Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 170746461

GI number: 170746461

Start: 11528

End: 13960

Strand: Reverse

Name: ligA

Synonym: Mrad2831_0011

Alternate gene names: 170746461

Gene position: 13960-11528 (Counterclockwise)

Preceding gene: 170746462

Following gene: 170746460

Centisome position: 0.23

GC content: 71.97

Gene sequence:

>2433_bases
ATGGCCGCAACGAAATCCGAACCGCTCGATCTCGACCTCACCGCCGAGGAAGCGCGCGCCGAGTACGCGCGCCTGTCGGA
CGCGATCCTGGAGGCCGATCGCCTCTACTATCAGGAAGACGCGCCCGAGATATCGGACGCCGAGTACGACCGGCTGCGCC
GCCGCCTGGAAGACATCGAGGCCCGCTTCCCCGATCTCGCCGGTACCGGCGCCGCCAGCACGTCGGTCGGCGCGAAGCCT
TCGGAGAAGTTCGCCAAGGTGCGGCACGCGGTGCCCATGCTCTCGCTGGGCAACGCCTTCGACGACGCGGAGGTGGCCGA
GTTCGTCGCGCGCGTGCGCCGCTTCCTCGGCTGGTCGGACGACACTCCCCTCGCCTTCACGGCCGAGCCGAAGATCGACG
GCCTGTCCCTATCGCTCCGTTACGTGAACGGCCGGCTCGACACCGCCGCCACCCGCGGCGACGGCGAGGTCGGCGAGAAC
GTCACGGCCAATGCCCGCACGGTGCAGGACATCCCGGCGGTGCTCGCGGGGGCCGGCTGGCCGGAGATCTGCGAGGTGCG
CGGGGAGGTCTACCTGTCCCACGCCGACTTCGCCGCCATCAACGCCCGGCAGGAAGCGGCCGGCAAGCCTCTCTTCGCCA
ACCCGCGCAACGCCGCCGCCGGCAGCCTGCGCCAGCTCGATCCCGCGATCACCGCGTCCCGGCCGCTGCGATTCTTCGCC
TATGCCTGGGGCGAGCTGTCGGCGCCGATCGCCGAGACCCAGACCGGCGTGCTGGAGCAGTTCGCGACCTGGGGCCTGCC
GGTGAACCCGCGCACCGAGACCTTCACCGACACGGCGGCGATGCTCGCCCATTACCGCCGCATCGAGGCGGACCGGGCCG
GGCTCGGCTACGACATCGACGGCGTCGTCTACAAGGTCGACGACCTCGCGCTCCAGAAGCGGCTGGGCTTCGTCTCGCGC
TCGCCCCGCTGGGCGCTCGCCCACAAATTCGCCGCCCAGGAAGCCACGACGGTCGTCGAGGACATCGTCATCAATGTCGG
CCGCACCGGCTCGCTGAACCCGCTGGCCAAGCTCCGTCCCGTGACGGTGGGCGGCGTCGTCGTGTCGAACGCCACCCTGC
ACAACGAGGGCTACGTGAAGGGCGTCGGCGCCGACGGCGAGCCGATCCGCGACGGCCGCGACATCCGCGTCGGCGACACG
GTGACGGTGGTCCGGGCCGGCGACGTTATCCCCAAGGTCATGGACGTCGACCTCACGAAGCGCCCGGCCGACTCGCGGCC
CTACGCGTTCCCGGAGTCATGCCCGGCCTGCGGCAGCCGAGCCGTCCGCGCCATCAACCCGCGCACCGGCCGGCCCGACG
CGGTGCGCCGCTGCACCGGCGGCCTGATCTGCCCGGCGCAGGGCGTCGAGCGGATGAAGCACTTCGTGTCGCGCAACGGC
TTCGACATCGAGGGCTTCGGCGAGACGTATATCGAGGTCCTGTTCGAGGCCGGACTGGTGCGCCAGCCCGCCGACCTGTT
CCGCCTGGACTTCGAGGAACTCAAGGCCGCGATCGTGGCGCGGCGCGAGGCGCTGTCGGCCGAGCGGCGCGCGGAATCGG
GGACGGCCGAGCCGCCCAAGAAGGCCGCCAAGAAGAAGGGCGACGAGGACGACAAGGCGATCAAGAACCTGCTCGCCGCC
GTCGAGGCACGGCGCGCGGTGCCGATGAACCGCCTGCTCTTCGCCCTCGGTATCCCGCAGATCGGCGAGGCGACCGCCAA
GGCGCTGGCGAAGCGCTTCGCCGACATGCCGACGCTGATCGCGGCGATCCGCGAGGCCGCGGCGGCGCAGCCGGGTCCCG
ACTGGGTCGAGCTCACCGCAGTGCCGCGCGTCGGGGGCACCACCCGGGACCGGCTCCTCGATCTCGGCTTCCCGGAGGAC
GACGCGGCGCCGGCCAAGGAGCGGGCGCGCCTGAGCGCCGTCCAGCGCGAGAACCTCCTCGCCCATTACGGCGACGTCGA
CGCGGTGCGCGCCGCGATCGCGCAGGCTGCGGCGCAGCGTCCCGGCGATGCCTACCGGGTCTTCGCCGACGACGGCGAGA
TCGGCCCGGTGGCGACCGATGCCCTGCTCCTGTTCTTCTCCGAGCCGCACAACGCCGACGCCGTCACCGCGCTGCTCGAC
GCGGTCGCGGTGCAGCCGATGGAGCGGGCCGCCGCGGCCTCCACCTTCGCGGGGAAGACCGTGGTCTTCACCGGCGCCCT
GGAGAAGATGACCCGCAACGAGGCCAAGGCGGTGGCCGAGCGGCTGGGCGCGAAGGTCTCGGGCTCGGTCTCGGCCAAGA
CCGACCTGGTCGTCGCCGGCCCGGGAGCGGGCTCGAAGCTGAAGGACGCCCAGAAGCACGGTGTCCGGGTCGTCTCGGAG
GACGATTGGCTGGCGATGGTGGCGCAGGGTTGA

Upstream 100 bases:

>100_bases
GCGAGGCGCGGCGATCTGCTCAGCCCCCGCCCGTGAGGGAATCGGGGACTGCGCGCCGCGGCCGGCTCCCCGCCGGCCGC
CGATGCGTTAGAACCCCGGC

Downstream 100 bases:

>100_bases
AGCCCGAGTCCGGCCGGCCTAGACCTCTCGGGGAACACCCCGAGAACTTCCACCCGGTATGGTTGAACCGCGATGACGGT
CCTGGCCCTCGACTTCGAGA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MAATKSEPLDLDLTAEEARAEYARLSDAILEADRLYYQEDAPEISDAEYDRLRRRLEDIEARFPDLAGTGAASTSVGAKP
SEKFAKVRHAVPMLSLGNAFDDAEVAEFVARVRRFLGWSDDTPLAFTAEPKIDGLSLSLRYVNGRLDTAATRGDGEVGEN
VTANARTVQDIPAVLAGAGWPEICEVRGEVYLSHADFAAINARQEAAGKPLFANPRNAAAGSLRQLDPAITASRPLRFFA
YAWGELSAPIAETQTGVLEQFATWGLPVNPRTETFTDTAAMLAHYRRIEADRAGLGYDIDGVVYKVDDLALQKRLGFVSR
SPRWALAHKFAAQEATTVVEDIVINVGRTGSLNPLAKLRPVTVGGVVVSNATLHNEGYVKGVGADGEPIRDGRDIRVGDT
VTVVRAGDVIPKVMDVDLTKRPADSRPYAFPESCPACGSRAVRAINPRTGRPDAVRRCTGGLICPAQGVERMKHFVSRNG
FDIEGFGETYIEVLFEAGLVRQPADLFRLDFEELKAAIVARREALSAERRAESGTAEPPKKAAKKKGDEDDKAIKNLLAA
VEARRAVPMNRLLFALGIPQIGEATAKALAKRFADMPTLIAAIREAAAAQPGPDWVELTAVPRVGGTTRDRLLDLGFPED
DAAPAKERARLSAVQRENLLAHYGDVDAVRAAIAQAAAQRPGDAYRVFADDGEIGPVATDALLLFFSEPHNADAVTALLD
AVAVQPMERAAAASTFAGKTVVFTGALEKMTRNEAKAVAERLGAKVSGSVSAKTDLVVAGPGAGSKLKDAQKHGVRVVSE
DDWLAMVAQG

Sequences:

>Translated_810_residues
MAATKSEPLDLDLTAEEARAEYARLSDAILEADRLYYQEDAPEISDAEYDRLRRRLEDIEARFPDLAGTGAASTSVGAKP
SEKFAKVRHAVPMLSLGNAFDDAEVAEFVARVRRFLGWSDDTPLAFTAEPKIDGLSLSLRYVNGRLDTAATRGDGEVGEN
VTANARTVQDIPAVLAGAGWPEICEVRGEVYLSHADFAAINARQEAAGKPLFANPRNAAAGSLRQLDPAITASRPLRFFA
YAWGELSAPIAETQTGVLEQFATWGLPVNPRTETFTDTAAMLAHYRRIEADRAGLGYDIDGVVYKVDDLALQKRLGFVSR
SPRWALAHKFAAQEATTVVEDIVINVGRTGSLNPLAKLRPVTVGGVVVSNATLHNEGYVKGVGADGEPIRDGRDIRVGDT
VTVVRAGDVIPKVMDVDLTKRPADSRPYAFPESCPACGSRAVRAINPRTGRPDAVRRCTGGLICPAQGVERMKHFVSRNG
FDIEGFGETYIEVLFEAGLVRQPADLFRLDFEELKAAIVARREALSAERRAESGTAEPPKKAAKKKGDEDDKAIKNLLAA
VEARRAVPMNRLLFALGIPQIGEATAKALAKRFADMPTLIAAIREAAAAQPGPDWVELTAVPRVGGTTRDRLLDLGFPED
DAAPAKERARLSAVQRENLLAHYGDVDAVRAAIAQAAAQRPGDAYRVFADDGEIGPVATDALLLFFSEPHNADAVTALLD
AVAVQPMERAAAASTFAGKTVVFTGALEKMTRNEAKAVAERLGAKVSGSVSAKTDLVVAGPGAGSKLKDAQKHGVRVVSE
DDWLAMVAQG
>Mature_809_residues
AATKSEPLDLDLTAEEARAEYARLSDAILEADRLYYQEDAPEISDAEYDRLRRRLEDIEARFPDLAGTGAASTSVGAKPS
EKFAKVRHAVPMLSLGNAFDDAEVAEFVARVRRFLGWSDDTPLAFTAEPKIDGLSLSLRYVNGRLDTAATRGDGEVGENV
TANARTVQDIPAVLAGAGWPEICEVRGEVYLSHADFAAINARQEAAGKPLFANPRNAAAGSLRQLDPAITASRPLRFFAY
AWGELSAPIAETQTGVLEQFATWGLPVNPRTETFTDTAAMLAHYRRIEADRAGLGYDIDGVVYKVDDLALQKRLGFVSRS
PRWALAHKFAAQEATTVVEDIVINVGRTGSLNPLAKLRPVTVGGVVVSNATLHNEGYVKGVGADGEPIRDGRDIRVGDTV
TVVRAGDVIPKVMDVDLTKRPADSRPYAFPESCPACGSRAVRAINPRTGRPDAVRRCTGGLICPAQGVERMKHFVSRNGF
DIEGFGETYIEVLFEAGLVRQPADLFRLDFEELKAAIVARREALSAERRAESGTAEPPKKAAKKKGDEDDKAIKNLLAAV
EARRAVPMNRLLFALGIPQIGEATAKALAKRFADMPTLIAAIREAAAAQPGPDWVELTAVPRVGGTTRDRLLDLGFPEDD
AAPAKERARLSAVQRENLLAHYGDVDAVRAAIAQAAAQRPGDAYRVFADDGEIGPVATDALLLFFSEPHNADAVTALLDA
VAVQPMERAAAASTFAGKTVVFTGALEKMTRNEAKAVAERLGAKVSGSVSAKTDLVVAGPGAGSKLKDAQKHGVRVVSED
DWLAMVAQG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=611, Percent_Identity=44.189852700491, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI87082305, Length=500, Percent_Identity=23, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_METRJ (B1M575)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001752721.1
- ProteinModelPortal:   B1M575
- GeneID:   6136309
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_0011
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK2332283
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 86773; Mature: 86642

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 131-131 BINDING 129-129 BINDING 152-152 BINDING 189-189 BINDING 305-305 BINDING 329-329

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATKSEPLDLDLTAEEARAEYARLSDAILEADRLYYQEDAPEISDAEYDRLRRRLEDIE
CCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
ARFPDLAGTGAASTSVGAKPSEKFAKVRHAVPMLSLGNAFDDAEVAEFVARVRRFLGWSD
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHCCCC
DTPLAFTAEPKIDGLSLSLRYVNGRLDTAATRGDGEVGENVTANARTVQDIPAVLAGAGW
CCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PEICEVRGEVYLSHADFAAINARQEAAGKPLFANPRNAAAGSLRQLDPAITASRPLRFFA
HHHHHCCCCEEEECCCHHHHCCHHHHCCCCCCCCCCCCHHCCHHHCCCHHHCCCCHHEEH
YAWGELSAPIAETQTGVLEQFATWGLPVNPRTETFTDTAAMLAHYRRIEADRAGLGYDID
HHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
GVVYKVDDLALQKRLGFVSRSPRWALAHKFAAQEATTVVEDIVINVGRTGSLNPLAKLRP
CEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC
VTVGGVVVSNATLHNEGYVKGVGADGEPIRDGRDIRVGDTVTVVRAGDVIPKVMDVDLTK
EEECCEEEECCEECCCCEEEEECCCCCCCCCCCCEEECCEEEEEECCCCCHHHHCCCCCC
RPADSRPYAFPESCPACGSRAVRAINPRTGRPDAVRRCTGGLICPAQGVERMKHFVSRNG
CCCCCCCCCCCCCCCCHHHHCEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCC
FDIEGFGETYIEVLFEAGLVRQPADLFRLDFEELKAAIVARREALSAERRAESGTAEPPK
CCCCCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
KAAKKKGDEDDKAIKNLLAAVEARRAVPMNRLLFALGIPQIGEATAKALAKRFADMPTLI
HHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHH
AAIREAAAAQPGPDWVELTAVPRVGGTTRDRLLDLGFPEDDAAPAKERARLSAVQRENLL
HHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHH
AHYGDVDAVRAAIAQAAAQRPGDAYRVFADDGEIGPVATDALLLFFSEPHNADAVTALLD
HHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCEEEEEECCCCCCHHHHHHHH
AVAVQPMERAAAASTFAGKTVVFTGALEKMTRNEAKAVAERLGAKVSGSVSAKTDLVVAG
HHHHCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
PGAGSKLKDAQKHGVRVVSEDDWLAMVAQG
CCCCCHHHHHHHCCCEEEECCCEEEEEECC
>Mature Secondary Structure 
AATKSEPLDLDLTAEEARAEYARLSDAILEADRLYYQEDAPEISDAEYDRLRRRLEDIE
CCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
ARFPDLAGTGAASTSVGAKPSEKFAKVRHAVPMLSLGNAFDDAEVAEFVARVRRFLGWSD
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHCCCC
DTPLAFTAEPKIDGLSLSLRYVNGRLDTAATRGDGEVGENVTANARTVQDIPAVLAGAGW
CCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PEICEVRGEVYLSHADFAAINARQEAAGKPLFANPRNAAAGSLRQLDPAITASRPLRFFA
HHHHHCCCCEEEECCCHHHHCCHHHHCCCCCCCCCCCCHHCCHHHCCCHHHCCCCHHEEH
YAWGELSAPIAETQTGVLEQFATWGLPVNPRTETFTDTAAMLAHYRRIEADRAGLGYDID
HHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
GVVYKVDDLALQKRLGFVSRSPRWALAHKFAAQEATTVVEDIVINVGRTGSLNPLAKLRP
CEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCC
VTVGGVVVSNATLHNEGYVKGVGADGEPIRDGRDIRVGDTVTVVRAGDVIPKVMDVDLTK
EEECCEEEECCEECCCCEEEEECCCCCCCCCCCCEEECCEEEEEECCCCCHHHHCCCCCC
RPADSRPYAFPESCPACGSRAVRAINPRTGRPDAVRRCTGGLICPAQGVERMKHFVSRNG
CCCCCCCCCCCCCCCCHHHHCEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCC
FDIEGFGETYIEVLFEAGLVRQPADLFRLDFEELKAAIVARREALSAERRAESGTAEPPK
CCCCCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
KAAKKKGDEDDKAIKNLLAAVEARRAVPMNRLLFALGIPQIGEATAKALAKRFADMPTLI
HHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHH
AAIREAAAAQPGPDWVELTAVPRVGGTTRDRLLDLGFPEDDAAPAKERARLSAVQRENLL
HHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHH
AHYGDVDAVRAAIAQAAAQRPGDAYRVFADDGEIGPVATDALLLFFSEPHNADAVTALLD
HHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCEEEEEECCCCCCHHHHHHHH
AVAVQPMERAAAASTFAGKTVVFTGALEKMTRNEAKAVAERLGAKVSGSVSAKTDLVVAG
HHHHCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
PGAGSKLKDAQKHGVRVVSEDDWLAMVAQG
CCCCCHHHHHHHCCCEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA