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The map label for this gene is ackA [H]

Identifier: 170746858

GI number: 170746858

Start: 464872

End: 465987

Strand: Reverse

Name: ackA [H]

Synonym: Mrad2831_0422

Alternate gene names: 170746858

Gene position: 465987-464872 (Counterclockwise)

Preceding gene: 170746859

Following gene: 170746850

Centisome position: 7.67

GC content: 72.76

Gene sequence:

>1116_bases
ATGATCCTCGTCCTCAACGCCGGCTCCTCCAGCCTGCGCTGCGCCCTGTTCCAGCGGGGCGCGGACGGGCCGGCCTGGCG
AGCGCACGTCGCCGGGATCGGGCATGAACCGAGACTCTCGATCGACAGCGTCGCCGTCGCGGGCGAGCGTCCCCCGGCGG
GCGACCATGCCGCCGCAGCCCGCTGGCTGTTCGCGCGACTTCCCGCGGCACCGACCGCGGCGGGGCACCGCGTCGTCCAC
GGCGGCCTGCATCACGCGGCGCCGGTGCGGCTCGACGCGGAGATCCTATCGGCGCTCGAGGGGCTTATTCCGCTCGCGCC
CGCGCACCAGCCGCAGGCGCTCGCCTGCATCCGCGCGTTCGGGGAGGTCTGGCCGGATCTGCCGCAGGTTGCCTGCTTCG
ACACCGCCTTCCATCGGACGCAGGACCGGTTGGCCCAACTCTACCCGCTGCCGCGCGCCCTCATCAACGACGGCCTCGTG
CGCTTCGGCTTCCACGGCCTGTCCTACGCCCATCTCACCCGGGCTTACCCGAAAGGGCGGATTCTGGCCGCCCATCTCGG
GCACGGGGCGAGCCTGTGCGCGATCCGGGACGCGCGAAGCGTCGCCACGACCATGGGCTTCACCACGCTCGACGGGCTGA
TGATGGGCACCCGCAGCGGGGCGGTCGACCCGGGCCTCGTTCTGTACCTGATCCGGGAGCGTGGCCTGTCGGCCGACGCG
GTGGCCGATCTCCTGAACGCGCGGTCGGGCCTCCTCGGCGTCTCCGGCATCAGCGACGACGTGCAGGTTTTGGAGGCGAG
CGACGCCTCTGCCGCGCGTGAGGCCCTGGATCTGTTCACCTACCGGATCGTGCGCGAGGCGGGCTCGCTGATCGCCGCCC
TTGGCGGGGTCGACACCCTCGTGTTCACCGGCGGGATCGGCGAGCACGCCGCGCAGATTCGCGCCGACGTCTGCCGGGGG
CTTGCTTTCGCAGGCGTCGCGCTCGACGAAGTGGCCAACGCCGGCGGCAGCCCGAAGATCGAGGCGTCCGGCTCGGCGGT
CGCGGTGCGCATCGCGCCCGCGGACGAGGAGCGCGAGATCGCCCTGTCGGTGACCGATGCGCTGGGGCCGTCGTGA

Upstream 100 bases:

>100_bases
GATCGAGGGCGACATCATGCAGTGCGGCCCGACCGACACGGCCAAGCCTGGGCAGGCGGGGACGGTGGACGGGTGGGCGG
TCGTAAATTGAGGTCGGGCG

Downstream 100 bases:

>100_bases
GCGCGCCTATCGCCAAAGCATCGCCGGCGCCTTCCGACCTGTTCGGTCAGGGCGTCGCGCGCTCCGGCCTGAGTTAGGCG
GGGTCGAACTCGGCGACCTC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MILVLNAGSSSLRCALFQRGADGPAWRAHVAGIGHEPRLSIDSVAVAGERPPAGDHAAAARWLFARLPAAPTAAGHRVVH
GGLHHAAPVRLDAEILSALEGLIPLAPAHQPQALACIRAFGEVWPDLPQVACFDTAFHRTQDRLAQLYPLPRALINDGLV
RFGFHGLSYAHLTRAYPKGRILAAHLGHGASLCAIRDARSVATTMGFTTLDGLMMGTRSGAVDPGLVLYLIRERGLSADA
VADLLNARSGLLGVSGISDDVQVLEASDASAAREALDLFTYRIVREAGSLIAALGGVDTLVFTGGIGEHAAQIRADVCRG
LAFAGVALDEVANAGGSPKIEASGSAVAVRIAPADEEREIALSVTDALGPS

Sequences:

>Translated_371_residues
MILVLNAGSSSLRCALFQRGADGPAWRAHVAGIGHEPRLSIDSVAVAGERPPAGDHAAAARWLFARLPAAPTAAGHRVVH
GGLHHAAPVRLDAEILSALEGLIPLAPAHQPQALACIRAFGEVWPDLPQVACFDTAFHRTQDRLAQLYPLPRALINDGLV
RFGFHGLSYAHLTRAYPKGRILAAHLGHGASLCAIRDARSVATTMGFTTLDGLMMGTRSGAVDPGLVLYLIRERGLSADA
VADLLNARSGLLGVSGISDDVQVLEASDASAAREALDLFTYRIVREAGSLIAALGGVDTLVFTGGIGEHAAQIRADVCRG
LAFAGVALDEVANAGGSPKIEASGSAVAVRIAPADEEREIALSVTDALGPS
>Mature_371_residues
MILVLNAGSSSLRCALFQRGADGPAWRAHVAGIGHEPRLSIDSVAVAGERPPAGDHAAAARWLFARLPAAPTAAGHRVVH
GGLHHAAPVRLDAEILSALEGLIPLAPAHQPQALACIRAFGEVWPDLPQVACFDTAFHRTQDRLAQLYPLPRALINDGLV
RFGFHGLSYAHLTRAYPKGRILAAHLGHGASLCAIRDARSVATTMGFTTLDGLMMGTRSGAVDPGLVLYLIRERGLSADA
VADLLNARSGLLGVSGISDDVQVLEASDASAAREALDLFTYRIVREAGSLIAALGGVDTLVFTGGIGEHAAQIRADVCRG
LAFAGVALDEVANAGGSPKIEASGSAVAVRIAPADEEREIALSVTDALGPS

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=390, Percent_Identity=34.8717948717949, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI145693184, Length=387, Percent_Identity=34.8837209302326, Blast_Score=193, Evalue=2e-50,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 38477; Mature: 38477

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVLNAGSSSLRCALFQRGADGPAWRAHVAGIGHEPRLSIDSVAVAGERPPAGDHAAAA
CEEEEECCCCCCEEHEECCCCCCCCHHHHCCCCCCCCCCCCCCEEECCCCCCCCCHHHHH
RWLFARLPAAPTAAGHRVVHGGLHHAAPVRLDAEILSALEGLIPLAPAHQPQALACIRAF
HHHHHHCCCCCCCCCCCEEECCCCCCCCCEECHHHHHHHHHHCCCCCCCCCHHHHHHHHH
GEVWPDLPQVACFDTAFHRTQDRLAQLYPLPRALINDGLVRFGFHGLSYAHLTRAYPKGR
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHCCCHHHHHHHCCCCCC
ILAAHLGHGASLCAIRDARSVATTMGFTTLDGLMMGTRSGAVDPGLVLYLIRERGLSADA
EEEEECCCCCEEEEEHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHEEEEEHHCCCCHHH
VADLLNARSGLLGVSGISDDVQVLEASDASAAREALDLFTYRIVREAGSLIAALGGVDTL
HHHHHHHCCCCEECCCCCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VFTGGIGEHAAQIRADVCRGLAFAGVALDEVANAGGSPKIEASGSAVAVRIAPADEEREI
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEECCCCCCEE
ALSVTDALGPS
EEEEECCCCCC
>Mature Secondary Structure
MILVLNAGSSSLRCALFQRGADGPAWRAHVAGIGHEPRLSIDSVAVAGERPPAGDHAAAA
CEEEEECCCCCCEEHEECCCCCCCCHHHHCCCCCCCCCCCCCCEEECCCCCCCCCHHHHH
RWLFARLPAAPTAAGHRVVHGGLHHAAPVRLDAEILSALEGLIPLAPAHQPQALACIRAF
HHHHHHCCCCCCCCCCCEEECCCCCCCCCEECHHHHHHHHHHCCCCCCCCCHHHHHHHHH
GEVWPDLPQVACFDTAFHRTQDRLAQLYPLPRALINDGLVRFGFHGLSYAHLTRAYPKGR
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHCCCHHHHHHHCCCCCC
ILAAHLGHGASLCAIRDARSVATTMGFTTLDGLMMGTRSGAVDPGLVLYLIRERGLSADA
EEEEECCCCCEEEEEHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHEEEEEHHCCCCHHH
VADLLNARSGLLGVSGISDDVQVLEASDASAAREALDLFTYRIVREAGSLIAALGGVDTL
HHHHHHHCCCCEECCCCCCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VFTGGIGEHAAQIRADVCRGLAFAGVALDEVANAGGSPKIEASGSAVAVRIAPADEEREI
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEECCCCCCEE
ALSVTDALGPS
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA