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The map label for this gene is insD1 [C]

Identifier: 170684099

GI number: 170684099

Start: 1662987

End: 1663892

Strand: Reverse

Name: insD1 [C]

Synonym: EcSMS35_1668

Alternate gene names: 170684099

Gene position: 1663892-1662987 (Counterclockwise)

Preceding gene: 170683416

Following gene: 170679722

Centisome position: 32.83

GC content: 54.97

Gene sequence:

>906_bases
GTGGATAGCGCACGCGCCCTTATTGCCCGGGGATGGGGAGTAAGCTTAGTCAGCCGTTGTCTCCGGGTGTCGCGTGCGCA
GTTGCACGTAATTCTCAGACGAACCGATGACTGGAAAGATGGTCGCCGCAGCCGTCACTCAGATGATACGGATGTGCTTC
TCCGTATACACCATGTTATCGGAGAGCTGCCCACGTATGGTTATCGTCGGGTATGGGCGCTGCTTCGCAGACAGGCCGAA
CTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGTTGCTTGAGCGAAAAAC
CGCTGTACCGCCATCGAAACGGGCACATACTGGCAAAGTGGCCGTGAAAGAAAGCAATCAACGATGGTGCTCTGACGGGT
TCGAGTTCCGCTGTGATAACGGAGAAAAACTGCGAGTCACGTTCGCGCTGGACTGCTGTGACCGTGAGGCACTGCACTGG
GCAGTCACTACGGGCGGCTTCGACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGA
GCTTCCGGCGTCTCCAGTAGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGGCAGTTTGCCCGGA
TGTTGGGGCTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGCATAGCAGAGAGCTTCGTGAAAACGATA
AAGCGTGACTACATCAGTATCATGCCTAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTA
TAACGAATGGCATCCGCATAGTGCACTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCAGGCCAGTAATGGGTTAA
GTGATAACAGGTGTCTGGAAATATAG

Upstream 100 bases:

>100_bases
CTGCCATGAAGCAGATTAAAGAACTCCAGCGCCTGCTCGGCAAAAAAACGATGGAAAATGAACTCCTTAAAGAAGCCGTT
GAATACGGGCGTGCAAAAAA

Downstream 100 bases:

>100_bases
GGGCAAATCCAAGCGCCTCGATCGTTCCCTGGCTTGCGTTTCCATTCACCGTGATAGCTCTTGCCTTAAGTGGAAACTGT
GCATTACGAGTGATCTCATC

Product: IS2 transposase orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAE
LDGMPAINAKRVYRIMRQNALLLERKTAVPPSKRAHTGKVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHW
AVTTGGFDSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTI
KRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQQASNGLSDNRCLEI

Sequences:

>Translated_301_residues
MDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAE
LDGMPAINAKRVYRIMRQNALLLERKTAVPPSKRAHTGKVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHW
AVTTGGFDSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTI
KRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQQASNGLSDNRCLEI
>Mature_301_residues
MDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAE
LDGMPAINAKRVYRIMRQNALLLERKTAVPPSKRAHTGKVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHW
AVTTGGFDSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTI
KRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQQASNGLSDNRCLEI

Specific function: Involved in the transposition of the insertion sequence is2 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790724, Length=301, Percent_Identity=96.3455149501661, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI1789424, Length=301, Percent_Identity=96.3455149501661, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI1789224, Length=301, Percent_Identity=96.3455149501661, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI1788305, Length=301, Percent_Identity=96.3455149501661, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI1787669, Length=301, Percent_Identity=96.3455149501661, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI1786558, Length=301, Percent_Identity=96.3455149501661, Blast_Score=604, Evalue=1e-174,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR002514 [H]

Pfam domain/function: PF00665 rve; PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 34337; Mature: 34337

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHSDDTDVLLRIHHVI
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHCCCCCCHHHHHHHHHHH
GELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKTAVPPSKRAHTGKV
HCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
AVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFDSETVQDVMLGAV
EEECCCCHHHCCCCEEEECCCCEEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHH
ERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTI
HHHHCCCCCCCHHHHHCCCCCEEECHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
KRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQQASNGLSDNRCLE
HHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEC
I
C
>Mature Secondary Structure
MDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHSDDTDVLLRIHHVI
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHHCCCCCCHHHHHHHHHHH
GELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKTAVPPSKRAHTGKV
HCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
AVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFDSETVQDVMLGAV
EEECCCCHHHCCCCEEEECCCCEEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHH
ERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTI
HHHHCCCCCCCHHHHHCCCCCEEECHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
KRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQQASNGLSDNRCLE
HHHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA