Click here to switch to the map view.

The map label for this gene is yafC [H]

Identifier: 170746996

GI number: 170746996

Start: 605231

End: 606121

Strand: Reverse

Name: yafC [H]

Synonym: Mrad2831_0562

Alternate gene names: 170746996

Gene position: 606121-605231 (Counterclockwise)

Preceding gene: 170746997

Following gene: 170746995

Centisome position: 9.97

GC content: 68.35

Gene sequence:

>891_bases
TTGGACTGGGACAAGATCCGGATCTTCCTCAACGTCGCGGAGGCGGGGAGCTTCACCAAGGCGGGTGACGATATCGGGCT
CAGCCAATCGGCGGTGAGCCGCCAGATCAGCGCCCTGGAGCGCGAGCTGAAGGCGCCGCTGTTCCACCGCCATGCCCGCG
GCCTGCTGCTGACCGAGCAGGGCGACCTGCTGTTCCGCGCCGCGCGAGATATGAAGCTGCGCCTGGAGAATACCCGGGCC
CGGCTCGTGGAGACCTCCGAGCGGCCGACCGGCGATCTCAAGGTGACCACCACGGTCGGCCTCGGCACGTCCTGGCTGTC
GCAGCGTGTGGGCGAGTTCCTCGACCTCTACCCGGACGTGCGCATCGAGCTGATCCTGACCAACGAGGAGCTCGATCTGG
CCATGCGCGAGGCCGACGTGGCGATTCGCATGCGGCGCCCGGCGCAGCCGGACCTGATCCAGCGGCGGCTGTTCACCGTG
CACTACCACGCCTTCGCGAGCCCGGATTACGTCAAGCGCTTCGGCGAGCCCAAGACCATCGAGGAGCTCGACGACCACCG
CCTCGTCTCCTTCGGCGGCAACGAGCCCTCGTACCTGCTGGCCGCCCACTGGCTCGCCGATGCCGGCCGCGAGGGTCGCG
AGCGTCGCCAGGTTCACCTGACGGTGAACAATATCGGCGCCCTGCAACGCGCGATGGAGGCCGGCGCCGGCATCGGCATC
CTGCCCGACTACGCGGTGGATGGCGGGCAGCAACTCGTGCAGGTGTTGCGCGAGACCGAGATGCCGAGCCTCGAGAGTTA
CCTCGTCTACGCCGAGGAGATGCGCTCGGTCGCCCGCGTGCAGGCGTTCCGCGACTTCCTCGTCTCGAAGGCGCAGCGCT
GGACCTACTGA

Upstream 100 bases:

>100_bases
CGCGCGGCACCGCGCAGTTCGCCCGGTGATCTGCCCGCCGCTTCGCGCCCACTCCACCCCGGGCGCGTCCCGCGGGCGCG
CGCTGGTAGGGGTTCTCGCC

Downstream 100 bases:

>100_bases
GCATCCGATGGTTGACTCCGCCTCCTCACGGAGACGATTCGATGCGCGCTGCTCTCTACGCTCTGATCCTCGTCGCGGGA
CCGACCGGCGTGATGGCGGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDWDKIRIFLNVAEAGSFTKAGDDIGLSQSAVSRQISALERELKAPLFHRHARGLLLTEQGDLLFRAARDMKLRLENTRA
RLVETSERPTGDLKVTTTVGLGTSWLSQRVGEFLDLYPDVRIELILTNEELDLAMREADVAIRMRRPAQPDLIQRRLFTV
HYHAFASPDYVKRFGEPKTIEELDDHRLVSFGGNEPSYLLAAHWLADAGREGRERRQVHLTVNNIGALQRAMEAGAGIGI
LPDYAVDGGQQLVQVLRETEMPSLESYLVYAEEMRSVARVQAFRDFLVSKAQRWTY

Sequences:

>Translated_296_residues
MDWDKIRIFLNVAEAGSFTKAGDDIGLSQSAVSRQISALERELKAPLFHRHARGLLLTEQGDLLFRAARDMKLRLENTRA
RLVETSERPTGDLKVTTTVGLGTSWLSQRVGEFLDLYPDVRIELILTNEELDLAMREADVAIRMRRPAQPDLIQRRLFTV
HYHAFASPDYVKRFGEPKTIEELDDHRLVSFGGNEPSYLLAAHWLADAGREGRERRQVHLTVNNIGALQRAMEAGAGIGI
LPDYAVDGGQQLVQVLRETEMPSLESYLVYAEEMRSVARVQAFRDFLVSKAQRWTY
>Mature_296_residues
MDWDKIRIFLNVAEAGSFTKAGDDIGLSQSAVSRQISALERELKAPLFHRHARGLLLTEQGDLLFRAARDMKLRLENTRA
RLVETSERPTGDLKVTTTVGLGTSWLSQRVGEFLDLYPDVRIELILTNEELDLAMREADVAIRMRRPAQPDLIQRRLFTV
HYHAFASPDYVKRFGEPKTIEELDDHRLVSFGGNEPSYLLAAHWLADAGREGRERRQVHLTVNNIGALQRAMEAGAGIGI
LPDYAVDGGQQLVQVLRETEMPSLESYLVYAEEMRSVARVQAFRDFLVSKAQRWTY

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=30.7167235494881, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI145693193, Length=305, Percent_Identity=28.8524590163934, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787128, Length=305, Percent_Identity=27.2131147540984, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1789440, Length=298, Percent_Identity=25.503355704698, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789639, Length=289, Percent_Identity=26.2975778546713, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787589, Length=284, Percent_Identity=27.8169014084507, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=24.9134948096886, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789173, Length=303, Percent_Identity=25.7425742574257, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788706, Length=290, Percent_Identity=23.1034482758621, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI157672245, Length=226, Percent_Identity=26.5486725663717, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87082132, Length=260, Percent_Identity=27.6923076923077, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788626, Length=189, Percent_Identity=29.6296296296296, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI145693105, Length=244, Percent_Identity=22.5409836065574, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33593; Mature: 33593

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWDKIRIFLNVAEAGSFTKAGDDIGLSQSAVSRQISALERELKAPLFHRHARGLLLTEQ
CCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCEEEECC
GDLLFRAARDMKLRLENTRARLVETSERPTGDLKVTTTVGLGTSWLSQRVGEFLDLYPDV
CCCHHHHHHHHHEEEHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCC
RIELILTNEELDLAMREADVAIRMRRPAQPDLIQRRLFTVHYHAFASPDYVKRFGEPKTI
EEEEEEECCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHEEHHCCCCHHHHHCCCCCCH
EELDDHRLVSFGGNEPSYLLAAHWLADAGREGRERRQVHLTVNNIGALQRAMEAGAGIGI
HHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCC
LPDYAVDGGQQLVQVLRETEMPSLESYLVYAEEMRSVARVQAFRDFLVSKAQRWTY
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDWDKIRIFLNVAEAGSFTKAGDDIGLSQSAVSRQISALERELKAPLFHRHARGLLLTEQ
CCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCEEEECC
GDLLFRAARDMKLRLENTRARLVETSERPTGDLKVTTTVGLGTSWLSQRVGEFLDLYPDV
CCCHHHHHHHHHEEEHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCC
RIELILTNEELDLAMREADVAIRMRRPAQPDLIQRRLFTVHYHAFASPDYVKRFGEPKTI
EEEEEEECCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHEEHHCCCCHHHHHCCCCCCH
EELDDHRLVSFGGNEPSYLLAAHWLADAGREGRERRQVHLTVNNIGALQRAMEAGAGIGI
HHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCC
LPDYAVDGGQQLVQVLRETEMPSLESYLVYAEEMRSVARVQAFRDFLVSKAQRWTY
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]