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The map label for this gene is yeaD [H]

Identifier: 170681442

GI number: 170681442

Start: 1395579

End: 1396463

Strand: Reverse

Name: yeaD [H]

Synonym: EcSMS35_1411

Alternate gene names: 170681442

Gene position: 1396463-1395579 (Counterclockwise)

Preceding gene: 170679626

Following gene: 170684310

Centisome position: 27.55

GC content: 49.83

Gene sequence:

>885_bases
ATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACTGGA
CCTCATTGTGGTCGATCATCCCCAGGTAAAAGCCTCTTTTGCATTACAGGGCGCACACCTTCTCTCATGGAAACCTGCGG
GTGAAGAAGAAGTTCTGTGGTTGAGCAATAACACACCGTTCAAAAATGGCGTCGCTATTCGCGGTGGCGTACCGGTTTGC
TGGCCGTGGTTTGGTCCGGCTGCACAACAAGGTCTGCCTGCGCACGGTTTTGCCCGCAACCTGCCGTGGACGCTGAAATC
ACATCGTGAAGATGCTGATGGCGTAGCGCTGACTTTTGAATTGACACAAAGCGAAGAGACGAAAAAATTCTGGCTACACG
ACTTTACGCTATTAGCGCATTTCCGCGTGGGTAAAGCCTGTGAAATCGATCTTGAAGCACACGGCGAATTTGAAACCACC
TCTGCCCTGCATACCTACTTTAACGTGGGTGATATCACTAAGGTAAGCGTCAGTGGACTGGGCGATCGCTTCATTGATAA
AGTGAATGACGCGAAAGAAGATGTACTGACCGACGGTATTCAGACCTTCCCTGACCGTACCGATCGCGTGTATCTGAATC
CGCAAGATTGCAGCGTAATTAATGACGAAGCGCTGAATCGTATTATCGCCGTAGGCCACCAGCATCATCTGAACGTTGTA
GGCTGGAACCCAGGACCGGCGCTTTCAGTTAGCATGGGCGATATGCCGGATGATGGCTACAAGACCTTTGTTTGTGTAGA
AACAGCTTACGCCTCAGAAACGCAAAAAGTCACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAAC
GTTAA

Upstream 100 bases:

>100_bases
CTCACATCTCCAAATAAGTTGAGATGACACTGTGATCTAAAAAGAGCGACTTCGGTCGCTCTTTTTTTTACCTGATAAAA
TGAAGTTAAAGGACTGCGTC

Downstream 100 bases:

>100_bases
TTTGCGTTAATGTTGTGTGCCGGGTGCGATGCATCCGGCACGTATCAACATCACACCATATCCAGCGCAGTTTTTCCTTT
TGGTGCCGGATATGCCTTAT

Product: aldose 1-epimerase family protein

Products: NA

Alternate protein names: Putative D-hexose-6-phosphate mutarotase; Unknown protein from 2D-page spots T26/PR37 [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQGAHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVC
WPWFGPAAQQGLPAHGFARNLPWTLKSHREDADGVALTFELTQSEETKKFWLHDFTLLAHFRVGKACEIDLEAHGEFETT
SALHTYFNVGDITKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCSVINDEALNRIIAVGHQHHLNVV
GWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQKVTKEKPAHLAQSIRVAKR

Sequences:

>Translated_294_residues
MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQGAHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVC
WPWFGPAAQQGLPAHGFARNLPWTLKSHREDADGVALTFELTQSEETKKFWLHDFTLLAHFRVGKACEIDLEAHGEFETT
SALHTYFNVGDITKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCSVINDEALNRIIAVGHQHHLNVV
GWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQKVTKEKPAHLAQSIRVAKR
>Mature_294_residues
MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQGAHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVC
WPWFGPAAQQGLPAHGFARNLPWTLKSHREDADGVALTFELTQSEETKKFWLHDFTLLAHFRVGKACEIDLEAHGEFETT
SALHTYFNVGDITKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCSVINDEALNRIIAVGHQHHLNVV
GWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQKVTKEKPAHLAQSIRVAKR

Specific function: Unknown

COG id: COG0676

COG function: function code G; Uncharacterized enzymes related to aldose 1-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glucose-6-phosphate 1-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87081973, Length=294, Percent_Identity=97.6190476190476, Blast_Score=597, Evalue=1e-172,
Organism=Saccharomyces cerevisiae, GI6323746, Length=265, Percent_Identity=30.188679245283, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19921284, Length=247, Percent_Identity=28.7449392712551, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008183
- InterPro:   IPR011013
- InterPro:   IPR014718 [H]

Pfam domain/function: PF01263 Aldose_epim [H]

EC number: =5.1.3.15 [H]

Molecular weight: Translated: 32672; Mature: 32672

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQGAHLLSWKPAGEEEVLW
CCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCEEEEECCCCCCEEEE
LSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLPWTLKSHREDADGVALTFE
EECCCCCCCCEEEECCCCEEECCCCCHHHCCCCCCCHHCCCCCHHHCCCCCCCCEEEEEE
LTQSEETKKFWLHDFTLLAHFRVGKACEIDLEAHGEFETTSALHTYFNVGDITKVSVSGL
ECCCCCCHHHHHHHHHEEHHHCCCCEEEEEEECCCCCCHHHHHHHEECCCCEEEEECCCC
GDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCSVINDEALNRIIAVGHQHHLNVV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEECHHHHHHHHHCCCCCEEEEE
GWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQKVTKEKPAHLAQSIRVAKR
EECCCCEEEEECCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQGAHLLSWKPAGEEEVLW
CCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCEEEEECCCCCCEEEE
LSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLPWTLKSHREDADGVALTFE
EECCCCCCCCEEEECCCCEEECCCCCHHHCCCCCCCHHCCCCCHHHCCCCCCCCEEEEEE
LTQSEETKKFWLHDFTLLAHFRVGKACEIDLEAHGEFETTSALHTYFNVGDITKVSVSGL
ECCCCCCHHHHHHHHHEEHHHCCCCEEEEEEECCCCCCHHHHHHHEECCCCEEEEECCCC
GDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCSVINDEALNRIIAVGHQHHLNVV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEECHHHHHHHHHCCCCCEEEEE
GWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQKVTKEKPAHLAQSIRVAKR
EECCCCEEEEECCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 9298646; 10493123 [H]