BacMap

Definition	Escherichia coli SMS-3-5 chromosome, complete genome.
Accession	NC_010498
Length	5,068,389

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The map label for this gene is ydfG [H]

Identifier: 170680775

GI number: 170680775

Start: 1615097

End: 1615843

Strand: Reverse

Name: ydfG [H]

Synonym: EcSMS35_1626

Alternate gene names: 170680775

Gene position: 1615843-1615097 (Counterclockwise)

Preceding gene: 170682596

Following gene: 170679935

Centisome position: 31.88

GC content: 51.0

Gene sequence:

>747_bases
ATGATCGTTTTAGTAACTGGAGCAACGGCAGGTTTTGGTGAATGCATTACTCGTCGTTTTATTCAACAAGGGCATAAAGT
TATCGCCACTGGCCGTCGCCAGGAGCGGTTGCAGGAGTTAAAAGACGAACTGGGAGATAATCTGTATATCGCCCAACTGG
ACGTTCGCAACCGCGCCGCTATTGAAGAGATGCTGGCATCGCTTCCTGCCGAGTGGTGCAATATTGATATCCTGGTAAAT
AATGCCGGTCTGGCGTTGGGCATGGAGCCTGCGCATAAAGCCAGCGTTGAAGACTGGGAAACGATGATTGATACCAACAA
CAAAGGCCTGGTATATATGACGCGCGCCGTCTTACCGGGTATGGTTGAACGTAATTATGGTCATATTATTAACATTGGCT
CAACGGCAGGCAGCTGGCCGTATGCCGGTGGTAACGTTTACGGTGCGACGAAAGCGTTTGTTCGTCAGTTTAGCCTGAAT
CTGCGTACGGACCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGCACCGAGTTTTCCAA
TGTCCGCTTTAAAGGCGATGACGGTAAAGCGGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCG
AAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTAT
GCCGGACTGAATGTCCACCGTCAGTAA

Upstream 100 bases:

>100_bases
GCTTACTGATAACGGAGTGCAATCTTGCAAACCAGTATTACCCGCTCTTAAGCATCACGTGCTATGTTATTGACACACAA
AAGCGTTGAGGAACAGTGAG

Downstream 100 bases:

>100_bases
TTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGC
AAGAACGAATGACCGTCGAA

Product: 3-hydroxy acid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDELGDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVN
NAGLALGMEPAHKASVEDWETMIDTNNKGLVYMTRAVLPGMVERNYGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWWVSTLPAHVNINTLEMMPVTQSY
AGLNVHRQ

Sequences:

>Translated_248_residues
MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDELGDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVN
NAGLALGMEPAHKASVEDWETMIDTNNKGLVYMTRAVLPGMVERNYGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWWVSTLPAHVNINTLEMMPVTQSY
AGLNVHRQ
>Mature_248_residues
MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDELGDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVN
NAGLALGMEPAHKASVEDWETMIDTNNKGLVYMTRAVLPGMVERNYGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWWVSTLPAHVNINTLEMMPVTQSY
AGLNVHRQ

Specific function: Catalyzes the NADP-dependent oxidation of L-allo- threonine and L-serine [H]

COG id: COG4221

COG function: function code R; Short-chain alcohol dehydrogenase of unknown specificity

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI109715829, Length=248, Percent_Identity=35.8870967741936, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI7657478, Length=263, Percent_Identity=30.4182509505703, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI20149619, Length=193, Percent_Identity=30.5699481865285, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI7705855, Length=204, Percent_Identity=29.4117647058824, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI150247226, Length=195, Percent_Identity=31.2820512820513, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI19923817, Length=232, Percent_Identity=27.5862068965517, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI22507405, Length=183, Percent_Identity=31.1475409836066, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI40548397, Length=184, Percent_Identity=30.9782608695652, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI214010158, Length=184, Percent_Identity=30.9782608695652, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI214010156, Length=184, Percent_Identity=30.9782608695652, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI22758144, Length=184, Percent_Identity=30.9782608695652, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI7706318, Length=216, Percent_Identity=31.4814814814815, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI32483357, Length=227, Percent_Identity=27.7533039647577, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI157739938, Length=187, Percent_Identity=26.2032085561497, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4557649, Length=182, Percent_Identity=30.2197802197802, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI119392083, Length=217, Percent_Identity=27.6497695852535, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI19743808, Length=183, Percent_Identity=27.8688524590164, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4503817, Length=183, Percent_Identity=27.3224043715847, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI5031737, Length=196, Percent_Identity=28.0612244897959, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4758504, Length=192, Percent_Identity=25.5208333333333, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI33667109, Length=190, Percent_Identity=27.8947368421053, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI50726952, Length=215, Percent_Identity=26.9767441860465, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI44680136, Length=184, Percent_Identity=30.4347826086957, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI44680133, Length=184, Percent_Identity=30.4347826086957, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI17738292, Length=184, Percent_Identity=30.4347826086957, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI4504503, Length=217, Percent_Identity=25.8064516129032, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI210032110, Length=189, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787820, Length=248, Percent_Identity=99.5967741935484, Blast_Score=513, Evalue=1e-147,
Organism=Escherichia coli, GI1787335, Length=196, Percent_Identity=33.6734693877551, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI87082100, Length=181, Percent_Identity=33.7016574585635, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786812, Length=176, Percent_Identity=30.6818181818182, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1790717, Length=179, Percent_Identity=26.2569832402235, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17562910, Length=214, Percent_Identity=30.8411214953271, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559104, Length=242, Percent_Identity=26.4462809917355, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71994600, Length=239, Percent_Identity=26.3598326359833, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17560332, Length=240, Percent_Identity=26.25, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17551412, Length=247, Percent_Identity=26.7206477732794, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538480, Length=190, Percent_Identity=26.8421052631579, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25152911, Length=221, Percent_Identity=30.316742081448, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994604, Length=238, Percent_Identity=25.6302521008403, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17544670, Length=243, Percent_Identity=25.9259259259259, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560150, Length=186, Percent_Identity=28.494623655914, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193204405, Length=191, Percent_Identity=29.3193717277487, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17565030, Length=191, Percent_Identity=31.413612565445, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17562908, Length=187, Percent_Identity=28.8770053475936, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562904, Length=217, Percent_Identity=29.0322580645161, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=190, Percent_Identity=27.8947368421053, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI212646214, Length=222, Percent_Identity=27.4774774774775, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17562990, Length=187, Percent_Identity=27.807486631016, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17560676, Length=232, Percent_Identity=24.1379310344828, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI193203115, Length=220, Percent_Identity=28.6363636363636, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=191, Percent_Identity=28.2722513089005, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193203117, Length=220, Percent_Identity=28.6363636363636, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17559552, Length=188, Percent_Identity=28.1914893617021, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=240, Percent_Identity=24.5833333333333, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17560176, Length=194, Percent_Identity=28.8659793814433, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=195, Percent_Identity=27.6923076923077, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17557668, Length=215, Percent_Identity=27.906976744186, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=152, Percent_Identity=29.6052631578947, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17531453, Length=187, Percent_Identity=27.807486631016, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=235, Percent_Identity=25.9574468085106, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=179, Percent_Identity=30.1675977653631, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17557780, Length=186, Percent_Identity=27.4193548387097, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=245, Percent_Identity=36.734693877551, Blast_Score=161, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6322227, Length=199, Percent_Identity=26.6331658291457, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6322067, Length=176, Percent_Identity=29.5454545454545, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322226, Length=190, Percent_Identity=23.6842105263158, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI116007236, Length=253, Percent_Identity=32.4110671936759, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI21358495, Length=223, Percent_Identity=29.5964125560538, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI23397609, Length=180, Percent_Identity=32.7777777777778, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24643142, Length=179, Percent_Identity=32.9608938547486, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24641388, Length=251, Percent_Identity=29.4820717131474, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI281364494, Length=253, Percent_Identity=27.2727272727273, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI116007238, Length=186, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24640442, Length=206, Percent_Identity=29.126213592233, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28571526, Length=233, Percent_Identity=28.3261802575107, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648693, Length=251, Percent_Identity=27.4900398406374, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21355889, Length=251, Percent_Identity=27.4900398406374, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24663911, Length=252, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24641232, Length=254, Percent_Identity=26.7716535433071, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571387, Length=252, Percent_Identity=25.7936507936508, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24584748, Length=181, Percent_Identity=29.8342541436464, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI161076933, Length=181, Percent_Identity=29.8342541436464, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21357041, Length=183, Percent_Identity=26.775956284153, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI116007234, Length=253, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI28574402, Length=186, Percent_Identity=31.1827956989247, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24584744, Length=186, Percent_Identity=31.1827956989247, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737361, Length=191, Percent_Identity=29.8429319371728, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24644339, Length=246, Percent_Identity=25.2032520325203, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24584752, Length=186, Percent_Identity=28.494623655914, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355631, Length=169, Percent_Identity=27.810650887574, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 27275; Mature: 27275

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDELGDNLYIAQLDVRNRAA
CEEEEECCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCHHH
IEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETMIDTNNKGLVYMTRAVLPG
HHHHHHHCCCCCCEEEEEEECCCEEECCCCCCCCCHHHHHHHHCCCCCCEEEEHHHHCCH
MVERNYGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLNLRTDLHGTAVRVTDIEPGLV
HHCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCC
GGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWWVSTLPAHVNINTLEMMPVTQSY
CCCCCCCEEEECCCCCCCHHHCCEEEECHHHHHHHHHHHHHCCCEEECEEEEEEECCCCC
AGLNVHRQ
CCCEEECC
>Mature Secondary Structure
MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDELGDNLYIAQLDVRNRAA
CEEEEECCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCHHH
IEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETMIDTNNKGLVYMTRAVLPG
HHHHHHHCCCCCCEEEEEEECCCEEECCCCCCCCCHHHHHHHHCCCCCCEEEEHHHHCCH
MVERNYGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLNLRTDLHGTAVRVTDIEPGLV
HHCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCC
GGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWWVSTLPAHVNINTLEMMPVTQSY
CCCCCCCEEEECCCCCCCHHHCCEEEECHHHHHHHHHHHHHCCCEEECEEEEEEECCCCC
AGLNVHRQ
CCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]