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The map label for this gene is yneJ [H]

Identifier: 170682090

GI number: 170682090

Start: 1633448

End: 1634329

Strand: Reverse

Name: yneJ [H]

Synonym: EcSMS35_1646

Alternate gene names: 170682090

Gene position: 1634329-1633448 (Counterclockwise)

Preceding gene: 170681176

Following gene: 170680980

Centisome position: 32.25

GC content: 54.2

Gene sequence:

>882_bases
ATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATTACCCAGGCTGCAGCAAAAGTGCATCG
CGTGCCGTCGAATCTCACTACCCGTCTACGCCAGCTGGAAACAGAACTGGGGATTGATCTGTTTATTCGCGAGAATCAGC
GTTTACGTCTCTCACCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGC
GTTGTCGCTGGCGATGAGCCGCAAGGTTTATTTTCTCTTGGTTCGCTGGAAAGCACCGCTGCGGTGCGCATTCCGGTCAC
GCTGGCGGAATTTAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGAAGGGG
TGCTGGAGGGAAAACTGAATGCAGCGTTTATTGATGGGCCCATTAACCATACTGCCATTGACGGGATACCGGTGTACCGC
GAGGAGCTGATGATCGTCACGCCACAAGGACATGCGCCAGTAACCCGCGCCAGTCAGGTAAATGGCAGTAACATTTATGC
CTTCCGCGCCAATTGTTCGTATCGTCGCCACTTCGAGAGCTGGTTTCATGCTGATGGTGCCGCTCCGGGAACTATTCACG
AGATGGAGTCGTATCACGGGATGTTGGCCTGTGTGGTCGCAGGAGCAGGTATTGCGCTTATTCCGCGCTCTATGCTGGAA
AGTATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGCGTTGGTTAACAACCTGGCTGGTCTGGCG
TCGCGGTGCGAAAACGCGTCCGCTCGAGGCATTTATTCAACTGCTGGATGTGCCTGACTCGGCAAAACAGGGTTACCAAT
GA

Upstream 100 bases:

>100_bases
CGGGGTATCTCCTTTATGGGTTATGGTAAAAAGATACGCAGATTTACTCTTGCTTGAAAATGAATAATATTAAGTCACTT
ATTCACGAATTGAGAATGCC

Downstream 100 bases:

>100_bases
GCTATTTTTGATAGTTCTTGCGGCTAATATGCTCTATATAGTGATGTTCCGATGACTTATGACTATATGGAGCAAATATG
GTTACGCCAGTAAGCATCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELGIDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARS
VVAGDEPQGLFSLGSLESTAAVRIPVTLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLDVPDSAKQGYQ

Sequences:

>Translated_293_residues
MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELGIDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARS
VVAGDEPQGLFSLGSLESTAAVRIPVTLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLDVPDSAKQGYQ
>Mature_293_residues
MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELGIDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARS
VVAGDEPQGLFSLGSLESTAAVRIPVTLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLDVPDSAKQGYQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=293, Percent_Identity=97.9522184300341, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1788887, Length=293, Percent_Identity=25.938566552901, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082132, Length=287, Percent_Identity=23.3449477351916, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790262, Length=290, Percent_Identity=23.7931034482759, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1790208, Length=256, Percent_Identity=25, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=285, Percent_Identity=24.2105263157895, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788481, Length=180, Percent_Identity=30, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI145693105, Length=289, Percent_Identity=21.7993079584775, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787879, Length=285, Percent_Identity=24.2105263157895, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788297, Length=261, Percent_Identity=25.2873563218391, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790399, Length=289, Percent_Identity=21.7993079584775, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788706, Length=147, Percent_Identity=29.2517006802721, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32471; Mature: 32471

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELGIDLFIRENQRLRLSPA
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCEEEECCC
GHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAVRIPVTLAEFNRRYPKIQF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEEEEHHHHCCCCCEEEE
SLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYREELMIVTPQGHAPVTRASQV
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCEECCCCEECCCEEEECCCCCCCCCCCCCC
NGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLDVPDSAKQGYQ
HCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCCC
>Mature Secondary Structure
MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELGIDLFIRENQRLRLSPA
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCEEEECCC
GHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAVRIPVTLAEFNRRYPKIQF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEEEEHHHHCCCCCEEEE
SLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYREELMIVTPQGHAPVTRASQV
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCEECCCCEECCCEEEECCCCCCCCCCCCCC
NGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLEAFIQLLDVPDSAKQGYQ
HCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]