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The map label for this gene is macA [H]

Identifier: 170751484

GI number: 170751484

Start: 5427707

End: 5428990

Strand: Reverse

Name: macA [H]

Synonym: Mrad2831_5104

Alternate gene names: 170751484

Gene position: 5428990-5427707 (Counterclockwise)

Preceding gene: 170751486

Following gene: 170751483

Centisome position: 89.32

GC content: 75.62

Gene sequence:

>1284_bases
ATGAAAACGCCGACCTGCCCAGCGAGGGAGAGGCAATACCCGTCCAGCGCCGACTGTGAGGTCCCCGCCGTGAACTCCGT
CCGTTGGCGCGCGTCTCGCCTCAGTCTCCTGTGCGGGCTTGCGATGCTCGCGGCGGCGCCGGCCCGGGCGGTCGAGACCG
CCGCCGTTCCCGACGCCGCCGCCATCGCGCCCGCGGTCACGGTCGTGCCGGCCGAGAGCCGCGAGATCGTCGAGCGGGCC
GTCGTCACCGGCACACTGGTCCCCCGGGACGAGATCCTCGTCTCCCCCGAGGTCGAGGGTCTGCGCATCACCGATCTCCT
CGTCGAGGAGGGTGACCGCGTCACCAAGGGACAGGTTCTCGCCAAGCTGTCGCTGGAGATGATCGTCACCCAGGAGGCCT
CGAACGCGGCGGCGATCGCCCGCGCCGAGGCGGCCATCGTCCAGGCCCGGAGCCAGATCGTCCAGGCGGAGGCCGCCAAC
CTGGAGGCCAAGCAGGCGCTCGAGCGGGCCCAGGCGCTCGCCAAGACCGGCAACGCCACCGCGGCCGTGCTGGAACAGCG
CGTCTCGGCGGCGCAGGGGGCGGAAGGCCGCCTCGCCGCCGCCAGGGGCGGCCTGCAATCGGCCCAGGCCGACCTCGCCA
CGGCGCGGGCCGCCGGGGCCGAGATCGCCCTGCGGCGCGCTCGCACCGACATCCGCGCCCCCGAAGCCGGGATCGTCAAC
CGCCGCACCGCCCGGATCGGCGCCACCGCCACGGCGGCGGGCGAGCCGCTGTTCCGGCTGATCGCCCGGGGCGAGATCGA
ATTGGAGGGCGAGGTGCCCGAGACGTCGCTGGCGCGCATCCACGTCGGCAATCCGGCGAGCCTCGACCTCGATGACGGTC
GCCGCCTCGCCGGCAAGGTGCGCCGGGTCTACCCGGAGGTCGACCGGGCGACGCGGCTCGGCAAGGTGCGCATCCGCCTC
GGCGATGATCCGGCCCTGCGCATCGGCGCCTTCGCGCGCGGCAGCGTGGAGATCGCGCGCCGCACCGGCGTCGCCGTCCC
GGTCTCCAGCCTGCTCTACGCGGCCGACGGCCGCGCCAGCGTGCTCGTGGCGAGGGACGGGCGCGTCGAGGCGCGGCCCG
TGACCACGGGCCTCTCCGCCGACGGCTTCACGGAACTCCGCTCCGGCGTCGCGGCCGGCGAGAGCGTCGTGGCCCGCGCC
GGAAGCTTCCTGCGCGACGGCGACCGCGTGCGCCCCGTCCCGGCCGCCACCAGCACGCCGATCGCCGACGCGGCGCCGCG
GTAG

Upstream 100 bases:

>100_bases
AAACCGCCGCGCTCCGGCCGCCACGATGACCTGCGGCGCGAGGCCCGGCGACGGCCTCGCGCGACGGCGCTATGCTCGGC
ACCGTCCAGGATGGCTCGCC

Downstream 100 bases:

>100_bases
GACGAGTCGAAGGATCCGGCCATGCGCCTCAACGTCTCCGCCTGGGCGATCCGCAAACCCCTGCCCTCGGTGGTCCTGTT
CCTGGTCCTGATGATCCTCG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKTPTCPARERQYPSSADCEVPAVNSVRWRASRLSLLCGLAMLAAAPARAVETAAVPDAAAIAPAVTVVPAESREIVERA
VVTGTLVPRDEILVSPEVEGLRITDLLVEEGDRVTKGQVLAKLSLEMIVTQEASNAAAIARAEAAIVQARSQIVQAEAAN
LEAKQALERAQALAKTGNATAAVLEQRVSAAQGAEGRLAAARGGLQSAQADLATARAAGAEIALRRARTDIRAPEAGIVN
RRTARIGATATAAGEPLFRLIARGEIELEGEVPETSLARIHVGNPASLDLDDGRRLAGKVRRVYPEVDRATRLGKVRIRL
GDDPALRIGAFARGSVEIARRTGVAVPVSSLLYAADGRASVLVARDGRVEARPVTTGLSADGFTELRSGVAAGESVVARA
GSFLRDGDRVRPVPAATSTPIADAAPR

Sequences:

>Translated_427_residues
MKTPTCPARERQYPSSADCEVPAVNSVRWRASRLSLLCGLAMLAAAPARAVETAAVPDAAAIAPAVTVVPAESREIVERA
VVTGTLVPRDEILVSPEVEGLRITDLLVEEGDRVTKGQVLAKLSLEMIVTQEASNAAAIARAEAAIVQARSQIVQAEAAN
LEAKQALERAQALAKTGNATAAVLEQRVSAAQGAEGRLAAARGGLQSAQADLATARAAGAEIALRRARTDIRAPEAGIVN
RRTARIGATATAAGEPLFRLIARGEIELEGEVPETSLARIHVGNPASLDLDDGRRLAGKVRRVYPEVDRATRLGKVRIRL
GDDPALRIGAFARGSVEIARRTGVAVPVSSLLYAADGRASVLVARDGRVEARPVTTGLSADGFTELRSGVAAGESVVARA
GSFLRDGDRVRPVPAATSTPIADAAPR
>Mature_427_residues
MKTPTCPARERQYPSSADCEVPAVNSVRWRASRLSLLCGLAMLAAAPARAVETAAVPDAAAIAPAVTVVPAESREIVERA
VVTGTLVPRDEILVSPEVEGLRITDLLVEEGDRVTKGQVLAKLSLEMIVTQEASNAAAIARAEAAIVQARSQIVQAEAAN
LEAKQALERAQALAKTGNATAAVLEQRVSAAQGAEGRLAAARGGLQSAQADLATARAAGAEIALRRARTDIRAPEAGIVN
RRTARIGATATAAGEPLFRLIARGEIELEGEVPETSLARIHVGNPASLDLDDGRRLAGKVRRVYPEVDRATRLGKVRIRL
GDDPALRIGAFARGSVEIARRTGVAVPVSSLLYAADGRASVLVARDGRVEARPVTTGLSADGFTELRSGVAAGESVVARA
GSFLRDGDRVRPVPAATSTPIADAAPR

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=401, Percent_Identity=22.6932668329177, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44446; Mature: 44446

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPTCPARERQYPSSADCEVPAVNSVRWRASRLSLLCGLAMLAAAPARAVETAAVPDAA
CCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHH
AIAPAVTVVPAESREIVERAVVTGTLVPRDEILVSPEVEGLRITDLLVEEGDRVTKGQVL
HHCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEHHHHHCCCCCCHHHHH
AKLSLEMIVTQEASNAAAIARAEAAIVQARSQIVQAEAANLEAKQALERAQALAKTGNAT
HHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHH
AAVLEQRVSAAQGAEGRLAAARGGLQSAQADLATARAAGAEIALRRARTDIRAPEAGIVN
HHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
RRTARIGATATAAGEPLFRLIARGEIELEGEVPETSLARIHVGNPASLDLDDGRRLAGKV
CCHHCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCEEEEECCCCCEECCCCCHHHHHHH
RRVYPEVDRATRLGKVRIRLGDDPALRIGAFARGSVEIARRTGVAVPVSSLLYAADGRAS
HHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCHHHHHCCCCCCHHHHHEECCCCEE
VLVARDGRVEARPVTTGLSADGFTELRSGVAAGESVVARAGSFLRDGDRVRPVPAATSTP
EEEEECCCEEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
IADAAPR
CCCCCCC
>Mature Secondary Structure
MKTPTCPARERQYPSSADCEVPAVNSVRWRASRLSLLCGLAMLAAAPARAVETAAVPDAA
CCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHH
AIAPAVTVVPAESREIVERAVVTGTLVPRDEILVSPEVEGLRITDLLVEEGDRVTKGQVL
HHCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEHHHHHCCCCCCHHHHH
AKLSLEMIVTQEASNAAAIARAEAAIVQARSQIVQAEAANLEAKQALERAQALAKTGNAT
HHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHH
AAVLEQRVSAAQGAEGRLAAARGGLQSAQADLATARAAGAEIALRRARTDIRAPEAGIVN
HHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
RRTARIGATATAAGEPLFRLIARGEIELEGEVPETSLARIHVGNPASLDLDDGRRLAGKV
CCHHCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCEEEEECCCCCEECCCCCHHHHHHH
RRVYPEVDRATRLGKVRIRLGDDPALRIGAFARGSVEIARRTGVAVPVSSLLYAADGRAS
HHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCHHHHHCCCCCCHHHHHEECCCCEE
VLVARDGRVEARPVTTGLSADGFTELRSGVAAGESVVARAGSFLRDGDRVRPVPAATSTP
EEEEECCCEEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
IADAAPR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]