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The map label for this gene is aspS [H]

Identifier: 170746938

GI number: 170746938

Start: 541796

End: 543673

Strand: Reverse

Name: aspS [H]

Synonym: Mrad2831_0504

Alternate gene names: 170746938

Gene position: 543673-541796 (Counterclockwise)

Preceding gene: 170746945

Following gene: 170746937

Centisome position: 8.95

GC content: 68.26

Gene sequence:

>1878_bases
ATGGTGCCGGCCTTCCCACAGAAGAAGCCCGTCATGCACCGTTACCGTTCCCACACCTGCGGGGCGCTCCGCCCGTCCGA
CGTCGGGCAGAGTGTCCGCCTCTCCGGCTGGTGCCACCGCATCCGCGACCACGGGGGCGTGCTGTTCGTGGATCTGCGGG
ACCATTACGGCCTGACGCAGTGCGTGATCGATTCCGATTCCCCGGCCTTCAAGGCCGCCGAGACCGTCCGCTCGGAATGG
GTCGTGCGGATCGACGGCCGCGTCCGCACGCGACCGGCCGGTACCGAGAATGCCGAGCTGCCGACCGGCGCGGTCGAGGT
CTACATCGACGACCTCGAGGTGCTGGGTCCGGCCGGCGAGCTGCCGCTGCCGGTCTTCGGCGACCTCGAGTACCCGGAGG
AGACGCGGCTCCGGTACCGCTTCCTCGACCTGCGCCGGGAGAAGCTGCACGCCAACATCATGAAGCGCGGCGCGATCATC
GACTCGCTCCGCCGCCGGATGCGCGAGGGCGGCTTCTTCGAGTTCCAGACGCCGATCCTGACCGCGTCGAGCCCGGAAGG
CGCGCGCGACTACCTCGTGCCGTCGCGGGTCCATCCCGGCAAGTTCTACGCGCTCCCGCAGGCGCCGCAGCAGTTCAAGC
AGCTGACGATGATCGCGGGCTTCGACCGCTACTTCCAGATCGCGCCCTGCTTCCGCGACGAGGATGCCCGCGCCGACCGC
TCGCCGGGCGAGTTCTACCAGCTCGACATCGAGATGAGCTTCGTCACCCAGGAGGACGTGTTCCAGGCGGTCGAGCCGGT
CCTGCGCGGCGTGTTCGAGGAGTTCGCCGAGGGCAAGCGCGTCACCAAGGAATTCCCGCGGATCACCTACGCGGACTCGA
TGCTGAAGTACGGCGTCGACAAGCCGGACCTGCGCAATCCGCTGATCATCGCCGACGTCACCGACCTGTTCGCCCGCGAG
GACGTGGCCTTCAAGGCGTTCAAGGGCGTGATCGCTTCCGGCGGTGTGGTCCGGGCGATCCCGGCGACCGGTGCGGCGGC
GCAGTCGCGCTCGTTCTTCGACAAGCTCAACGACTGGGCCCGTTCGGAGGGCGCGCCGGGGCTCGGCTATGTCGTGTTCG
AGGAGGAGAACGGGCAGCTCACCGGCAAGGGGCCGATCGCCAAGTTCATCCCGGCCGAGGTCCAGGCGCTGATCGCCGAG
CGGGCCGGTGCCAAGGCGGGCGACGCGGTGTTCTTCTCGGCCGGCGCCGAGGGCAAGGCGGCCGGCCTCGCCGGCAAGGC
GCGCATCCGCATCGGCGACGAGCTAGGCCTCTGCGACAAGGACCAGTACGCCTTCTGCTGGATCACCGACTTCCCGATGT
ACGAGTGGAGCGAGGAAGAGAAGCGGATCGACTTCTCCCACAACCCGTTCTCGATGCCGAACATCGACCGCGAGGCGTTC
CTGGCGCTCGACCTCGACGCGCTCTCCAGCGAGGACGAGAACGGCGCGAACACCAAGCGGATCCTGGACATCAAGGCGTT
CCAGTACGACATCGTCTGCAACGGCGTGGAGCTGTCGTCGGGCGCGATCCGCAACCACCGTCCGGACGTGATGGAGAAGG
CCTTCGCGATCGCCGGCTACGGCCACGAGGTCCTGGAGCAGAAGTTCGGCGGCATGCTGAACGCCCTGCGCATGGGCGCT
CCGCCGCACGGCGGCATCGCGCCGGGTGTGGACCGCATCGTCATGCTCCTGTGCAACGAGCCGAACATCCGCGAGGTGGT
GCTGTTCCCGATGAACCAGCGCGCCGAGGACCTGATGATGGGCGCGCCCTCCGAGGCGACGCCCAAGCAGCTGCGCGAGC
TGCACATCCGGCTCAACCTGCCCGAGAAGAAGGCCTGA

Upstream 100 bases:

>100_bases
GGGGATCGGGAGCCTCTTCCGGTAGGCCGGCGGGCGAGTCGCGCGCGGCTTTCGGCGCCGTTCTGCGCGCCCCGCGCTTG
ACTTGGGGGGCCTCGCGTGA

Downstream 100 bases:

>100_bases
GGCGGCGCGAACCGCCGCCCGGTGCCGAGTCTCGTCGCGATCGTCGTCGCGCATGACAGCGCCGACGTGCTGCCCGGCTG
CCTCGCGGCGCTGGCCGGGC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MVPAFPQKKPVMHRYRSHTCGALRPSDVGQSVRLSGWCHRIRDHGGVLFVDLRDHYGLTQCVIDSDSPAFKAAETVRSEW
VVRIDGRVRTRPAGTENAELPTGAVEVYIDDLEVLGPAGELPLPVFGDLEYPEETRLRYRFLDLRREKLHANIMKRGAII
DSLRRRMREGGFFEFQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQQFKQLTMIAGFDRYFQIAPCFRDEDARADR
SPGEFYQLDIEMSFVTQEDVFQAVEPVLRGVFEEFAEGKRVTKEFPRITYADSMLKYGVDKPDLRNPLIIADVTDLFARE
DVAFKAFKGVIASGGVVRAIPATGAAAQSRSFFDKLNDWARSEGAPGLGYVVFEEENGQLTGKGPIAKFIPAEVQALIAE
RAGAKAGDAVFFSAGAEGKAAGLAGKARIRIGDELGLCDKDQYAFCWITDFPMYEWSEEEKRIDFSHNPFSMPNIDREAF
LALDLDALSSEDENGANTKRILDIKAFQYDIVCNGVELSSGAIRNHRPDVMEKAFAIAGYGHEVLEQKFGGMLNALRMGA
PPHGGIAPGVDRIVMLLCNEPNIREVVLFPMNQRAEDLMMGAPSEATPKQLRELHIRLNLPEKKA

Sequences:

>Translated_625_residues
MVPAFPQKKPVMHRYRSHTCGALRPSDVGQSVRLSGWCHRIRDHGGVLFVDLRDHYGLTQCVIDSDSPAFKAAETVRSEW
VVRIDGRVRTRPAGTENAELPTGAVEVYIDDLEVLGPAGELPLPVFGDLEYPEETRLRYRFLDLRREKLHANIMKRGAII
DSLRRRMREGGFFEFQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQQFKQLTMIAGFDRYFQIAPCFRDEDARADR
SPGEFYQLDIEMSFVTQEDVFQAVEPVLRGVFEEFAEGKRVTKEFPRITYADSMLKYGVDKPDLRNPLIIADVTDLFARE
DVAFKAFKGVIASGGVVRAIPATGAAAQSRSFFDKLNDWARSEGAPGLGYVVFEEENGQLTGKGPIAKFIPAEVQALIAE
RAGAKAGDAVFFSAGAEGKAAGLAGKARIRIGDELGLCDKDQYAFCWITDFPMYEWSEEEKRIDFSHNPFSMPNIDREAF
LALDLDALSSEDENGANTKRILDIKAFQYDIVCNGVELSSGAIRNHRPDVMEKAFAIAGYGHEVLEQKFGGMLNALRMGA
PPHGGIAPGVDRIVMLLCNEPNIREVVLFPMNQRAEDLMMGAPSEATPKQLRELHIRLNLPEKKA
>Mature_625_residues
MVPAFPQKKPVMHRYRSHTCGALRPSDVGQSVRLSGWCHRIRDHGGVLFVDLRDHYGLTQCVIDSDSPAFKAAETVRSEW
VVRIDGRVRTRPAGTENAELPTGAVEVYIDDLEVLGPAGELPLPVFGDLEYPEETRLRYRFLDLRREKLHANIMKRGAII
DSLRRRMREGGFFEFQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQQFKQLTMIAGFDRYFQIAPCFRDEDARADR
SPGEFYQLDIEMSFVTQEDVFQAVEPVLRGVFEEFAEGKRVTKEFPRITYADSMLKYGVDKPDLRNPLIIADVTDLFARE
DVAFKAFKGVIASGGVVRAIPATGAAAQSRSFFDKLNDWARSEGAPGLGYVVFEEENGQLTGKGPIAKFIPAEVQALIAE
RAGAKAGDAVFFSAGAEGKAAGLAGKARIRIGDELGLCDKDQYAFCWITDFPMYEWSEEEKRIDFSHNPFSMPNIDREAF
LALDLDALSSEDENGANTKRILDIKAFQYDIVCNGVELSSGAIRNHRPDVMEKAFAIAGYGHEVLEQKFGGMLNALRMGA
PPHGGIAPGVDRIVMLLCNEPNIREVVLFPMNQRAEDLMMGAPSEATPKQLRELHIRLNLPEKKA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=617, Percent_Identity=35.1701782820097, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI45439306, Length=305, Percent_Identity=27.2131147540984, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI226491502, Length=276, Percent_Identity=24.6376811594203, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI4758762, Length=335, Percent_Identity=22.9850746268657, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1788173, Length=608, Percent_Identity=41.6118421052632, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1790571, Length=290, Percent_Identity=27.2413793103448, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789256, Length=220, Percent_Identity=28.1818181818182, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI32566633, Length=620, Percent_Identity=34.3548387096774, Blast_Score=307, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI71984122, Length=297, Percent_Identity=24.9158249158249, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17551876, Length=141, Percent_Identity=30.4964539007092, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=658, Percent_Identity=29.483282674772, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321807, Length=245, Percent_Identity=29.3877551020408, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6323011, Length=287, Percent_Identity=25.7839721254355, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24584738, Length=620, Percent_Identity=33.7096774193548, Blast_Score=318, Evalue=5e-87,
Organism=Drosophila melanogaster, GI17136276, Length=262, Percent_Identity=25.1908396946565, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19921528, Length=303, Percent_Identity=24.7524752475248, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 69626; Mature: 69626

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPAFPQKKPVMHRYRSHTCGALRPSDVGQSVRLSGWCHRIRDHGGVLFVDLRDHYGLTQ
CCCCCCCCCHHHHHHHCCCCCCCCHHHCCCCEEHHHHHHHHHCCCCEEEEEECCCCCCEE
CVIDSDSPAFKAAETVRSEWVVRIDGRVRTRPAGTENAELPTGAVEVYIDDLEVLGPAGE
EEECCCCCHHHHHHHHHHCEEEEECCEEEECCCCCCCCCCCCCEEEEEEECHHHCCCCCC
LPLPVFGDLEYPEETRLRYRFLDLRREKLHANIMKRGAIIDSLRRRMREGGFFEFQTPIL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEE
TASSPEGARDYLVPSRVHPGKFYALPQAPQQFKQLTMIAGFDRYFQIAPCFRDEDARADR
ECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCC
SPGEFYQLDIEMSFVTQEDVFQAVEPVLRGVFEEFAEGKRVTKEFPRITYADSMLKYGVD
CCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCCC
KPDLRNPLIIADVTDLFAREDVAFKAFKGVIASGGVVRAIPATGAAAQSRSFFDKLNDWA
CCCCCCCEEEEEHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHH
RSEGAPGLGYVVFEEENGQLTGKGPIAKFIPAEVQALIAERAGAKAGDAVFFSAGAEGKA
HCCCCCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
AGLAGKARIRIGDELGLCDKDQYAFCWITDFPMYEWSEEEKRIDFSHNPFSMPNIDREAF
CCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCHHCCCCCCCCCCCCCCCCCCEE
LALDLDALSSEDENGANTKRILDIKAFQYDIVCNGVELSSGAIRNHRPDVMEKAFAIAGY
EEEEHHHHCCCCCCCCCCEEEEEEEEEEEEEEECCEEECCCCCCCCCCHHHHHHHHHCCC
GHEVLEQKFGGMLNALRMGAPPHGGIAPGVDRIVMLLCNEPNIREVVLFPMNQRAEDLMM
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEEECCCCCEEEEEECCCCCHHHHHC
GAPSEATPKQLRELHIRLNLPEKKA
CCCCCCCHHHHHEEEEEECCCCCCC
>Mature Secondary Structure
MVPAFPQKKPVMHRYRSHTCGALRPSDVGQSVRLSGWCHRIRDHGGVLFVDLRDHYGLTQ
CCCCCCCCCHHHHHHHCCCCCCCCHHHCCCCEEHHHHHHHHHCCCCEEEEEECCCCCCEE
CVIDSDSPAFKAAETVRSEWVVRIDGRVRTRPAGTENAELPTGAVEVYIDDLEVLGPAGE
EEECCCCCHHHHHHHHHHCEEEEECCEEEECCCCCCCCCCCCCEEEEEEECHHHCCCCCC
LPLPVFGDLEYPEETRLRYRFLDLRREKLHANIMKRGAIIDSLRRRMREGGFFEFQTPIL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEE
TASSPEGARDYLVPSRVHPGKFYALPQAPQQFKQLTMIAGFDRYFQIAPCFRDEDARADR
ECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCC
SPGEFYQLDIEMSFVTQEDVFQAVEPVLRGVFEEFAEGKRVTKEFPRITYADSMLKYGVD
CCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCCC
KPDLRNPLIIADVTDLFAREDVAFKAFKGVIASGGVVRAIPATGAAAQSRSFFDKLNDWA
CCCCCCCEEEEEHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHH
RSEGAPGLGYVVFEEENGQLTGKGPIAKFIPAEVQALIAERAGAKAGDAVFFSAGAEGKA
HCCCCCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
AGLAGKARIRIGDELGLCDKDQYAFCWITDFPMYEWSEEEKRIDFSHNPFSMPNIDREAF
CCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCHHCCCCCCCCCCCCCCCCCCEE
LALDLDALSSEDENGANTKRILDIKAFQYDIVCNGVELSSGAIRNHRPDVMEKAFAIAGY
EEEEHHHHCCCCCCCCCCEEEEEEEEEEEEEEECCEEECCCCCCCCCCHHHHHHHHHCCC
GHEVLEQKFGGMLNALRMGAPPHGGIAPGVDRIVMLLCNEPNIREVVLFPMNQRAEDLMM
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEEECCCCCEEEEEECCCCCHHHHHC
GAPSEATPKQLRELHIRLNLPEKKA
CCCCCCCHHHHHEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA