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The map label for this gene is ywnE [H]

Identifier: 170747162

GI number: 170747162

Start: 768695

End: 770131

Strand: Reverse

Name: ywnE [H]

Synonym: Mrad2831_0728

Alternate gene names: 170747162

Gene position: 770131-768695 (Counterclockwise)

Preceding gene: 170747163

Following gene: 170747160

Centisome position: 12.67

GC content: 70.7

Gene sequence:

>1437_bases
TTGGAAAACGCGATCACCCGCTGGGTCCAGTCGACCGCCCTGATGCGGTCGGATGTCGAGGCCCTGCTCGGCTTCGCGCT
GGCGATCGGTGTCACCCTGCACGCCCTGCTGCGCAAGCGCCGGGTCAGCGTGGCGGTGGGCTGGATCGGGCTCGCGTGGC
TGTCGCCGATCTTCGGTACCGCCCTGTACCTGACCTTCGGCATCAACCGCGTCTCGCGCCGCGCGCGGCGGCTGCGGACC
AAGCCCTCCGACGCGACGCAGCTGCCCGACACCGACGACGCCGTGGTGCCGGAGGCGCTCTGGCCCCTCGACCGGGCGAT
CCGGCGGATCACCGGCCTGCCGGCCTTCGCCGGCAACACCGTGCAGATGTTCCGCAACGGCGACGCGGCCTACCCGGTGA
TGCTCGCGGCGATCCGGGAGGCGCGGACCAGCATCGGTCTGTCGAGCTACATCTTCCGGGACGACGCCACCGGCCGCGAG
TTCTGCGACGCGCTGGTCGAGGCGCAGGCCCGGGGCGTGAAGGTCCGCGTCATCATCGACGGGATCGGCGGCGGCTACTT
CCGGGCGCCGGTCTACCGCCGGCTGACGGCAGCCGCGGTGCCGGCGGCCCTGTTCATGCACTCCGCCCTCCCCTGGCGGA
TGCCGTTCCTCAACCTGCGCTCGCACAAGAAATTGCTGGTCATCGACGGGCGGATCGCCTTCACCGGCGGCCTCAACATC
TCCCACCCGAACCGCGTCGCCCTGAAGCCCGAGCACCCGATCCGCGACACCCATTTCCGGATGACCGGCCCCGTGGTCGA
GCAGCTCGCCGCCGCCTTCGCGGCCGACTGGGCTTTCGTGGACGGCGAGATCCTCGACGGCCCCCCGTGGTTCGTCGACC
TCGAGCCGTGCGGACCGAGCGTCGCCCGGGCCGTCACATCCGGCCCGGACGCGGACGTCGAGAAGATCGAGCAGGTCATC
CTCCAGGCCCTCGCCTGCGCGCGCCGGTCGGTGCGCTTCGTGACGCCCTACTTCCTGCCCGACGAGCTGGTGACCGGCGC
GCTCGCCCAGGCCGCGACCCGGGGCATCACCGTCGACGTCATCATCCCGCGGGTCAGCGACCACCCGTACATCGACTGGG
CGACCCGGGCGCATCTCGATCCGCTGTTGCGGGCCGGCGTGAACGTCTGGCTCGACGAGCCGCCCTTCGACCATTCCAAG
GCGATGGTGGTCGACGACATCTGGTGCTTCGTCGGCAGCGCCAACTGGGACATGCGCAGCTTCCGGCTCAATTTCGAGCT
GAACGTCGAGATCATCGACGCGGACCTCGCGGCCCACCTCGACCGGTTCATGCGCGGCAAGATGGAGGCGCGGCTCAGCC
GGGAGGATCTCGCCGCGCGCGGCCTGCCGCTCCGTCTGCGCGACGCAGGCGTGCGACTGCTGCTGCCATACCTTTGA

Upstream 100 bases:

>100_bases
CGGCCATTGCGCATCCGGCCAAGCCGGCGGATTGATCCGCCCGCCGCATGGGCTTTAGGTCGATCCGGCGCCCCTCGACG
CGAACCCGGATAGCCGCACC

Downstream 100 bases:

>100_bases
ACCGGGCGGGCGCGTGCGCCTCCGGCGCCGGGCCGAGATGGACGACGATCGGCCGGTGATCGGAGCGCCCCCGCGGCAGC
ACCGCGTGCGCGTGGCAGAC

Product: phospholipase D/transphosphatidylase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MENAITRWVQSTALMRSDVEALLGFALAIGVTLHALLRKRRVSVAVGWIGLAWLSPIFGTALYLTFGINRVSRRARRLRT
KPSDATQLPDTDDAVVPEALWPLDRAIRRITGLPAFAGNTVQMFRNGDAAYPVMLAAIREARTSIGLSSYIFRDDATGRE
FCDALVEAQARGVKVRVIIDGIGGGYFRAPVYRRLTAAAVPAALFMHSALPWRMPFLNLRSHKKLLVIDGRIAFTGGLNI
SHPNRVALKPEHPIRDTHFRMTGPVVEQLAAAFAADWAFVDGEILDGPPWFVDLEPCGPSVARAVTSGPDADVEKIEQVI
LQALACARRSVRFVTPYFLPDELVTGALAQAATRGITVDVIIPRVSDHPYIDWATRAHLDPLLRAGVNVWLDEPPFDHSK
AMVVDDIWCFVGSANWDMRSFRLNFELNVEIIDADLAAHLDRFMRGKMEARLSREDLAARGLPLRLRDAGVRLLLPYL

Sequences:

>Translated_478_residues
MENAITRWVQSTALMRSDVEALLGFALAIGVTLHALLRKRRVSVAVGWIGLAWLSPIFGTALYLTFGINRVSRRARRLRT
KPSDATQLPDTDDAVVPEALWPLDRAIRRITGLPAFAGNTVQMFRNGDAAYPVMLAAIREARTSIGLSSYIFRDDATGRE
FCDALVEAQARGVKVRVIIDGIGGGYFRAPVYRRLTAAAVPAALFMHSALPWRMPFLNLRSHKKLLVIDGRIAFTGGLNI
SHPNRVALKPEHPIRDTHFRMTGPVVEQLAAAFAADWAFVDGEILDGPPWFVDLEPCGPSVARAVTSGPDADVEKIEQVI
LQALACARRSVRFVTPYFLPDELVTGALAQAATRGITVDVIIPRVSDHPYIDWATRAHLDPLLRAGVNVWLDEPPFDHSK
AMVVDDIWCFVGSANWDMRSFRLNFELNVEIIDADLAAHLDRFMRGKMEARLSREDLAARGLPLRLRDAGVRLLLPYL
>Mature_478_residues
MENAITRWVQSTALMRSDVEALLGFALAIGVTLHALLRKRRVSVAVGWIGLAWLSPIFGTALYLTFGINRVSRRARRLRT
KPSDATQLPDTDDAVVPEALWPLDRAIRRITGLPAFAGNTVQMFRNGDAAYPVMLAAIREARTSIGLSSYIFRDDATGRE
FCDALVEAQARGVKVRVIIDGIGGGYFRAPVYRRLTAAAVPAALFMHSALPWRMPFLNLRSHKKLLVIDGRIAFTGGLNI
SHPNRVALKPEHPIRDTHFRMTGPVVEQLAAAFAADWAFVDGEILDGPPWFVDLEPCGPSVARAVTSGPDADVEKIEQVI
LQALACARRSVRFVTPYFLPDELVTGALAQAATRGITVDVIIPRVSDHPYIDWATRAHLDPLLRAGVNVWLDEPPFDHSK
AMVVDDIWCFVGSANWDMRSFRLNFELNVEIIDADLAAHLDRFMRGKMEARLSREDLAARGLPLRLRDAGVRLLLPYL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=449, Percent_Identity=30.9576837416481, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1787007, Length=352, Percent_Identity=31.8181818181818, Blast_Score=135, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 52981; Mature: 52981

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENAITRWVQSTALMRSDVEALLGFALAIGVTLHALLRKRRVSVAVGWIGLAWLSPIFGT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHH
ALYLTFGINRVSRRARRLRTKPSDATQLPDTDDAVVPEALWPLDRAIRRITGLPAFAGNT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
VQMFRNGDAAYPVMLAAIREARTSIGLSSYIFRDDATGREFCDALVEAQARGVKVRVIID
EEEEECCCCHHHHHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHHHHHCCEEEEEEEE
GIGGGYFRAPVYRRLTAAAVPAALFMHSALPWRMPFLNLRSHKKLLVIDGRIAFTGGLNI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECEEEEECCCCC
SHPNRVALKPEHPIRDTHFRMTGPVVEQLAAAFAADWAFVDGEILDGPPWFVDLEPCGPS
CCCCEEEECCCCCCCCCCEEECCHHHHHHHHHHHHCHHEECCEEECCCCCEEEECCCCHH
VARAVTSGPDADVEKIEQVILQALACARRSVRFVTPYFLPDELVTGALAQAATRGITVDV
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCEEEE
IIPRVSDHPYIDWATRAHLDPLLRAGVNVWLDEPPFDHSKAMVVDDIWCFVGSANWDMRS
EEECCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEHEEHEECCCCCCEEE
FRLNFELNVEIIDADLAAHLDRFMRGKMEARLSREDLAARGLPLRLRDAGVRLLLPYL
EEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEEEECCCCEEEECCC
>Mature Secondary Structure
MENAITRWVQSTALMRSDVEALLGFALAIGVTLHALLRKRRVSVAVGWIGLAWLSPIFGT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHH
ALYLTFGINRVSRRARRLRTKPSDATQLPDTDDAVVPEALWPLDRAIRRITGLPAFAGNT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
VQMFRNGDAAYPVMLAAIREARTSIGLSSYIFRDDATGREFCDALVEAQARGVKVRVIID
EEEEECCCCHHHHHHHHHHHHHHHCCHHHEEEECCCCHHHHHHHHHHHHHCCEEEEEEEE
GIGGGYFRAPVYRRLTAAAVPAALFMHSALPWRMPFLNLRSHKKLLVIDGRIAFTGGLNI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECEEEEECCCCC
SHPNRVALKPEHPIRDTHFRMTGPVVEQLAAAFAADWAFVDGEILDGPPWFVDLEPCGPS
CCCCEEEECCCCCCCCCCEEECCHHHHHHHHHHHHCHHEECCEEECCCCCEEEECCCCHH
VARAVTSGPDADVEKIEQVILQALACARRSVRFVTPYFLPDELVTGALAQAATRGITVDV
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCEEEE
IIPRVSDHPYIDWATRAHLDPLLRAGVNVWLDEPPFDHSKAMVVDDIWCFVGSANWDMRS
EEECCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEHEEHEECCCCCCEEE
FRLNFELNVEIIDADLAAHLDRFMRGKMEARLSREDLAARGLPLRLRDAGVRLLLPYL
EEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCEEEECCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377 [H]