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The map label for this gene is mltB [H]

Identifier: 170746489

GI number: 170746489

Start: 45919

End: 47109

Strand: Reverse

Name: mltB [H]

Synonym: Mrad2831_0039

Alternate gene names: 170746489

Gene position: 47109-45919 (Counterclockwise)

Preceding gene: 170746490

Following gene: 170746488

Centisome position: 0.78

GC content: 73.8

Gene sequence:

>1191_bases
ATGCTCTCCCGCCGATGGTTTCTGGCCGCGGCGGCGTCCCTCGCCACCGGGGAGGCCCGCGCCGAGGACGCCTTCCGGGC
GTTCATCGAGGCCCTGCGTCCGGACGCGGCGGCGCGGGGTGTCACCGCGGCGACTTTCGACGCGGCGTTTCGCGGGATCA
CGGGACCCGATGCCGGCATTCTGGCGCGCACCCGCCGCCAGAGCGAGTTCGTCCGGCCGGTCTGGGAGTACCTCGTCGGC
GCGGTCTCGGCCGGCCGCATCGCGCGCGGACGCGCCCGTGCGGCGGCCCTCGCGTCGACCCTGACGGCAATCGAGGGCCG
CTACGGCGTTCCGGGGCGGATGGCGATGGCGTTCTGGGGCGTCGAATCGGACTTCGGAGCGAGCGCGGGCAGCGTTCCGA
CGGTCCGGGCGCTGGCGACGCTCGCCGAGGCGCGGCACCGCGGCGGCCTGTTCCGCGACGAGCTCCTGGCCGCGCTCACG
ATCCTGCAGCGCGGCGACATCGCGCCGGACCGGATGAGCGGCAGCTGGGCCGGCGCGATGGGTCAGGTGCAGTTCCTGCC
CTCAACCTACCTGGCCCACGCGGTCGACTTCGACGGCGACGGACGCCGCGACATCTGGACCAGCGAGCCGGACTCGCTGG
CATCGATCGCGGCCTACCTCAAGGATCTCGGCTGGAATCCGGCGCTCTCTTGGGGCTACGAGGTGCTCCTGCCGAAGGAC
TTCGACCTGAACCGCTACGCGGGCGATCTCGGCAGCTTCGCCGAGCGAGGCGTTCGCCGCACCGACGGGAAGCCGCTGCC
GGGGACCGGACGTGCCAGCCTGTTCCTGCCCGGTGGCCTGGGGGCGCCGGTCTTCCTGATCACCGACAATTTCGAGGTGA
TCCGAGGCTACAACACCTCGGATTCCTACGCGCTGGCGGTGGGACACCTCGCAGACCGACTCGCCGGCGGTCCGCCGCTG
GCCGCGCCTTGGCCAGCCGCCGGCGCCCGCCTCGACGGGCCGGGCCTCCGCGCCCTGCAAACCGGCCTCGCGGCGGCCGG
CTTCTACGACGGGCCGACGGATGGACGGGCCGGCCCGAAGCTGCGCGAGGCGGTGCGGCGCTACCAGATCAGCGCCGGAT
TGCCGGCCGACGGCTACGCCACGCCGGCCCTGCTCGACCGCGTCACCGGCAAGGCGGCACCGCGACGCTGA

Upstream 100 bases:

>100_bases
CGCCTGGGGTGGCAGCCCCGGCACGACGACCTCGACGCCATCGTCGACCAGGCGCTCGCCTGGGAACAGCGCCTGGAGAA
GCGCAATCGGATCTGACCGG

Downstream 100 bases:

>100_bases
GCCCGTCGAGAGGTTTAGACCCGCGTGCTGCTTCCGCCTATAGTGGCCGCGTTCCGAACGGGTCCGGTTCGGCGTGAAGC
GTACGGAATGCGCATGGTCC

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MLSRRWFLAAAASLATGEARAEDAFRAFIEALRPDAAARGVTAATFDAAFRGITGPDAGILARTRRQSEFVRPVWEYLVG
AVSAGRIARGRARAAALASTLTAIEGRYGVPGRMAMAFWGVESDFGASAGSVPTVRALATLAEARHRGGLFRDELLAALT
ILQRGDIAPDRMSGSWAGAMGQVQFLPSTYLAHAVDFDGDGRRDIWTSEPDSLASIAAYLKDLGWNPALSWGYEVLLPKD
FDLNRYAGDLGSFAERGVRRTDGKPLPGTGRASLFLPGGLGAPVFLITDNFEVIRGYNTSDSYALAVGHLADRLAGGPPL
AAPWPAAGARLDGPGLRALQTGLAAAGFYDGPTDGRAGPKLREAVRRYQISAGLPADGYATPALLDRVTGKAAPRR

Sequences:

>Translated_396_residues
MLSRRWFLAAAASLATGEARAEDAFRAFIEALRPDAAARGVTAATFDAAFRGITGPDAGILARTRRQSEFVRPVWEYLVG
AVSAGRIARGRARAAALASTLTAIEGRYGVPGRMAMAFWGVESDFGASAGSVPTVRALATLAEARHRGGLFRDELLAALT
ILQRGDIAPDRMSGSWAGAMGQVQFLPSTYLAHAVDFDGDGRRDIWTSEPDSLASIAAYLKDLGWNPALSWGYEVLLPKD
FDLNRYAGDLGSFAERGVRRTDGKPLPGTGRASLFLPGGLGAPVFLITDNFEVIRGYNTSDSYALAVGHLADRLAGGPPL
AAPWPAAGARLDGPGLRALQTGLAAAGFYDGPTDGRAGPKLREAVRRYQISAGLPADGYATPALLDRVTGKAAPRR
>Mature_396_residues
MLSRRWFLAAAASLATGEARAEDAFRAFIEALRPDAAARGVTAATFDAAFRGITGPDAGILARTRRQSEFVRPVWEYLVG
AVSAGRIARGRARAAALASTLTAIEGRYGVPGRMAMAFWGVESDFGASAGSVPTVRALATLAEARHRGGLFRDELLAALT
ILQRGDIAPDRMSGSWAGAMGQVQFLPSTYLAHAVDFDGDGRRDIWTSEPDSLASIAAYLKDLGWNPALSWGYEVLLPKD
FDLNRYAGDLGSFAERGVRRTDGKPLPGTGRASLFLPGGLGAPVFLITDNFEVIRGYNTSDSYALAVGHLADRLAGGPPL
AAPWPAAGARLDGPGLRALQTGLAAAGFYDGPTDGRAGPKLREAVRRYQISAGLPADGYATPALLDRVTGKAAPRR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=215, Percent_Identity=34.8837209302326, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 41788; Mature: 41788

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRRWFLAAAASLATGEARAEDAFRAFIEALRPDAAARGVTAATFDAAFRGITGPDAGI
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHCCCCCCCCHH
LARTRRQSEFVRPVWEYLVGAVSAGRIARGRARAAALASTLTAIEGRYGVPGRMAMAFWG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEC
VESDFGASAGSVPTVRALATLAEARHRGGLFRDELLAALTILQRGDIAPDRMSGSWAGAM
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCC
GQVQFLPSTYLAHAVDFDGDGRRDIWTSEPDSLASIAAYLKDLGWNPALSWGYEVLLPKD
CEEEECCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
FDLNRYAGDLGSFAERGVRRTDGKPLPGTGRASLFLPGGLGAPVFLITDNFEVIRGYNTS
CCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCEEEEECCCCCCCEEEEECCEEEEECCCCC
DSYALAVGHLADRLAGGPPLAAPWPAAGARLDGPGLRALQTGLAAAGFYDGPTDGRAGPK
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHH
LREAVRRYQISAGLPADGYATPALLDRVTGKAAPRR
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLSRRWFLAAAASLATGEARAEDAFRAFIEALRPDAAARGVTAATFDAAFRGITGPDAGI
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHCCCCCCCCHH
LARTRRQSEFVRPVWEYLVGAVSAGRIARGRARAAALASTLTAIEGRYGVPGRMAMAFWG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEC
VESDFGASAGSVPTVRALATLAEARHRGGLFRDELLAALTILQRGDIAPDRMSGSWAGAM
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHCCC
GQVQFLPSTYLAHAVDFDGDGRRDIWTSEPDSLASIAAYLKDLGWNPALSWGYEVLLPKD
CEEEECCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
FDLNRYAGDLGSFAERGVRRTDGKPLPGTGRASLFLPGGLGAPVFLITDNFEVIRGYNTS
CCCHHHHHHHHHHHHCCCHHCCCCCCCCCCCEEEEECCCCCCCEEEEECCEEEEECCCCC
DSYALAVGHLADRLAGGPPLAAPWPAAGARLDGPGLRALQTGLAAAGFYDGPTDGRAGPK
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHH
LREAVRRYQISAGLPADGYATPALLDRVTGKAAPRR
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]