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The map label for this gene is pcm [H]

Identifier: 170748698

GI number: 170748698

Start: 2411106

End: 2411753

Strand: Reverse

Name: pcm [H]

Synonym: Mrad2831_2280

Alternate gene names: 170748698

Gene position: 2411753-2411106 (Counterclockwise)

Preceding gene: 170748699

Following gene: 170748691

Centisome position: 39.68

GC content: 76.85

Gene sequence:

>648_bases
GTGATCGATCTGGGCAGCCTGGAGGAGGCTGCCGGCAACGCGGCCTTCGTGATGGCGCTCCGCGGCCGTGGCCTGCGGGA
CACCGCGGTGCTGCGGGCCATGGAGCAGGTCCCGCGGGACACCTTCGCGCCGCCGCCGCATCGGGAGCACGCGCGGCGCG
ACATCGCCCTGCCGCTGTCCTGCGGCGCGTCGATGACGGCGCCGTCCACGGTGGCGCTGATGCTGGCGCAGCTGCGGCTG
GAGCCCGGCCAGCGCGTCCTGGAGATCGGCACGGGCTCGGGCTACGTGACCGCGCTGCTCGCCCAGCTGGGCGCGGGGGC
GGTGCTCAGCCTGGAACGCTACACCTCGCTGGCCGGGGCGGCGACGCGGCGGCTCGCGGCCCTGTCCGAGGTGCATGTCG
TCCGGGCGGACGGGCTCGCCCCGGACCTGCCGGACGGCGGCTTCGACCGCATCCTCGTCCACGGCAGCGTCCCGGCGATC
CCGCCGCACTGGCGCGCGGCCCTCGAACCGGGCGGACGGCTGGTGACCGGCATGGGCGGCGGCGGAGCTTGCCGGATCGC
GGTGCTGACGACGCCCGAGGCCGACCCGGAACCGGGCGCGCCGGTGCGTCTCGCAGCGCTGGTGCCGGGTCTGGCGCGCG
TGCTCTGA

Upstream 100 bases:

>100_bases
ATCTCAAGGCCATCGCCGAGAAGCGGATCTCGGTGACGCCCCTGCGCCTCGACCTCACCGACGAGCCGACGCTGACGCGC
TTCGCGCAGGCCTTCGCCGA

Downstream 100 bases:

>100_bases
CGACGCGCCGTCATCGCGAGCGCAGCCAAGCGACCCAGGGTAGCGCACCGCGTTCGCGAGCGTGGCGCCGCTGGATCGCG
CCGCTCCGCTCGCGAAGACG

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MIDLGSLEEAAGNAAFVMALRGRGLRDTAVLRAMEQVPRDTFAPPPHREHARRDIALPLSCGASMTAPSTVALMLAQLRL
EPGQRVLEIGTGSGYVTALLAQLGAGAVLSLERYTSLAGAATRRLAALSEVHVVRADGLAPDLPDGGFDRILVHGSVPAI
PPHWRAALEPGGRLVTGMGGGGACRIAVLTTPEADPEPGAPVRLAALVPGLARVL

Sequences:

>Translated_215_residues
MIDLGSLEEAAGNAAFVMALRGRGLRDTAVLRAMEQVPRDTFAPPPHREHARRDIALPLSCGASMTAPSTVALMLAQLRL
EPGQRVLEIGTGSGYVTALLAQLGAGAVLSLERYTSLAGAATRRLAALSEVHVVRADGLAPDLPDGGFDRILVHGSVPAI
PPHWRAALEPGGRLVTGMGGGGACRIAVLTTPEADPEPGAPVRLAALVPGLARVL
>Mature_215_residues
MIDLGSLEEAAGNAAFVMALRGRGLRDTAVLRAMEQVPRDTFAPPPHREHARRDIALPLSCGASMTAPSTVALMLAQLRL
EPGQRVLEIGTGSGYVTALLAQLGAGAVLSLERYTSLAGAATRRLAALSEVHVVRADGLAPDLPDGGFDRILVHGSVPAI
PPHWRAALEPGGRLVTGMGGGGACRIAVLTTPEADPEPGAPVRLAALVPGLARVL

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=167, Percent_Identity=40.1197604790419, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 22175; Mature: 22175

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLGSLEEAAGNAAFVMALRGRGLRDTAVLRAMEQVPRDTFAPPPHREHARRDIALPLS
CCCCCCCHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCEEEEC
CGASMTAPSTVALMLAQLRLEPGQRVLEIGTGSGYVTALLAQLGAGAVLSLERYTSLAGA
CCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEHHHHHHHHHH
ATRRLAALSEVHVVRADGLAPDLPDGGFDRILVHGSVPAIPPHWRAALEPGGRLVTGMGG
HHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCEEEEECCC
GGACRIAVLTTPEADPEPGAPVRLAALVPGLARVL
CCEEEEEEEECCCCCCCCCCCEEHHHHHHHHHHHC
>Mature Secondary Structure
MIDLGSLEEAAGNAAFVMALRGRGLRDTAVLRAMEQVPRDTFAPPPHREHARRDIALPLS
CCCCCCCHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCEEEEC
CGASMTAPSTVALMLAQLRLEPGQRVLEIGTGSGYVTALLAQLGAGAVLSLERYTSLAGA
CCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEHHHHHHHHHH
ATRRLAALSEVHVVRADGLAPDLPDGGFDRILVHGSVPAIPPHWRAALEPGGRLVTGMGG
HHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCEEEEECCC
GGACRIAVLTTPEADPEPGAPVRLAALVPGLARVL
CCEEEEEEEECCCCCCCCCCCEEHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA