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The map label for this gene is glyS [H]

Identifier: 170682940

GI number: 170682940

Start: 3948477

End: 3950546

Strand: Reverse

Name: glyS [H]

Synonym: EcSMS35_3881

Alternate gene names: 170682940

Gene position: 3950546-3948477 (Counterclockwise)

Preceding gene: 170683810

Following gene: 170683209

Centisome position: 77.94

GC content: 55.07

Gene sequence:

>2070_bases
ATGTCTGAGAAAACTTTTCTGGTGGAAATCGGCACTGAAGAGCTGCCACCAAAAGCACTGCGCAGCCTGGCTGAGTCCTT
TGCTGCGAACTTTACTGCGGAGCTGGATAACGCTGGCCTCGCACACGGCACCGTTCAATGGTTTGCTGCTCCGCGTCGTC
TGGCGCTGAAAGTGGCTAACCTGGCGGAAGCGCAACCGGATCGTGAAATCGAAAAACGCGGCCCGGCAATTGCCCAGGCG
TTCGACGCTGAAGGCAAACCGAGCAAAGCGGCAGAAGGTTGGGCGCGTGGTTGCGGTATTACCGTTGACCAGGCTGAGCG
TCTGACTACCGATAAAGGCGAATGGCTGCTGTATCGCGCCCATGTGAAGGGCGAAAGCACCGAAGCACTGCTGCCGAATA
TGGTTGCGACTTCGCTGGCGAAACTGCCGATCCCGAAACTGATGCGTTGGGGCGCAAGCGACGTGCACTTCGTGCGTCCG
GTTCACACCGTGACCCTGCTGCTGGGCGACAAAGTTATTCCGGCTACCATTCTGGGTATTCAGTCCGATCGCGTGATTCG
CGGTCACCGCTTTATGGGCGAGTCGGAATTCACTATCGACAATGCCGATCAGTATCCGGAAATTCTGCGTGAGCGCGGGA
AAGTCATCGCCGATTACGAAGAACGTAAGGCGAAGATTAAAGCCGATGCCGAAGAAGCGGCGCGTAAGATTGGCGGTAAC
GCTGACTTAAGCGAAAGCCTGTTGGAAGAAGTCGCTTCGCTGGTGGAATGGCCGGTTGTGCTGACCGCAAAATTCGAAGA
GAAATTCCTCGCGGTGCCGTCTGAAGCGCTGGTTTACACCATGAAAGGTGACCAGAAATACTTCCCGGTGTATGCGAACG
ACGGCAAACTGCTGCCGAACTTTATCTTCGTTGCCAATATCGAATCGAAAGATCCGCAGCAAATTATCTCTGGTAACGAG
AAAGTCGTTCGTCCACGTCTGGCGGATGCCGAGTTCTTCTTCAACACCGACCGTAAAAAACGTCTGGAAGATAACCTGCC
GCGCCTGCAAACCGTGTTGTTCCAGCAACAGCTGGGTACACTGCGCGACAAAACTGACCGCATCCAGGCGCTGGCTGGCT
GGATTGCTGAACAGATTGGCGCTGACGTTAACCACGCAACCCGTGCGGGCCTGCTGTCCAAGTGCGACCTGATGACCAAC
ATGGTCTTCGAGTTCACCGACACCCAGGGCGTTATGGGGATGCACTACGCGCGTCACGATGGCGAAGCGGAAGATGTTGC
CGTGGCGCTGAACGAGCAGTATCAGCCGCGCTTTGCCGGTGATGACCTGCCGTCTAACCCGGTAGCCTGTGCGCTGGCGA
TTGCTGACAAGATGGATACTCTGGCGGGTATCTTCGGTATCGGCCAGCATCCGAAAGGCGACAAAGACCCGTTTGCGTTG
CGTCGTGCCGCACTTGGCGTGCTGCGTATTATCGTTGAGAAGAACCTCAATCTTGACCTGCAAACCCTGACCGAAGAAGC
AGTGCGTCTGTATGGCGATAAGCTGACTAATGCCAACGTGGTTGATGATGTTATCGACTTTATGCTCGGTCGCTTCCGCG
CCTGGTATCAGGACGAAGGTTACACCGTTGACACCATCCAGGCGGTACTGGCGCGTCGTCCGACTCGTCCGGCTGATTTC
GATGCCCGAATGAAAGCGGTATCGCACTTCCGTACCCTGGATGCAGCTGCAGCACTGGCGGCGGCGAACAAGCGTGTATC
TAACATTCTGGCGAAATCTGACGAAGTGCTGAGCGACCGCGTGAATGCCTCTACTCTGAAAGAGCCGGAAGAAATTAAAC
TGGCGATGCAGGTTGTGGTGCTACGTGACAAGCTAGAGCCGTACTTTGCTGAAGGTCGTTACCAGGATGCGCTGGTCGAA
CTGGCTGAGCTGCGTGAACCGGTTGATGCCTTCTTCGATAAAGTGATGGTCATGGTTGATGACAAAGAATTGCGAATCAA
CCGTCTTACCATGCTGGAGAAACTGCGCGAATTATTCCTGCGAGTTGCGGATATTTCGCTGTTGCAGTAA

Upstream 100 bases:

>100_bases
GCGCATTCGCACCCTGACCAAAGCAGTGGCAGAAGCATACTACGCTTCCCGTGAAGCCCTCGGCTTCCCGATGTGCAACA
AAGATAAGTAAGAGGCGGCT

Downstream 100 bases:

>100_bases
TAACGTCGTTATTAAAAAGCCTGCCATCTGGCAGGCTTTTTTATCGCTAAATAATAGAGCAACCTTTAATAATCTTCTGC
TGAATAAAGATTGTCTCATA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGTVQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQA
FDAEGKPSKAAEGWARGCGITVDQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGESEFTIDNADQYPEILRERGKVIADYEERKAKIKADAEEAARKIGGN
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEALVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNE
KVVRPRLADAEFFFNTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFAL
RRAALGVLRIIVEKNLNLDLQTLTEEAVRLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADF
DARMKAVSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDKLEPYFAEGRYQDALVE
LAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRVADISLLQ

Sequences:

>Translated_689_residues
MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGTVQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQA
FDAEGKPSKAAEGWARGCGITVDQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGESEFTIDNADQYPEILRERGKVIADYEERKAKIKADAEEAARKIGGN
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEALVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNE
KVVRPRLADAEFFFNTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFAL
RRAALGVLRIIVEKNLNLDLQTLTEEAVRLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADF
DARMKAVSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDKLEPYFAEGRYQDALVE
LAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRVADISLLQ
>Mature_688_residues
SEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGTVQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAF
DAEGKPSKAAEGWARGCGITVDQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRPV
HTVTLLLGDKVIPATILGIQSDRVIRGHRFMGESEFTIDNADQYPEILRERGKVIADYEERKAKIKADAEEAARKIGGNA
DLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEALVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEK
VVRPRLADAEFFFNTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLLSKCDLMTNM
VFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFALR
RAALGVLRIIVEKNLNLDLQTLTEEAVRLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFD
ARMKAVSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDKLEPYFAEGRYQDALVEL
AELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRVADISLLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=99.5645863570392, Blast_Score=1398, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 76790; Mature: 76658

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGTVQWFAAPRRLALKVAN
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
LAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITVDQAERLTTDKGEWLLYRA
HHHCCCCCHHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEE
HVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRPVHTVTLLLGDKVIPATILGI
EECCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEECHHHHHHHHHCCCCHHHHHEEC
QSDRVIRGHRFMGESEFTIDNADQYPEILRERGKVIADYEERKAKIKADAEEAARKIGGN
CCCCEEECHHCCCCCCCEECCCHHHHHHHHHCCCEEECHHHHHHHHCCCHHHHHHHCCCC
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEALVYTMKGDQKYFPVYANDGKLLPN
CCHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCCCCEEEEEECCCEEEEEEECCCCCCCC
FIFVANIESKDPQQIISGNEKVVRPRLADAEFFFNTDRKKRLEDNLPRLQTVLFQQQLGT
EEEEEECCCCCHHHHHCCCCCEECCCCCCCHHEECCCHHHHHHHCHHHHHHHHHHHHHHH
LRDKTDRIQALAGWIAEQIGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHCCC
GEAEDVAVALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFAL
CCHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGVLRIIVEKNLNLDLQTLTEEAVRLYGDKLTNANVVDDVIDFMLGRFRAWYQDEG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
YTVDTIQAVLARRPTRPADFDARMKAVSHFRTLDAAAALAAANKRVSNILAKSDEVLSDR
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHC
VNASTLKEPEEIKLAMQVVVLRDKLEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVD
CCHHHCCCHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEEC
DKELRINRLTMLEKLRELFLRVADISLLQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGTVQWFAAPRRLALKVAN
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
LAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITVDQAERLTTDKGEWLLYRA
HHHCCCCCHHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEE
HVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRPVHTVTLLLGDKVIPATILGI
EECCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEECHHHHHHHHHCCCCHHHHHEEC
QSDRVIRGHRFMGESEFTIDNADQYPEILRERGKVIADYEERKAKIKADAEEAARKIGGN
CCCCEEECHHCCCCCCCEECCCHHHHHHHHHCCCEEECHHHHHHHHCCCHHHHHHHCCCC
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEALVYTMKGDQKYFPVYANDGKLLPN
CCHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCCCCEEEEEECCCEEEEEEECCCCCCCC
FIFVANIESKDPQQIISGNEKVVRPRLADAEFFFNTDRKKRLEDNLPRLQTVLFQQQLGT
EEEEEECCCCCHHHHHCCCCCEECCCCCCCHHEECCCHHHHHHHCHHHHHHHHHHHHHHH
LRDKTDRIQALAGWIAEQIGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHCCC
GEAEDVAVALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFAL
CCHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGVLRIIVEKNLNLDLQTLTEEAVRLYGDKLTNANVVDDVIDFMLGRFRAWYQDEG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
YTVDTIQAVLARRPTRPADFDARMKAVSHFRTLDAAAALAAANKRVSNILAKSDEVLSDR
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHC
VNASTLKEPEEIKLAMQVVVLRDKLEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVD
CCHHHCCCHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEEC
DKELRINRLTMLEKLRELFLRVADISLLQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA