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The map label for this gene is cmcAX [H]

Identifier: 170746722

GI number: 170746722

Start: 290486

End: 291640

Strand: Reverse

Name: cmcAX [H]

Synonym: Mrad2831_0273

Alternate gene names: 170746722

Gene position: 291640-290486 (Counterclockwise)

Preceding gene: 170746723

Following gene: 170746721

Centisome position: 4.8

GC content: 72.12

Gene sequence:

>1155_bases
ATGAGCCGCCGCGACCGCGCCCCTCTCGCCGCGGCCTTCCTCGTCGCGGCGATGCTGCCCGCGGCGGCGGCCTCGACGCA
GGACGAGCCTGCCGTGCCACCGAGTACCGCGCAACCGGGCACGCAGCCCGCTCAAACCGCCAGGGCGGCGAGCCCCGGCC
TCGCCGGCAAGCTCGGCAATGACGCCGCATGGCGCGCCTACCGCTCCCGGTTCATCACCGATCAGGGTCGCGTGGTGGAC
ACCGCCAACGGTCTGATCAGCCACAGCGAGGGCCAGGGCTACGGCATGCTGCTCGCCGTGGCGGCGGGCGACCGGGTCAG
CTTCGAGCGGATCTGGGGCTGGACCCGCGCCAACCTGATGGTCCGCGGCGACGAATTGCTGGCGTGGCGCTGGTCGCCCG
ACAAGCGGCCGGCGGTGAGCGACATGAACAACGCCACCGACGGGGACATCCTCGTCGCCTGGGCCCTCACCGAGGCGGCC
GAGGCCTGGAACGAGCCGAGCTACCGCACCGCGGCCCGTCGGATCGCCATCGAGTTCGGGCGCAAGACCATCCTGTTCCG
TGACCCGCACGGGGCGCTGCTGCTCCCCGCGGTCTCGGGCTTCTCGGCCCGCGAGCGCCCGGACGGCCCGCTGGTCAACC
TCTCCTACTGGATCTTCCCGGCCTTCCCGCGCCTCGCCCTGGTGGCGCCGGAATACGACTGGGCGGCCCTGACGCGCAGC
GGCCTCTCCCTGCTGCGGCAGTCCCGCTTCGGTCCGAGCAACCTGCCCACCGAGTGGATCTCCGCCAAGGACGCTCCGCA
CCCCGCAACCGGCTTCCCGCCCCTGTTCTCCTACAACGCCATCCGCGTCCCGCTCTATCTCGCCTGGGCTGGGCTGGTGC
GGCCCGACGACCTCGCGCCGTTCCAGGCCCTGTGGTCGAGTTCTGACCGGGAGCGGCTGCCGATCGTCGACACCGGCGAC
GGGCGGAAGGTCGAGTGGCTCACCGAGGCCGGCTACATGGGGATCCCCGCCCTGGTGGCCTGCGCCCTCGACGGCACGCC
CTTTCCGGAGGATGCCCGGGGCGACATCGGCAACCAGAACTATTATCCGGCGACCCTGAGCCTCCTCGCCCTGACCGCGG
CCCGCATGCGCTACCCGTCATGCCTGAAGTCCTGA

Upstream 100 bases:

>100_bases
CGATCAACACGGAATACTACGTCGGCATGACCCTCGCGATGGCCCTCTGCCTCGGCCTCGCGACCACGAGCCTCGTGCGC
AACGTCGGCAGGAAGAACTC

Downstream 100 bases:

>100_bases
AGGGCCCTCCGCCGGCCCGGGTGCGACCGGCCGCCCCCTGCCTCCTACTCCTCGCGCTCGCCGCCTCGCCGGCCGCGGCG
CAGGTCGACGCGCGTTCCGG

Product: cellulase

Products: NA

Alternate protein names: Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSRRDRAPLAAAFLVAAMLPAAAASTQDEPAVPPSTAQPGTQPAQTARAASPGLAGKLGNDAAWRAYRSRFITDQGRVVD
TANGLISHSEGQGYGMLLAVAAGDRVSFERIWGWTRANLMVRGDELLAWRWSPDKRPAVSDMNNATDGDILVAWALTEAA
EAWNEPSYRTAARRIAIEFGRKTILFRDPHGALLLPAVSGFSARERPDGPLVNLSYWIFPAFPRLALVAPEYDWAALTRS
GLSLLRQSRFGPSNLPTEWISAKDAPHPATGFPPLFSYNAIRVPLYLAWAGLVRPDDLAPFQALWSSSDRERLPIVDTGD
GRKVEWLTEAGYMGIPALVACALDGTPFPEDARGDIGNQNYYPATLSLLALTAARMRYPSCLKS

Sequences:

>Translated_384_residues
MSRRDRAPLAAAFLVAAMLPAAAASTQDEPAVPPSTAQPGTQPAQTARAASPGLAGKLGNDAAWRAYRSRFITDQGRVVD
TANGLISHSEGQGYGMLLAVAAGDRVSFERIWGWTRANLMVRGDELLAWRWSPDKRPAVSDMNNATDGDILVAWALTEAA
EAWNEPSYRTAARRIAIEFGRKTILFRDPHGALLLPAVSGFSARERPDGPLVNLSYWIFPAFPRLALVAPEYDWAALTRS
GLSLLRQSRFGPSNLPTEWISAKDAPHPATGFPPLFSYNAIRVPLYLAWAGLVRPDDLAPFQALWSSSDRERLPIVDTGD
GRKVEWLTEAGYMGIPALVACALDGTPFPEDARGDIGNQNYYPATLSLLALTAARMRYPSCLKS
>Mature_383_residues
SRRDRAPLAAAFLVAAMLPAAAASTQDEPAVPPSTAQPGTQPAQTARAASPGLAGKLGNDAAWRAYRSRFITDQGRVVDT
ANGLISHSEGQGYGMLLAVAAGDRVSFERIWGWTRANLMVRGDELLAWRWSPDKRPAVSDMNNATDGDILVAWALTEAAE
AWNEPSYRTAARRIAIEFGRKTILFRDPHGALLLPAVSGFSARERPDGPLVNLSYWIFPAFPRLALVAPEYDWAALTRSG
LSLLRQSRFGPSNLPTEWISAKDAPHPATGFPPLFSYNAIRVPLYLAWAGLVRPDDLAPFQALWSSSDRERLPIVDTGDG
RKVEWLTEAGYMGIPALVACALDGTPFPEDARGDIGNQNYYPATLSLLALTAARMRYPSCLKS

Specific function: Enzyme capable of hydrolyzing carboxy-methyl-cellulose (CMC) [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=245, Percent_Identity=31.0204081632653, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 41718; Mature: 41586

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRDRAPLAAAFLVAAMLPAAAASTQDEPAVPPSTAQPGTQPAQTARAASPGLAGKLGN
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCC
DAAWRAYRSRFITDQGRVVDTANGLISHSEGQGYGMLLAVAAGDRVSFERIWGWTRANLM
HHHHHHHHHHHCCCCCCEEECCCCCEECCCCCCCEEEEEEECCCCCCHHHHHCEEECEEE
VRGDELLAWRWSPDKRPAVSDMNNATDGDILVAWALTEAAEAWNEPSYRTAARRIAIEFG
EECCEEEEEEECCCCCCCHHCCCCCCCCCEEEEEEHHHHHHHHCCCCHHHHHHHHHHCCC
RKTILFRDPHGALLLPAVSGFSARERPDGPLVNLSYWIFPAFPRLALVAPEYDWAALTRS
CEEEEEECCCCCEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCCHHHHHHH
GLSLLRQSRFGPSNLPTEWISAKDAPHPATGFPPLFSYNAIRVPLYLAWAGLVRPDDLAP
HHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCH
FQALWSSSDRERLPIVDTGDGRKVEWLTEAGYMGIPALVACALDGTPFPEDARGDIGNQN
HHHHHCCCCCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
YYPATLSLLALTAARMRYPSCLKS
CCHHHHHHHHHHHHHHHCHHHHCC
>Mature Secondary Structure 
SRRDRAPLAAAFLVAAMLPAAAASTQDEPAVPPSTAQPGTQPAQTARAASPGLAGKLGN
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCC
DAAWRAYRSRFITDQGRVVDTANGLISHSEGQGYGMLLAVAAGDRVSFERIWGWTRANLM
HHHHHHHHHHHCCCCCCEEECCCCCEECCCCCCCEEEEEEECCCCCCHHHHHCEEECEEE
VRGDELLAWRWSPDKRPAVSDMNNATDGDILVAWALTEAAEAWNEPSYRTAARRIAIEFG
EECCEEEEEEECCCCCCCHHCCCCCCCCCEEEEEEHHHHHHHHCCCCHHHHHHHHHHCCC
RKTILFRDPHGALLLPAVSGFSARERPDGPLVNLSYWIFPAFPRLALVAPEYDWAALTRS
CEEEEEECCCCCEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCCHHHHHHH
GLSLLRQSRFGPSNLPTEWISAKDAPHPATGFPPLFSYNAIRVPLYLAWAGLVRPDDLAP
HHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCH
FQALWSSSDRERLPIVDTGDGRKVEWLTEAGYMGIPALVACALDGTPFPEDARGDIGNQN
HHHHHCCCCCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
YYPATLSLLALTAARMRYPSCLKS
CCHHHHHHHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300521 [H]