCG
GCAAGACCATGAGCGAGGATGCCAAGAAGGTGATGTTCGGACAGACCGCCTCCAGCACCAGGCGCTGA

Upstream 100 bases:

>100_bases
CCCGATACCGGGGCCCGAGCCCGCCGCCGGGCTCATGCTTGACCGGAGCGCCGCGCCGACCCACTTCCCGTCCGCCGGGG
GCATAGCGGGACACCGACCG

Downstream 100 bases:

>100_bases
CCGGACCATGACCGAGACCCTGCCCGCGCTGCCGCTGGAGGTGATCCACCGCGGCTGGAACACCTTCGGCATCGCCACCC
TGACGCTGCCGGATGGCAGC

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MIELHYWPTPNGHKITMFLEEAGIPYRIHPVNIGKGEQFNPDFLKIAPNNRMPAIVDDEPAGGGAPVSLFESGAILLYLA
EKTGRFIPGDLRGRAEVLQWLFWQMGGLGPMLGQNHHFSQYAPEKIDYAIKRYVNETNRLYGVLDRRLADRAFVAGPDYS
IADMACYPWIVPWEKQGQNLDEHPNLKRWFTAIAERPATKAAYARAPEVNPDFGKTMSEDAKKVMFGQTASSTRR

Sequences:

>Translated_235_residues
MIELHYWPTPNGHKITMFLEEAGIPYRIHPVNIGKGEQFNPDFLKIAPNNRMPAIVDDEPAGGGAPVSLFESGAILLYLA
EKTGRFIPGDLRGRAEVLQWLFWQMGGLGPMLGQNHHFSQYAPEKIDYAIKRYVNETNRLYGVLDRRLADRAFVAGPDYS
IADMACYPWIVPWEKQGQNLDEHPNLKRWFTAIAERPATKAAYARAPEVNPDFGKTMSEDAKKVMFGQTASSTRR
>Mature_235_residues
MIELHYWPTPNGHKITMFLEEAGIPYRIHPVNIGKGEQFNPDFLKIAPNNRMPAIVDDEPAGGGAPVSLFESGAILLYLA
EKTGRFIPGDLRGRAEVLQWLFWQMGGLGPMLGQNHHFSQYAPEKIDYAIKRYVNETNRLYGVLDRRLADRAFVAGPDYS
IADMACYPWIVPWEKQGQNLDEHPNLKRWFTAIAERPATKAAYARAPEVNPDFGKTMSEDAKKVMFGQTASSTRR

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=206, Percent_Identity=59.7087378640777, Blast_Score=260, Evalue=5e-71,
Organism=Escherichia coli, GI87082195, Length=213, Percent_Identity=42.2535211267606, Blast_Score=161, Evalue=3e-41,
Organism=Escherichia coli, GI87081789, Length=201, Percent_Identity=30.8457711442786, Blast_Score=73, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324100, Length=232, Percent_Identity=33.1896551724138, Blast_Score=114, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28571670, Length=179, Percent_Identity=31.2849162011173, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17864598, Length=201, Percent_Identity=29.3532338308458, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI17864592, Length=195, Percent_Identity=27.6923076923077, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17933730, Length=194, Percent_Identity=27.319587628866, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646249, Length=199, Percent_Identity=28.1407035175879, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17864594, Length=195, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45549270, Length=195, Percent_Identity=26.1538461538462, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI85725204, Length=195, Percent_Identity=25.6410256410256, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17737923, Length=195, Percent_Identity=25.6410256410256, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26552; Mature: 26552

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELHYWPTPNGHKITMFLEEAGIPYRIHPVNIGKGEQFNPDFLKIAPNNRMPAIVDDEP
CEEEEECCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCEEECCCC
AGGGAPVSLFESGAILLYLAEKTGRFIPGDLRGRAEVLQWLFWQMGGLGPMLGQNHHFSQ
CCCCCCHHEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHH
YAPEKIDYAIKRYVNETNRLYGVLDRRLADRAFVAGPDYSIADMACYPWIVPWEKQGQNL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHCCCCCCHHHCCCCC
DEHPNLKRWFTAIAERPATKAAYARAPEVNPDFGKTMSEDAKKVMFGQTASSTRR
CCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MIELHYWPTPNGHKITMFLEEAGIPYRIHPVNIGKGEQFNPDFLKIAPNNRMPAIVDDEP
CEEEEECCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCEEECCCC
AGGGAPVSLFESGAILLYLAEKTGRFIPGDLRGRAEVLQWLFWQMGGLGPMLGQNHHFSQ
CCCCCCHHEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHH
YAPEKIDYAIKRYVNETNRLYGVLDRRLADRAFVAGPDYSIADMACYPWIVPWEKQGQNL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHCCCCCCHHHCCCCC
DEHPNLKRWFTAIAERPATKAAYARAPEVNPDFGKTMSEDAKKVMFGQTASSTRR
CCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]