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The map label for this gene is puuR

Identifier: 170680966

GI number: 170680966

Start: 1843267

End: 1843824

Strand: Reverse

Name: puuR

Synonym: EcSMS35_1823

Alternate gene names: 170680966

Gene position: 1843824-1843267 (Counterclockwise)

Preceding gene: 170679609

Following gene: 170680173

Centisome position: 36.38

GC content: 50.72

Gene sequence:

>558_bases
ATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAGCAGGGGCTTTCACAACGTCGTGCCGC
CGAACTCTCCGGGCTGACTCACAGTGCTATCAGTACGATAGAACAAGATAAAGTCAGCCCTGCCATCAGTACGCTGCAAA
AGCTGCTGAAGGTGTATGGTCTGTCACTCTCGGAATTCTTTTCCGAGCCGGAAAAACCTGATGAGCCGCAGGTCGTCATT
AATCAGGACGACTTAATTGAGATGGGCAGTCAGGGTGTGTCAATGAAGCTGGTTCATAACGGTAACCCGAATCGCACGCT
GGCGATGATCTTTGAAACGTACCAGCCGGGCACAACCACTGGGGAAAGAATTAAGCATCAGGGTGAGGAAATAGGCACTG
TACTGGAAGGTGAAATTGTTCTGACGATTAATGGTCAGGATTATCATCTCGTCGCGGGGCAAAGTTATGCCATTAATACC
GGCATCCCGCACAGTTTCAGTAATACGTCGGCAGGTATTTGCCGAATTATCAGCGCCCATACGCCCACCACGTTTTAA

Upstream 100 bases:

>100_bases
CGCTTTCGCGTATATTGTTCGAGGGCTTTATCACCGCTTGTCAGCACCATATCGCTGAAAAACAGCGACTCTGACCACTA
CAGTTTAAGGAAATGCAAAT

Downstream 100 bases:

>100_bases
TCTTTTTGTTCTGTAAGCCGGGTAAGCGCAATGCGCCCGGCAATCCATAAAGAATGTCGCCAGAAATAAACGGGCATACG
GCCCGGGGATCTCTGCGCCC

Product: DNA-binding transcriptional repressor PuuR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDKVSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVI
NQDDLIEMGSQGVSMKLVHNGNPNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
GIPHSFSNTSAGICRIISAHTPTTF

Sequences:

>Translated_185_residues
MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDKVSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVI
NQDDLIEMGSQGVSMKLVHNGNPNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
GIPHSFSNTSAGICRIISAHTPTTF
>Mature_184_residues
SDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDKVSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVIN
QDDLIEMGSQGVSMKLVHNGNPNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINTG
IPHSFSNTSAGICRIISAHTPTTF

Specific function: Represses puuD

COG id: COG1396

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787557, Length=185, Percent_Identity=100, Blast_Score=379, Evalue=1e-107,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): PUUR_ECOLI (P0A9U6)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   M38433
- PIR:   F64878
- RefSeq:   AP_001925.1
- RefSeq:   NP_415815.1
- ProteinModelPortal:   P0A9U6
- DIP:   DIP-48257N
- IntAct:   P0A9U6
- MINT:   MINT-1257910
- STRING:   P0A9U6
- EnsemblBacteria:   EBESCT00000003141
- EnsemblBacteria:   EBESCT00000014454
- GeneID:   945886
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1292
- KEGG:   eco:b1299
- EchoBASE:   EB2327
- EcoGene:   EG12431
- eggNOG:   COG1396
- GeneTree:   EBGT00050000009437
- HOGENOM:   HBG700107
- OMA:   SAHTPAN
- ProtClustDB:   PRK09943
- BioCyc:   EcoCyc:EG12431-MONOMER
- Genevestigator:   P0A9U6
- GO:   GO:0006350
- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710
- Gene3D:   G3DSA:2.60.120.10
- SMART:   SM00530

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3; SSF47413 Lambda_like_DNA; SSF51182 RmlC_like_cupin

EC number: NA

Molecular weight: Translated: 20092; Mature: 19961

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDKVSPAISTLQKLLKVYG
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
LSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGNPNRTLAMIFETYQPGTTT
CCHHHHHCCCCCCCCCEEEECCCHHHHHCCCCCEEEEEECCCCCCEEEEEEEECCCCCCH
GERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINTGIPHSFSNTSAGICRIISAH
HHHHHHHHHHHCEEECCEEEEEECCCEEEEECCCCEEECCCCCCCCCCCCCHHHEEEECC
TPTTF
CCCCC
>Mature Secondary Structure 
SDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDKVSPAISTLQKLLKVYG
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
LSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGNPNRTLAMIFETYQPGTTT
CCHHHHHCCCCCCCCCEEEECCCHHHHHCCCCCEEEEEECCCCCCEEEEEEEECCCCCCH
GERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINTGIPHSFSNTSAGICRIISAH
HHHHHHHHHHHCEEECCEEEEEECCCEEEEECCCCEEECCCCCCCCCCCCCHHHEEEECC
TPTTF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553