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The map label for this gene is guaA [C]

Identifier: 170751413

GI number: 170751413

Start: 5346772

End: 5347620

Strand: Reverse

Name: guaA [C]

Synonym: Mrad2831_5032

Alternate gene names: 170751413

Gene position: 5347620-5346772 (Counterclockwise)

Preceding gene: 170751416

Following gene: 170751412

Centisome position: 87.99

GC content: 74.09

Gene sequence:

>849_bases
ATGCTCCGCCTGCTCATCGCCGACGGCAACGATCGCGCTGCCCGCGACCGCCACGTCGCGGCCACCGGACGAACCTCGGC
CGAGTCCTACGCGGCGGTGGTGCGGGACCTCGCGCCGGGCTCCGTCTGCCGGCACGTCAACGCCGCCGACGCCGACGCGA
CGCTGCCGGCAGACCTCACGGATTTCGACGGGCTGATCTTCACCGGGTCGACCCTCAAGGTCTCGGAGGGTACGCCGGCC
GTGATCCGGCAGCTCGACCTGATGCGCGCGGCCTTCGAGGCCGGGCGCGCGGTCTTCGGCTCGTGCTGGGGCGTGCAGGT
CGCGGCTACGGTGGCGGGTGGCCATGCCGCCGCCAACCCACGCGGGCCGGAATACGGCTTCGCCCGGGCGATCACCCCGA
CCGAGGAGGGTCGGACACACGCCCTGCTGGCCGGCCGTCCGGCGACCTGGGATGCCCCGGCGATCCACTCCGACGCCGTC
CTGGAGCCGCCCCCCGGCGCCCGCGTTCTCGCCGGCAATCGCGTGCTCGGCATTCAGGCCATCGAGATCCGCCGGGAGCG
CGGCTGGTTCTGGGGCACCCAGTATCATCCCGAACTCGACCTCGACGAACTGGCCGCGATGCTGCGCCTCTGCGACACCG
CCATCGTCGCGGCGGGGCTCGCCGAGAACCCGCGGGCCGTGGCGGCCTACGCCGACGAGATCGCGACGCTCCAGGGACGC
GACCCGGAAGCCGCGCGCCGCCTCGCCTGGCGTCACGGCATCGGGCCGGAGGTGATGGAGGCCGGCCTCCGTCGCCGGGA
GATCGGCAACTTCCTGGCGCGCCTGTCCACAGGCGAGCGCCGCCGGTAG

Upstream 100 bases:

>100_bases
TCGACGCCCGCCTCCCGCGACGATCCGCGCCGTTCCTGCGACGCGGTGCGCACCGACATAGAGGCGCTTCCGCTACGGCC
GCGCTCCCCGGAAATCCCGC

Downstream 100 bases:

>100_bases
GCTGCCGCGACCGGGTGGCGCGTTGACCCGCCCGGCCTCAAGTCTAGCTTCGCCCTGCGGGCCACGCCTTCTTCGGCCCG
GGGAGCCAGCCAGCAGGATG

Product: glutamine amidotransferase class-I

Products: AMP; L-Glutamate; GMP; H+; Diphosphate [C]

Alternate protein names: Glutamine Amidotransferase; Class I Glutamine Amidotransferase Family Protein; Glutamine Amidotransferase Class I; Glutamine-Hydrolyzing GMP Synthase

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MLRLLIADGNDRAARDRHVAATGRTSAESYAAVVRDLAPGSVCRHVNAADADATLPADLTDFDGLIFTGSTLKVSEGTPA
VIRQLDLMRAAFEAGRAVFGSCWGVQVAATVAGGHAAANPRGPEYGFARAITPTEEGRTHALLAGRPATWDAPAIHSDAV
LEPPPGARVLAGNRVLGIQAIEIRRERGWFWGTQYHPELDLDELAAMLRLCDTAIVAAGLAENPRAVAAYADEIATLQGR
DPEAARRLAWRHGIGPEVMEAGLRRREIGNFLARLSTGERRR

Sequences:

>Translated_282_residues
MLRLLIADGNDRAARDRHVAATGRTSAESYAAVVRDLAPGSVCRHVNAADADATLPADLTDFDGLIFTGSTLKVSEGTPA
VIRQLDLMRAAFEAGRAVFGSCWGVQVAATVAGGHAAANPRGPEYGFARAITPTEEGRTHALLAGRPATWDAPAIHSDAV
LEPPPGARVLAGNRVLGIQAIEIRRERGWFWGTQYHPELDLDELAAMLRLCDTAIVAAGLAENPRAVAAYADEIATLQGR
DPEAARRLAWRHGIGPEVMEAGLRRREIGNFLARLSTGERRR
>Mature_282_residues
MLRLLIADGNDRAARDRHVAATGRTSAESYAAVVRDLAPGSVCRHVNAADADATLPADLTDFDGLIFTGSTLKVSEGTPA
VIRQLDLMRAAFEAGRAVFGSCWGVQVAATVAGGHAAANPRGPEYGFARAITPTEEGRTHALLAGRPATWDAPAIHSDAV
LEPPPGARVLAGNRVLGIQAIEIRRERGWFWGTQYHPELDLDELAAMLRLCDTAIVAAGLAENPRAVAAYADEIATLQGR
DPEAARRLAWRHGIGPEVMEAGLRRREIGNFLARLSTGERRR

Specific function: Catalyzes The Synthesis Of Gmp From Xmp. [C]

COG id: COG0518

COG function: function code F; GMP synthase - Glutamine amidotransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.5.2 [C]

Molecular weight: Translated: 30151; Mature: 30151

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLLIADGNDRAARDRHVAATGRTSAESYAAVVRDLAPGSVCRHVNAADADATLPADLT
CEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCC
DFDGLIFTGSTLKVSEGTPAVIRQLDLMRAAFEAGRAVFGSCWGVQVAATVAGGHAAANP
CCCCEEEECCEEEECCCCHHHHHHHHHHHHHHHHCHHHHHHCCCEEEEEEEECCCCCCCC
RGPEYGFARAITPTEEGRTHALLAGRPATWDAPAIHSDAVLEPPPGARVLAGNRVLGIQA
CCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEE
IEIRRERGWFWGTQYHPELDLDELAAMLRLCDTAIVAAGLAENPRAVAAYADEIATLQGR
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
DPEAARRLAWRHGIGPEVMEAGLRRREIGNFLARLSTGERRR
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLRLLIADGNDRAARDRHVAATGRTSAESYAAVVRDLAPGSVCRHVNAADADATLPADLT
CEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCC
DFDGLIFTGSTLKVSEGTPAVIRQLDLMRAAFEAGRAVFGSCWGVQVAATVAGGHAAANP
CCCCEEEECCEEEECCCCHHHHHHHHHHHHHHHHCHHHHHHCCCEEEEEEEECCCCCCCC
RGPEYGFARAITPTEEGRTHALLAGRPATWDAPAIHSDAVLEPPPGARVLAGNRVLGIQA
CCCCCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEE
IEIRRERGWFWGTQYHPELDLDELAAMLRLCDTAIVAAGLAENPRAVAAYADEIATLQGR
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
DPEAARRLAWRHGIGPEVMEAGLRRREIGNFLARLSTGERRR
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1 {NH4+}} 0.42 {6-thioXMP}} 0.53 {ATP}} 2 {1-ribosyl-4,6-dihydroxypyrazolo[3,4-d]-pyrimidine} 0.058 {XMP}} 0.029 {XMP}} 0.35 {8-azaXMP}} [C]

Substrates: ATP; L-Glutamine; H2O; Xanthosine 5'-phosphate [C]

Specific reaction: ATP + L-Glutamine + H2O + Xanthosine 5'-phosphate --> AMP + L-Glutamate + GMP + (2) H+ + Diphosphate [C]

General reaction: Amination [C]

Inhibitor: 1, N6-Ethenoadenosine; 1-Methyl adenosine 5'-phosphate 5'-phosphate; 2', 3'-Dihydroadenosine; 2'-Deoxyadenosine; 2, 6-Diaminopurine ribonucleoside; 2-Amino-6-methyl thiopurine ribonucleoside; 2-Fluoroadenosine; 3'-Deoxyadenosine; 5'-Azido-5'-deoxyadenosin

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA