Click here to switch to the map view.

The map label for this gene is rlmH

Identifier: 170746550

GI number: 170746550

Start: 106750

End: 107244

Strand: Reverse

Name: rlmH

Synonym: Mrad2831_0100

Alternate gene names: 170746550

Gene position: 107244-106750 (Counterclockwise)

Preceding gene: 170746551

Following gene: 170746549

Centisome position: 1.76

GC content: 72.73

Gene sequence:

>495_bases
ATGCGCCTGATCCTGGCCGCGGTCGGACGGCTGAAGGCCGGGCCGGAGCGCGAGCTCGCGTCACGATATCGCGACCGCGC
CGCACAACTCGGACGCGGCCTCGGCTTCCCGTCCTGCGACAACGTCGAGATTCCGGAATCCCGGGCCCGTCGCGCCCCCG
ACCGGTGCGCCGAGGAGGCGGCGGCGCTCGCGACCTATCTGCCCTCCGGCGGAGTCCTCGTCGCCTACGACGAGCGCGGC
CGGGCCGATCTCGGCAGCGAGGCCCTGGCAGAACGCGTCGCCGGCTGGCGCGATGCGGCCCGGCCGGCTCTGGTCGTGAC
GATCGGTGGGCCGGACGGGCTGGACGCCTCAATTCGGACGCGGGCCGAGTTGATCCTGTCGTTCGGGGCCGCCACCCTGC
CCCACGGACTCGTTCGCGTGCTCGCCCTCGAACAACTCTATCGCAGCCTCACGATCCTGGCCGGACACCCCTACCATCGC
GGTGAGGCGGGCTGA

Upstream 100 bases:

>100_bases
CATCTTCCGCCCCGAAGTCCGCGGCTTCTACAATCTCGAGAAGATGTGGGGCGCCGACCGTCCCGTCGGGCTCGCGGCCG
GCTAGGCCGGACCGAGGTCC

Downstream 100 bases:

>100_bases
ACGGCCGTGGGGAAGTCCGAGACCGAGGCCGAGTCCAACCCCGTGCCGCGGCGCGGACGCGCGGCATTCGCCCTGCTGGC
CGTGCTGTCGATCACCGTGG

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MRLILAAVGRLKAGPERELASRYRDRAAQLGRGLGFPSCDNVEIPESRARRAPDRCAEEAAALATYLPSGGVLVAYDERG
RADLGSEALAERVAGWRDAARPALVVTIGGPDGLDASIRTRAELILSFGAATLPHGLVRVLALEQLYRSLTILAGHPYHR
GEAG

Sequences:

>Translated_164_residues
MRLILAAVGRLKAGPERELASRYRDRAAQLGRGLGFPSCDNVEIPESRARRAPDRCAEEAAALATYLPSGGVLVAYDERG
RADLGSEALAERVAGWRDAARPALVVTIGGPDGLDASIRTRAELILSFGAATLPHGLVRVLALEQLYRSLTILAGHPYHR
GEAG
>Mature_164_residues
MRLILAAVGRLKAGPERELASRYRDRAAQLGRGLGFPSCDNVEIPESRARRAPDRCAEEAAALATYLPSGGVLVAYDERG
RADLGSEALAERVAGWRDAARPALVVTIGGPDGLDASIRTRAELILSFGAATLPHGLVRVLALEQLYRSLTILAGHPYHR
GEAG

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=88, Percent_Identity=40.9090909090909, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_METRJ (B1M692)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001752810.1
- ProteinModelPortal:   B1M692
- SMR:   B1M692
- GeneID:   6136400
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_0100
- HOGENOM:   HBG622807
- OMA:   ILSGHPY
- ProtClustDB:   PRK00103
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17466; Mature: 17466

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLILAAVGRLKAGPERELASRYRDRAAQLGRGLGFPSCDNVEIPESRARRAPDRCAEEA
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHHHH
AALATYLPSGGVLVAYDERGRADLGSEALAERVAGWRDAARPALVVTIGGPDGLDASIRT
HHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
RAELILSFGAATLPHGLVRVLALEQLYRSLTILAGHPYHRGEAG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRLILAAVGRLKAGPERELASRYRDRAAQLGRGLGFPSCDNVEIPESRARRAPDRCAEEA
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCHHHHHHHH
AALATYLPSGGVLVAYDERGRADLGSEALAERVAGWRDAARPALVVTIGGPDGLDASIRT
HHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHH
RAELILSFGAATLPHGLVRVLALEQLYRSLTILAGHPYHRGEAG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA