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The map label for this gene is yjfH [C]

Identifier: 170751421

GI number: 170751421

Start: 5354392

End: 5355267

Strand: Reverse

Name: yjfH [C]

Synonym: Mrad2831_5040

Alternate gene names: 170751421

Gene position: 5355267-5354392 (Counterclockwise)

Preceding gene: 170751422

Following gene: 170751420

Centisome position: 88.11

GC content: 77.4

Gene sequence:

>876_bases
GTGCCCCCGGCCCCGGAGCCCCTCACCGACCCGGACGACCCCCGCCTCGAGCCCTACACCCGGATGCGCGAGCGCGACCT
CGTGGGCCGCGCCGGGCGCTTCATCGTCGAGGGCGAGGTGACCCTGCGGCTGCTGCTCGGCCGCCGCAGCCGGTTCCGCG
CCGAATCGATCCTCCTCGCGCCCGAGCGCTGGCCGGGCCTCGCCGCCGACGTCGCCGCCTGCCCGGAGCCGCCGCCGATC
TACAGCGCCTCCAAGGCGGTGATGAGCGCCGTGACGGGTTTCCCGATCCACCGCGGCGTCCTCGCGGTCGGCCTGCGCGG
GCCGGGGCTCGACGCGGCCGCCCTCGTGCCGCCGCCGCCCGCGCCTGCCCTGGTGGTCGGCCTGGCCGGCCTGACCAATC
ACGACAATGTCGGCGCCGCCTTCCGCAACGCGGCGGCCTTCGGGGCCGACGCCGTTCTCCTCGACGGGGCGACTTGCGAC
CCGCTCTACCGCAAGAGCATCCGCGTCTCGGCCGGGGCCGCCCTGATCCAGCCCTTCGCGCGGCTCCCGGACGGGCAGGA
CTGGCTGCGGCTGGCCGAGACCCTCGACCTCCGGCCGCTCGCCTTCAGTCCGGGGGCGAGCGAGACCCTGGACGCGCGCG
CCGCCCCGCCGCGGGCGCTCCTGATTCTCGGCACCGAGGGCCCCGGCCTCGACGCGGCGACGCTGGCCCGGGCGCGGCCG
ATCCGGATCCCGATGGCCGCCGGCTTCGACTCGCTGAACGTCGCTACCGCGGCGGGCATCGCCCTCTACACCATCGCCCG
CGGCCAGGGGCGCCTCGGCGAACCGCGAGCGCGCCGCGGCGGCGAACCGTTCGACCCGGGGCCGGCAGGTTCTTGA

Upstream 100 bases:

>100_bases
GGCGGCGAGACGGTCCTCGACGGCGAGGCGCTGGTGAAGGTGCCGACCAAGGCCGAGGCCGACCCGCTGGCGAACCGCTC
CGACCGGTCACAGGGCTGAG

Downstream 100 bases:

>100_bases
AGTCAGTATTCCCCGCGTAGACGCCGCGGAACAGGGCCGGAGAACGCGCCTCCAGCTGGAACACATTGACATCTCGACGC
CGCGCGCGGGCGCGCGCAAT

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MPPAPEPLTDPDDPRLEPYTRMRERDLVGRAGRFIVEGEVTLRLLLGRRSRFRAESILLAPERWPGLAADVAACPEPPPI
YSASKAVMSAVTGFPIHRGVLAVGLRGPGLDAAALVPPPPAPALVVGLAGLTNHDNVGAAFRNAAAFGADAVLLDGATCD
PLYRKSIRVSAGAALIQPFARLPDGQDWLRLAETLDLRPLAFSPGASETLDARAAPPRALLILGTEGPGLDAATLARARP
IRIPMAAGFDSLNVATAAGIALYTIARGQGRLGEPRARRGGEPFDPGPAGS

Sequences:

>Translated_291_residues
MPPAPEPLTDPDDPRLEPYTRMRERDLVGRAGRFIVEGEVTLRLLLGRRSRFRAESILLAPERWPGLAADVAACPEPPPI
YSASKAVMSAVTGFPIHRGVLAVGLRGPGLDAAALVPPPPAPALVVGLAGLTNHDNVGAAFRNAAAFGADAVLLDGATCD
PLYRKSIRVSAGAALIQPFARLPDGQDWLRLAETLDLRPLAFSPGASETLDARAAPPRALLILGTEGPGLDAATLARARP
IRIPMAAGFDSLNVATAAGIALYTIARGQGRLGEPRARRGGEPFDPGPAGS
>Mature_290_residues
PPAPEPLTDPDDPRLEPYTRMRERDLVGRAGRFIVEGEVTLRLLLGRRSRFRAESILLAPERWPGLAADVAACPEPPPIY
SASKAVMSAVTGFPIHRGVLAVGLRGPGLDAAALVPPPPAPALVVGLAGLTNHDNVGAAFRNAAAFGADAVLLDGATCDP
LYRKSIRVSAGAALIQPFARLPDGQDWLRLAETLDLRPLAFSPGASETLDARAAPPRALLILGTEGPGLDAATLARARPI
RIPMAAGFDSLNVATAAGIALYTIARGQGRLGEPRARRGGEPFDPGPAGS

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=192, Percent_Identity=28.125, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30335; Mature: 30204

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPAPEPLTDPDDPRLEPYTRMRERDLVGRAGRFIVEGEVTLRLLLGRRSRFRAESILLA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCEEEEEECCCCHHHCCHHEEEC
PERWPGLAADVAACPEPPPIYSASKAVMSAVTGFPIHRGVLAVGLRGPGLDAAALVPPPP
CCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHCCEEEEECCCCCCCCEEECCCCC
APALVVGLAGLTNHDNVGAAFRNAAAFGADAVLLDGATCDPLYRKSIRVSAGAALIQPFA
CCEEEEECCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHCCHHCCHHHHHHHH
RLPDGQDWLRLAETLDLRPLAFSPGASETLDARAAPPRALLILGTEGPGLDAATLARARP
HCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCC
IRIPMAAGFDSLNVATAAGIALYTIARGQGRLGEPRARRGGEPFDPGPAGS
EEEEHHCCCCCCCHHHHHCEEEEEEECCCCCCCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PPAPEPLTDPDDPRLEPYTRMRERDLVGRAGRFIVEGEVTLRLLLGRRSRFRAESILLA
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCEEEEEECCCCHHHCCHHEEEC
PERWPGLAADVAACPEPPPIYSASKAVMSAVTGFPIHRGVLAVGLRGPGLDAAALVPPPP
CCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHCCEEEEECCCCCCCCEEECCCCC
APALVVGLAGLTNHDNVGAAFRNAAAFGADAVLLDGATCDPLYRKSIRVSAGAALIQPFA
CCEEEEECCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHCCHHCCHHHHHHHH
RLPDGQDWLRLAETLDLRPLAFSPGASETLDARAAPPRALLILGTEGPGLDAATLARARP
HCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCC
IRIPMAAGFDSLNVATAAGIALYTIARGQGRLGEPRARRGGEPFDPGPAGS
EEEEHHCCCCCCCHHHHHCEEEEEEECCCCCCCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]