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The map label for this gene is glnA [H]

Identifier: 170751507

GI number: 170751507

Start: 5448582

End: 5449991

Strand: Reverse

Name: glnA [H]

Synonym: Mrad2831_5127

Alternate gene names: 170751507

Gene position: 5449991-5448582 (Counterclockwise)

Preceding gene: 170751509

Following gene: 170751506

Centisome position: 89.67

GC content: 65.74

Gene sequence:

>1410_bases
ATGACCACTGCGGCCGAGGTGCTGAAGACGATCCGGGACAACGACGTGCGTTACGTGGACTTCCGGTTCACCGACCCGCG
CGGCAAGTGGCAGCACGTCACGTTCGACGTGTCGCTCATCGACGAGGAGATCTTCGACGAAGGCACCATGTTCGACGGCT
CCTCGATCGCCGGCTGGAAGGCGATCAACGAGTCCGACATGTTGCTGATGCCGGATCCGGTGACCGCCACGCTGGACCCG
TTCTTCTCGGCCGCGACCATGTCGATCGTCTGCGACGTGCTCGAGCCGGCGACCGGCGAGCCCTACGGCCGCGATCCGCG
CGGCACCGCCAAGCGCGCCGAGGCCTACCTGCAGCAGACCGGCATCGGCGACACGATCTATGTCGGCCCCGAGGCCGAGT
TCTTCGTGTTCGACGACGTCAAGTTCGGCGCCGACCCGTACCGCACCGGCTTCGAGCTCGACTCCACCGAGCTGCCGACC
AACGGCTTCACCGACTATGAGGGCGGCAACCTCGGCCACCGGGTGCAGACCAAGGGCGGCTATTTCCCGGTGCCGCCGCA
GGATTCGGCGCAGGACATGCGCGGCGAGATGCTCGCGGCCATGCAGTCCATGGGCGTGAAGGTCGAGAAGCACCACCACG
AGGTGGCTTCGGCCCAGCACGAGCTCGGCATGAAGTTCGACACGCTGACCCTGCTCGCCGACCACATGCAGATCTACAAG
TACTGCATCCACAACGTCGCGCAGAGCTACGGCAAGTCGGCGACGTTCATGCCGAAGCCCGTCTACGGCGACAACGGCTC
GGGCATGCACGTCCACCAGTCGATCTGGAAGGACGGCAAGCCGGTCTTCGCCGGCGACAAGTATGCCGGCCTGTCCCAGG
AGTGCCTCTGGTACATCGGCGGCATCATCAAGCACGCCAAGGCGCTCAACGCCTTCACCAATCCGTCGACCAACTCGTAC
AAGCGGCTCGTGCCGGGCTACGAGGCGCCGGTGCTGCTCGCCTACTCGGCGCGCAACCGCTCGGCCTCCTGCCGCATCCC
GTGGACCAAGAGCCCGAAGGCCAAGCGCGTCGAGGTCCGCTTCCCCGACCCGATGGCCAACCCCTACCTCGCCTTCTCGG
CGCTGCTGATGGCCGGCCTCGACGGTATCCGGAACAAGATCGACCCGGGCCCGGCCATGGACAAGGACCTGTACGAGCTG
CCGCCGCGGGAGCTCAAGAAGATCCCGACCGTGTGCGGCTCGCTGCGCGAGGCCCTGAACAGCCTCGACAAGGACCGGGC
CTTCCTCAAGGAGGGCGGCGTGTTCTCGGACGACCAGATCGACTCGTTCATCGAGCTGAAGATGACCGAGGTGCTGCGCT
ACGAGATGACCCCGCACCCGATCGAGTTCGTGCAGTACTACTCGCTGTGA

Upstream 100 bases:

>100_bases
GCACGCCCGCGCCGGATATCAAGCGCCGCCGGCCGGCGCGCGCGACCGCGTTCGGGTGCGCCACGAGAGACGGTTCAGAG
ATCCCTGGAGGATGCCCACG

Downstream 100 bases:

>100_bases
GGTGACAGGGGCCGGGCGCGACGCCCGGCCCGGCACCCTCTCCCGGGCCGGGGCCTCTCGCGGGGCTCCGGCCTTCTTCT
TGCGGCCGCTCCGGCGTTAT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTTAAEVLKTIRDNDVRYVDFRFTDPRGKWQHVTFDVSLIDEEIFDEGTMFDGSSIAGWKAINESDMLLMPDPVTATLDP
FFSAATMSIVCDVLEPATGEPYGRDPRGTAKRAEAYLQQTGIGDTIYVGPEAEFFVFDDVKFGADPYRTGFELDSTELPT
NGFTDYEGGNLGHRVQTKGGYFPVPPQDSAQDMRGEMLAAMQSMGVKVEKHHHEVASAQHELGMKFDTLTLLADHMQIYK
YCIHNVAQSYGKSATFMPKPVYGDNGSGMHVHQSIWKDGKPVFAGDKYAGLSQECLWYIGGIIKHAKALNAFTNPSTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPWTKSPKAKRVEVRFPDPMANPYLAFSALLMAGLDGIRNKIDPGPAMDKDLYEL
PPRELKKIPTVCGSLREALNSLDKDRAFLKEGGVFSDDQIDSFIELKMTEVLRYEMTPHPIEFVQYYSL

Sequences:

>Translated_469_residues
MTTAAEVLKTIRDNDVRYVDFRFTDPRGKWQHVTFDVSLIDEEIFDEGTMFDGSSIAGWKAINESDMLLMPDPVTATLDP
FFSAATMSIVCDVLEPATGEPYGRDPRGTAKRAEAYLQQTGIGDTIYVGPEAEFFVFDDVKFGADPYRTGFELDSTELPT
NGFTDYEGGNLGHRVQTKGGYFPVPPQDSAQDMRGEMLAAMQSMGVKVEKHHHEVASAQHELGMKFDTLTLLADHMQIYK
YCIHNVAQSYGKSATFMPKPVYGDNGSGMHVHQSIWKDGKPVFAGDKYAGLSQECLWYIGGIIKHAKALNAFTNPSTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPWTKSPKAKRVEVRFPDPMANPYLAFSALLMAGLDGIRNKIDPGPAMDKDLYEL
PPRELKKIPTVCGSLREALNSLDKDRAFLKEGGVFSDDQIDSFIELKMTEVLRYEMTPHPIEFVQYYSL
>Mature_468_residues
TTAAEVLKTIRDNDVRYVDFRFTDPRGKWQHVTFDVSLIDEEIFDEGTMFDGSSIAGWKAINESDMLLMPDPVTATLDPF
FSAATMSIVCDVLEPATGEPYGRDPRGTAKRAEAYLQQTGIGDTIYVGPEAEFFVFDDVKFGADPYRTGFELDSTELPTN
GFTDYEGGNLGHRVQTKGGYFPVPPQDSAQDMRGEMLAAMQSMGVKVEKHHHEVASAQHELGMKFDTLTLLADHMQIYKY
CIHNVAQSYGKSATFMPKPVYGDNGSGMHVHQSIWKDGKPVFAGDKYAGLSQECLWYIGGIIKHAKALNAFTNPSTNSYK
RLVPGYEAPVLLAYSARNRSASCRIPWTKSPKAKRVEVRFPDPMANPYLAFSALLMAGLDGIRNKIDPGPAMDKDLYELP
PRELKKIPTVCGSLREALNSLDKDRAFLKEGGVFSDDQIDSFIELKMTEVLRYEMTPHPIEFVQYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=482, Percent_Identity=22.6141078838174, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=63.2478632478632, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=387, Percent_Identity=24.8062015503876, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52352; Mature: 52221

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAAEVLKTIRDNDVRYVDFRFTDPRGKWQHVTFDVSLIDEEIFDEGTMFDGSSIAGWK
CCCHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCE
AINESDMLLMPDPVTATLDPFFSAATMSIVCDVLEPATGEPYGRDPRGTAKRAEAYLQQT
ECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHC
GIGDTIYVGPEAEFFVFDDVKFGADPYRTGFELDSTELPTNGFTDYEGGNLGHRVQTKGG
CCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCC
YFPVPPQDSAQDMRGEMLAAMQSMGVKVEKHHHEVASAQHELGMKFDTLTLLADHMQIYK
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YCIHNVAQSYGKSATFMPKPVYGDNGSGMHVHQSIWKDGKPVFAGDKYAGLSQECLWYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEECCCCCCCCCHHHHHHHH
GIIKHAKALNAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPWTKSPKAKRVEVR
HHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEE
FPDPMANPYLAFSALLMAGLDGIRNKIDPGPAMDKDLYELPPRELKKIPTVCGSLREALN
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
SLDKDRAFLKEGGVFSDDQIDSFIELKMTEVLRYEMTPHPIEFVQYYSL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure 
TTAAEVLKTIRDNDVRYVDFRFTDPRGKWQHVTFDVSLIDEEIFDEGTMFDGSSIAGWK
CCHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCE
AINESDMLLMPDPVTATLDPFFSAATMSIVCDVLEPATGEPYGRDPRGTAKRAEAYLQQT
ECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHC
GIGDTIYVGPEAEFFVFDDVKFGADPYRTGFELDSTELPTNGFTDYEGGNLGHRVQTKGG
CCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCC
YFPVPPQDSAQDMRGEMLAAMQSMGVKVEKHHHEVASAQHELGMKFDTLTLLADHMQIYK
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YCIHNVAQSYGKSATFMPKPVYGDNGSGMHVHQSIWKDGKPVFAGDKYAGLSQECLWYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEECCCCCCCCCHHHHHHHH
GIIKHAKALNAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPWTKSPKAKRVEVR
HHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEE
FPDPMANPYLAFSALLMAGLDGIRNKIDPGPAMDKDLYELPPRELKKIPTVCGSLREALN
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
SLDKDRAFLKEGGVFSDDQIDSFIELKMTEVLRYEMTPHPIEFVQYYSL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275; 2859270; 2793830 [H]