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The map label for this gene is gabT1 [H]

Identifier: 170682308

GI number: 170682308

Start: 1839045

End: 1840310

Strand: Reverse

Name: gabT1 [H]

Synonym: EcSMS35_1820

Alternate gene names: 170682308

Gene position: 1840310-1839045 (Counterclockwise)

Preceding gene: 170679683

Following gene: 170680978

Centisome position: 36.31

GC content: 57.58

Gene sequence:

>1266_bases
ATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCTACCCCTCGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCA
GTCGGCAGAAAACGCCATGCTGAAGGACGTAGAGGGCAACGAATACATCGATTTCGCCGCAGGCATTGCGGTGCTGAATA
CCGGGCATCGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAATTGCAACAGTTTACCCACACGGCGTATCAGATTGTG
CCGTACGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCCCTTGCCCCGGTGAGCGGGCAGGCTAAAACTGCGTTCTT
CACCACCGGTGCGGAAGCGGTGGAGAACGCGGTGAAAATCGCCCGCGCCCATACCGGACGCCCTGGCGTGATTGCGTTTA
GCGGCGGCTTCCACGGTCGTACATATATGACCATGGCGCTGACCGGAAAGGTCGCGCCGTACAAAATCGGCTTCGGCCCG
TTCCCCGGTTCGGTATATCACGTACCTTATCCGTCAGATTTACATGGCGTTTCAACGCAGGACTCTCTCGACGCCATCGA
ACGCTTGTTTAAATCTGACATTGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAAGGCGGTTTCA
ACGTTGCACCAAAAGAGCTGGTTGCCGCCATTCGCCGCCTGTGCGACGAGCACGGCATTGTGATGATTGCCGATGAAGTG
CAAAGCGGCTTTGCGCGTACCGGTAAACTGTTTGCCATGGATCACTACGCCGATAAGCCGGACTTAATGACGATGGCGAA
AAGCCTCGCGGGCGGCATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCGCCCGCGCCGGGCGGGTTGG
GTGGTACTTACGCCGGGAACCCGCTGGCGGTGGCTGCCGCGCACGCTGTGCTCAACATTATCGACAAAGAATCACTCTGT
GAACGCGCGAATCAACTGGGCCAGCGCCTGACAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATCGCGGCGGT
ACGCGGTCTGGGGTCTATGATTGCGGCAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCACAGAAAA
TCCAGCAACGCGCGCTGGCGCAGGGGCTGCTTCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGTTTCCTGTATCCG
CTGACCATCCCGGATGCGCAATTCGATGCGGCAATGAAAATTTTGCAGGATGCGCTGAAAGATTAA

Upstream 100 bases:

>100_bases
CGCGTGCCATTTACCGCGATGGGCGCGGCTTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACAGTGA
AGAGTCAAAAGGTGTGAAAC

Downstream 100 bases:

>100_bases
TACGCAGAAGCAGGGTCAACGCAATAATGTCTGATACGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATG
CCGGATAAGGCGTTCACGCC

Product: 4-aminobutyrate transaminase

Products: NA

Alternate protein names: GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSNNEFHQRRLSATPRGVGVMCNFFAQSAENAMLKDVEGNEYIDFAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIV
PYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
FPGSVYHVPYPSDLHGVSTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEV
QSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLC
ERANQLGQRLTNTLIDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYP
LTIPDAQFDAAMKILQDALKD

Sequences:

>Translated_421_residues
MSNNEFHQRRLSATPRGVGVMCNFFAQSAENAMLKDVEGNEYIDFAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIV
PYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
FPGSVYHVPYPSDLHGVSTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEV
QSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLC
ERANQLGQRLTNTLIDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYP
LTIPDAQFDAAMKILQDALKD
>Mature_420_residues
SNNEFHQRRLSATPRGVGVMCNFFAQSAENAMLKDVEGNEYIDFAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVP
YESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGPF
PGSVYHVPYPSDLHGVSTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEVQ
SGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCE
RANQLGQRLTNTLIDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPL
TIPDAQFDAAMKILQDALKD

Specific function: Involved in the breakdown of putrescine via transamination of gamma-aminobutyrate [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=400, Percent_Identity=32.5, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI284507298, Length=322, Percent_Identity=32.6086956521739, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI13994255, Length=417, Percent_Identity=30.2158273381295, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI37574042, Length=448, Percent_Identity=29.0178571428571, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI188536080, Length=445, Percent_Identity=28.314606741573, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI38679950, Length=445, Percent_Identity=28.314606741573, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI38679946, Length=445, Percent_Identity=28.314606741573, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI226442709, Length=389, Percent_Identity=30.0771208226221, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI226442705, Length=448, Percent_Identity=28.5714285714286, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI24119277, Length=392, Percent_Identity=27.5510204081633, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1787560, Length=421, Percent_Identity=98.812351543943, Blast_Score=854, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=421, Percent_Identity=54.3942992874109, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI1789759, Length=403, Percent_Identity=33.7468982630273, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1788044, Length=395, Percent_Identity=34.1772151898734, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI145693181, Length=395, Percent_Identity=35.1898734177215, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1786349, Length=348, Percent_Identity=31.6091954022989, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1786991, Length=409, Percent_Identity=27.8728606356968, Blast_Score=132, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI25144271, Length=407, Percent_Identity=32.9238329238329, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI32564660, Length=422, Percent_Identity=31.7535545023697, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI71992977, Length=410, Percent_Identity=30.7317073170732, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25144274, Length=295, Percent_Identity=32.5423728813559, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17541228, Length=427, Percent_Identity=25.7611241217799, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71981843, Length=155, Percent_Identity=33.5483870967742, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=406, Percent_Identity=32.512315270936, Blast_Score=189, Evalue=8e-49,
Organism=Saccharomyces cerevisiae, GI6324432, Length=406, Percent_Identity=31.7733990147783, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6324386, Length=314, Percent_Identity=25.1592356687898, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6321456, Length=385, Percent_Identity=26.2337662337662, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21357415, Length=404, Percent_Identity=31.9306930693069, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI161085790, Length=419, Percent_Identity=29.8329355608592, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI28574759, Length=409, Percent_Identity=30.5623471882641, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21356575, Length=429, Percent_Identity=28.4382284382284, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI281366494, Length=378, Percent_Identity=26.7195767195767, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24667139, Length=378, Percent_Identity=26.7195767195767, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24667143, Length=378, Percent_Identity=26.7195767195767, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004632
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.19 [H]

Molecular weight: Translated: 44732; Mature: 44600

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNEFHQRRLSATPRGVGVMCNFFAQSAENAMLKDVEGNEYIDFAAGIAVLNTGHRHPD
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCEEHHHHHHHHCCCCCCCHH
LVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKI
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHH
ARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGPFPGSVYHVPYPSDLHGVSTQ
HHHCCCCCCEEEECCCCCCCEEEEEEEECCCCCEEECCCCCCCCEEECCCCCCCCCCCCH
DSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEV
HHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHH
QSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGN
HCCHHHCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCEECCCCEECCCCCCCCCCCCCCC
PLAVAAAHAVLNIIDKESLCERANQLGQRLTNTLIDAKESVPAIAAVRGLGSMIAAEFND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
PQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQFDAAMKILQDALK
CCCCCCHHHHHHHHHHHHHHCCHHEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
D
C
>Mature Secondary Structure 
SNNEFHQRRLSATPRGVGVMCNFFAQSAENAMLKDVEGNEYIDFAAGIAVLNTGHRHPD
CCCHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCEEHHHHHHHHCCCCCCCHH
LVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKI
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHH
ARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGPFPGSVYHVPYPSDLHGVSTQ
HHHCCCCCCEEEECCCCCCCEEEEEEEECCCCCEEECCCCCCCCEEECCCCCCCCCCCCH
DSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEV
HHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHH
QSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGN
HCCHHHCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCEECCCCEECCCCCCCCCCCCCCC
PLAVAAAHAVLNIIDKESLCERANQLGQRLTNTLIDAKESVPAIAAVRGLGSMIAAEFND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
PQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQFDAAMKILQDALK
CCCCCCHHHHHHHHHHHHHHCCHHEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]