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The map label for this gene is ybiK [C]

Identifier: 170077446

GI number: 170077446

Start: 849520

End: 850446

Strand: Direct

Name: ybiK [C]

Synonym: SYNPCC7002_A0824

Alternate gene names: 170077446

Gene position: 849520-850446 (Clockwise)

Preceding gene: 170077445

Following gene: 170077447

Centisome position: 28.24

GC content: 54.91

Gene sequence:

>927_bases
GTGCAACCAAAACTCATTATTCACGGTGGGGCCGGCAGTTCCCTCAAGGGCAAAGGGGGCTTAATCGCCGTACGGGAGTC
GCTCCACAGGATTGTTCAAGAGGTTTATGACTTCCTAGAACAGGGCGGCAGTGCTGTGGAAGCCGTTATCCTAGGGGCAA
AATTATTGGAAAATGAGCCGCGTTTCAATGCAGGTACAGGGTCAGTGCTCCAGTCCGATGGTCAAATTCGCATGAGCGCT
TCCCTGATGGATGGTACGGCCCAAAAGTTTAGCGGCGTGATTAATGTGGGCCGCATTAAAAATCCCATCGAAGTGGCCCA
GGCGTTGCAAACAGAAGATGATCGGGTACTGTCCGATGTCGGTGCGGCAGAGATGGTGCGGGTAATGCAGCTCCCCATTC
ATGATCCTCTGACCGATTTACGCCTCGAAGAATGGCTGGAAGAACGTAAGGGAAATTTTCGCAGTAGTATGGCCGGGGTT
GTCGCGGAGGATGAAATGGCCCGGCGCGGCACCATTGGGGTGGTGGCTTTGGACCAAAATGGTCGGGTTGCAGCGGCCAC
ATCTACGGGCGGAAAAGGTTTAGAGCGGATTGGTCGGGTGAGTGATTCGGCGATGCCTGCGGGAAATTACGCGACTCAGG
TGGCTGGGGTTAGTTGCACGGGCATTGGGGAAGACATTATTGACGAATGCCTTGCGGCGAAGGTGGTTATTCGGGTCACG
GATGGTTTAAGTTTGCCAACCGCCATGGAAAAATCAATCCAGGAAGCAACGGCAAATAAACGGGATCTGGGGGCGATCGC
CTTGGATGCCGAGGGCAATATTGTCTGGGGAAAAACCAGCGAAGTGCTTTTGGCGGCCTACCACGACGGCACCGCTGTGG
GGGATACCCTGGAATGGCAACTGGAACCGGGGGTCGGTTGTCTCTAG

Upstream 100 bases:

>100_bases
GGGGAATTGGGCTGATACCGTAGGCAGGGCCGACATTTCTCGATAGAATTTCCTAGCGTTTGCTTTTAGTTAAAAATTCT
GGGGAATCAGTGGGATGACA

Downstream 100 bases:

>100_bases
GGAAGAGGGGAGGCCGATGGTGATGCGCACTCTTCTAAAACGTTTGGCGATCGCCCTGGGGACATTGCTTGCCATTAGTT
TTGTGATTTTCACGATTTTG

Product: L-asparagine amidohydrolase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MQPKLIIHGGAGSSLKGKGGLIAVRESLHRIVQEVYDFLEQGGSAVEAVILGAKLLENEPRFNAGTGSVLQSDGQIRMSA
SLMDGTAQKFSGVINVGRIKNPIEVAQALQTEDDRVLSDVGAAEMVRVMQLPIHDPLTDLRLEEWLEERKGNFRSSMAGV
VAEDEMARRGTIGVVALDQNGRVAAATSTGGKGLERIGRVSDSAMPAGNYATQVAGVSCTGIGEDIIDECLAAKVVIRVT
DGLSLPTAMEKSIQEATANKRDLGAIALDAEGNIVWGKTSEVLLAAYHDGTAVGDTLEWQLEPGVGCL

Sequences:

>Translated_308_residues
MQPKLIIHGGAGSSLKGKGGLIAVRESLHRIVQEVYDFLEQGGSAVEAVILGAKLLENEPRFNAGTGSVLQSDGQIRMSA
SLMDGTAQKFSGVINVGRIKNPIEVAQALQTEDDRVLSDVGAAEMVRVMQLPIHDPLTDLRLEEWLEERKGNFRSSMAGV
VAEDEMARRGTIGVVALDQNGRVAAATSTGGKGLERIGRVSDSAMPAGNYATQVAGVSCTGIGEDIIDECLAAKVVIRVT
DGLSLPTAMEKSIQEATANKRDLGAIALDAEGNIVWGKTSEVLLAAYHDGTAVGDTLEWQLEPGVGCL
>Mature_308_residues
MQPKLIIHGGAGSSLKGKGGLIAVRESLHRIVQEVYDFLEQGGSAVEAVILGAKLLENEPRFNAGTGSVLQSDGQIRMSA
SLMDGTAQKFSGVINVGRIKNPIEVAQALQTEDDRVLSDVGAAEMVRVMQLPIHDPLTDLRLEEWLEERKGNFRSSMAGV
VAEDEMARRGTIGVVALDQNGRVAAATSTGGKGLERIGRVSDSAMPAGNYATQVAGVSCTGIGEDIIDECLAAKVVIRVT
DGLSLPTAMEKSIQEATANKRDLGAIALDAEGNIVWGKTSEVLLAAYHDGTAVGDTLEWQLEPGVGCL

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Probably performs the final step in the degradation of the reserve polymer cyanophycin (depolymerizes the building block L-beta-Asp-Arg). Also has L- asparagin

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=311, Percent_Identity=34.4051446945338, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI145275200, Length=311, Percent_Identity=34.4051446945338, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1787050, Length=295, Percent_Identity=35.9322033898305, Blast_Score=119, Evalue=3e-28,
Organism=Drosophila melanogaster, GI18921183, Length=317, Percent_Identity=29.6529968454259, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357893, Length=327, Percent_Identity=26.2996941896024, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 32433; Mature: 32433

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPKLIIHGGAGSSLKGKGGLIAVRESLHRIVQEVYDFLEQGGSAVEAVILGAKLLENEP
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
RFNAGTGSVLQSDGQIRMSASLMDGTAQKFSGVINVGRIKNPIEVAQALQTEDDRVLSDV
CCCCCCCCCEECCCCEEEEEHHHHCHHHHHHCCEECCCCCCHHHHHHHHCCCCHHHHHHC
GAAEMVRVMQLPIHDPLTDLRLEEWLEERKGNFRSSMAGVVAEDEMARRGTIGVVALDQN
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHCCCEEEEEECCC
GRVAAATSTGGKGLERIGRVSDSAMPAGNYATQVAGVSCTGIGEDIIDECLAAKVVIRVT
CCEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHCCCCEECCCCHHHHHHHHHHEEEEEEE
DGLSLPTAMEKSIQEATANKRDLGAIALDAEGNIVWGKTSEVLLAAYHDGTAVGDTLEWQ
CCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCCCCEEEEEECCCCCCCCEEEEE
LEPGVGCL
ECCCCCCC
>Mature Secondary Structure
MQPKLIIHGGAGSSLKGKGGLIAVRESLHRIVQEVYDFLEQGGSAVEAVILGAKLLENEP
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
RFNAGTGSVLQSDGQIRMSASLMDGTAQKFSGVINVGRIKNPIEVAQALQTEDDRVLSDV
CCCCCCCCCEECCCCEEEEEHHHHCHHHHHHCCEECCCCCCHHHHHHHHCCCCHHHHHHC
GAAEMVRVMQLPIHDPLTDLRLEEWLEERKGNFRSSMAGVVAEDEMARRGTIGVVALDQN
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHCCCEEEEEECCC
GRVAAATSTGGKGLERIGRVSDSAMPAGNYATQVAGVSCTGIGEDIIDECLAAKVVIRVT
CCEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHCCCCEECCCCHHHHHHHHHHEEEEEEE
DGLSLPTAMEKSIQEATANKRDLGAIALDAEGNIVWGKTSEVLLAAYHDGTAVGDTLEWQ
CCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCCCCEEEEEECCCCCCCCEEEEE
LEPGVGCL
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]