Click here to switch to the map view.

The map label for this gene is rlmE [H]

Identifier: 170746591

GI number: 170746591

Start: 144167

End: 144892

Strand: Reverse

Name: rlmE [H]

Synonym: Mrad2831_0141

Alternate gene names: 170746591

Gene position: 144892-144167 (Counterclockwise)

Preceding gene: 170746592

Following gene: 170746590

Centisome position: 2.38

GC content: 73.0

Gene sequence:

>726_bases
ATGAGCGACCGGCGCGGCGGCGCGAGTTCCGGCGGCGGTGTCCGGGGCGAACTGAAGCAGCGGGTGAAGACCGGCCGCGG
ACGGACCCTGTCCCAGAAGCGCTGGCTCGAGCGCCAGCTCAACGATCCCTACGTGGCCCGCGCCAAGCGCGAGGGCTACC
GGTCCCGGGCGGCCTACAAGCTCCTGGAGATCGACGAGCGCTACAAGCTCCTGCGCCCCGGCCAGAAGATCGTCGATCTC
GGCGCCGCGCCGGGGGGCTGGTCGCAGGTGGCCGCGCGGGTGGTCGGGCCGACGGGCCGCGTCGTGGGCATCGATCTCCT
GGAGATCGAGCCGATGGCCGGGGTCGAGTTCATCACCCTCGACTTCCTCGACCCCGAGGCGCCTGACCGTCTGACCGGGA
TGCTCGGCGGCCCCGCCGACCTCGTCATGTCCGACATGGCCGCCAACGCCACCGGTCACAAGAAGACCGACCACCTGCGG
ATCATCGGCCTCGCCGAGACGGCGGCGGAGTTCGCCCGCGAGATCCTGGGCCCCGGAGGCGCCTACCTCGCCAAGGTGCT
CCAGGGCGGCACCGAGGGCGCGCTGCTCACCGACCTGAAGCGCGACTTCGCCACGGTCCGGCACGTCAAGCCGGCCGCGA
GCCGGGCCGATTCGAGCGAGCTCTACGTCCTGGCGACCGGCTACCGGGGCGCGCCGGGCCGGGCGGCGTCCGTCGACGAG
GGCTGA

Upstream 100 bases:

>100_bases
CTGCGCATCGCCGATCGCGGCCTGCGCGAAGGATTGCTGATGAACATGATGCGGGAGGACGGGGTCTGGAACCGGAAGGC
GGTCCGATGAGGGCGGCGCG

Downstream 100 bases:

>100_bases
GCCGCCCGGCGATGCTCCTCGCCTGGGCCCTGTCCGCCCTCAACCCGGCGCCGCTTCCCCTGCGCCGGCTCGGCTATGTC
CGCCAGAGCGGCCTGCTCCA

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MSDRRGGASSGGGVRGELKQRVKTGRGRTLSQKRWLERQLNDPYVARAKREGYRSRAAYKLLEIDERYKLLRPGQKIVDL
GAAPGGWSQVAARVVGPTGRVVGIDLLEIEPMAGVEFITLDFLDPEAPDRLTGMLGGPADLVMSDMAANATGHKKTDHLR
IIGLAETAAEFAREILGPGGAYLAKVLQGGTEGALLTDLKRDFATVRHVKPAASRADSSELYVLATGYRGAPGRAASVDE
G

Sequences:

>Translated_241_residues
MSDRRGGASSGGGVRGELKQRVKTGRGRTLSQKRWLERQLNDPYVARAKREGYRSRAAYKLLEIDERYKLLRPGQKIVDL
GAAPGGWSQVAARVVGPTGRVVGIDLLEIEPMAGVEFITLDFLDPEAPDRLTGMLGGPADLVMSDMAANATGHKKTDHLR
IIGLAETAAEFAREILGPGGAYLAKVLQGGTEGALLTDLKRDFATVRHVKPAASRADSSELYVLATGYRGAPGRAASVDE
G
>Mature_240_residues
SDRRGGASSGGGVRGELKQRVKTGRGRTLSQKRWLERQLNDPYVARAKREGYRSRAAYKLLEIDERYKLLRPGQKIVDLG
AAPGGWSQVAARVVGPTGRVVGIDLLEIEPMAGVEFITLDFLDPEAPDRLTGMLGGPADLVMSDMAANATGHKKTDHLRI
IGLAETAAEFAREILGPGGAYLAKVLQGGTEGALLTDLKRDFATVRHVKPAASRADSSELYVLATGYRGAPGRAASVDEG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=218, Percent_Identity=45.4128440366972, Blast_Score=188, Evalue=4e-48,
Organism=Homo sapiens, GI29029591, Length=205, Percent_Identity=36.0975609756098, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI29029589, Length=205, Percent_Identity=36.0975609756098, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI7110661, Length=205, Percent_Identity=36.0975609756098, Blast_Score=117, Evalue=9e-27,
Organism=Homo sapiens, GI194097365, Length=197, Percent_Identity=32.994923857868, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1789569, Length=205, Percent_Identity=42.9268292682927, Blast_Score=171, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17553474, Length=202, Percent_Identity=36.1386138613861, Blast_Score=147, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71987550, Length=193, Percent_Identity=34.1968911917098, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71987561, Length=201, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17553860, Length=201, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17554650, Length=200, Percent_Identity=31, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6319535, Length=209, Percent_Identity=31.5789473684211, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6321302, Length=101, Percent_Identity=48.5148514851485, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=195, Percent_Identity=31.2820512820513, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356387, Length=223, Percent_Identity=39.4618834080717, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI18859957, Length=200, Percent_Identity=34.5, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24647580, Length=213, Percent_Identity=31.924882629108, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24648639, Length=211, Percent_Identity=34.1232227488152, Blast_Score=103, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 25767; Mature: 25636

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRRGGASSGGGVRGELKQRVKTGRGRTLSQKRWLERQLNDPYVARAKREGYRSRAAYK
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LLEIDERYKLLRPGQKIVDLGAAPGGWSQVAARVVGPTGRVVGIDLLEIEPMAGVEFITL
HHHHHHHHHHHCCCHHHEECCCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEE
DFLDPEAPDRLTGMLGGPADLVMSDMAANATGHKKTDHLRIIGLAETAAEFAREILGPGG
ECCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCH
AYLAKVLQGGTEGALLTDLKRDFATVRHVKPAASRADSSELYVLATGYRGAPGRAASVDE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
G
C
>Mature Secondary Structure 
SDRRGGASSGGGVRGELKQRVKTGRGRTLSQKRWLERQLNDPYVARAKREGYRSRAAYK
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LLEIDERYKLLRPGQKIVDLGAAPGGWSQVAARVVGPTGRVVGIDLLEIEPMAGVEFITL
HHHHHHHHHHHCCCHHHEECCCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEE
DFLDPEAPDRLTGMLGGPADLVMSDMAANATGHKKTDHLRIIGLAETAAEFAREILGPGG
ECCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCH
AYLAKVLQGGTEGALLTDLKRDFATVRHVKPAASRADSSELYVLATGYRGAPGRAASVDE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA