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The map label for this gene is cho [H]

Identifier: 170683911

GI number: 170683911

Start: 1434697

End: 1435584

Strand: Reverse

Name: cho [H]

Synonym: EcSMS35_1449

Alternate gene names: 170683911

Gene position: 1435584-1434697 (Counterclockwise)

Preceding gene: 170680478

Following gene: 170680684

Centisome position: 28.32

GC content: 50.79

Gene sequence:

>888_bases
GTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCGAACATTTACGTTCATT
CCTTAATGACTTACCCACCCGACCAGGGGTGTATCTGTTTCATGGCGAAAGTGACACCATGCCGCTCTATATCGGCAAAA
GCATTAACATCCGCAGTCGGGTGCTTTCTCATTTACGCACCCCTGATGAAGCCGCCATGCTACGGCAATCCCGACGCATC
AGCTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCACTGTTTAA
TAAACGCTTGCGGCGAAATCGCCAGCTCTGTGCCTTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGG
TGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCT
GATGAACAAAAACTTTGTTATGGTCTGTTGGGACTGGAGCCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAA
ACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGACGATCATGCGCTACGTTTGCGCCAGTCTCTGGAGCGTTTGC
GGGTGGTGTGTTGGCCTTGGCAAGGGGCGGTGGCGCTGAAAGAACAGCACCCGGAAATGACTCAATATCATATTATTCAA
AACTGGCTGTGGCTGGGGGCGGTTAATTCGCTGGAAGAAGCGACAATGTTAATTCGGACACCCGCCGGGTTTGATCACGA
CGGTTATAAAATTCTTTGTAAGCCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAG
CCAGTTGA

Upstream 100 bases:

>100_bases
GTAGCACGTCAGACGGTGTAACCTTTGTCATCGACCCGCCTCTTTTTTAATCGCTCTCCGCCTGTTACACTGGATAGATA
ACCAGCATTCGGAGTCAACA

Downstream 100 bases:

>100_bases
TTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCG
GTAGTGAGCAATTTATCGCC

Product: nucleotide excision repair endonuclease

Products: NA

Alternate protein names: Endonuclease cho; UvrC homolog protein [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGESDTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRI
SWICTAGEIGALLLEARLIKEQQPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCWPWQGAVALKEQHPEMTQYHIIQ
NWLWLGAVNSLEEATMLIRTPAGFDHDGYKILCKPLLSGNYEITELDPANDQRAS

Sequences:

>Translated_295_residues
MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGESDTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRI
SWICTAGEIGALLLEARLIKEQQPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCWPWQGAVALKEQHPEMTQYHIIQ
NWLWLGAVNSLEEATMLIRTPAGFDHDGYKILCKPLLSGNYEITELDPANDQRAS
>Mature_295_residues
MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGESDTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRI
SWICTAGEIGALLLEARLIKEQQPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCWPWQGAVALKEQHPEMTQYHIIQ
NWLWLGAVNSLEEATMLIRTPAGFDHDGYKILCKPLLSGNYEITELDPANDQRAS

Specific function: Incises the DNA at the 3' side of a lesion during nucleotide excision repair. Incises the DNA farther away from the lesion than uvrC. Not able to incise the 5' site of a lesion. When a lesion remains because uvrC is not able to induce the 3' incision, cho

COG id: COG0322

COG function: function code L; Nuclease subunit of the excinuclease complex

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrC C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788037, Length=295, Percent_Identity=98.6440677966102, Blast_Score=602, Evalue=1e-174,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000305 [H]

Pfam domain/function: PF01541 GIY-YIG [H]

EC number: NA

Molecular weight: Translated: 33755; Mature: 33755

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS50164 UVRC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGESDTMPLYIGKSINIRSR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCHHHH
VLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQQPLFNKRLRRNRQLCALQ
HHHHHCCCHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEE
LNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIADEQKLCYGLLGLEPLSRGRA
ECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
CFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCWPWQGAVALKEQHPEMTQYHIIQ
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHCCCCHHHHHHHH
NWLWLGAVNSLEEATMLIRTPAGFDHDGYKILCKPLLSGNYEITELDPANDQRAS
HHHHHHHHHHHHHHEEEEECCCCCCCCCHHEEEEHHHCCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGESDTMPLYIGKSINIRSR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCHHHH
VLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQQPLFNKRLRRNRQLCALQ
HHHHHCCCHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEE
LNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIADEQKLCYGLLGLEPLSRGRA
ECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
CFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCWPWQGAVALKEQHPEMTQYHIIQ
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCHHHHCCCCHHHHHHHH
NWLWLGAVNSLEEATMLIRTPAGFDHDGYKILCKPLLSGNYEITELDPANDQRAS
HHHHHHHHHHHHHHEEEEECCCCCCCCCHHEEEEHHHCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]