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The map label for this gene is sotB [H]

Identifier: 170680058

GI number: 170680058

Start: 1630915

End: 1632105

Strand: Reverse

Name: sotB [H]

Synonym: EcSMS35_1642

Alternate gene names: 170680058

Gene position: 1632105-1630915 (Counterclockwise)

Preceding gene: 170680980

Following gene: 170683414

Centisome position: 32.2

GC content: 51.55

Gene sequence:

>1191_bases
ATGACAACAAACACTGTTTCCCGCAAAGTGGCGTGGCTACGGGTCGTTACGCTGGCAGTCGCCGCCTTCATCTTCAACAC
CACCGAATTTGTCCCAGTTGGCCTGCTCTCTGACATTGCGCAAAGTTTTCACATGCAAACCGCTCAGGTCGGCATTATGT
TGACCATTTACGCATGGGTAGTAGCGCTAATGTCATTGCCTTTTATGTTAATGACCAGCCAGGTTGAACGGCGCAAATTA
CTGATCTGCCTGTTTGTGGTGTTTATTGCCAGCCACGTACTGTCGTTTTTATCGTGGAGTTTTACCGTTCTGGTGATCAG
TCGCATTGGTGTGGCTTTTGCACATGCGATTTTCTGGTCGATTACGGCGTCTCTGGCGATCCGTATGGCTCCGGCCGGGA
AGCGAGCACAGGCATTGAGTTTAATTGCCACCGGTACTGCACTGGCGATGGTCTTAGGTTTACCTCTCGGGCGCATTGTG
GGGCAGTATTTCGGTTGGCGAATGACCTTCTTCGCGATTGGTATCGGGGCGCTTATCACCCTTTTGTGCCTGATTAAGTT
ACTTCCCTTACTGCCCAGTGAGCATTCCGGTTCGCTGAAAAGCCTCCCGCTATTATTCCGCCGCCCGGCATTGATGAGCA
TTTATTTGTTAACTGTGGTAGTTGTCACCGCCCATTACACGGCATACAGCTATATCGAGCCTTTTGTGCAAAACATTGCG
GGATTCAGCGCCAACTTTGCCACGGCATTACTGTTATTACTCGGTGGTGCGGGCATTATTGGCAGCGTGATTTTCGGTAA
ACTGGGTAATCAGTATGCGTCTACGCTGGTAAGTACGGCGATTGCGCTGTTGCTGGTGTGCCTGGCACTGCTGCTACCTG
CGGCGAACAGTGAAATACACCTCGGGGTGCTGAGTATTTTCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAG
GTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGGCGCTATTCTCCGGGATATTTAATATTGGAATCGG
CGCGGGTGCGTTGGTAGGTAATCAGGTCAGTCTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTT
TTGCCGCGTTAATATGGTCAATCATCATTTTCCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAG

Upstream 100 bases:

>100_bases
AACGGAAAACGCGATCCAGATCACAAATGCATTGTATTCACATCATTAACCGTTTTAAGATCATTTCATCACTTTTTCGC
AACTCACCCGATAATCTGTT

Downstream 100 bases:

>100_bases
TTGAAAGGCCCATTCGGGCCTTTTTTAGTGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACCCCCA
TACATACCAGCAGGAATCCC

Product: sugar efflux transporter

Products: arabinose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFHMQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKL
LICLFVVFIASHVLSFLSWSFTVLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLTVVVVTAHYTAYSYIEPFVQNIA
GFSANFATALLLLLGGAGIIGSVIFGKLGNQYASTLVSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQ
VKVLALAPDATDVAMALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLEEQTQ

Sequences:

>Translated_396_residues
MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFHMQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKL
LICLFVVFIASHVLSFLSWSFTVLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLTVVVVTAHYTAYSYIEPFVQNIA
GFSANFATALLLLLGGAGIIGSVIFGKLGNQYASTLVSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQ
VKVLALAPDATDVAMALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLEEQTQ
>Mature_395_residues
TTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFHMQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLL
ICLFVVFIASHVLSFLSWSFTVLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIVG
QYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLTVVVVTAHYTAYSYIEPFVQNIAG
FSANFATALLLLLGGAGIIGSVIFGKLGNQYASTLVSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQV
KVLALAPDATDVAMALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLEEQTQ

Specific function: Involved in the efflux of sugars. The physiological role may be the reduction of the intracellular concentration of toxic sugars or sugar metabolites [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. SotB (TC 2.A.1.2) family [H]

Homologues:

Organism=Escherichia coli, GI1787808, Length=396, Percent_Identity=99.7474747474748, Blast_Score=775, Evalue=0.0,
Organism=Escherichia coli, GI1787947, Length=326, Percent_Identity=30.0613496932515, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI145693198, Length=345, Percent_Identity=28.1159420289855, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1786595, Length=278, Percent_Identity=26.2589928057554, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42569; Mature: 42438

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFHMQTAQVGIMLTIYAWV
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFTVLVISRIGVAFAHAIFWS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIVGQYFGWRMTFFAIGIGALIT
HHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLTVVVVTAHYTAYSYIEPFVQNIA
HHHHHHHHHCCCCCCCCCHHHCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFSANFATALLLLLGGAGIIGSVIFGKLGNQYASTLVSTAIALLLVCLALLLPAANSEIH
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
LGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVAMALFSGIFNIGIGAGALVGNQVSLHW
HHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEE
SMSMIGYVGAVPAFAALIWSIIIFRRWPVTLEEQTQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: arabinose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: arabinose [Cytoplasm] + Proton [Periplasm] = arabinose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]