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The map label for this gene is rstA [H]

Identifier: 170683236

GI number: 170683236

Start: 1579611

End: 1580330

Strand: Reverse

Name: rstA [H]

Synonym: EcSMS35_1591

Alternate gene names: 170683236

Gene position: 1580330-1579611 (Counterclockwise)

Preceding gene: 170683762

Following gene: 170682743

Centisome position: 31.18

GC content: 49.72

Gene sequence:

>720_bases
ATGAACACTATCGTATTTGTGGAAGATGATGCGGAAGTCGGTGCACTGATTGCCGCATACCTGGCAAAACATGATATGCA
GGTTACCGTAGAGCCGCGCGGCGACCGGGCCGAAGAAACTATTCTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCA
TGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCT
CTCGATAGCGATATGAACCACATCCTGGCACTGGAAATGGGCGCCTGCGACTATATTCTCAAAACGACGCCCCCTGCTGT
TTTGCTAGCGCGTTTACGTTTGCATTTGCGTCAGAATGAGCAAGCCACAGTGACCAAAGGTATTCAGGAAACGTCTCTGA
CTCCCTACAAAGCCCTGCATTTCGGCACGTTGACCATCGATCCCATCAACCGCGTAGTCACCCTGGCTAACACTGAAATC
TCGCTCTCGACAGCTGATTTCGAATTATTGTGGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCT
GAAAAATTTACGCGGCGTCAGTTATGACGGTCTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGC
TCGATAACGCCGCAGAACCTTATCGTATTAAAACCGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCGTGGGAATAA

Upstream 100 bases:

>100_bases
TCATCAGTAGAATACCTGATGAAACCTTGTTTAGAAACGATTGATAGTAAGTAAAAACAGTGCGGTGTATTGTGACGTTT
TTATATCTACCGTGAATGTT

Downstream 100 bases:

>100_bases
GCGATGAAAAAACTGTTTATCCAGTTTTACCTGTTATTGTTTGTCTGCTTCCTTGTGATGTCTCTGCTGGTCGGGCTGGT
GTACAAATTTACCGCCGAAC

Product: DNA-binding transcriptional regulator RstA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRENPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTS
LDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEI
SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWE

Sequences:

>Translated_239_residues
MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRENPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTS
LDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEI
SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWE
>Mature_239_residues
MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRENPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTS
LDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEI
SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWE

Specific function: Member of the two-component regulatory system rstB/rstA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693140, Length=239, Percent_Identity=98.326359832636, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=34.3347639484979, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI87082012, Length=230, Percent_Identity=32.6086956521739, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786784, Length=231, Percent_Identity=32.4675324675325, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=32.8947368421053, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI2367329, Length=233, Percent_Identity=32.1888412017167, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1786599, Length=234, Percent_Identity=29.9145299145299, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1786911, Length=234, Percent_Identity=29.0598290598291, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1790863, Length=190, Percent_Identity=33.6842105263158, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790860, Length=231, Percent_Identity=27.7056277056277, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787229, Length=232, Percent_Identity=29.7413793103448, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789402, Length=230, Percent_Identity=30.4347826086957, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1787375, Length=241, Percent_Identity=28.2157676348548, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790552, Length=233, Percent_Identity=28.755364806867, Blast_Score=71, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26702; Mature: 26702

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRENPDLVLLDIMLPGKDG
CCEEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCCC
MTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNE
HHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHCCCC
QATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEISLSTADFELLWELATHAGQI
CHHHHCCCHHCCCCCCCEEEEEEEEECCCCEEEEEECCEEEEECHHHHHHHHHHHHHHHH
MDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCC
>Mature Secondary Structure
MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRENPDLVLLDIMLPGKDG
CCEEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCCC
MTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKTTPPAVLLARLRLHLRQNE
HHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHCCCC
QATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEISLSTADFELLWELATHAGQI
CHHHHCCCHHCCCCCCCEEEEEEEEECCCCEEEEEECCEEEEECHHHHHHHHHHHHHHHH
MDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1495392 [H]