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The map label for this gene is aes [H]

Identifier: 170746974

GI number: 170746974

Start: 580083

End: 581039

Strand: Reverse

Name: aes [H]

Synonym: Mrad2831_0540

Alternate gene names: 170746974

Gene position: 581039-580083 (Counterclockwise)

Preceding gene: 170746977

Following gene: 170746973

Centisome position: 9.56

GC content: 72.1

Gene sequence:

>957_bases
ATGATCGACCACGCCCTGTTCGACCCGGCGACGATCGATCCCGAGACGCTCCGGCTCAATGCCGAGATCGTCGCGGCGCA
CGCCGCGCTGCCCGATCCCTGGTCGCTGCCGATCGCCGAGGTTCGCGAGCGCCGCCGTCTGGGCACCAAGGCGGCCCCCG
CCATGCCGCACAGCGCCCGGGCGGAGTGGCTGACGATCGACGGGCCGGGCGGTCCGCTCAAGCTCCGCGTGGTCCGGCCC
AAGCCCGGCGTGAAGCTGCGCGGCGCCTACCTGCACATTCACCGCGGCGGCTGGGTCTGGGGCTCCGCCGACGAGCAGGA
TCCCTGGCTGGAGCGCATCGCCGACACCTGCGGCTTCGTCGCCCTGTCGGTCGAGTATCGCCTCGCCCCCGAGCATCCCT
ATCCGGCGGCCCTGCTCGACTGCGAGGCCGCGGCCCTGTGGCTGGCCGGTCCGGGGAAGACCGAGTTCGGCATCCACGCG
CTGACCATCGGCGGCGAGTCGGTGGGCGCGCACCTCGCCGTGATGGTGCTGCTGCGGCTGCGCGACAAACACAACCTCCC
GCGGGCCTTCCGGGGCGCCAACCTCAACGCCGGCTTCTTCGATCTCGGCCTCACCCCGTCGGTGCGGAACTGGGGCGAGG
AGCGGCTGGTCGTGAACACCCGCGACCTGACCAAGTTCGCCGACGGCTACGTCCGCGAGGGCATCGACCGGCGCCGGCCG
GACGTGTCGCCGATCTACGCCGACCTGAAGGGCCTGCCGCCCGCCCTGTTCACCATCGGCACCGCCGATCCCCTCCTCGA
CGACACGCTCTACATGTCGGCGCGCTGGGCCGCGGCCGGCAATGGCGGACACACGGCGATCTATCCCGGCGGCTGCCATG
TCTTCGCCCGCTACCCGAGCCCGATGAGCGAGCGGGCGCTGGAGCTGATCGACCGCTTCCTGATGGCGCTCGCCTGA

Upstream 100 bases:

>100_bases
CGACGCGCGACTCGCGCGGCGCGGCCTCGACCAAAGGGAGAACTCCCGGGAACCGGGATTGGTGTGAGAGGCTCTGAGTG
CCGACGGGACCATACGCACG

Downstream 100 bases:

>100_bases
GCGTCCCGTCCGAGATCCTGCCGGAAGGATTCCGCGCCTGGTTCGCGGGCCGCGGCTGGGCGCCCCGGTCGCACCAGCTC
GATCTCCTGCGGATCGCCGG

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MIDHALFDPATIDPETLRLNAEIVAAHAALPDPWSLPIAEVRERRRLGTKAAPAMPHSARAEWLTIDGPGGPLKLRVVRP
KPGVKLRGAYLHIHRGGWVWGSADEQDPWLERIADTCGFVALSVEYRLAPEHPYPAALLDCEAAALWLAGPGKTEFGIHA
LTIGGESVGAHLAVMVLLRLRDKHNLPRAFRGANLNAGFFDLGLTPSVRNWGEERLVVNTRDLTKFADGYVREGIDRRRP
DVSPIYADLKGLPPALFTIGTADPLLDDTLYMSARWAAAGNGGHTAIYPGGCHVFARYPSPMSERALELIDRFLMALA

Sequences:

>Translated_318_residues
MIDHALFDPATIDPETLRLNAEIVAAHAALPDPWSLPIAEVRERRRLGTKAAPAMPHSARAEWLTIDGPGGPLKLRVVRP
KPGVKLRGAYLHIHRGGWVWGSADEQDPWLERIADTCGFVALSVEYRLAPEHPYPAALLDCEAAALWLAGPGKTEFGIHA
LTIGGESVGAHLAVMVLLRLRDKHNLPRAFRGANLNAGFFDLGLTPSVRNWGEERLVVNTRDLTKFADGYVREGIDRRRP
DVSPIYADLKGLPPALFTIGTADPLLDDTLYMSARWAAAGNGGHTAIYPGGCHVFARYPSPMSERALELIDRFLMALA
>Mature_318_residues
MIDHALFDPATIDPETLRLNAEIVAAHAALPDPWSLPIAEVRERRRLGTKAAPAMPHSARAEWLTIDGPGGPLKLRVVRP
KPGVKLRGAYLHIHRGGWVWGSADEQDPWLERIADTCGFVALSVEYRLAPEHPYPAALLDCEAAALWLAGPGKTEFGIHA
LTIGGESVGAHLAVMVLLRLRDKHNLPRAFRGANLNAGFFDLGLTPSVRNWGEERLVVNTRDLTKFADGYVREGIDRRRP
DVSPIYADLKGLPPALFTIGTADPLLDDTLYMSARWAAAGNGGHTAIYPGGCHVFARYPSPMSERALELIDRFLMALA

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=280, Percent_Identity=27.1428571428571, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540028, Length=117, Percent_Identity=34.1880341880342, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17567059, Length=102, Percent_Identity=37.2549019607843, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71996133, Length=122, Percent_Identity=32.7868852459016, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34761; Mature: 34761

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDHALFDPATIDPETLRLNAEIVAAHAALPDPWSLPIAEVRERRRLGTKAAPAMPHSAR
CCCCCCCCCCCCCHHHEEECCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
AEWLTIDGPGGPLKLRVVRPKPGVKLRGAYLHIHRGGWVWGSADEQDPWLERIADTCGFV
CCEEEEECCCCCEEEEEECCCCCCEEEEEEEEEECCCEEECCCCCCCHHHHHHHHHHCEE
ALSVEYRLAPEHPYPAALLDCEAAALWLAGPGKTEFGIHALTIGGESVGAHLAVMVLLRL
EEEEEEEECCCCCCCHHEEECCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
RDKHNLPRAFRGANLNAGFFDLGLTPSVRNWGEERLVVNTRDLTKFADGYVREGIDRRRP
HHCCCCCHHHCCCCCCCCEEECCCCCCHHCCCCCEEEEECHHHHHHHHHHHHHHHHHCCC
DVSPIYADLKGLPPALFTIGTADPLLDDTLYMSARWAAAGNGGHTAIYPGGCHVFARYPS
CCCHHHHHHCCCCHHEEEECCCCCHHCCHHEEEEEEEECCCCCCEEEECCCEEEEEECCC
PMSERALELIDRFLMALA
CHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDHALFDPATIDPETLRLNAEIVAAHAALPDPWSLPIAEVRERRRLGTKAAPAMPHSAR
CCCCCCCCCCCCCHHHEEECCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
AEWLTIDGPGGPLKLRVVRPKPGVKLRGAYLHIHRGGWVWGSADEQDPWLERIADTCGFV
CCEEEEECCCCCEEEEEECCCCCCEEEEEEEEEECCCEEECCCCCCCHHHHHHHHHHCEE
ALSVEYRLAPEHPYPAALLDCEAAALWLAGPGKTEFGIHALTIGGESVGAHLAVMVLLRL
EEEEEEEECCCCCCCHHEEECCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
RDKHNLPRAFRGANLNAGFFDLGLTPSVRNWGEERLVVNTRDLTKFADGYVREGIDRRRP
HHCCCCCHHHCCCCCCCCEEECCCCCCHHCCCCCEEEEECHHHHHHHHHHHHHHHHHCCC
DVSPIYADLKGLPPALFTIGTADPLLDDTLYMSARWAAAGNGGHTAIYPGGCHVFARYPS
CCCHHHHHHCCCCHHEEEECCCCCHHCCHHEEEEEEEECCCCCCEEEECCCEEEEEECCC
PMSERALELIDRFLMALA
CHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA