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The map label for this gene is rfaL [H]

Identifier: 170682768

GI number: 170682768

Start: 4037015

End: 4038268

Strand: Reverse

Name: rfaL [H]

Synonym: EcSMS35_3959

Alternate gene names: 170682768

Gene position: 4038268-4037015 (Counterclockwise)

Preceding gene: 170681613

Following gene: 170682822

Centisome position: 79.68

GC content: 33.81

Gene sequence:

>1254_bases
ATGTCGTTTTGTTGGAAAGAAATTAACTCTGGTGTCAAGTCTTTAATTCTCATATTATGTATGTTGTCTTTAATGACTTT
GTCTTTGTGGGATGATGTTGCAACAAAGTTTCTTCATGCAGCTGGAATTATATCTACATTATATTTTCTAGCGACAGCAA
AAAAAACAATAACTAATAATCCTACTTTGTTAATTTTTATCTCATTATGTCTTTTGGGTATCGTTAATATCATCTGGTAT
TCACATTATAAAGTTTCAGGCTCGGTTTATACCAATGCATATCGTGGCCCAATGGAAACTGGAAAAATTGCCTTGTGTAG
TGCTTTTATTTTCTTAGTTCTTTTTGCTAAAGATGAGATGAGAACAAAAATAAAATTTGGGAAACTAATTCTGTTCGCAT
CCCTGGCAACGCAGTTACTTTTTTTTGCGCATGCCATGTGGCAACATTTCTATTTAAACGTAGACCGTGTTGCATTATCA
GCTTCCCACGCTACAACAGCAGGCTACATCATCCTTTTCCCTTCTTTACTGGCATCAATTCTCATTTTAAAATGCGACTT
TAGACATAAAACAACATTATATACAATTAACTTCATGCTTAGCTTATGTGCTGTCATAGTAACTGAAACGCGTGCAGCCA
TATTAGTGTTTCCATTCTTTGCGTTAATATTAATCGTAATGGATAGTTATATTAATAAGCGAATTAATTATAAGTTATAT
TGTTTTATTGCGATTGCATTATTAGCAGGTGTGTTTTCTTTTAAAGATACATTGCTTATGAGAATGAATGACTTAAATAA
CGATTTAGTTAATTATTCGCATGATAACACCAGAACTTCAGTCGGTGCCCGTCTGGCAATGTATGAAGTTGGTTTAAAAA
CATATTCTCCAATAGGACAATCACTGGAAAAACGCGCGGAAAAAATACATGAACTAGAAGAGAAAGAGCCTAGATTAAGT
GGCGCTTTACCCTTTGTAGATTCTCATTTGCATAACGATCTCATAGATACGTTATCAACGCGTGGTATTCCTGGAGTAGT
ATTAACAATTTTAGCCTTTTCAGCAATATTCATATATGCCTTAAGAACTGCTAAAGAACCTTATATTTTAATCTTGCTTT
TTTCACTACTGGTAGTAGGCCTAAGTGATGTAATACTCTTTTCTAAACCGGTTCCGACTGCTGTGTTTGTCACCATAATA
TTGCTTTGTGCTTATTTTAAAGCACAATCGGACCAATGTTTATTAGATAAGTAA

Upstream 100 bases:

>100_bases
CTATATTCTCTGGAGGATGGTAAGACTGGTAAATAGAAAAAAGAATAAACGTTAGGTCTTGCATTAAATAACCCATCCTC
GTAGCATAGGTTGAAATTAT

Downstream 100 bases:

>100_bases
TGAATATGTGAAATAAAATCAGCAAGGAAGCTGATTTTATTTCAAAATTATCTATTAAAGAGTTGTAATTTAAATCATGG
CAGCGTTTTCTTCAATGAAC

Product: O-antigen polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSFCWKEINSGVKSLILILCMLSLMTLSLWDDVATKFLHAAGIISTLYFLATAKKTITNNPTLLIFISLCLLGIVNIIWY
SHYKVSGSVYTNAYRGPMETGKIALCSAFIFLVLFAKDEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
ASHATTAGYIILFPSLLASILILKCDFRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALILIVMDSYINKRINYKLY
CFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLS
GALPFVDSHLHNDLIDTLSTRGIPGVVLTILAFSAIFIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTII
LLCAYFKAQSDQCLLDK

Sequences:

>Translated_417_residues
MSFCWKEINSGVKSLILILCMLSLMTLSLWDDVATKFLHAAGIISTLYFLATAKKTITNNPTLLIFISLCLLGIVNIIWY
SHYKVSGSVYTNAYRGPMETGKIALCSAFIFLVLFAKDEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
ASHATTAGYIILFPSLLASILILKCDFRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALILIVMDSYINKRINYKLY
CFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLS
GALPFVDSHLHNDLIDTLSTRGIPGVVLTILAFSAIFIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTII
LLCAYFKAQSDQCLLDK
>Mature_416_residues
SFCWKEINSGVKSLILILCMLSLMTLSLWDDVATKFLHAAGIISTLYFLATAKKTITNNPTLLIFISLCLLGIVNIIWYS
HYKVSGSVYTNAYRGPMETGKIALCSAFIFLVLFAKDEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALSA
SHATTAGYIILFPSLLASILILKCDFRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALILIVMDSYINKRINYKLYC
FIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSG
ALPFVDSHLHNDLIDTLSTRGIPGVVLTILAFSAIFIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIIL
LCAYFKAQSDQCLLDK

Specific function: Adds the O-antigen on the glucose group of LPS [H]

COG id: COG3307

COG function: function code M; Lipid A core - O-antigen ligase and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790052, Length=382, Percent_Identity=30.3664921465969, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007016 [H]

Pfam domain/function: PF04932 Wzy_C [H]

EC number: NA

Molecular weight: Translated: 46794; Mature: 46663

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFCWKEINSGVKSLILILCMLSLMTLSLWDDVATKFLHAAGIISTLYFLATAKKTITNN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGKIALCSAFIFLVLFAKDEM
CHHHHHHHHHHHHHHHHHHHHCEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHHHHHHHHHHHH
LILKCDFRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALILIVMDSYINKRINYKLY
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
CFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDLIDTLSTRGIPGVVLTILAFSAIFIYA
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIILLCAYFKAQSDQCLLDK
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SFCWKEINSGVKSLILILCMLSLMTLSLWDDVATKFLHAAGIISTLYFLATAKKTITNN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGKIALCSAFIFLVLFAKDEM
CHHHHHHHHHHHHHHHHHHHHCEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
RTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHHHHHHHHHHHH
LILKCDFRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALILIVMDSYINKRINYKLY
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
CFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDLIDTLSTRGIPGVVLTILAFSAIFIYA
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIILLCAYFKAQSDQCLLDK
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1624462; 8041620; 9278503 [H]