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The map label for this gene is dadA [H]

Identifier: 170681298

GI number: 170681298

Start: 1982843

End: 1984147

Strand: Reverse

Name: dadA [H]

Synonym: EcSMS35_1960

Alternate gene names: 170681298

Gene position: 1984147-1982843 (Counterclockwise)

Preceding gene: 170681324

Following gene: 170681793

Centisome position: 39.15

GC content: 56.4

Gene sequence:

>1305_bases
GTGGCTATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGA
GGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATG
CAGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGT
CTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAA
AGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAG
GGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAA
GATGCCGGCGTACCGTACCAGCTGCTGGAATCCAGCCGTCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAA
ACTGACGGGGGGGCTCCAGCTGCCCAATGATGAAACCGGAGACTGCCAGCTGTTTACCCAGAATCTGGCGCGGATGGCGG
AGCAGGCGGGGGTTAAATTCCGCTTTAATACGCCCGTTGACCAACTACTTTGCGACGGCGAGCAAATCTACGGCGTGAAG
TGTGGCGATGAAGTGATTAAGGCCGATGCATATGTGATGGCATTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGT
TGATATTCCGGTGTATCCGCTGAAAGGCTACTCGCTGACTATTCCGATTGCACAGGAAGATGGTGCGCCGGTGTCTACCA
TCCTTGATGAAACCTACAAAATCGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGTGGAATGGCGGAAATTGTTGGC
TTTAATACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGT
CGAGCAGGCGACTTTCTGGACTGGTCTGCGCCCGATGACGCCAGACGGCACGCCGGTTGTCGGGCGTACACGCTTTAAAA
ATCTGTGGCTGAATACCGGTCACGGCACGCTCGGCTGGACGATGGCTTGCGGTTCCGGTCAGTTGTTAAGCGATCTGCTC
TCTGGTCGCACGCCAGCGATCCCGTATGAAGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGCCCGGG
CCATTTACATGGCGCACACAGCTAA

Upstream 100 bases:

>100_bases
CAGCTCACCATAAAAAAGCCGTATGTTGAATAATATTTTCAACTGAGTTATCAAGATGTAATTAGATTATTATTCTTTTA
CTGTATCTACCGTTATCGGA

Downstream 100 bases:

>100_bases
GGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGCCAGGC
CGCGCCGCGCGCGCGCGTCT

Product: D-amino acid dehydrogenase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MAMRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANAGQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVR
LDGTQFQLKWMWQMLRNCDTSHYMENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLE
DAGVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVK
CGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVG
FNTELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDLL
SGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS

Sequences:

>Translated_434_residues
MAMRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANAGQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVR
LDGTQFQLKWMWQMLRNCDTSHYMENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLE
DAGVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVK
CGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVG
FNTELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDLL
SGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS
>Mature_433_residues
AMRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANAGQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRL
DGTQFQLKWMWQMLRNCDTSHYMENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLED
AGVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVKC
GDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGF
NTELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDLLS
GRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=432, Percent_Identity=100, Blast_Score=896, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 47810; Mature: 47679

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANAGQISPGYAAPWAAP
CEEEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCC
GVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYMENKGRMVRLAEYSRDC
CCCHHHHHHHHHCCCCEEEEECCCEEHHHHHHHHHHCCCHHHHHHCCCCEEEEHHHHHHH
LKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDAGVPYQLLESSRLAEVEPA
HHHHHHHCCEEECCCCCCEEEEEECHHHHHCHHCCEEEEECCCCCHHHHCCCCCHHCCHH
LAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVK
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCEEEEEE
CGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYK
CCHHHHHCCEEEEEECCHHHHHHHHHHHCCCEEECCCEEEEEEECCCCCCHHHHHCCCEE
IAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMT
EEEEEECCEEEECCEEEEECCCHHHHCCHHHHHHHHHHHHCCCCCCCCHHHEECCCCCCC
PDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDLLSGRTPAIPYEDLSVARYSRG
CCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHCC
FTPSRPGHLHGAHS
CCCCCCCCCCCCCC
>Mature Secondary Structure 
AMRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANAGQISPGYAAPWAAP
EEEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCC
GVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYMENKGRMVRLAEYSRDC
CCCHHHHHHHHHCCCCEEEEECCCEEHHHHHHHHHHCCCHHHHHHCCCCEEEEHHHHHHH
LKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDAGVPYQLLESSRLAEVEPA
HHHHHHHCCEEECCCCCCEEEEEECHHHHHCHHCCEEEEECCCCCHHHHCCCCCHHCCHH
LAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVK
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCEEEEEE
CGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYK
CCHHHHHCCEEEEEECCHHHHHHHHHHHCCCEEECCCEEEEEEECCCCCCHHHHHCCCEE
IAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMT
EEEEEECCEEEECCEEEEECCCHHHHCCHHHHHHHHHHHHCCCCCCCCHHHEECCCCCCC
PDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDLLSGRTPAIPYEDLSVARYSRG
CCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHCC
FTPSRPGHLHGAHS
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA