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The map label for this gene is glvA [H]

Identifier: 170680175

GI number: 170680175

Start: 4137430

End: 4138752

Strand: Reverse

Name: glvA [H]

Synonym: EcSMS35_4045

Alternate gene names: 170680175

Gene position: 4138752-4137430 (Counterclockwise)

Preceding gene: 170684221

Following gene: 170680795

Centisome position: 81.66

GC content: 53.21

Gene sequence:

>1323_bases
ATGACCAAATTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCA
GGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGATGGTGCGCGGCAGGAAGTGATTGCCGAAGCCTGTAAAG
TCATCCTTAAAGAAAAAGCGCCGGACATTGCGTTTAGTTACACCAACGATCCTGAAGTGGCATTCAGCGACGTTGATTTT
GTCATGGCGCACATCCGCGTCGGCAAATACCCGATGCGCGAACTGGATGAAAAAATCCCGCTGCGCCACGGCGTTGTTGG
TCAGGAAACATGCGGACCCGGCGGAATAGCGTACGGCATGCGTTCCATTGGCGGCGTCCTGGAACTGGTGGATTATATGG
AAAAATATTCACCAAATGCCTGGATGCTCAACTACTCCAACCCGGCAGCCATTGTCGCAGAAGCCACGCGTCGTCTGCGC
CCGAATGCGAAAATCCTCAACATCTGTGACATGCCAATCGGTATTGAAAGCCGGATGGCGCAAATTGTTGGGCTGCAAGA
TCGCAAACAGATGCGCGTGCGCTACTACGGCCTGAACCACTTTGGCTGGTGGACATCAATTGAAGATTTGCAGGGCAACG
ACCTGATGCCCCAGCTGCGGCAATATGTCTCTAAGCACGGTTATGTTCCACCGCAGCAAGATACACATACTGAAGCGAGC
TGGAACGACACCTATGCAAAAGCGCGGGATGTCCAGGCACTGGACCCGGATACATTGCCGAACACCTATCTGAAATATTA
TCTCTTCCCGGATTATGTCGTTCAGCATTCCAACCCTGAACATACCCGCGCGAATGAGGTGATGGAGCATCGCGAGAAAC
AGGTTTTCGATGCTTGCCGCGCCATTACGGCGGCAGGAAATTCAGCAGCGGGCAAGCTGGAAATTGACGAACATGCGTCA
TACATCGTCGATCTGGCGGCGGCAATTGCCTTCAACACTCAGGAGCGGATGTTGCTGATTGTGCCTAACAACGGGGCAAT
TCATAACTTTGATGATGAAGCGATGGTCGAGATCCCGTGTCTGGTCGGGCACAACGGACCAGAACCACTGGTGGTCGGCG
AGATCCCGCAGTTTCAGAAAGGGTTAATGAGTCAGCAAGTGGCGGTGGAAAAACTGGTCGTGGACGCCTGGGAGCAGCGT
TCATATCAGCACCTGTGGCAGGCGATTACGTTGTCGAAAACGGTACCGAGCGCCGCGGTCGCCAAAGCTATTCTGGATGA
GTTGCTGGAGGCCAACAAAGCGTACTGGCCAGAGTTACGTTAA

Upstream 100 bases:

>100_bases
TGATCATTGGTTTGCATGTATCCCAGCTGCGTGAACAGCTCGATAGCTTAATTAATTCTCATCAATCAGCAGAAAATGTT
GCCATTACGGAGGCAGTATA

Downstream 100 bases:

>100_bases
TTGTGTGGACTGGCATCCGTTGGGTGCTGGTCCGTATTCTTCAGCACAGCTGACGGCGCATAACCAAATTTATTTTTAAA
GGCTTTGCTGAAATGAAAGG

Product: maltose-6'-phosphate glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGARQEVIAEACKVILKEKAPDIAFSYTNDPEVAFSDVDF
VMAHIRVGKYPMRELDEKIPLRHGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQLRQYVSKHGYVPPQQDTHTEAS
WNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQHSNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHAS
YIVDLAAAIAFNTQERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGEIPQFQKGLMSQQVAVEKLVVDAWEQR
SYQHLWQAITLSKTVPSAAVAKAILDELLEANKAYWPELR

Sequences:

>Translated_440_residues
MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGARQEVIAEACKVILKEKAPDIAFSYTNDPEVAFSDVDF
VMAHIRVGKYPMRELDEKIPLRHGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQLRQYVSKHGYVPPQQDTHTEAS
WNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQHSNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHAS
YIVDLAAAIAFNTQERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGEIPQFQKGLMSQQVAVEKLVVDAWEQR
SYQHLWQAITLSKTVPSAAVAKAILDELLEANKAYWPELR
>Mature_439_residues
TKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGARQEVIAEACKVILKEKAPDIAFSYTNDPEVAFSDVDFV
MAHIRVGKYPMRELDEKIPLRHGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLRP
NAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQLRQYVSKHGYVPPQQDTHTEASW
NDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQHSNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASY
IVDLAAAIAFNTQERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGEIPQFQKGLMSQQVAVEKLVVDAWEQRS
YQHLWQAITLSKTVPSAAVAKAILDELLEANKAYWPELR

Specific function: Is involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). Hydrolyzes a wide variety of 6-phospho-alpha-D- glucosides including maltose-6'-phosphate, isomaltose-6'- phosphate, mal

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=451, Percent_Identity=27.0509977827051, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1790560, Length=457, Percent_Identity=21.6630196936543, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.122 [H]

Molecular weight: Translated: 49341; Mature: 49210

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGARQEVIAEACKVILKEKA
CCEEEEEEECCCCCCCHHHEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCC
PDIAFSYTNDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLRHGVVGQETCGPGGIAYGM
CCEEEEECCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHCCHHCCCCCCCCCCCCHHHHHH
RSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLRPNAKILNICDMPIGIESRMA
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
QIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQLRQYVSKHGYVPPQQDTHTEAS
HHHCCCCHHCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
WNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQHSNPEHTRANEVMEHREKQVFDACR
CCHHHHHHCCCCCCCCCCCCCHHEEEEECCHHHEECCCCCHHHHHHHHHHHHHHHHHHHH
AITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQERMLLIVPNNGAIHNFDDEAMVEIPC
HHHHCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEECCCCCCEEEEEE
LVGHNGPEPLVVGEIPQFQKGLMSQQVAVEKLVVDAWEQRSYQHLWQAITLSKTVPSAAV
EECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AKAILDELLEANKAYWPELR
HHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGARQEVIAEACKVILKEKA
CEEEEEEECCCCCCCHHHEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCC
PDIAFSYTNDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLRHGVVGQETCGPGGIAYGM
CCEEEEECCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHCCHHCCCCCCCCCCCCHHHHHH
RSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLRPNAKILNICDMPIGIESRMA
HHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
QIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQLRQYVSKHGYVPPQQDTHTEAS
HHHCCCCHHCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
WNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQHSNPEHTRANEVMEHREKQVFDACR
CCHHHHHHCCCCCCCCCCCCCHHEEEEECCHHHEECCCCCHHHHHHHHHHHHHHHHHHHH
AITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQERMLLIVPNNGAIHNFDDEAMVEIPC
HHHHCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEECCCCCCEEEEEE
LVGHNGPEPLVVGEIPQFQKGLMSQQVAVEKLVVDAWEQRSYQHLWQAITLSKTVPSAAV
EECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AKAILDELLEANKAYWPELR
HHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11473129; 11322729 [H]