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The map label for this gene is ndhF

Identifier: 170077476

GI number: 170077476

Start: 881639

End: 883633

Strand: Direct

Name: ndhF

Synonym: SYNPCC7002_A0854

Alternate gene names: 170077476

Gene position: 881639-883633 (Clockwise)

Preceding gene: 170077468

Following gene: 170077477

Centisome position: 29.31

GC content: 52.58

Gene sequence:

>1995_bases
ATGGAACCGCTTTATCAATATGCATGGTTGATTCCGGTGCTGCCTCTGTTGGGGGCAATGGTTATCGGGATCGGCTTAAT
CTCCTTAAACAAATTCACGAACAAGCTACGGCAACTCAATGCGGTATTTGTTCTGTCGCTCATCGGCACTTCGATGGCCC
TCTCTTTTGGGCTACTCTGGAGTCAAATTCAGGGTCATGAAGCCTTTACCTACACCCTAGAATGGGCGGCGGCAGGCGAT
TTTCATCTGCAGATGGGCTATACCGTGGATCACCTGAGTGCCTTGATGTCGGTGATCGTAACCACGGTGGCCTTGCTGGT
GATGATCTATACCGATGGTTACATGGCCCACGATCCGGGTTATGTCCGTTTTTATGCTTATTTGAGCATTTTTAGCTCGT
CGATGTTGGGCTTGGTGTTTAGCCCAAACCTTGTGCAGGTTTACATTTTCTGGGAATTGGTGGGGATGTGTTCCTACCTC
CTGATTGGCTTCTGGTATGACCGCAAGGCGGCGGCCGATGCTTGCCAAAAGGCTTTTGTCACAAACCGTGTCGGCGATTT
TGGTCTCTTGCTGGGGATGCTCGGTCTCTATTGGGCCACGGGCAGTTTTGAGTTTGATCTGATGGGCGATCGCCTGATGG
ATCTCGTCTCGACGGGGCAAATCAGCTCTCTCCTGGCCATTGTCTTTGCTGTACTTGTGTTCCTTGGCCCTGTGGCTAAA
TCCGCCCAATTTCCGCTCCATGTGTGGCTCCCCGATGCCATGGAAGGGCCAACCCCCATTTCCGCTTTGATCCACGCGGC
GACCATGGTTGCTGCTGGAGTGTTCCTCGTGGCGCGGATGTATCCCGTGTTTGAGCCGATCCCAGAGGCGATGAATGTCA
TTGCCTGGACGGGGGCAACCACCGCTTTTCTAGGGGCGACCATTGCCTTAACCCAAAACGACATCAAAAAAGGTCTTGCC
TATTCCACCATGTCCCAGTTGGGCTACATGGTGATGGCCATGGGGATCGGTGGCTACACCGCTGGGTTGTTTCACCTGAT
GACCCATGCCTACTTCAAAGCGATGCTCTTCTTGGGTTCCGGTTCTGTCATCCATGGTATGGAAGAGGTGGTCGGCCATA
ATGCTGTGCTGGCCCAGGATATGCGGCTGATGGGTGGCCTGCGAAAATATATGCCCATCACTGCCACAACGTTTCTCATT
GGGACCCTCGCTATCTGTGGGATCCCGCCCTTTGCTGGTTTCTGGTCAAAGGACGAAATTTTGGGCCTGGCGTTTGAGGC
CAATCCTGTGCTCTGGTTCATTGGTTGGGCCACCGCTGGCATGACCGCTTTTTATATGTTCCGGATGTACTTCCTGACCT
TTGAAGGGGAGTTTCGCGGCACGGATCAGCAACTGCAAGAAAAGCTCCTGACGGCGGCAGGTCAAGCCCCCGAAGAAGGC
CACCATGGCTCTAAGCCCCACGAGTCTCCTTTGACGATGACTTTCCCGTTGATGGCTTTGGCAGTTCCGTCGGTATTGAT
CGGTTTGCTGGGGGTTCCCTGGGGTAATCGGTTTGAAGCTTTTGTCTTTTCTCCCAACGAAGCAGCAGAGGCCGCTGAGC
ATGGTTTTGAATTGACAGAATTTTTGATCATGGGTGGTAATTCTGTGGGGATCGCCCTCATTGGGATTACCATTGCCTCG
CTGATGTATCTCCAGCAGAGAATTGATCCGGCTCGTTTGGCCGAAAAATTCCCGGTGTTGTATCAGTTGTCTTTGAACAA
ATGGTACTTCGACGATATCTACAACAATGTTTTTGTGATGGGTACCCGGCGCTTAGCCCGACAAATCCTTGAGGTGGACT
ATCGCGTCGTCGATGGGGCCGTAAACCTCACGGGGATCGCCACTCTCCTGAGTGGTGAGGGCTTGAAATACATTGAAAAT
GGCCGCGTCCAGTTCTATGCGCTCATTGTGTTTGGGGCGGTGCTAGGCTTCGTCATTTTCTTTAGTGTGGCCTAG

Upstream 100 bases:

>100_bases
AAATGCCCTTGGTCAACTGTGCGCTGTCACCCAAAATTTGTTGGGAAATTTGACATCAATGGATGTGTAACTTAGAAAGC
AAGAATCCTAGGCATTCGTA

Downstream 100 bases:

>100_bases
CCGTCACTATATTCCGAAAGTTGAGAAGATAAAATTTCCCCTTGCCTGAGTGGTGAGGGGTTTTATTTTTCCTGATAAGA
TTTGCAGTGGCGTTGTTACA

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MEPLYQYAWLIPVLPLLGAMVIGIGLISLNKFTNKLRQLNAVFVLSLIGTSMALSFGLLWSQIQGHEAFTYTLEWAAAGD
FHLQMGYTVDHLSALMSVIVTTVALLVMIYTDGYMAHDPGYVRFYAYLSIFSSSMLGLVFSPNLVQVYIFWELVGMCSYL
LIGFWYDRKAAADACQKAFVTNRVGDFGLLLGMLGLYWATGSFEFDLMGDRLMDLVSTGQISSLLAIVFAVLVFLGPVAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARMYPVFEPIPEAMNVIAWTGATTAFLGATIALTQNDIKKGLA
YSTMSQLGYMVMAMGIGGYTAGLFHLMTHAYFKAMLFLGSGSVIHGMEEVVGHNAVLAQDMRLMGGLRKYMPITATTFLI
GTLAICGIPPFAGFWSKDEILGLAFEANPVLWFIGWATAGMTAFYMFRMYFLTFEGEFRGTDQQLQEKLLTAAGQAPEEG
HHGSKPHESPLTMTFPLMALAVPSVLIGLLGVPWGNRFEAFVFSPNEAAEAAEHGFELTEFLIMGGNSVGIALIGITIAS
LMYLQQRIDPARLAEKFPVLYQLSLNKWYFDDIYNNVFVMGTRRLARQILEVDYRVVDGAVNLTGIATLLSGEGLKYIEN
GRVQFYALIVFGAVLGFVIFFSVA

Sequences:

>Translated_664_residues
MEPLYQYAWLIPVLPLLGAMVIGIGLISLNKFTNKLRQLNAVFVLSLIGTSMALSFGLLWSQIQGHEAFTYTLEWAAAGD
FHLQMGYTVDHLSALMSVIVTTVALLVMIYTDGYMAHDPGYVRFYAYLSIFSSSMLGLVFSPNLVQVYIFWELVGMCSYL
LIGFWYDRKAAADACQKAFVTNRVGDFGLLLGMLGLYWATGSFEFDLMGDRLMDLVSTGQISSLLAIVFAVLVFLGPVAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARMYPVFEPIPEAMNVIAWTGATTAFLGATIALTQNDIKKGLA
YSTMSQLGYMVMAMGIGGYTAGLFHLMTHAYFKAMLFLGSGSVIHGMEEVVGHNAVLAQDMRLMGGLRKYMPITATTFLI
GTLAICGIPPFAGFWSKDEILGLAFEANPVLWFIGWATAGMTAFYMFRMYFLTFEGEFRGTDQQLQEKLLTAAGQAPEEG
HHGSKPHESPLTMTFPLMALAVPSVLIGLLGVPWGNRFEAFVFSPNEAAEAAEHGFELTEFLIMGGNSVGIALIGITIAS
LMYLQQRIDPARLAEKFPVLYQLSLNKWYFDDIYNNVFVMGTRRLARQILEVDYRVVDGAVNLTGIATLLSGEGLKYIEN
GRVQFYALIVFGAVLGFVIFFSVA
>Mature_664_residues
MEPLYQYAWLIPVLPLLGAMVIGIGLISLNKFTNKLRQLNAVFVLSLIGTSMALSFGLLWSQIQGHEAFTYTLEWAAAGD
FHLQMGYTVDHLSALMSVIVTTVALLVMIYTDGYMAHDPGYVRFYAYLSIFSSSMLGLVFSPNLVQVYIFWELVGMCSYL
LIGFWYDRKAAADACQKAFVTNRVGDFGLLLGMLGLYWATGSFEFDLMGDRLMDLVSTGQISSLLAIVFAVLVFLGPVAK
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARMYPVFEPIPEAMNVIAWTGATTAFLGATIALTQNDIKKGLA
YSTMSQLGYMVMAMGIGGYTAGLFHLMTHAYFKAMLFLGSGSVIHGMEEVVGHNAVLAQDMRLMGGLRKYMPITATTFLI
GTLAICGIPPFAGFWSKDEILGLAFEANPVLWFIGWATAGMTAFYMFRMYFLTFEGEFRGTDQQLQEKLLTAAGQAPEEG
HHGSKPHESPLTMTFPLMALAVPSVLIGLLGVPWGNRFEAFVFSPNEAAEAAEHGFELTEFLIMGGNSVGIALIGITIAS
LMYLQQRIDPARLAEKFPVLYQLSLNKWYFDDIYNNVFVMGTRRLARQILEVDYRVVDGAVNLTGIATLLSGEGLKYIEN
GRVQFYALIVFGAVLGFVIFFSVA

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family

Homologues:

Organism=Homo sapiens, GI251831117, Length=421, Percent_Identity=37.5296912114014, Blast_Score=261, Evalue=1e-69,
Organism=Escherichia coli, GI1788614, Length=590, Percent_Identity=37.2881355932203, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1788829, Length=441, Percent_Identity=31.5192743764172, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI1788827, Length=487, Percent_Identity=28.1314168377823, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1788831, Length=360, Percent_Identity=29.7222222222222, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI1788613, Length=448, Percent_Identity=26.1160714285714, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI2367154, Length=243, Percent_Identity=28.3950617283951, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI145693160, Length=304, Percent_Identity=26.9736842105263, Blast_Score=83, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NU5C_SYNP2 (P31971)

Other databases:

- EMBL:   M99378
- EMBL:   CP000951
- EMBL:   M86234
- RefSeq:   YP_001734114.1
- GeneID:   6057607
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A0854
- HOGENOM:   HBG643593
- OMA:   MIYTDGY
- ProtClustDB:   PRK07376
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393
- PRINTS:   PR01435
- TIGRFAMs:   TIGR01974

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N

EC number: 1.6.5.3

Molecular weight: Translated: 72879; Mature: 72879

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b3d5f34)-; HASH(0x1b3a0480)-; HASH(0x1a978d28)-; HASH(0x1b5d0cdc)-; HASH(0x1b71850c)-; HASH(0x1b877b94)-; HASH(0x1a2e2780)-; HASH(0x1ac99410)-; HASH(0x1ad4b63c)-; HASH(0x1b8de228)-; HASH(0x1bb85104)-; HASH(0x1a0263b8)-; HASH(0x1a84105c)-; HASH(0x1b0979d4)-; HASH(0x1b6e4714)-; HASH(0x1b59bb64)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPLYQYAWLIPVLPLLGAMVIGIGLISLNKFTNKLRQLNAVFVLSLIGTSMALSFGLLW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQIQGHEAFTYTLEWAAAGDFHLQMGYTVDHLSALMSVIVTTVALLVMIYTDGYMAHDPG
HHHCCCCEEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
YVRFYAYLSIFSSSMLGLVFSPNLVQVYIFWELVGMCSYLLIGFWYDRKAAADACQKAFV
HHHHHHHHHHHHHHHHHHEECCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TNRVGDFGLLLGMLGLYWATGSFEFDLMGDRLMDLVSTGQISSLLAIVFAVLVFLGPVAK
HHCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARMYPVFEPIPEAMNVIAWTGAT
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCH
TAFLGATIALTQNDIKKGLAYSTMSQLGYMVMAMGIGGYTAGLFHLMTHAYFKAMLFLGS
HHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
GSVIHGMEEVVGHNAVLAQDMRLMGGLRKYMPITATTFLIGTLAICGIPPFAGFWSKDEI
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCE
LGLAFEANPVLWFIGWATAGMTAFYMFRMYFLTFEGEFRGTDQQLQEKLLTAAGQAPEEG
EEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCCCC
HHGSKPHESPLTMTFPLMALAVPSVLIGLLGVPWGNRFEAFVFSPNEAAEAAEHGFELTE
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHCCCHHHH
FLIMGGNSVGIALIGITIASLMYLQQRIDPARLAEKFPVLYQLSLNKWYFDDIYNNVFVM
HHEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEEECCCEEHHHHHCCEEEE
GTRRLARQILEVDYRVVDGAVNLTGIATLLSGEGLKYIENGRVQFYALIVFGAVLGFVIF
EHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEHCCCCCHHHHHHHHHHHHHHHHH
FSVA
HHCH
>Mature Secondary Structure
MEPLYQYAWLIPVLPLLGAMVIGIGLISLNKFTNKLRQLNAVFVLSLIGTSMALSFGLLW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQIQGHEAFTYTLEWAAAGDFHLQMGYTVDHLSALMSVIVTTVALLVMIYTDGYMAHDPG
HHHCCCCEEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
YVRFYAYLSIFSSSMLGLVFSPNLVQVYIFWELVGMCSYLLIGFWYDRKAAADACQKAFV
HHHHHHHHHHHHHHHHHHEECCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TNRVGDFGLLLGMLGLYWATGSFEFDLMGDRLMDLVSTGQISSLLAIVFAVLVFLGPVAK
HHCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
SAQFPLHVWLPDAMEGPTPISALIHAATMVAAGVFLVARMYPVFEPIPEAMNVIAWTGAT
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCH
TAFLGATIALTQNDIKKGLAYSTMSQLGYMVMAMGIGGYTAGLFHLMTHAYFKAMLFLGS
HHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
GSVIHGMEEVVGHNAVLAQDMRLMGGLRKYMPITATTFLIGTLAICGIPPFAGFWSKDEI
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCE
LGLAFEANPVLWFIGWATAGMTAFYMFRMYFLTFEGEFRGTDQQLQEKLLTAAGQAPEEG
EEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCCCC
HHGSKPHESPLTMTFPLMALAVPSVLIGLLGVPWGNRFEAFVFSPNEAAEAAEHGFELTE
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHCCCHHHH
FLIMGGNSVGIALIGITIASLMYLQQRIDPARLAEKFPVLYQLSLNKWYFDDIYNNVFVM
HHEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEEECCCEEHHHHHCCEEEE
GTRRLARQILEVDYRVVDGAVNLTGIATLLSGEGLKYIENGRVQFYALIVFGAVLGFVIF
EHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEHCCCCCHHHHHHHHHHHHHHHHH
FSVA
HHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697