Click here to switch to the map view.

The map label for this gene is macA [H]

Identifier: 170746674

GI number: 170746674

Start: 238568

End: 239953

Strand: Reverse

Name: macA [H]

Synonym: Mrad2831_0225

Alternate gene names: 170746674

Gene position: 239953-238568 (Counterclockwise)

Preceding gene: 170746675

Following gene: 170746673

Centisome position: 3.95

GC content: 72.44

Gene sequence:

>1386_bases
ATGACCGACTTCAACGACGCTCCCGGGAGCGGCGCCGAAGCCGCCGCGCGGGCGCACCGGCACGCGGTCGAGATCGACCC
GCTGCGGCAGGACCAGGCCGGCGAGGTGCTGGTCCACGCCGTGCCGCCGCCGCCGCGCAAGCGCCCGTTCCTCGTGGCGC
TCCTGGCGCTCGGAAGCGCCCTCGGGCTCGGCGCCTGGCGCCACTGGGAAACCTATCAGGCTTCCCTGGTGACGCAGTCG
GAGGCCGTGAACTTCGTGCCCAACGTCCGCACCGGGCCGGCGCAGGTGGATGACGGGCCGATGATGCTGACCCTGCCGGG
CCAGACCGAGGCCTTCGACCATGCCGACATCTTCGCCCGCGCCACCGGCTACATCGCCGAGCGCCGGGTCGACATCGGGT
CGCGGGTGAAGAAGGGCGATCTCCTGGTCCGGATCACCGCGCCCGACCTCGATCAGCAGGCCCGGCAGGCCGAGGCCCAA
CTCGGCCAGTTCGAGGCGCAGCTCGCGGTCGCGCGGGCCGACGTGGAGCAGGCCGAGGCCAGCGTGACGCTCGCGAACGT
CACCGATGCCCGCACCGCGACCCTGGCCGGGCAAGGCTGGGCCTCGAAGCAGAATGCCGACCAGACCCGGGCCGGGATGC
TGACCGCCACCGCGTCACTCGCCGCCGCGCAGGCGAAGGTGAAGGTCGCGGAGGCCAACATCACCGCCCAGCAGGCGACC
GTCGACCGGCTCCGGACGCTGACCACATTCGAGAACGTCGTCGCGCCGTTCGACGGCATCGTGACCGCCCGCAAAGTCGA
TGTCGGCGACCTCGTCCACGGCGATGCCGGCTCCGGGACGCCGCTCTTCACGATCGACAGCACCGATGTGCTGCGCGTCG
CGGTGCAGGTGCCGCAATCGGCCGCCGTGGGCATCCGCGACGGGGTGAAGGCGCGCGTTCAGGTCGCGCAGATCCCGGAC
CGCGCGTTCTCCGGCTCGGTGTCGCGCAGCTCGGTCGCTCTCCGATCCTCGGTGCGGACGCTCGACACCGAGGTCGACGT
CGCCAACCCGGACGGGGTTCTCCGGCCCGGTCTCTACGTCGCCGTCACCTTCGCGGTGCCGCGCACGCATCCGCGCGTGA
TCGTGCCGTCCGAGGCGCTGATCTTCGACGCGGACGGGACGCGGGTCGCCGTGGTCGGGGCCGACGGCACCGTCCGGCTG
CGCCCGATCACGATCGCGCGCGATCTCGGCACGCGCCTCGAATTGCGCAGCGGCCTCGAGAGCGGCGACACCGTGGTCCT
CAACCCGCCCGCCGGCCTCCAGGACGGCAGCCGCGTCAACCCCAGGAGTTCCGTCGCCTCGCAGATCCCGACAGCCAAGC
TCTCAGAGCAGGCGAGCGCGGAGTGA

Upstream 100 bases:

>100_bases
TGGGCGGGATCGGGATGGGCACCGTCTCGACCCTGCTGTTCGTCCCCCTTCTCTACACCCTGCTCCGCCGCAACCCGATC
AAGCCCCTCGAGGATTATTG

Downstream 100 bases:

>100_bases
GGCGCATGGTCCAGATCCCCCGCTCTCCCGTAAGCTTTGCCGCCCCGATGATGCAGACCGCCGGCCGCGATCGGACCCCA
TTTGACTCCGGGACCGAAGA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTDFNDAPGSGAEAAARAHRHAVEIDPLRQDQAGEVLVHAVPPPPRKRPFLVALLALGSALGLGAWRHWETYQASLVTQS
EAVNFVPNVRTGPAQVDDGPMMLTLPGQTEAFDHADIFARATGYIAERRVDIGSRVKKGDLLVRITAPDLDQQARQAEAQ
LGQFEAQLAVARADVEQAEASVTLANVTDARTATLAGQGWASKQNADQTRAGMLTATASLAAAQAKVKVAEANITAQQAT
VDRLRTLTTFENVVAPFDGIVTARKVDVGDLVHGDAGSGTPLFTIDSTDVLRVAVQVPQSAAVGIRDGVKARVQVAQIPD
RAFSGSVSRSSVALRSSVRTLDTEVDVANPDGVLRPGLYVAVTFAVPRTHPRVIVPSEALIFDADGTRVAVVGADGTVRL
RPITIARDLGTRLELRSGLESGDTVVLNPPAGLQDGSRVNPRSSVASQIPTAKLSEQASAE

Sequences:

>Translated_461_residues
MTDFNDAPGSGAEAAARAHRHAVEIDPLRQDQAGEVLVHAVPPPPRKRPFLVALLALGSALGLGAWRHWETYQASLVTQS
EAVNFVPNVRTGPAQVDDGPMMLTLPGQTEAFDHADIFARATGYIAERRVDIGSRVKKGDLLVRITAPDLDQQARQAEAQ
LGQFEAQLAVARADVEQAEASVTLANVTDARTATLAGQGWASKQNADQTRAGMLTATASLAAAQAKVKVAEANITAQQAT
VDRLRTLTTFENVVAPFDGIVTARKVDVGDLVHGDAGSGTPLFTIDSTDVLRVAVQVPQSAAVGIRDGVKARVQVAQIPD
RAFSGSVSRSSVALRSSVRTLDTEVDVANPDGVLRPGLYVAVTFAVPRTHPRVIVPSEALIFDADGTRVAVVGADGTVRL
RPITIARDLGTRLELRSGLESGDTVVLNPPAGLQDGSRVNPRSSVASQIPTAKLSEQASAE
>Mature_460_residues
TDFNDAPGSGAEAAARAHRHAVEIDPLRQDQAGEVLVHAVPPPPRKRPFLVALLALGSALGLGAWRHWETYQASLVTQSE
AVNFVPNVRTGPAQVDDGPMMLTLPGQTEAFDHADIFARATGYIAERRVDIGSRVKKGDLLVRITAPDLDQQARQAEAQL
GQFEAQLAVARADVEQAEASVTLANVTDARTATLAGQGWASKQNADQTRAGMLTATASLAAAQAKVKVAEANITAQQATV
DRLRTLTTFENVVAPFDGIVTARKVDVGDLVHGDAGSGTPLFTIDSTDVLRVAVQVPQSAAVGIRDGVKARVQVAQIPDR
AFSGSVSRSSVALRSSVRTLDTEVDVANPDGVLRPGLYVAVTFAVPRTHPRVIVPSEALIFDADGTRVAVVGADGTVRLR
PITIARDLGTRLELRSGLESGDTVVLNPPAGLQDGSRVNPRSSVASQIPTAKLSEQASAE

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=329, Percent_Identity=28.8753799392097, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789929, Length=409, Percent_Identity=25.4278728606357, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87082036, Length=368, Percent_Identity=24.7282608695652, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786668, Length=430, Percent_Identity=26.5116279069767, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790519, Length=249, Percent_Identity=27.710843373494, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 48525; Mature: 48394

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFNDAPGSGAEAAARAHRHAVEIDPLRQDQAGEVLVHAVPPPPRKRPFLVALLALGSA
CCCCCCCCCCCHHHHHHHHHCEEEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHH
LGLGAWRHWETYQASLVTQSEAVNFVPNVRTGPAQVDDGPMMLTLPGQTEAFDHADIFAR
HCCCCCCCCHHHHHHEEEHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHH
ATGYIAERRVDIGSRVKKGDLLVRITAPDLDQQARQAEAQLGQFEAQLAVARADVEQAEA
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SVTLANVTDARTATLAGQGWASKQNADQTRAGMLTATASLAAAQAKVKVAEANITAQQAT
EEEEEECCCCCEEEEECCCCCCCCCCCHHHHCEEEHHHHHHHHHHEEEEEECCCCHHHHH
VDRLRTLTTFENVVAPFDGIVTARKVDVGDLVHGDAGSGTPLFTIDSTDVLRVAVQVPQS
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCEEEEECCHHEEEEEEECCCC
AAVGIRDGVKARVQVAQIPDRAFSGSVSRSSVALRSSVRTLDTEVDVANPDGVLRPGLYV
CCCCCCCCCEEEEEEEECCCHHHCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCEEE
AVTFAVPRTHPRVIVPSEALIFDADGTRVAVVGADGTVRLRPITIARDLGTRLELRSGLE
EEEEECCCCCCEEEECCCEEEEECCCCEEEEEECCCEEEEEEEEEEHHCCCHHHHHCCCC
SGDTVVLNPPAGLQDGSRVNPRSSVASQIPTAKLSEQASAE
CCCEEEECCCCCCCCCCCCCCHHHHHHHCCCHHHCHHCCCC
>Mature Secondary Structure 
TDFNDAPGSGAEAAARAHRHAVEIDPLRQDQAGEVLVHAVPPPPRKRPFLVALLALGSA
CCCCCCCCCCHHHHHHHHHCEEEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHH
LGLGAWRHWETYQASLVTQSEAVNFVPNVRTGPAQVDDGPMMLTLPGQTEAFDHADIFAR
HCCCCCCCCHHHHHHEEEHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHH
ATGYIAERRVDIGSRVKKGDLLVRITAPDLDQQARQAEAQLGQFEAQLAVARADVEQAEA
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SVTLANVTDARTATLAGQGWASKQNADQTRAGMLTATASLAAAQAKVKVAEANITAQQAT
EEEEEECCCCCEEEEECCCCCCCCCCCHHHHCEEEHHHHHHHHHHEEEEEECCCCHHHHH
VDRLRTLTTFENVVAPFDGIVTARKVDVGDLVHGDAGSGTPLFTIDSTDVLRVAVQVPQS
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCEEEEECCHHEEEEEEECCCC
AAVGIRDGVKARVQVAQIPDRAFSGSVSRSSVALRSSVRTLDTEVDVANPDGVLRPGLYV
CCCCCCCCCEEEEEEEECCCHHHCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCEEE
AVTFAVPRTHPRVIVPSEALIFDADGTRVAVVGADGTVRLRPITIARDLGTRLELRSGLE
EEEEECCCCCCEEEECCCEEEEECCCCEEEEEECCCEEEEEEEEEEHHCCCHHHHHCCCC
SGDTVVLNPPAGLQDGSRVNPRSSVASQIPTAKLSEQASAE
CCCEEEECCCCCCCCCCCCCCHHHHHHHCCCHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]