Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 170748681

GI number: 170748681

Start: 2395603

End: 2396835

Strand: Reverse

Name: gatA [H]

Synonym: Mrad2831_2263

Alternate gene names: 170748681

Gene position: 2396835-2395603 (Counterclockwise)

Preceding gene: 170748687

Following gene: 170748679

Centisome position: 39.44

GC content: 75.91

Gene sequence:

>1233_bases
ATGCTCTCGCTTCTCGACATCCGCGCCCGCATCGAAGCCGGCACCCTGACGCCCGCGGGCGCGATCGACCTGTGCCGCGC
CGCGATCGCCGCGCGGGAGCCGGACCTGCACGCGCTGGTCACCCTCGATCCGGCTCCGGCGATCCCCGAGACCGGGCCGC
TCGCCGGCATCGCGGTCGGCGTGAAGGACATCATCGACAGCGCGGGCCTGCCGAGCCAGATGGGCTCGCCGATCTACGCG
GGCTGGATGCCCCGGGGCGACGCCGCGGTCGTGAGCCGGCTGAAGCAGCTCGGGGCCGTCGCCCTCGCCAAGACCACCAC
GACGGCCTTCGCGAGCAGCGATCCCACCGAGACGGTCAACCCGCACGATCCCGGCCACACGCCGGGGGGCTCCTCGGCGG
GCTCGGCCGCCGCCGTCGGCGCCGGGATGCTGCCGCTGGCCCTGGGCACGCAGACGGCGGGCTCGGTGATCCGGCCCGCG
AGCTTCTGCGGCTGCGCGGCGGTGAAGCCGTCGTTCCGGCTGATCCCCACGGTCGGCGTGAAGACCTACTCGTGGGCCCT
CGACACGGTCGGGCTGTTCGCCGCCGGGGTCGCGGACGTCGCGCACGCCCTCGCGCTCGTCACCGGGCGGCCGGAGATCG
AGCGGGCGGAGGCGGGCTCGGCGCCGCGCATCGCCGTGGTGCGGCAGGACTTCGCCGGGGACCCCGAGCCGGAGGCCGAG
GCCGCGCTCACCCGCGCCCGGCAGGCGGCCGAGCGGTCCGGGGCGCGGGCGAGCGACCTCGTCCTGCCGCAGGAGCTGGC
ACAGGCCTGGGAGCGCCACCGTCTCATCCAGAACTACGAGGGCCGGCTGGCGCTGGCCTGGGAGTACGACACGCAGGCCG
ACGCGCTCCCGCCCCGGACCCGCGCGCACCTCGATGACGGGGCGGCGATCAGCCCGGCCCAGTACGACGACGCGCGCCGC
CACGGCCATCACGCCCGGCGGGTGCTGAAGGGCCTCTTCGCCGATTACGACGCGATCCTGACCTTCTCGGCGCCGGGCCC
GGCACCCGCGACCCTGGTCTCGACCGGCGACCCGCGCTTCAACCAGCTGTGGACGCTGATGGGCGTGCCCTGCGTGAACG
TGCCGGTGCCCGGCCACCGCCTGCCGGTGGGCGTCCAGGTGATCGGCCGCTTCGGCGACGACGGCCGAGCCCTGGCGGTG
GCCCGGATGATCGAGGCCGCCCTGCGCGGCTGA

Upstream 100 bases:

>100_bases
TGCGCACGGTCGCGTAAAGCGTTCGTCAACGGCGTTTCGGGAATGCCGCGGGCTGTGGCGTCTCGGTTGACAGCGCCGCG
GACCCCGGACTGAATGCATT

Downstream 100 bases:

>100_bases
CCGGCCGCGCCCGTCGACGCGGCCACGCGCCGTCCGTCCGGGACTGCGGAGCGGAGCCCGGACGGACGGCGCGGGAGCCC
GGGACGCTCAGTGGCTCGGC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MLSLLDIRARIEAGTLTPAGAIDLCRAAIAAREPDLHALVTLDPAPAIPETGPLAGIAVGVKDIIDSAGLPSQMGSPIYA
GWMPRGDAAVVSRLKQLGAVALAKTTTTAFASSDPTETVNPHDPGHTPGGSSAGSAAAVGAGMLPLALGTQTAGSVIRPA
SFCGCAAVKPSFRLIPTVGVKTYSWALDTVGLFAAGVADVAHALALVTGRPEIERAEAGSAPRIAVVRQDFAGDPEPEAE
AALTRARQAAERSGARASDLVLPQELAQAWERHRLIQNYEGRLALAWEYDTQADALPPRTRAHLDDGAAISPAQYDDARR
HGHHARRVLKGLFADYDAILTFSAPGPAPATLVSTGDPRFNQLWTLMGVPCVNVPVPGHRLPVGVQVIGRFGDDGRALAV
ARMIEAALRG

Sequences:

>Translated_410_residues
MLSLLDIRARIEAGTLTPAGAIDLCRAAIAAREPDLHALVTLDPAPAIPETGPLAGIAVGVKDIIDSAGLPSQMGSPIYA
GWMPRGDAAVVSRLKQLGAVALAKTTTTAFASSDPTETVNPHDPGHTPGGSSAGSAAAVGAGMLPLALGTQTAGSVIRPA
SFCGCAAVKPSFRLIPTVGVKTYSWALDTVGLFAAGVADVAHALALVTGRPEIERAEAGSAPRIAVVRQDFAGDPEPEAE
AALTRARQAAERSGARASDLVLPQELAQAWERHRLIQNYEGRLALAWEYDTQADALPPRTRAHLDDGAAISPAQYDDARR
HGHHARRVLKGLFADYDAILTFSAPGPAPATLVSTGDPRFNQLWTLMGVPCVNVPVPGHRLPVGVQVIGRFGDDGRALAV
ARMIEAALRG
>Mature_410_residues
MLSLLDIRARIEAGTLTPAGAIDLCRAAIAAREPDLHALVTLDPAPAIPETGPLAGIAVGVKDIIDSAGLPSQMGSPIYA
GWMPRGDAAVVSRLKQLGAVALAKTTTTAFASSDPTETVNPHDPGHTPGGSSAGSAAAVGAGMLPLALGTQTAGSVIRPA
SFCGCAAVKPSFRLIPTVGVKTYSWALDTVGLFAAGVADVAHALALVTGRPEIERAEAGSAPRIAVVRQDFAGDPEPEAE
AALTRARQAAERSGARASDLVLPQELAQAWERHRLIQNYEGRLALAWEYDTQADALPPRTRAHLDDGAAISPAQYDDARR
HGHHARRVLKGLFADYDAILTFSAPGPAPATLVSTGDPRFNQLWTLMGVPCVNVPVPGHRLPVGVQVIGRFGDDGRALAV
ARMIEAALRG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=496, Percent_Identity=24.3951612903226, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=461, Percent_Identity=24.295010845987, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17556264, Length=184, Percent_Identity=31.5217391304348, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=151, Percent_Identity=35.0993377483444, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=160, Percent_Identity=37.5, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=465, Percent_Identity=24.0860215053763, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550774, Length=419, Percent_Identity=24.3436754176611, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648435, Length=419, Percent_Identity=24.3436754176611, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648437, Length=419, Percent_Identity=24.3436754176611, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648441, Length=409, Percent_Identity=24.2053789731051, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648439, Length=409, Percent_Identity=24.2053789731051, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648113, Length=428, Percent_Identity=25.9345794392523, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 42555; Mature: 42555

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLLDIRARIEAGTLTPAGAIDLCRAAIAAREPDLHALVTLDPAPAIPETGPLAGIAVG
CCCCEEHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHH
VKDIIDSAGLPSQMGSPIYAGWMPRGDAAVVSRLKQLGAVALAKTTTTAFASSDPTETVN
HHHHHHCCCCCHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PHDPGHTPGGSSAGSAAAVGAGMLPLALGTQTAGSVIRPASFCGCAAVKPSFRLIPTVGV
CCCCCCCCCCCCCCCHHHHCCCCHHHHHCCCCCCCCCCCHHHCCHHHCCCCCEEEEECCC
KTYSWALDTVGLFAAGVADVAHALALVTGRPEIERAEAGSAPRIAVVRQDFAGDPEPEAE
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEEEEHHCCCCCCCHHH
AALTRARQAAERSGARASDLVLPQELAQAWERHRLIQNYEGRLALAWEYDTQADALPPRT
HHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCH
RAHLDDGAAISPAQYDDARRHGHHARRVLKGLFADYDAILTFSAPGPAPATLVSTGDPRF
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCCH
NQLWTLMGVPCVNVPVPGHRLPVGVQVIGRFGDDGRALAVARMIEAALRG
HHHHHHHCCCEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSLLDIRARIEAGTLTPAGAIDLCRAAIAAREPDLHALVTLDPAPAIPETGPLAGIAVG
CCCCEEHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHH
VKDIIDSAGLPSQMGSPIYAGWMPRGDAAVVSRLKQLGAVALAKTTTTAFASSDPTETVN
HHHHHHCCCCCHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PHDPGHTPGGSSAGSAAAVGAGMLPLALGTQTAGSVIRPASFCGCAAVKPSFRLIPTVGV
CCCCCCCCCCCCCCCHHHHCCCCHHHHHCCCCCCCCCCCHHHCCHHHCCCCCEEEEECCC
KTYSWALDTVGLFAAGVADVAHALALVTGRPEIERAEAGSAPRIAVVRQDFAGDPEPEAE
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEEEEHHCCCCCCCHHH
AALTRARQAAERSGARASDLVLPQELAQAWERHRLIQNYEGRLALAWEYDTQADALPPRT
HHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCH
RAHLDDGAAISPAQYDDARRHGHHARRVLKGLFADYDAILTFSAPGPAPATLVSTGDPRF
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCCCH
NQLWTLMGVPCVNVPVPGHRLPVGVQVIGRFGDDGRALAVARMIEAALRG
HHHHHHHCCCEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA