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The map label for this gene is purN [H]

Identifier: 170746924

GI number: 170746924

Start: 523891

End: 524541

Strand: Reverse

Name: purN [H]

Synonym: Mrad2831_0490

Alternate gene names: 170746924

Gene position: 524541-523891 (Counterclockwise)

Preceding gene: 170746925

Following gene: 170746921

Centisome position: 8.63

GC content: 74.81

Gene sequence:

>651_bases
ATGGCGGCCGCCCGCAAGACCCGCGTCGCCGTCCTGATCTCGGGGCGCGGTTCGAACATGGTCGCGCTGCTCGAGGCCGC
GAAGGATCCCGCCTTCCCGGCCGAGATCGTGCTGGTCCTGTCGAACCGCCCGGCGGCCGCCGGCCTCGCCCGGGCGGCCG
CGGCTGGCATCCCGACGCAGGCGATCGATCACCGGGCCTTCGCCGACCGGGCCGGCTTCGACGCCGCCCTCGACGCGGCG
CTGCGGGCGGCCGAGATCGACCTCGTCTGCCTCGCGGGTTTCATGCGGATCCTCACGACGGAGTTCGTGGCGTCCTGGGC
CGGCCGGATGCTCAACATCCACCCGTCGCTCCTGCCCCTGTTCAAGGGCACGCACACCCACCGGCAGGCGCTGGACGCGG
GCGTGCGCCTGCACGGCTGCACGGTCCACTTCGTCGTGCCGGAGCTCGATGCCGGCCCGATCGTCGCCCAGGCCGCGATC
CCCGTCCGCCAGGACGACGATCCCGACAGCCTCGCCGACCGGGTGATCGTGCAGGAGCGCCGCCTCTACCCGGCGGTGCT
CGCCCTCGTGGCGGGCGGCCGCGCCCGCCTGGAGGGCGAGCGCGTGGTCATCGCCGACGCGGCGCCGGACGGCGCGCTGT
TCAGCGTCTAG

Upstream 100 bases:

>100_bases
ACGAGGCGTTGACCCGGGCCGGCGAGACCCCGGTCCGCCTCGGGCGGATCACGGCGCGCGGGCCCGAGCCGGTCACCTTC
GCCGGCCGCCTCGCCCTCTG

Downstream 100 bases:

>100_bases
CGGGCGGCAGCACGGGCGTTTCCGTCCTGGCGCGCGGGGCGAAGCTATCCGGTAACGCCACGCTCGCCGACGTCTTGCTC
AACGGGTTGCTGCGCTCCGC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MAAARKTRVAVLISGRGSNMVALLEAAKDPAFPAEIVLVLSNRPAAAGLARAAAAGIPTQAIDHRAFADRAGFDAALDAA
LRAAEIDLVCLAGFMRILTTEFVASWAGRMLNIHPSLLPLFKGTHTHRQALDAGVRLHGCTVHFVVPELDAGPIVAQAAI
PVRQDDDPDSLADRVIVQERRLYPAVLALVAGGRARLEGERVVIADAAPDGALFSV

Sequences:

>Translated_216_residues
MAAARKTRVAVLISGRGSNMVALLEAAKDPAFPAEIVLVLSNRPAAAGLARAAAAGIPTQAIDHRAFADRAGFDAALDAA
LRAAEIDLVCLAGFMRILTTEFVASWAGRMLNIHPSLLPLFKGTHTHRQALDAGVRLHGCTVHFVVPELDAGPIVAQAAI
PVRQDDDPDSLADRVIVQERRLYPAVLALVAGGRARLEGERVVIADAAPDGALFSV
>Mature_215_residues
AAARKTRVAVLISGRGSNMVALLEAAKDPAFPAEIVLVLSNRPAAAGLARAAAAGIPTQAIDHRAFADRAGFDAALDAAL
RAAEIDLVCLAGFMRILTTEFVASWAGRMLNIHPSLLPLFKGTHTHRQALDAGVRLHGCTVHFVVPELDAGPIVAQAAIP
VRQDDDPDSLADRVIVQERRLYPAVLALVAGGRARLEGERVVIADAAPDGALFSV

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=193, Percent_Identity=52.3316062176166, Blast_Score=217, Evalue=5e-57,
Organism=Homo sapiens, GI209869995, Length=193, Percent_Identity=52.3316062176166, Blast_Score=217, Evalue=5e-57,
Organism=Homo sapiens, GI209869993, Length=193, Percent_Identity=52.3316062176166, Blast_Score=217, Evalue=5e-57,
Organism=Escherichia coli, GI1788846, Length=202, Percent_Identity=45.5445544554455, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1787483, Length=201, Percent_Identity=30.3482587064677, Blast_Score=91, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=188, Percent_Identity=43.0851063829787, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6320616, Length=174, Percent_Identity=33.9080459770115, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24582400, Length=202, Percent_Identity=50, Blast_Score=201, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22673; Mature: 22542

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAARKTRVAVLISGRGSNMVALLEAAKDPAFPAEIVLVLSNRPAAAGLARAAAAGIPTQ
CCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCHH
AIDHRAFADRAGFDAALDAALRAAEIDLVCLAGFMRILTTEFVASWAGRMLNIHPSLLPL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHH
FKGTHTHRQALDAGVRLHGCTVHFVVPELDAGPIVAQAAIPVRQDDDPDSLADRVIVQER
HCCCCHHHHHHHCCCEEECEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
RLYPAVLALVAGGRARLEGERVVIADAAPDGALFSV
HHHHHHHHHHCCCCCEECCCEEEEEECCCCCCEECC
>Mature Secondary Structure 
AAARKTRVAVLISGRGSNMVALLEAAKDPAFPAEIVLVLSNRPAAAGLARAAAAGIPTQ
CCCCCEEEEEEEECCCCCEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCHH
AIDHRAFADRAGFDAALDAALRAAEIDLVCLAGFMRILTTEFVASWAGRMLNIHPSLLPL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHH
FKGTHTHRQALDAGVRLHGCTVHFVVPELDAGPIVAQAAIPVRQDDDPDSLADRVIVQER
HCCCCHHHHHHHCCCEEECEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
RLYPAVLALVAGGRARLEGERVVIADAAPDGALFSV
HHHHHHHHHHCCCCCEECCCEEEEEECCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]