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The map label for this gene is insK [C]

Identifier: 170746876

GI number: 170746876

Start: 479603

End: 480442

Strand: Reverse

Name: insK [C]

Synonym: Mrad2831_0441

Alternate gene names: 170746876

Gene position: 480442-479603 (Counterclockwise)

Preceding gene: 170746877

Following gene: 170746871

Centisome position: 7.9

GC content: 64.64

Gene sequence:

>840_bases
GTGAGGCCCGCCGTTCGCCGTGAGGTCGCCGGTCACCTGCAGGTGACCTACGGCATCAGCGAGCGTCGGGCCTGTCAGGC
CACCGGTTTCGGCCGCTCGTCTCAGCGTTACCGCAAGCGCGCCGACCCTCAGGTGGCACTGCGCATGCGGCTGAAGGAAC
TCGCTGCCGCACGGGTGCGCTACGGCTACCGGCGGCTGCACATCCTGTTGCGACGGGAGGGCTGGGAGGTGAACCACAAG
CGCACCTACCGGATCTACCGGGACGAAGGGCTGTCGATCCGGCCCAAGCTACCCAAGCGCAAACGGGCCTGGCGCTACCG
ACAAGGGCGGCCCGCGATCGGCGGTCCCAACGAGGTCTGGGCCATGGACTTCATGTCCGATCGCCTGTTCGACGGGCGTC
CATTCCGGATCCTGACGGTCGTCGATTGCCATACGCGAGAGGCGCTCTCGCTCACACCGAGAGCCAATTTCCGCGCCTTC
CAGGTGACCGAGGCCCTGGACGCACTGGTGAAGCTCCGCGGTCGTCCAAAGAGCCTACGGGTTGACAATGGGCCGGAATT
CGCCGGGCGCATGCTCGACCAATGGGCTTATCTGAACGGGGTTGAGATCGACTTCTCCCGACCGGGTAAGCCGACCGACA
ACGCCTACATCGAGTCGTTCAACGGCCGCCTACGGGCAGAGTGCTTGAACGCTTCGTGGTTCCTATCGCTAGCCGATGCC
CGTGAACGCATCGAGGACTGGAGGTGCCACTACAACGAAGATCGACCCCATACAGCCCTGGGCGGCTTGACGCCCCGAGC
CTTCGCCAACCAAGCTGTCACAGCCCGAGAAATTGCATAG

Upstream 100 bases:

>100_bases
GGCTGAAGCAGCTGGAGGACGAGAACAGCAAGCTCAAACGGCTGGTGGCCGACCTGACGCTGGACCGCTCCATGCTGCAG
GATGTGCTCAAGCGAAAGTG

Downstream 100 bases:

>100_bases
GTCGTGGACCACAAACCGGGTCAACTCCAGGCCGACCCTGCACTAATCTTGAGCTCGGACCACCTCGTGGGGGCAGGTCA
TTCCGGGATGCCTAGGGGCT

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MRPAVRREVAGHLQVTYGISERRACQATGFGRSSQRYRKRADPQVALRMRLKELAAARVRYGYRRLHILLRREGWEVNHK
RTYRIYRDEGLSIRPKLPKRKRAWRYRQGRPAIGGPNEVWAMDFMSDRLFDGRPFRILTVVDCHTREALSLTPRANFRAF
QVTEALDALVKLRGRPKSLRVDNGPEFAGRMLDQWAYLNGVEIDFSRPGKPTDNAYIESFNGRLRAECLNASWFLSLADA
RERIEDWRCHYNEDRPHTALGGLTPRAFANQAVTAREIA

Sequences:

>Translated_279_residues
MRPAVRREVAGHLQVTYGISERRACQATGFGRSSQRYRKRADPQVALRMRLKELAAARVRYGYRRLHILLRREGWEVNHK
RTYRIYRDEGLSIRPKLPKRKRAWRYRQGRPAIGGPNEVWAMDFMSDRLFDGRPFRILTVVDCHTREALSLTPRANFRAF
QVTEALDALVKLRGRPKSLRVDNGPEFAGRMLDQWAYLNGVEIDFSRPGKPTDNAYIESFNGRLRAECLNASWFLSLADA
RERIEDWRCHYNEDRPHTALGGLTPRAFANQAVTAREIA
>Mature_279_residues
MRPAVRREVAGHLQVTYGISERRACQATGFGRSSQRYRKRADPQVALRMRLKELAAARVRYGYRRLHILLRREGWEVNHK
RTYRIYRDEGLSIRPKLPKRKRAWRYRQGRPAIGGPNEVWAMDFMSDRLFDGRPFRILTVVDCHTREALSLTPRANFRAF
QVTEALDALVKLRGRPKSLRVDNGPEFAGRMLDQWAYLNGVEIDFSRPGKPTDNAYIESFNGRLRAECLNASWFLSLADA
RERIEDWRCHYNEDRPHTALGGLTPRAFANQAVTAREIA

Specific function: Involved in the transposition of the insertion sequence is150 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=285, Percent_Identity=28.4210526315789, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1786462, Length=167, Percent_Identity=31.1377245508982, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790724, Length=248, Percent_Identity=27.8225806451613, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789424, Length=248, Percent_Identity=27.8225806451613, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789224, Length=248, Percent_Identity=27.8225806451613, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788305, Length=248, Percent_Identity=27.8225806451613, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787669, Length=248, Percent_Identity=27.8225806451613, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786558, Length=248, Percent_Identity=27.8225806451613, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788405, Length=253, Percent_Identity=28.4584980237154, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787263, Length=253, Percent_Identity=28.4584980237154, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786753, Length=253, Percent_Identity=28.4584980237154, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786570, Length=253, Percent_Identity=28.4584980237154, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786490, Length=253, Percent_Identity=28.4584980237154, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32427; Mature: 32427

Theoretical pI: Translated: 11.22; Mature: 11.22

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPAVRREVAGHLQVTYGISERRACQATGFGRSSQRYRKRADPQVALRMRLKELAAARVR
CCCHHHHHHCCCEEEEECCCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHH
YGYRRLHILLRREGWEVNHKRTYRIYRDEGLSIRPKLPKRKRAWRYRQGRPAIGGPNEVW
HHHHHHHEEEECCCCCCCCCEEEEEEECCCCEECCCCCCHHHHHHHCCCCCCCCCCCCEE
AMDFMSDRLFDGRPFRILTVVDCHTREALSLTPRANFRAFQVTEALDALVKLRGRPKSLR
EHHHHHHHCCCCCCEEEEEEEECCCCCCEEECCCCCCEEEHHHHHHHHHHHHCCCCCEEE
VDNGPEFAGRMLDQWAYLNGVEIDFSRPGKPTDNAYIESFNGRLRAECLNASWFLSLADA
CCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHCCCEEEEEEECHHHEEEHHHH
RERIEDWRCHYNEDRPHTALGGLTPRAFANQAVTAREIA
HHHHHHHHHCCCCCCCCCHHCCCCCHHHHCCCCHHHHCC
>Mature Secondary Structure
MRPAVRREVAGHLQVTYGISERRACQATGFGRSSQRYRKRADPQVALRMRLKELAAARVR
CCCHHHHHHCCCEEEEECCCCHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHH
YGYRRLHILLRREGWEVNHKRTYRIYRDEGLSIRPKLPKRKRAWRYRQGRPAIGGPNEVW
HHHHHHHEEEECCCCCCCCCEEEEEEECCCCEECCCCCCHHHHHHHCCCCCCCCCCCCEE
AMDFMSDRLFDGRPFRILTVVDCHTREALSLTPRANFRAFQVTEALDALVKLRGRPKSLR
EHHHHHHHCCCCCCEEEEEEEECCCCCCEEECCCCCCEEEHHHHHHHHHHHHCCCCCEEE
VDNGPEFAGRMLDQWAYLNGVEIDFSRPGKPTDNAYIESFNGRLRAECLNASWFLSLADA
CCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHCCCEEEEEEECHHHEEEHHHH
RERIEDWRCHYNEDRPHTALGGLTPRAFANQAVTAREIA
HHHHHHHHHCCCCCCCCCHHCCCCCHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]