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The map label for this gene is bcsZ [H]

Identifier: 170683128

GI number: 170683128

Start: 3913693

End: 3914805

Strand: Reverse

Name: bcsZ [H]

Synonym: EcSMS35_3840

Alternate gene names: 170683128

Gene position: 3914805-3913693 (Counterclockwise)

Preceding gene: 170681414

Following gene: 170680722

Centisome position: 77.24

GC content: 54.18

Gene sequence:

>1113_bases
TTGAAGATGAATGTGTTGCGTAGTGGACTCGTGACGATGCTGCTGCTGGCTGCCTTTAGTGTTCAGGCAGCCTGCAACTG
GCCTGCCTGGGAGCAATTCAAAAAGGATTACATCAGTCAGGAAGGGCGCGTCATTGACCCAAGTGACGCGCGTAAAATCA
CCACTTCCGAAGGGCAAAGTTACGGCATGTTCTTTGCCCTGGCGGCTAACGACCGTGCAGCTTTCGATAATATTCTCGAC
TGGACGCAGAACAATCTCGCTCAGGGTTCTTTAAAAGAACGTTTGCCCGCATGGCTGTGGGGCAAGAAAGAGAACAGTAA
GTGGGAAGTGCTGGACAGCAATTCGGCCTCCGATGGTGATGTCTGGATGGCCTGGTCGTTGCTGGAGGCGGGGCGTTTGT
GGAAAGAGCAGCGTTATACCGACATCGGCAGCGCGTTGTTAAAACGTATTGCGCGGGAGGAAGTGGTGACGGTGCCTGGG
CTGGGTTCCATGTTGTTGCCGGGCAAAGTGGGATTTGCAGAGGATAACAGCTGGCGTTTTAACCCCAGCTACCTGCCGCC
GACGCTGGCGCAGTATTTCACCCGCTTTGGCGCGCCGTGGACTACGCTGCGCGAAACCAATCAACGTTTATTGCTGGAAA
CCGCCCCGAAAGGCTTTTCGCCAGACTGGGTGCGCTATGAGAAAGACAAAGGCTGGCAGCTAAAAGCCGAAAAAACATTG
ATCAGCAGCTACGACGCGATCCGCGTTTACATGTGGGTAGGCATGATGCCTGACAGCGATCCGCAAAAAGCGCGGATGCT
CAACCGGTTTAAACCGATGGCGACATTCACCGAGAAAAACGGTTATCCGCCGGAAAAAGTGGATGTGGCTACGGGGAAAG
CACAGGGTAAAGGACCGGTCGGTTTTTCTGCCGCCATGCTGCCCTTTTTACAAAACCGCGATGCGCAGGCCGTTCAGCGC
CAGCGCGTGGCCGATAACTTTCCCGGCAGCGATGCCTATTACAACTATGTGCTGACCCTGTTTGGACAAGGCTGGGATCA
ACACCGTTTCCGCTTCTCGACAAAAGGTGAGTTATTACCTGACTGGGGCCAGGAATGCGCAAATTCACACTAA

Upstream 100 bases:

>100_bases
GGCGGTGCTGGCGGCAATCAGCGTCGTGCTGCTGGCATGGGTACTGTGGCGTTTGCTGCGTATTATCAGCCGTCGTCGTC
TTAACCCGGATAACGAGTAA

Downstream 100 bases:

>100_bases
ACATATTCACGCTTTCCCTCGGTCTGGCCGTCATGCCGATGGTCGAGGCAGCACCAACCGCTCAGCAACAGTTGCTGGAG
CAAGTTCGGTTAGGCGAAGC

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKMNVLRSGLVTMLLLAAFSVQAACNWPAWEQFKKDYISQEGRVIDPSDARKITTSEGQSYGMFFALAANDRAAFDNILD
WTQNNLAQGSLKERLPAWLWGKKENSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPG
LGSMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFSPDWVRYEKDKGWQLKAEKTL
ISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTEKNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQR
QRVADNFPGSDAYYNYVLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH

Sequences:

>Translated_370_residues
MKMNVLRSGLVTMLLLAAFSVQAACNWPAWEQFKKDYISQEGRVIDPSDARKITTSEGQSYGMFFALAANDRAAFDNILD
WTQNNLAQGSLKERLPAWLWGKKENSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPG
LGSMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFSPDWVRYEKDKGWQLKAEKTL
ISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTEKNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQR
QRVADNFPGSDAYYNYVLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH
>Mature_370_residues
MKMNVLRSGLVTMLLLAAFSVQAACNWPAWEQFKKDYISQEGRVIDPSDARKITTSEGQSYGMFFALAANDRAAFDNILD
WTQNNLAQGSLKERLPAWLWGKKENSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPG
LGSMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFSPDWVRYEKDKGWQLKAEKTL
ISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTEKNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQR
QRVADNFPGSDAYYNYVLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=368, Percent_Identity=99.1847826086957, Blast_Score=754, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 42033; Mature: 42033

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS00812 GLYCOSYL_HYDROL_F8

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMNVLRSGLVTMLLLAAFSVQAACNWPAWEQFKKDYISQEGRVIDPSDARKITTSEGQS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEECCCCCCEEECCCCCC
YGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKENSKWEVLDSNSASDGD
CCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHCCHHCCCCCCCCCEEEEECCCCCCCC
VWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLGSMLLPGKVGFAEDNSWRF
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCEEE
NPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFSPDWVRYEKDKGWQLKAEKTL
CCCCCCHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCEEEHHHHH
ISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTEKNGYPPEKVDVATGKAQGKGPV
HHHHHHEEEEEEEECCCCCCHHHHHHHHHHCCHHHECCCCCCCCCCEEECCCCCCCCCCC
GFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNYVLTLFGQGWDQHRFRFSTKGELLP
CHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCC
DWGQECANSH
HHHHHHCCCC
>Mature Secondary Structure
MKMNVLRSGLVTMLLLAAFSVQAACNWPAWEQFKKDYISQEGRVIDPSDARKITTSEGQS
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEECCCCCCEEECCCCCC
YGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKENSKWEVLDSNSASDGD
CCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHCCHHCCCCCCCCCEEEEECCCCCCCC
VWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLGSMLLPGKVGFAEDNSWRF
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCEEE
NPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFSPDWVRYEKDKGWQLKAEKTL
CCCCCCHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCEEEHHHHH
ISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTEKNGYPPEKVDVATGKAQGKGPV
HHHHHHEEEEEEEECCCCCCHHHHHHHHHHCCHHHECCCCCCCCCCEEECCCCCCCCCCC
GFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNYVLTLFGQGWDQHRFRFSTKGELLP
CHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCC
DWGQECANSH
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]