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The map label for this gene is dctA [H]

Identifier: 170746813

GI number: 170746813

Start: 400950

End: 402293

Strand: Reverse

Name: dctA [H]

Synonym: Mrad2831_0367

Alternate gene names: 170746813

Gene position: 402293-400950 (Counterclockwise)

Preceding gene: 170746817

Following gene: 170746810

Centisome position: 6.62

GC content: 66.67

Gene sequence:

>1344_bases
ATGGCTGCCGTGCCATCACCACTCACCGCCCCGCATCCGCCCGCCAGACCGAAGCCGCTGTACCGGACGCTCTACTTTCA
AGTCCTGGTCGCCGTCGCGATCGGCATCCTGCTCGGACATTTCTACCCGCAGCTCGGCGCCGAGTTGAAGCCGCTGGGCG
ACGCCTTCATCAAGCTGGTCAAGATGATCATCGCCCCGGTGATCTTCCTGACCGTCGTCTCCGGCATCGCCGGCATGACC
AATCTCGAGAAGGTCGGCCGGGTCGGCGGCAAGGCCCTGATCTACTTCCTGACGTTCTCGACGCTGGCGCTGATCGTCGG
CCTCGTCGTCGCCAACGTGCTCCAGCCGGGCAACGGCCTGCACATCGACCCGAAGTCCCTGGATCCGAAGGCGGTCGCCA
CCTACGCCGGCAAGGCGAAGGAGCAGAGCATCGTCGACTTCCTGATGAACATCATCCCCACGACGGCGGTCGGCGCCTTC
GCGGGCGGTGAGATCCTGCAGGTGCTGTTCTTCTCGGTGCTGTTCGGTTTCGGCCTCGCCTTCCTGGGCGACCGCGGCAA
GCCGGTGCTCGACATCATCAAGGTCCTGTCCGAGGCGATCTTCGGCGTCGTCAACATCATCATGAAGGTCGCCCCGATCG
GTGCCTTCGGCGCGATGGCGTTCACGATCGGCAAGTACGGGATCAGCTCGCTCGCCAACCTCGCCTACCTCGTCGGCGCC
TTCTACCTGACTTCGGCGATCTTCGTTCTGGTCGTGCTCGGCGCGGTCGCCCGCTACAACGGCTTCTCGATCATCAAGCT
CATCCGCTACATCAAGGAGGAGCTGCTCCTCGTGCTCGGCACGTCGTCGTCCGAGTCGGCGCTGCCCTCCCTGCTGGAGA
AGATGGAGCGGGCCGGCTGCTCGAAGCCGGTCGTCGGCCTCGTGGTGCCCACGGGCTACTCGTTCAACCTCGACGGCACC
AACATCTACATGACCATGGCTGCGCTGTTCATCGCCCAGGCGACCGACACGCCGCTGAGCCTCGGCGAGCAGGCGCTGCT
GCTGCTCGTCGCCATGCTGTCGTCCAAGGGCGCCGCGGGCGTGACCGGCTCGGGCTTCATCACCCTCGCGGCGACGCTCG
CCGTGGTGCCCTCCGTGCCGGTGGTCGGCATGGCGCTGATCCTCGGCGTCGACCGCTTCATGTCGGAGTGCCGCGCGCTG
ACGAACTTCATCGGCAACGCGGTGGCCTGCATCGTCGTGGCGCGCTGGGAGAATGAGGTCGACGAGGCCAAGCTCGCCGC
GGCCCTGTCCGGTCAGACCCTGGCGCCGCTCGCGCCCGCGCCGGCTCTCCAGCCGGCCGAGTGA

Upstream 100 bases:

>100_bases
GAACGGCTGCGCGCCGAAAGGCCAACAGCAAGCGCGGACACCGTCGGCTCACGCCGACGCAAGCCGCCCCATCCGAAGCT
CGCGACAGGGAGAACGCCCC

Downstream 100 bases:

>100_bases
CAAGTCGGCGGCGCGGGCCGCGCTTCGGCGAGGCCCGCGCACATCGTGCGGAACACAGATCGCGCCGCGCCCCGGAGGGA
TCCGCGGCGTGGCGCCCGTA

Product: sodium:dicarboxylate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MAAVPSPLTAPHPPARPKPLYRTLYFQVLVAVAIGILLGHFYPQLGAELKPLGDAFIKLVKMIIAPVIFLTVVSGIAGMT
NLEKVGRVGGKALIYFLTFSTLALIVGLVVANVLQPGNGLHIDPKSLDPKAVATYAGKAKEQSIVDFLMNIIPTTAVGAF
AGGEILQVLFFSVLFGFGLAFLGDRGKPVLDIIKVLSEAIFGVVNIIMKVAPIGAFGAMAFTIGKYGISSLANLAYLVGA
FYLTSAIFVLVVLGAVARYNGFSIIKLIRYIKEELLLVLGTSSSESALPSLLEKMERAGCSKPVVGLVVPTGYSFNLDGT
NIYMTMAALFIAQATDTPLSLGEQALLLLVAMLSSKGAAGVTGSGFITLAATLAVVPSVPVVGMALILGVDRFMSECRAL
TNFIGNAVACIVVARWENEVDEAKLAAALSGQTLAPLAPAPALQPAE

Sequences:

>Translated_447_residues
MAAVPSPLTAPHPPARPKPLYRTLYFQVLVAVAIGILLGHFYPQLGAELKPLGDAFIKLVKMIIAPVIFLTVVSGIAGMT
NLEKVGRVGGKALIYFLTFSTLALIVGLVVANVLQPGNGLHIDPKSLDPKAVATYAGKAKEQSIVDFLMNIIPTTAVGAF
AGGEILQVLFFSVLFGFGLAFLGDRGKPVLDIIKVLSEAIFGVVNIIMKVAPIGAFGAMAFTIGKYGISSLANLAYLVGA
FYLTSAIFVLVVLGAVARYNGFSIIKLIRYIKEELLLVLGTSSSESALPSLLEKMERAGCSKPVVGLVVPTGYSFNLDGT
NIYMTMAALFIAQATDTPLSLGEQALLLLVAMLSSKGAAGVTGSGFITLAATLAVVPSVPVVGMALILGVDRFMSECRAL
TNFIGNAVACIVVARWENEVDEAKLAAALSGQTLAPLAPAPALQPAE
>Mature_446_residues
AAVPSPLTAPHPPARPKPLYRTLYFQVLVAVAIGILLGHFYPQLGAELKPLGDAFIKLVKMIIAPVIFLTVVSGIAGMTN
LEKVGRVGGKALIYFLTFSTLALIVGLVVANVLQPGNGLHIDPKSLDPKAVATYAGKAKEQSIVDFLMNIIPTTAVGAFA
GGEILQVLFFSVLFGFGLAFLGDRGKPVLDIIKVLSEAIFGVVNIIMKVAPIGAFGAMAFTIGKYGISSLANLAYLVGAF
YLTSAIFVLVVLGAVARYNGFSIIKLIRYIKEELLLVLGTSSSESALPSLLEKMERAGCSKPVVGLVVPTGYSFNLDGTN
IYMTMAALFIAQATDTPLSLGEQALLLLVAMLSSKGAAGVTGSGFITLAATLAVVPSVPVVGMALILGVDRFMSECRALT
NFIGNAVACIVVARWENEVDEAKLAAALSGQTLAPLAPAPALQPAE

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI194239697, Length=464, Percent_Identity=24.5689655172414, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4827012, Length=283, Percent_Identity=30.7420494699647, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI169790839, Length=467, Percent_Identity=25.2676659528908, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI21314632, Length=469, Percent_Identity=25.3731343283582, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI66773030, Length=413, Percent_Identity=25.6658595641646, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI5032093, Length=413, Percent_Identity=29.7820823244552, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI40254478, Length=271, Percent_Identity=29.8892988929889, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI223468566, Length=291, Percent_Identity=30.9278350515464, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI223468564, Length=289, Percent_Identity=30.7958477508651, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI262359914, Length=395, Percent_Identity=24.5569620253165, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI301601644, Length=209, Percent_Identity=27.7511961722488, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI1789947, Length=414, Percent_Identity=62.8019323671498, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1790514, Length=421, Percent_Identity=41.5676959619952, Blast_Score=315, Evalue=4e-87,
Organism=Escherichia coli, GI1788024, Length=395, Percent_Identity=24.5569620253165, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789473, Length=373, Percent_Identity=23.0563002680965, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=393, Percent_Identity=26.4631043256997, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI71983099, Length=439, Percent_Identity=24.8291571753986, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71983106, Length=440, Percent_Identity=25, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71996953, Length=422, Percent_Identity=25.3554502369668, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI193206505, Length=453, Percent_Identity=24.0618101545254, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI193206654, Length=455, Percent_Identity=23.5164835164835, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17541374, Length=403, Percent_Identity=23.0769230769231, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583025, Length=418, Percent_Identity=24.8803827751196, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI17137668, Length=418, Percent_Identity=24.8803827751196, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24583023, Length=418, Percent_Identity=24.8803827751196, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI281360481, Length=500, Percent_Identity=23.6, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281360483, Length=500, Percent_Identity=23.6, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137666, Length=500, Percent_Identity=23.6, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46681; Mature: 46550

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAVPSPLTAPHPPARPKPLYRTLYFQVLVAVAIGILLGHFYPQLGAELKPLGDAFIKLV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KMIIAPVIFLTVVSGIAGMTNLEKVGRVGGKALIYFLTFSTLALIVGLVVANVLQPGNGL
HHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
HIDPKSLDPKAVATYAGKAKEQSIVDFLMNIIPTTAVGAFAGGEILQVLFFSVLFGFGLA
CCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FLGDRGKPVLDIIKVLSEAIFGVVNIIMKVAPIGAFGAMAFTIGKYGISSLANLAYLVGA
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FYLTSAIFVLVVLGAVARYNGFSIIKLIRYIKEELLLVLGTSSSESALPSLLEKMERAGC
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCC
SKPVVGLVVPTGYSFNLDGTNIYMTMAALFIAQATDTPLSLGEQALLLLVAMLSSKGAAG
CCCCEEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC
VTGSGFITLAATLAVVPSVPVVGMALILGVDRFMSECRALTNFIGNAVACIVVARWENEV
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
DEAKLAAALSGQTLAPLAPAPALQPAE
HHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA