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The map label for this gene is mdtK [H]

Identifier: 170682804

GI number: 170682804

Start: 1518029

End: 1519402

Strand: Reverse

Name: mdtK [H]

Synonym: EcSMS35_1534

Alternate gene names: 170682804

Gene position: 1519402-1518029 (Counterclockwise)

Preceding gene: 170682135

Following gene: 170680041

Centisome position: 29.98

GC content: 53.28

Gene sequence:

>1374_bases
GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGC
GATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGAACTTCTATCT
GGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGCTCCGGTCGA
CGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCATTATGCTGGTGCTGTG
GAATGCAGGCTACATTATCCGCTCCATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGT
TGCTATGGGGCGCGCCGGGCTATCTTTTCTTCCAGGTTGCCCGTAACCAGTGTGAAGGTCTGGCAAAAACCAAGCCGGGT
ATGGTGATGGGCTTTATCGGCCTGCTGGTGAACATTCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGA
GCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCATGTTCCTTGCCATGGTTTCTTACATTAAAC
GCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAA
CTCGGTTTGCCAATTGCGCTGGCACTGTTCTTTGAAGTAACGCTGTTTGCCGTCGTGGCTCTGTTAGTGTCTCCGCTCGG
TATTGTTGATGTCGCAGGACATCAGATTGCCCTGAACTTTAGTTCACTGATGTTCGTGCTTCCCATGTCGCTGGCGGCAG
CGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAAGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATG
GTGGGTGTCTGTATGGCAACCCTGACGGCCATATTCACGGTTTCACTGCGAGAGCAAATCGCCCTGTTGTACAACGACAA
TCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGCTGCTGGCGGCGGTATATCAGATTTCTGACTCTATCCAGGTGATTG
GCAGTGGGATTTTGCGTGGTTATAAAGATACACGTTCCATTTTCTATATCACCTTTACGGCTTACTGGGTGCTGGGCTTG
CCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTAT
TGGCCTGACTTCGGCAGCCATTATGATGATGCTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAAC
GAGCAGCTCGCTAG

Upstream 100 bases:

>100_bases
TCCTCGCTACAATAGACTGAATTTCCCCTGCTTCTTCTTTTTGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAAC
TACAAATAAAAGGTGTTCAC

Downstream 100 bases:

>100_bases
TAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTGTTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCT
GGCTGTAGTTTCAGTGCGTT

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPDPAVMKRLIQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLM
VGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITFTAYWVLGL
PSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAIILQRAAR

Sequences:

>Translated_457_residues
MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPDPAVMKRLIQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLM
VGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITFTAYWVLGL
PSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAIILQRAAR
>Mature_457_residues
MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPDPAVMKRLIQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLM
VGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITFTAYWVLGL
PSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAIILQRAAR

Specific function: Multidrug efflux pump that functions probably as a Na(+)/drug antiporter [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MdtK subfamily [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=442, Percent_Identity=21.4932126696833, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI153792564, Length=424, Percent_Identity=20.0471698113208, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI38261960, Length=458, Percent_Identity=19.8689956331878, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI48994890, Length=457, Percent_Identity=99.781181619256, Blast_Score=911, Evalue=0.0,
Organism=Escherichia coli, GI226510954, Length=459, Percent_Identity=23.7472766884532, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321821, Length=388, Percent_Identity=20.6185567010309, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6320545, Length=439, Percent_Identity=21.4123006833713, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR022913 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49432; Mature: 49432

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRSMENID
HCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCC
PALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPGMVMGFIGLLVNIPVNYIFIY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHCCEEEEEEE
GHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPDPAVMKRLIQ
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVG
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHC
YRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHH
AAVYQISDSIQVIGSGILRGYKDTRSIFYITFTAYWVLGLPSGYILALTDLVVEPMGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCHH
FWIGFIIGLTSAAIMMMLRMRFLQRLPSAIILQRAAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRSMENID
HCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCC
PALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPGMVMGFIGLLVNIPVNYIFIY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHCCEEEEEEE
GHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPDPAVMKRLIQ
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVG
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHC
YRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHH
AAVYQISDSIQVIGSGILRGYKDTRSIFYITFTAYWVLGLPSGYILALTDLVVEPMGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCHH
FWIGFIIGLTSAAIMMMLRMRFLQRLPSAIILQRAAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA