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The map label for this gene is nuoF1 [H]

Identifier: 170748473

GI number: 170748473

Start: 2185408

End: 2186709

Strand: Reverse

Name: nuoF1 [H]

Synonym: Mrad2831_2055

Alternate gene names: 170748473

Gene position: 2186709-2185408 (Counterclockwise)

Preceding gene: 170748474

Following gene: 170748472

Centisome position: 35.98

GC content: 67.97

Gene sequence:

>1302_bases
ATGCTGTCGGATCAGGATCGCATCTTCACCAACCTCTACGGGCTGCACTCGCCGGGCCTGGAGGCCGCCAAGAAGCGCGG
CGCGTGGGACGGGACCAAGTTCCTCCTGGAGCAGGGCCGCGACTGGATCATCGAGGAGATGAAGGCCTCGGGCCTGCGCG
GCCGCGGCGGCGCGGGCTTCCCCACCGGCCTCAAGTGGTCGTTCATGCCCAAGAAGTCCGACGGGCGCCCGCACTACCTC
GTGGTCAACGCCGACGAGTCGGAGCCGGGCACCTGCAAGGACCGGGAGATCATGCGGCACGACCCGCATCTCCTGATCGA
AGGCTGCATGCTGGCGTGTTTCGCGATGAACGCGCATGCCTGCTACATCTACATCCGCGGCGAGTACGTCGCCGAGAAGC
ACGCGCTCCAGAAGGCCGTGGACGAGGCCTACGCGGCCCGGCTCGTCGGCCAGTCGAACGTGCACGACTACCCGTTCGAC
ATCTACGTCCACCACGGCGCGGGCGCCTATATCTGCGGCGAGGAGACGGCGCTCATCGAGAGCCTGGAGGGCAAGAAGGG
GATGCCGCGGCTGAAGCCGCCGTTCCCCGCCAATATGGGCCTCTACGGCTGCCCCACGACCGTGAACAACGTCGAGTCGA
TCGCGGTCGCCGGCACGATCCTGCGCCGGGGCGGCGCGTGGTTCGCGGGGCTCGGCGGCAAGAACAACACCGGCACGAAG
CTCTTCTGCGTCTCCGGCCACGTCAACAAGCCGTGCAACGTCGAGGAGGAGCTCGGCATCACCTTCCGCGAGCTCATCGA
CCGCCATTGCGGCGGCATGCGCGGCGGCTGGGACAACCTGCTGTGCTCGATCCCGGGCGGCTCCTCGGTGCCGCTGGTGC
CGGCCGAGCAGATCGTCGACGCCAAGATGGACTTCGACACCCTGCGCAACCTGGGTTCCGGCCTCGGCACGGCGGCGGTG
ATCGTCCTCGACAAGTCCACCGACATCGTCGCGGCGATCACGCGGATCTCGTACTTCTACAAGCACGAGTCCTGCGGCCA
GTGCACGCCCTGCCGCGAGGGCACCGGCTGGATGTGGCGGGTGATGCAGCGCATGACCGAGGGCCGCGCGCAGAAGCGCG
AGATCGACATGCTGTTCGAGGTCACCAAGCAGATCGAGGGTCACACGATCTGCGCGCTCGGCGACGCGGCGGCCTGGCCG
ATCCAGGGCCTGATCCGGCACTTCCGCCCGGAGATCGAGAAGCGCATCGACCGCTACACCGCCAACCCGCACAGCGAGCC
TGTGCCGATGGCCGCGGAGTGA

Upstream 100 bases:

>100_bases
CTGGGTCGGCACCTACCTCGCGTTCCCGGGTCGCATCGACCGCGAGAACTGGGTGGGGCAGGCCAAGGCCCTGTCGGCGC
CGGCTCAGTGAGGACCTGAC

Downstream 100 bases:

>100_bases
TCCGATGACGAGCCAGCCTGACAACCTGATGCTCGTGTACCTGCGGCGGATCGACGAGAAGGTCGATCGCCTCAGCGATG
ATGTCCGAGAACTGAAAGGC

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 1; NDH-1 subunit F 1 [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MLSDQDRIFTNLYGLHSPGLEAAKKRGAWDGTKFLLEQGRDWIIEEMKASGLRGRGGAGFPTGLKWSFMPKKSDGRPHYL
VVNADESEPGTCKDREIMRHDPHLLIEGCMLACFAMNAHACYIYIRGEYVAEKHALQKAVDEAYAARLVGQSNVHDYPFD
IYVHHGAGAYICGEETALIESLEGKKGMPRLKPPFPANMGLYGCPTTVNNVESIAVAGTILRRGGAWFAGLGGKNNTGTK
LFCVSGHVNKPCNVEEELGITFRELIDRHCGGMRGGWDNLLCSIPGGSSVPLVPAEQIVDAKMDFDTLRNLGSGLGTAAV
IVLDKSTDIVAAITRISYFYKHESCGQCTPCREGTGWMWRVMQRMTEGRAQKREIDMLFEVTKQIEGHTICALGDAAAWP
IQGLIRHFRPEIEKRIDRYTANPHSEPVPMAAE

Sequences:

>Translated_433_residues
MLSDQDRIFTNLYGLHSPGLEAAKKRGAWDGTKFLLEQGRDWIIEEMKASGLRGRGGAGFPTGLKWSFMPKKSDGRPHYL
VVNADESEPGTCKDREIMRHDPHLLIEGCMLACFAMNAHACYIYIRGEYVAEKHALQKAVDEAYAARLVGQSNVHDYPFD
IYVHHGAGAYICGEETALIESLEGKKGMPRLKPPFPANMGLYGCPTTVNNVESIAVAGTILRRGGAWFAGLGGKNNTGTK
LFCVSGHVNKPCNVEEELGITFRELIDRHCGGMRGGWDNLLCSIPGGSSVPLVPAEQIVDAKMDFDTLRNLGSGLGTAAV
IVLDKSTDIVAAITRISYFYKHESCGQCTPCREGTGWMWRVMQRMTEGRAQKREIDMLFEVTKQIEGHTICALGDAAAWP
IQGLIRHFRPEIEKRIDRYTANPHSEPVPMAAE
>Mature_433_residues
MLSDQDRIFTNLYGLHSPGLEAAKKRGAWDGTKFLLEQGRDWIIEEMKASGLRGRGGAGFPTGLKWSFMPKKSDGRPHYL
VVNADESEPGTCKDREIMRHDPHLLIEGCMLACFAMNAHACYIYIRGEYVAEKHALQKAVDEAYAARLVGQSNVHDYPFD
IYVHHGAGAYICGEETALIESLEGKKGMPRLKPPFPANMGLYGCPTTVNNVESIAVAGTILRRGGAWFAGLGGKNNTGTK
LFCVSGHVNKPCNVEEELGITFRELIDRHCGGMRGGWDNLLCSIPGGSSVPLVPAEQIVDAKMDFDTLRNLGSGLGTAAV
IVLDKSTDIVAAITRISYFYKHESCGQCTPCREGTGWMWRVMQRMTEGRAQKREIDMLFEVTKQIEGHTICALGDAAAWP
IQGLIRHFRPEIEKRIDRYTANPHSEPVPMAAE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=419, Percent_Identity=65.6324582338902, Blast_Score=576, Evalue=1e-164,
Organism=Homo sapiens, GI260656005, Length=419, Percent_Identity=65.6324582338902, Blast_Score=575, Evalue=1e-164,
Organism=Escherichia coli, GI1788620, Length=406, Percent_Identity=41.1330049261084, Blast_Score=312, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI17531727, Length=419, Percent_Identity=65.6324582338902, Blast_Score=582, Evalue=1e-166,
Organism=Drosophila melanogaster, GI19920794, Length=416, Percent_Identity=66.8269230769231, Blast_Score=590, Evalue=1e-169,
Organism=Drosophila melanogaster, GI45550452, Length=433, Percent_Identity=61.662817551963, Blast_Score=551, Evalue=1e-157,
Organism=Drosophila melanogaster, GI24653819, Length=421, Percent_Identity=46.3182897862233, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24653817, Length=421, Percent_Identity=42.9928741092637, Blast_Score=332, Evalue=2e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 47731; Mature: 47731

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSDQDRIFTNLYGLHSPGLEAAKKRGAWDGTKFLLEQGRDWIIEEMKASGLRGRGGAGF
CCCCHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCC
PTGLKWSFMPKKSDGRPHYLVVNADESEPGTCKDREIMRHDPHLLIEGCMLACFAMNAHA
CCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCE
CYIYIRGEYVAEKHALQKAVDEAYAARLVGQSNVHDYPFDIYVHHGAGAYICGEETALIE
EEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEECCHHHHHH
SLEGKKGMPRLKPPFPANMGLYGCPTTVNNVESIAVAGTILRRGGAWFAGLGGKNNTGTK
HHCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCE
LFCVSGHVNKPCNVEEELGITFRELIDRHCGGMRGGWDNLLCSIPGGSSVPLVPAEQIVD
EEEEECCCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHHH
AKMDFDTLRNLGSGLGTAAVIVLDKSTDIVAAITRISYFYKHESCGQCTPCREGTGWMWR
HHCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
VMQRMTEGRAQKREIDMLFEVTKQIEGHTICALGDAAAWPIQGLIRHFRPEIEKRIDRYT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
ANPHSEPVPMAAE
CCCCCCCCCCCCC
>Mature Secondary Structure
MLSDQDRIFTNLYGLHSPGLEAAKKRGAWDGTKFLLEQGRDWIIEEMKASGLRGRGGAGF
CCCCHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCC
PTGLKWSFMPKKSDGRPHYLVVNADESEPGTCKDREIMRHDPHLLIEGCMLACFAMNAHA
CCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCE
CYIYIRGEYVAEKHALQKAVDEAYAARLVGQSNVHDYPFDIYVHHGAGAYICGEETALIE
EEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEECCHHHHHH
SLEGKKGMPRLKPPFPANMGLYGCPTTVNNVESIAVAGTILRRGGAWFAGLGGKNNTGTK
HHCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCE
LFCVSGHVNKPCNVEEELGITFRELIDRHCGGMRGGWDNLLCSIPGGSSVPLVPAEQIVD
EEEEECCCCCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHHH
AKMDFDTLRNLGSGLGTAAVIVLDKSTDIVAAITRISYFYKHESCGQCTPCREGTGWMWR
HHCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
VMQRMTEGRAQKREIDMLFEVTKQIEGHTICALGDAAAWPIQGLIRHFRPEIEKRIDRYT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHC
ANPHSEPVPMAAE
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]