Click here to switch to the map view.

The map label for this gene is pgk

Identifier: 170746608

GI number: 170746608

Start: 157285

End: 158487

Strand: Reverse

Name: pgk

Synonym: Mrad2831_0158

Alternate gene names: 170746608

Gene position: 158487-157285 (Counterclockwise)

Preceding gene: 170746609

Following gene: 170746607

Centisome position: 2.61

GC content: 71.49

Gene sequence:

>1203_bases
ATGAGCGCCTTCCGCACCCTCGACGATGCCGGCTCCCTCAAGGGCAAGCGGGTCCTCATGCGCGTCGACCTGAACGTGCC
CATGGAGAGCGGGCGCGTCACCGACGCGACCCGCATCGAGCGCGTCGCACCGACGATCCGCGAGGTTGCCGAGCAGGGCG
CCAAGGTGATCCTGCTCGCCCATTTCGGTCGACCGAAGGGCAAGCGCGAGCCGAAGGATTCGCTCGAGCCGGTGGTTCCC
GCCCTCGGCGCGGCCCTCGGACGCGATGTCGCCTTCGCGGCTGACTGCGTCGGCGACGCCGCCGCGCAGGCGGTGGCGGC
CCTCAAGGACGGCGACGTCCTCCTCCTCGAGAACACCCGCTACCACGCGGGTGAAGAGAAGAACGACGCGGCCTTCGCGG
ACGCCCTGGCGGCGAACGGCGACGTCTACGTCAACGAGGCCTTCTCGGCGGCCCACCGCGCGCACGCCTCCACCGAGGGT
CTGGCGCACCGGTTGCCGGCCTATGCCGGCCGCGAGATGCAGGCCGAGCTCGATGCGCTGACCAAGGGCCTGGAATCGCC
CCAGCGCCCGGTGATCGCGCTGGTCGGAGGGGCCAAGGTCTCGTCCAAGATCGATCTCCTGGAGAACCTCGTCGCCAAGG
TCGACATGCTGGTGATCGGCGGCGGCATGGCCAACACCTTCCTGCACGCGCAGGGGAAGGCGGTGGGCAAGTCCCTCTGC
GAGAAGGATCTCGCCGAGACCGCCACGCGGATCCTGGCGGCGGCCGAGAAGGCCGGCTGCCGGATCATCCTGCCGGTCGA
CGCGGTCGCCGCGAGCGAGTTCAAGGCGCGGGCCCCCCACGAGACTGTGCCGGTGGACGCCGTGCCGGACGCCAGCATGA
TCCTCGATGCCGGTCCGCGCTCGGTCGCCGAGATCAACGCCGCCATCGACGGGGCCAAGACCCTGGTGTGGAACGGCCCG
CTGGGCGCCTTCGAGCTCGCCCCGTTCGATGCCGCGACCGTGGCCACCGCCAAGCACGCGGCCGCGCGGACGAAGGATGG
CCAGCTCGTCTCGGTCGCGGGCGGCGGCGACACGGTGGCCGCGCTCAACCATGCCGGCGTGGGGCAGGACTTCACCTACG
TCTCGACCGCCGGCGGCGCCTTCCTCGAGTGGCTGGAAGGCAAGGCCCTCCCGGGCGTCGAGGCGCTGCGGGCGAAAGGC
TGA

Upstream 100 bases:

>100_bases
GTCCAGCGGCTCAACCTCGACCGTCTGCTGCCGCAGCGGGCCACCATCGGCACCACCGCGCAGTAATCGCCCCGCCTCGC
ACAGGACCGCACCAGACCGC

Downstream 100 bases:

>100_bases
GAGGGCCGCCTTGCTCTCCTCCGCCTGCGACACGACCCTATCTACATGGCCGGCCGTCGTCGGCCGGGGGGCCGAGGCCA
GGGGTGACCACGTGGAGATC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSAFRTLDDAGSLKGKRVLMRVDLNVPMESGRVTDATRIERVAPTIREVAEQGAKVILLAHFGRPKGKREPKDSLEPVVP
ALGAALGRDVAFAADCVGDAAAQAVAALKDGDVLLLENTRYHAGEEKNDAAFADALAANGDVYVNEAFSAAHRAHASTEG
LAHRLPAYAGREMQAELDALTKGLESPQRPVIALVGGAKVSSKIDLLENLVAKVDMLVIGGGMANTFLHAQGKAVGKSLC
EKDLAETATRILAAAEKAGCRIILPVDAVAASEFKARAPHETVPVDAVPDASMILDAGPRSVAEINAAIDGAKTLVWNGP
LGAFELAPFDAATVATAKHAAARTKDGQLVSVAGGGDTVAALNHAGVGQDFTYVSTAGGAFLEWLEGKALPGVEALRAKG

Sequences:

>Translated_400_residues
MSAFRTLDDAGSLKGKRVLMRVDLNVPMESGRVTDATRIERVAPTIREVAEQGAKVILLAHFGRPKGKREPKDSLEPVVP
ALGAALGRDVAFAADCVGDAAAQAVAALKDGDVLLLENTRYHAGEEKNDAAFADALAANGDVYVNEAFSAAHRAHASTEG
LAHRLPAYAGREMQAELDALTKGLESPQRPVIALVGGAKVSSKIDLLENLVAKVDMLVIGGGMANTFLHAQGKAVGKSLC
EKDLAETATRILAAAEKAGCRIILPVDAVAASEFKARAPHETVPVDAVPDASMILDAGPRSVAEINAAIDGAKTLVWNGP
LGAFELAPFDAATVATAKHAAARTKDGQLVSVAGGGDTVAALNHAGVGQDFTYVSTAGGAFLEWLEGKALPGVEALRAKG
>Mature_399_residues
SAFRTLDDAGSLKGKRVLMRVDLNVPMESGRVTDATRIERVAPTIREVAEQGAKVILLAHFGRPKGKREPKDSLEPVVPA
LGAALGRDVAFAADCVGDAAAQAVAALKDGDVLLLENTRYHAGEEKNDAAFADALAANGDVYVNEAFSAAHRAHASTEGL
AHRLPAYAGREMQAELDALTKGLESPQRPVIALVGGAKVSSKIDLLENLVAKVDMLVIGGGMANTFLHAQGKAVGKSLCE
KDLAETATRILAAAEKAGCRIILPVDAVAASEFKARAPHETVPVDAVPDASMILDAGPRSVAEINAAIDGAKTLVWNGPL
GAFELAPFDAATVATAKHAAARTKDGQLVSVAGGGDTVAALNHAGVGQDFTYVSTAGGAFLEWLEGKALPGVEALRAKG

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=412, Percent_Identity=43.6893203883495, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI31543397, Length=416, Percent_Identity=42.3076923076923, Blast_Score=281, Evalue=6e-76,
Organism=Escherichia coli, GI1789294, Length=403, Percent_Identity=43.424317617866, Blast_Score=287, Evalue=8e-79,
Organism=Caenorhabditis elegans, GI17508823, Length=415, Percent_Identity=44.578313253012, Blast_Score=310, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6319857, Length=405, Percent_Identity=42.4691358024691, Blast_Score=279, Evalue=7e-76,
Organism=Drosophila melanogaster, GI17136394, Length=407, Percent_Identity=41.7690417690418, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24581203, Length=402, Percent_Identity=40.547263681592, Blast_Score=267, Evalue=1e-71,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_METRJ (B1M787)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001752868.1
- ProteinModelPortal:   B1M787
- SMR:   B1M787
- GeneID:   6136458
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_0158
- HOGENOM:   HBG453500
- OMA:   RITEFLP
- ProtClustDB:   CLSK2331121
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41311; Mature: 41180

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 38-38 BINDING 120-120 BINDING 153-153 BINDING 203-203 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFRTLDDAGSLKGKRVLMRVDLNVPMESGRVTDATRIERVAPTIREVAEQGAKVILLA
CCCHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
HFGRPKGKREPKDSLEPVVPALGAALGRDVAFAADCVGDAAAQAVAALKDGDVLLLENTR
ECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
YHAGEEKNDAAFADALAANGDVYVNEAFSAAHRAHASTEGLAHRLPAYAGREMQAELDAL
CCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHCHHHHHHHHCHHHCCCHHHHHHHHH
TKGLESPQRPVIALVGGAKVSSKIDLLENLVAKVDMLVIGGGMANTFLHAQGKAVGKSLC
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHEECCCHHHHHHHH
EKDLAETATRILAAAEKAGCRIILPVDAVAASEFKARAPHETVPVDAVPDASMILDAGPR
HHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCHHEECCCCC
SVAEINAAIDGAKTLVWNGPLGAFELAPFDAATVATAKHAAARTKDGQLVSVAGGGDTVA
HHHHHHHHHCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEE
ALNHAGVGQDFTYVSTAGGAFLEWLEGKALPGVEALRAKG
EECCCCCCCCCEEEECCCHHHHHHHCCCCCCCHHHHHCCC
>Mature Secondary Structure 
SAFRTLDDAGSLKGKRVLMRVDLNVPMESGRVTDATRIERVAPTIREVAEQGAKVILLA
CCHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
HFGRPKGKREPKDSLEPVVPALGAALGRDVAFAADCVGDAAAQAVAALKDGDVLLLENTR
ECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
YHAGEEKNDAAFADALAANGDVYVNEAFSAAHRAHASTEGLAHRLPAYAGREMQAELDAL
CCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHCHHHHHHHHCHHHCCCHHHHHHHHH
TKGLESPQRPVIALVGGAKVSSKIDLLENLVAKVDMLVIGGGMANTFLHAQGKAVGKSLC
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHEECCCHHHHHHHH
EKDLAETATRILAAAEKAGCRIILPVDAVAASEFKARAPHETVPVDAVPDASMILDAGPR
HHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCHHEECCCCC
SVAEINAAIDGAKTLVWNGPLGAFELAPFDAATVATAKHAAARTKDGQLVSVAGGGDTVA
HHHHHHHHHCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEE
ALNHAGVGQDFTYVSTAGGAFLEWLEGKALPGVEALRAKG
EECCCCCCCCCEEEECCCHHHHHHHCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA