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The map label for this gene is ilvB [H]

Identifier: 170683518

GI number: 170683518

Start: 4127897

End: 4129585

Strand: Reverse

Name: ilvB [H]

Synonym: EcSMS35_4038

Alternate gene names: 170683518

Gene position: 4129585-4127897 (Counterclockwise)

Preceding gene: 170681558

Following gene: 170684308

Centisome position: 81.48

GC content: 56.25

Gene sequence:

>1689_bases
ATGGCAAGTTCGGGCACAACATCGACGCGTAAGCGCTTTACCGGCGCAGAATTTATCGTTCATTTCCTGGAACAGCAGGG
CATTAAGATTGTGACGGGCATTCCGGGCGGTTCTATCCTGCCTGTTTACGATGCCTTAAGCCAAAGCACGCAAATCCGCC
ATATTCTGGCTCGCCATGAACAGGGAGCGGGGTTTATCGCTCAGGGAATGGCGCGCACCGACGGTAAACCGGCGGTCTGT
ATGGCCTGTAGCGGACCGGGTGCGACTAACCTGGTGACCGCCATTGCCGATGCGCGGCTGGATTCCATCCCGCTGATTTG
CATCACCGGTCAGGTTCCTGCCTCGATGATCGGCACCGACGCCTTCCAGGAAGTGGACACCTACGGCATCTCTATCCCCA
TCACCAAACACAACTATCTGGTCAGACATATCGAAGAGCTCCCACAGGTCATGAGCGACGCCTTTCGTATTGCGCAATCA
GGCCGCCCTGGCCCGGTGTGGATAGACATTCCTAAGGATGTGCAAACGGCGGTTTTTGAGATTGAAGCACAGGCCGCTAT
GGCAGAAAAAGCTGCCGCGCCTGCGTTTAGTGAAGAGAGTATTCGTGACGCAGCTGCAATGATTAACGCCGCCAAACGCC
CGGTGCTTTATCTGGGCGGCGGTGTGATCAATGCGCCCGCACGGGTGCGTGAACTGGCAGAGAAAGCGCAATTGCCAACT
ACCATGACCTTAATGGCGCTGGGCATGCTGCCAAAAGCGCATCCGCTGTCGCTGGGTATGCTGGGGATGCACGGCGTGCG
CAGCACCAACTATATACTGCAGGAGGCGGATCTGCTGATTGTTCTCGGTGCGCGTTTTGATGACCGGGCGATTGGCAAAA
CCGAGCAGTTCTGCCCGAATGCGAAAATCATTCATGTCGATATCGACCGTGCAGAGCTGGGCAAAATCAAGCAACCGCAC
GTAGCGATTCAGGCGGATGTTGATGACGTGCTGGCGCAATTGATCCCGCAGGTGGAAGCGCAACCGCGTGCAGAGTGGCA
CCAGTTGGTGGCGGATTTGCAGCGCGAATTCCCGTATCCAATCCCGAAAGCGTGCGATCCGTTAACGCATTACGGCCTGA
TCAACGCTGTTGCCGCCTGTGTCGATGACAATGCGATTATCACCACCGACGTGGGTCAGCATCAGATGTGGACCGCGCAA
GCCTATCCGCTCAATCGCCCGCGCCAGTGGCTGACCTCCGGCGGGCTGGGCACGATGGGCTTTGGCCTGCCTGCGGCGAT
TGGCGCGGCGCTGGCGAACCCGGATCGCAAAGTGTTGTGTTTCTCCGGCGACGGCAGCCTGATGATGAATATTCAGGAGA
TGGCGACCGCCAGTGAAAATCAGCTGGATGTCAAAATCATTCTGATGAACAACGAAGCGCTGGGGCTGGTACATCAGCAA
CAGAGTCTGTTCTACAAGCAGGGCGTTTTTGCCGCTACCTATCCGGGAAAAATCAACTTTATGCAGATTGCCGCCGGATT
CGGCCTCGAAACCTGTGATTTGAATAACGAAACCGATCCGCAGGCTGCATTACAGGAAATCATCAATCGCCCTGGTCCGG
CGCTGATCCATGTGCGTATTGATGCCGAAGAAAAAGTGTACCCGATGGTGCCGCCAGGTGCGGCGAATACTGAAATGGTG
GGGGAATAA

Upstream 100 bases:

>100_bases
GGAACAACACACGATTCCAAAACCCCGCCGGCGCAAACCGGGCGGGGTTTTTCGTTTAAGCACCTCCCGGAAAGTCGGCC
CAGAAGAAAAGGACTGGAGC

Downstream 100 bases:

>100_bases
GCCATGCAAAACACAACTCATGACAACGTGATTCTGGAACTCACCGTTCGCAACCATCCGGGCGTAATGACCCACGTTTG
TGGCCTTTTTGCCCGTCGCG

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYDALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVC
MACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
GRPGPVWIDIPKDVQTAVFEIEAQAAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYLGGGVINAPARVRELAEKAQLPT
TMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPH
VAIQADVDDVLAQLIPQVEAQPRAEWHQLVADLQREFPYPIPKACDPLTHYGLINAVAACVDDNAIITTDVGQHQMWTAQ
AYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNEALGLVHQQ
QSLFYKQGVFAATYPGKINFMQIAAGFGLETCDLNNETDPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMV
GE

Sequences:

>Translated_562_residues
MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYDALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVC
MACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
GRPGPVWIDIPKDVQTAVFEIEAQAAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYLGGGVINAPARVRELAEKAQLPT
TMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPH
VAIQADVDDVLAQLIPQVEAQPRAEWHQLVADLQREFPYPIPKACDPLTHYGLINAVAACVDDNAIITTDVGQHQMWTAQ
AYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNEALGLVHQQ
QSLFYKQGVFAATYPGKINFMQIAAGFGLETCDLNNETDPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMV
GE
>Mature_561_residues
ASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYDALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCM
ACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQSG
RPGPVWIDIPKDVQTAVFEIEAQAAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYLGGGVINAPARVRELAEKAQLPTT
MTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHV
AIQADVDDVLAQLIPQVEAQPRAEWHQLVADLQREFPYPIPKACDPLTHYGLINAVAACVDDNAIITTDVGQHQMWTAQA
YPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNEALGLVHQQQ
SLFYKQGVFAATYPGKINFMQIAAGFGLETCDLNNETDPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVG
E

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=574, Percent_Identity=26.1324041811847, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI21361361, Length=557, Percent_Identity=25.3141831238779, Blast_Score=168, Evalue=1e-41,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=98.576512455516, Blast_Score=1139, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=565, Percent_Identity=39.646017699115, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1786717, Length=482, Percent_Identity=35.4771784232365, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1787096, Length=548, Percent_Identity=29.014598540146, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1788716, Length=477, Percent_Identity=26.8343815513627, Blast_Score=145, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=560, Percent_Identity=24.8214285714286, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17531301, Length=560, Percent_Identity=24.8214285714286, Blast_Score=151, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=591, Percent_Identity=26.3959390862944, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=575, Percent_Identity=40.695652173913, Blast_Score=437, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6323073, Length=483, Percent_Identity=21.3250517598344, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19922626, Length=477, Percent_Identity=29.3501048218029, Blast_Score=175, Evalue=9e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60487; Mature: 60356

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYDALSQSTQIRHILARHE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHCHHHHHHHHHHHCC
QGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTD
CCCCHHHHCHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCH
AFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQSGRPGPVWIDIPKDVQTAVFE
HHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHH
IEAQAAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYLGGGVINAPARVRELAEKAQLPT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCHHHHHHHHHHCCCH
TMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQFCPN
HHHHHHHHCCCCCCCCCEEHHCCCCCCCCCHHEECCCEEEEEECCCCCCCCCCHHHHCCC
AKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQLIPQVEAQPRAEWHQLVADLQREFPYP
CEEEEEECCHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
IPKACDPLTHYGLINAVAACVDDNAIITTDVGQHQMWTAQAYPLNRPRQWLTSGGLGTMG
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCHHHHHCCCCCCCC
FGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNEALGLVHQQ
CCHHHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHCCCCCCEEEEEEEECCCCEEEEEHH
QSLFYKQGVFAATYPGKINFMQIAAGFGLETCDLNNETDPQAALQEIINRPGPALIHVRI
HHHHHHCCCEEEECCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEE
DAEEKVYPMVPPGAANTEMVGE
CCCCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYDALSQSTQIRHILARHE
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHCHHHHHHHHHHHCC
QGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTD
CCCCHHHHCHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCH
AFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQSGRPGPVWIDIPKDVQTAVFE
HHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHH
IEAQAAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYLGGGVINAPARVRELAEKAQLPT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCHHHHHHHHHHCCCH
TMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEADLLIVLGARFDDRAIGKTEQFCPN
HHHHHHHHCCCCCCCCCEEHHCCCCCCCCCHHEECCCEEEEEECCCCCCCCCCHHHHCCC
AKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQLIPQVEAQPRAEWHQLVADLQREFPYP
CEEEEEECCHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
IPKACDPLTHYGLINAVAACVDDNAIITTDVGQHQMWTAQAYPLNRPRQWLTSGGLGTMG
CCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCHHHHHCCCCCCCC
FGLPAAIGAALANPDRKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNEALGLVHQQ
CCHHHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHCCCCCCEEEEEEEECCCCEEEEEHH
QSLFYKQGVFAATYPGKINFMQIAAGFGLETCDLNNETDPQAALQEIINRPGPALIHVRI
HHHHHHCCCEEEECCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEE
DAEEKVYPMVPPGAANTEMVGE
CCCCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]