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The map label for this gene is hsrA [H]

Identifier: 170746532

GI number: 170746532

Start: 88539

End: 90038

Strand: Reverse

Name: hsrA [H]

Synonym: Mrad2831_0082

Alternate gene names: 170746532

Gene position: 90038-88539 (Counterclockwise)

Preceding gene: 170746536

Following gene: 170746527

Centisome position: 1.48

GC content: 70.93

Gene sequence:

>1500_bases
GTGGCCGCGCTGCCGTGCAACCGCGGCGGCGCGCCATGCGTCTGCTGTGTGATGCCTGCCACCGCCCAAAGACCGCTCTG
GATCGTCCCCCTGACGGTCGCCACCGCGCTGTTCATGGAGAACACCGATTCCACGGTGATCGCCACGGCGCTCCCGAGCA
TCGCGGCGAGCCTTCACGAGGACCCGATCGCCCTCAAGCTCGCGCTGACCTCCTACCTGGTCAGCCTCGCGATCTTCATC
CCCGTAAGCGGCTGGATGGCGGACCGGTACGGGTCGCGGACCGTGTTCCGCGCGGCCCTCGTGGTGTTCATGGCGGGCTC
GCTCGCCTGCGCGGCCGCGAACGGGCTCGCGTGGTTCGTCGCCGCGCGGTTCCTCCAGGGCATGGGCGGCGCCATGATGG
TGCCGGTCGGACGGCTCGTGGTGCTGCGCAGCGTGCCGAAATCGCAGCTTGTCACGGCGCTGGCCTACCTGACCTTCCCG
GCCCTCGTCGGCCCGGTGCTGGGTCCGCCGGTGGGCGGCCTGATCACCACGTGGTTCGACTGGCGCTGGATCTTCTTCAT
CAACCTGCCGATCGGCTGCGCCGGAATCGTCCTGGCGAGCCTGTACTTCGAGGATGTGCGCGAGCCGGAGCGGCCGCCCC
TCGATCTCCTCGGCTTCGTGCTGCTCGGGACCGGCCTCGCCGCCCTGATGCTCGGCCTCGCCTCCCTCGGCCGCCACCTG
CTGCCGGCGGCCGTCTCGTGGGGCTGCCTCGCCGCGGGCGCGGTGCTCCTGCCCCTCTACTGGGCGCATGCCCGGCGGGT
GGCCTACCCGATCGTCCGGCTCGACCTGATGCAGCGGCCGACCTTCCGAGTCGCGGTGCTCGGCGGCAGCCTGTTCCGGA
TCGGCACCGGTGCCATCCCGTTCCTGCTGCCGCTGATGCTGCAGGTCGGCTTCGGGCTCGACGCGCTGCAGTCCGGCCTG
ATCACCTTCGCGGCGGCGGCCGGCGCGATGTTCGTGAAGACCCTGGGTCCGCGAATCCTGCGCCGCACCGGCTTCCGCGC
GCTGATGGTCTGGAACGCGCTGCTCGCCTCGGCCTTCCTGGCGGTCAACGGGTTCTACACCGCGCAGACCTCCCACTGGA
TCATGGTGATCCTGCTGTTCGTCGGCGGCTGCTCGCGCTCCATCCAGTTCACCTGCGTCAACGCCATCGCGTACGCCGAT
CTCGAATCCAAGGAGATGAGCGCGGCCACGAGCTTCGCCAGCGTCTGCCAGCAGCTGTCGCTGAGCCTCGGCGTCACGCT
GGGCGCGCTGGCGCTCGAGGGGACCGCCGGGCTCCACGGTCGAACGGAGATCCAGGCCTCGGATTTCGGGCCCGCCTTCT
TCGCCGTCGCCGTGATCTCGGCGGCCTCGGCACTGGCCTTCCGCAACCTGTCGCCGGATGCCGGCGCGGAGATCTCCGGG
CGGAAGATGGCTGCCGCGCCGCCCGCCCCGACGACCGAGATGAGGCCGCGGCACGGCTGA

Upstream 100 bases:

>100_bases
GGAAACTTAGCCATTGGAGCACCCGCGAAAAAGGTGGCTCCGCGCCGCTGCTCAACCTCTGTTGCACGATGGATACATTC
GCGAAACACGAGGGTGCGAG

Downstream 100 bases:

>100_bases
TCAGGCCGCGGCGCGGGCCGGCGGCCTGCCGGCAGCGCGCTTGCCGCCGTTCCGCGGCGCGCTGCCCTTGCCGGACGCCT
TCGCGGCGGCGGGCTTCGCG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MAALPCNRGGAPCVCCVMPATAQRPLWIVPLTVATALFMENTDSTVIATALPSIAASLHEDPIALKLALTSYLVSLAIFI
PVSGWMADRYGSRTVFRAALVVFMAGSLACAAANGLAWFVAARFLQGMGGAMMVPVGRLVVLRSVPKSQLVTALAYLTFP
ALVGPVLGPPVGGLITTWFDWRWIFFINLPIGCAGIVLASLYFEDVREPERPPLDLLGFVLLGTGLAALMLGLASLGRHL
LPAAVSWGCLAAGAVLLPLYWAHARRVAYPIVRLDLMQRPTFRVAVLGGSLFRIGTGAIPFLLPLMLQVGFGLDALQSGL
ITFAAAAGAMFVKTLGPRILRRTGFRALMVWNALLASAFLAVNGFYTAQTSHWIMVILLFVGGCSRSIQFTCVNAIAYAD
LESKEMSAATSFASVCQQLSLSLGVTLGALALEGTAGLHGRTEIQASDFGPAFFAVAVISAASALAFRNLSPDAGAEISG
RKMAAAPPAPTTEMRPRHG

Sequences:

>Translated_499_residues
MAALPCNRGGAPCVCCVMPATAQRPLWIVPLTVATALFMENTDSTVIATALPSIAASLHEDPIALKLALTSYLVSLAIFI
PVSGWMADRYGSRTVFRAALVVFMAGSLACAAANGLAWFVAARFLQGMGGAMMVPVGRLVVLRSVPKSQLVTALAYLTFP
ALVGPVLGPPVGGLITTWFDWRWIFFINLPIGCAGIVLASLYFEDVREPERPPLDLLGFVLLGTGLAALMLGLASLGRHL
LPAAVSWGCLAAGAVLLPLYWAHARRVAYPIVRLDLMQRPTFRVAVLGGSLFRIGTGAIPFLLPLMLQVGFGLDALQSGL
ITFAAAAGAMFVKTLGPRILRRTGFRALMVWNALLASAFLAVNGFYTAQTSHWIMVILLFVGGCSRSIQFTCVNAIAYAD
LESKEMSAATSFASVCQQLSLSLGVTLGALALEGTAGLHGRTEIQASDFGPAFFAVAVISAASALAFRNLSPDAGAEISG
RKMAAAPPAPTTEMRPRHG
>Mature_498_residues
AALPCNRGGAPCVCCVMPATAQRPLWIVPLTVATALFMENTDSTVIATALPSIAASLHEDPIALKLALTSYLVSLAIFIP
VSGWMADRYGSRTVFRAALVVFMAGSLACAAANGLAWFVAARFLQGMGGAMMVPVGRLVVLRSVPKSQLVTALAYLTFPA
LVGPVLGPPVGGLITTWFDWRWIFFINLPIGCAGIVLASLYFEDVREPERPPLDLLGFVLLGTGLAALMLGLASLGRHLL
PAAVSWGCLAAGAVLLPLYWAHARRVAYPIVRLDLMQRPTFRVAVLGGSLFRIGTGAIPFLLPLMLQVGFGLDALQSGLI
TFAAAAGAMFVKTLGPRILRRTGFRALMVWNALLASAFLAVNGFYTAQTSHWIMVILLFVGGCSRSIQFTCVNAIAYADL
ESKEMSAATSFASVCQQLSLSLGVTLGALALEGTAGLHGRTEIQASDFGPAFFAVAVISAASALAFRNLSPDAGAEISGR
KMAAAPPAPTTEMRPRHG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=476, Percent_Identity=37.8151260504202, Blast_Score=335, Evalue=5e-93,
Organism=Escherichia coli, GI1788392, Length=479, Percent_Identity=36.7432150313152, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1789042, Length=438, Percent_Identity=27.6255707762557, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1788710, Length=471, Percent_Identity=25.0530785562633, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87081983, Length=296, Percent_Identity=24.3243243243243, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1790146, Length=162, Percent_Identity=29.6296296296296, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=258, Percent_Identity=25.968992248062, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=28, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52552; Mature: 52421

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALPCNRGGAPCVCCVMPATAQRPLWIVPLTVATALFMENTDSTVIATALPSIAASLHE
CCCCCCCCCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCC
DPIALKLALTSYLVSLAIFIPVSGWMADRYGSRTVFRAALVVFMAGSLACAAANGLAWFV
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCHHHHHH
AARFLQGMGGAMMVPVGRLVVLRSVPKSQLVTALAYLTFPALVGPVLGPPVGGLITTWFD
HHHHHHCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHC
WRWIFFINLPIGCAGIVLASLYFEDVREPERPPLDLLGFVLLGTGLAALMLGLASLGRHL
EEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPAAVSWGCLAAGAVLLPLYWAHARRVAYPIVRLDLMQRPTFRVAVLGGSLFRIGTGAIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCHH
FLLPLMLQVGFGLDALQSGLITFAAAAGAMFVKTLGPRILRRTGFRALMVWNALLASAFL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHH
AVNGFYTAQTSHWIMVILLFVGGCSRSIQFTCVNAIAYADLESKEMSAATSFASVCQQLS
HHCCEEECCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHH
LSLGVTLGALALEGTAGLHGRTEIQASDFGPAFFAVAVISAASALAFRNLSPDAGAEISG
HHHCHHHHHHEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RKMAAAPPAPTTEMRPRHG
CEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
AALPCNRGGAPCVCCVMPATAQRPLWIVPLTVATALFMENTDSTVIATALPSIAASLHE
CCCCCCCCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCC
DPIALKLALTSYLVSLAIFIPVSGWMADRYGSRTVFRAALVVFMAGSLACAAANGLAWFV
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCHHHHHH
AARFLQGMGGAMMVPVGRLVVLRSVPKSQLVTALAYLTFPALVGPVLGPPVGGLITTWFD
HHHHHHCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHC
WRWIFFINLPIGCAGIVLASLYFEDVREPERPPLDLLGFVLLGTGLAALMLGLASLGRHL
EEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPAAVSWGCLAAGAVLLPLYWAHARRVAYPIVRLDLMQRPTFRVAVLGGSLFRIGTGAIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCHH
FLLPLMLQVGFGLDALQSGLITFAAAAGAMFVKTLGPRILRRTGFRALMVWNALLASAFL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHH
AVNGFYTAQTSHWIMVILLFVGGCSRSIQFTCVNAIAYADLESKEMSAATSFASVCQQLS
HHCCEEECCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHH
LSLGVTLGALALEGTAGLHGRTEIQASDFGPAFFAVAVISAASALAFRNLSPDAGAEISG
HHHCHHHHHHEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RKMAAAPPAPTTEMRPRHG
CEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]