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The map label for this gene is ydgK [H]

Identifier: 170748520

GI number: 170748520

Start: 2232403

End: 2233653

Strand: Reverse

Name: ydgK [H]

Synonym: Mrad2831_2102

Alternate gene names: 170748520

Gene position: 2233653-2232403 (Counterclockwise)

Preceding gene: 170748521

Following gene: 170748519

Centisome position: 36.75

GC content: 77.06

Gene sequence:

>1251_bases
TTGGGCCTGACCCGCGCCGCCCCGGCGCATCCCCCCATCCGCCCCGGCTCGGCCGCCTTCTCGGCGCTGCTCGGGCTGCT
GGCGGCGCTCCCCACCTTCGGGATCGACATGATCCTGCCGAGCCTGTCGGACACGGCGGCCGCGCTGCATGTCCCGGCCA
CCGCGATGGGCGCGGCTATGGGCGTCTACCTGCTCGGCTTCGGCGGCGCGCTGCTGGTCTACGGTCCGGTCTCGGACCGG
CTGGGCCGCAAGCCGGCCATCGTCTGCGGCTGCCTCCTCCTCGTCGTCGGCAGCCTCGGCTGCGGCGCGGCGCGGACGCT
GCCGGACTTCCTGCTGTTCCGGGCCGTGCAGGGGATCGGCGCGGCGGGCCCGGGCATGGCGGTCCTCGCGATGGTGCGCG
ACCTGTTCGAGGGCGAGGCGGCGCGCGCCCGGATGTCGTTCGTCGTCCTCACCATCAACGTGGTGCCGACGGTGGCCCCG
ACCCTCGGCGCCGGTCTGATGCATCTCGGCGGCTGGCGCCTGATCCACGCCGCGCCGGTCGCCGCCGCCCTGGCGGCCCT
GGTGGCGATCCGGCACGTCGACGAGCCCCTGCGGCGCGCCGCGGATCCCGGGCCGGCCTCCGGGATCCTGCGCGGCTACG
GGCGGATTCTGGCGAGCCGGGTCTTCCTCGGCCACGCCCTGTGCAACGCCGCGGCGGCGGGGGCGGTCTTCGCCTACATC
ACCGGCTCGGCGCTGGTCTTCGTCGACGCCCTCGGCCTCGACCCGCTGGCCTACGGGCTGGTCTTCGGGGCGAGCTCCCT
GTCGGTGATGGCGGGCGCCTTCCTGAACGGGCGGCTCGCGGCCCGGGGCGTCCCCCCGCCGCGGGTGATCGCCGCCGGCC
TCGCCCTGGCGACGGGCGCGGCGGCCGCGCTCCTCGTCGCCGCGCTGGCCCGGCCGCCCGCGGCCCTGCCGGTGGTCGCC
GCGATGGTCGGCGTGGCCCTGGCCTTCGGCCTCGTCTCGCCCAACGCCATGCACGGCGCGACGGAGCGCAACCCGGAGGA
CGCGGGCGCGGCCAGCGCCGTCGCGGTCTTCCTGCAGATGCTCGCGGCGGCCATCGCCAGCGATCTCGTGGCCCGGTTCT
TCGACGGCCGGTCCGCCCTGTCGATGGCCGCCGCGATGCTCGGCTGCTGCCTCACGGCCGCCGCCGCCCACGCGGGTCTC
GCCCGATCGGCACCCCGCGCCCCCTCCCAGCCCCACGGAGAACCCGCATGA

Upstream 100 bases:

>100_bases
CGGATCTCGACGCGGGCGGCCTCGTCCGCGAGATCGACGCGATGGTGGCGGCGCGCCGGGATCCCGGGGCCCGGGGTGTC
GCTCGGGGCGGAGACCGGCT

Downstream 100 bases:

>100_bases
TCTTCGACCCGCCCCCCTGGCCGCCCCTCTACCTGACCCGCGAGGACGGCCGGCTCGCCCATATCGAGGCCGGGCCGCTC
ACCCCCGAGGGGCAGCCGCT

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MGLTRAAPAHPPIRPGSAAFSALLGLLAALPTFGIDMILPSLSDTAAALHVPATAMGAAMGVYLLGFGGALLVYGPVSDR
LGRKPAIVCGCLLLVVGSLGCGAARTLPDFLLFRAVQGIGAAGPGMAVLAMVRDLFEGEAARARMSFVVLTINVVPTVAP
TLGAGLMHLGGWRLIHAAPVAAALAALVAIRHVDEPLRRAADPGPASGILRGYGRILASRVFLGHALCNAAAAGAVFAYI
TGSALVFVDALGLDPLAYGLVFGASSLSVMAGAFLNGRLAARGVPPPRVIAAGLALATGAAAALLVAALARPPAALPVVA
AMVGVALAFGLVSPNAMHGATERNPEDAGAASAVAVFLQMLAAAIASDLVARFFDGRSALSMAAAMLGCCLTAAAAHAGL
ARSAPRAPSQPHGEPA

Sequences:

>Translated_416_residues
MGLTRAAPAHPPIRPGSAAFSALLGLLAALPTFGIDMILPSLSDTAAALHVPATAMGAAMGVYLLGFGGALLVYGPVSDR
LGRKPAIVCGCLLLVVGSLGCGAARTLPDFLLFRAVQGIGAAGPGMAVLAMVRDLFEGEAARARMSFVVLTINVVPTVAP
TLGAGLMHLGGWRLIHAAPVAAALAALVAIRHVDEPLRRAADPGPASGILRGYGRILASRVFLGHALCNAAAAGAVFAYI
TGSALVFVDALGLDPLAYGLVFGASSLSVMAGAFLNGRLAARGVPPPRVIAAGLALATGAAAALLVAALARPPAALPVVA
AMVGVALAFGLVSPNAMHGATERNPEDAGAASAVAVFLQMLAAAIASDLVARFFDGRSALSMAAAMLGCCLTAAAAHAGL
ARSAPRAPSQPHGEPA
>Mature_415_residues
GLTRAAPAHPPIRPGSAAFSALLGLLAALPTFGIDMILPSLSDTAAALHVPATAMGAAMGVYLLGFGGALLVYGPVSDRL
GRKPAIVCGCLLLVVGSLGCGAARTLPDFLLFRAVQGIGAAGPGMAVLAMVRDLFEGEAARARMSFVVLTINVVPTVAPT
LGAGLMHLGGWRLIHAAPVAAALAALVAIRHVDEPLRRAADPGPASGILRGYGRILASRVFLGHALCNAAAAGAVFAYIT
GSALVFVDALGLDPLAYGLVFGASSLSVMAGAFLNGRLAARGVPPPRVIAAGLALATGAAAALLVAALARPPAALPVVAA
MVGVALAFGLVSPNAMHGATERNPEDAGAASAVAVFLQMLAAAIASDLVARFFDGRSALSMAAAMLGCCLTAAAAHAGLA
RSAPRAPSQPHGEPA

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=349, Percent_Identity=27.5071633237822, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI48994889, Length=408, Percent_Identity=25.2450980392157, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790794, Length=365, Percent_Identity=23.013698630137, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790146, Length=228, Percent_Identity=28.0701754385965, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082312, Length=349, Percent_Identity=27.2206303724928, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787065, Length=349, Percent_Identity=26.647564469914, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41228; Mature: 41097

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTRAAPAHPPIRPGSAAFSALLGLLAALPTFGIDMILPSLSDTAAALHVPATAMGAAM
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHH
GVYLLGFGGALLVYGPVSDRLGRKPAIVCGCLLLVVGSLGCGAARTLPDFLLFRAVQGIG
HHHHHHCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
AAGPGMAVLAMVRDLFEGEAARARMSFVVLTINVVPTVAPTLGAGLMHLGGWRLIHAAPV
CCCCHHHHHHHHHHHHCCHHHHHHHEEEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHH
AAALAALVAIRHVDEPLRRAADPGPASGILRGYGRILASRVFLGHALCNAAAAGAVFAYI
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGSALVFVDALGLDPLAYGLVFGASSLSVMAGAFLNGRLAARGVPPPRVIAAGLALATGA
HCCHHEEHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHH
AAALLVAALARPPAALPVVAAMVGVALAFGLVSPNAMHGATERNPEDAGAASAVAVFLQM
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHHH
LAAAIASDLVARFFDGRSALSMAAAMLGCCLTAAAAHAGLARSAPRAPSQPHGEPA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
GLTRAAPAHPPIRPGSAAFSALLGLLAALPTFGIDMILPSLSDTAAALHVPATAMGAAM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHH
GVYLLGFGGALLVYGPVSDRLGRKPAIVCGCLLLVVGSLGCGAARTLPDFLLFRAVQGIG
HHHHHHCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
AAGPGMAVLAMVRDLFEGEAARARMSFVVLTINVVPTVAPTLGAGLMHLGGWRLIHAAPV
CCCCHHHHHHHHHHHHCCHHHHHHHEEEEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHH
AAALAALVAIRHVDEPLRRAADPGPASGILRGYGRILASRVFLGHALCNAAAAGAVFAYI
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGSALVFVDALGLDPLAYGLVFGASSLSVMAGAFLNGRLAARGVPPPRVIAAGLALATGA
HCCHHEEHHHHCCCHHHHHHHHCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHH
AAALLVAALARPPAALPVVAAMVGVALAFGLVSPNAMHGATERNPEDAGAASAVAVFLQM
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHHH
LAAAIASDLVARFFDGRSALSMAAAMLGCCLTAAAAHAGLARSAPRAPSQPHGEPA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]