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The map label for this gene is mnmG [H]

Identifier: 170026421

GI number: 170026421

Start: 4655533

End: 4657422

Strand: Direct

Name: mnmG [H]

Synonym: YPK_4217

Alternate gene names: 170026421

Gene position: 4655533-4657422 (Clockwise)

Preceding gene: 170026420

Following gene: 170026422

Centisome position: 99.28

GC content: 47.94

Gene sequence:

>1890_bases
ATGTTTTATCCAGATCAATTTGACGTCATCATCATCGGTGGTGGCCATGCAGGCACCGAAGCCGCCATGGCCGCGGCACG
CATGGGACGTCAGACCTTATTGCTGACGCACAATATCGATACTCTGGGGCAAATGTCTTGTAACCCAGCAATCGGCGGTA
TCGGCAAGGGGCACTTGGTCAAAGAAATTGATGCTCTGGGTGGCCTGATGGCGAAAGCAACAGACCTCGCGGGGATCCAG
TTTAGGATACTAAACGCCAGCAAAGGGCCTGCGGTAAGAGCAACCCGCGCTCAGGCTGACCGTGTTCTGTATCGTTTAGC
GGTACGAACGGCATTGGAGAATCAACCTAACTTGATGATCTTCCAGCAACCTGTTGAAGATCTCATTGTTGAAAATGATC
GTGTCGTTGGCGCAGTCACACAAATGGGTCTGAAATTCCGTGCCAAAGCGGTGGTGCTAACTGTTGGTACCTTCCTTGAT
GGCAAAATACATATTGGGCTAGAAAATTACAGTGGTGGCCGTGCCGGTGATCCCCCTTCGATCTCATTATCACAACGCTT
ACGTGAACTGCCTTTGCGAGTAAATCGCCTTAAAACGGGCACTCCACCACGTATCGATGCGCGAACTATCGACTTTAGCC
AATTAACACCTCAGTTGGGTGATACGCCAATTCCAGTATTCTCTTTCTTGGGAAATGCTGAACAACATCCTGAACAAATG
GCTTGTCATATCACCTACACCAATGAAAAAACACATGAAGTGATCCGCAATAACCTGGATCGCAGCCCAATGTATGCAGG
GATCATCGAAGGGATCGGCCCACGTTACTGCCCATCGATCGAAGATAAAGTCATGCGTTTTGCTGATCGTAATTCACATC
AGATCTTCCTCGAACCAGAGGGGCTAACCAGTAACGAGATCTATCCGAACGGCATTTCTACCAGTTTGCCTTTCGATGTA
CAGATGCAAATTGTCCGCTCAATGAAAGGGTTGGAAAATGCACGTATTATTCGTCCCGGCTATGCTATCGAATATGATTT
CTTTGATCCTCGCGATTTAAAACCGACTCTGGAAAGTAAATATATTCAAGGCTTGTTCTTTGCGGGACAAATCAATGGCA
CAACGGGCTACGAGGAAGCTGCGGCTCAAGGGCTGCTTGCAGGGCTTAATGCGGGCCGTTTTGCCAACGAGGAGGACGGA
TGGTCACCTCGGCGTGATGAAGCTTACCTTGGCGTGCTGGTGGACGATTTAAGCACTCTCGGCACTAAAGAACCGTACCG
TATGTTTACCTCCAGAGCTGAATACCGTTTGATGCTGCGTGAAGATAACGCTGATCTACGCCTGACTGAAACAGGCCGCA
AATTGGGATTAGTGGATGATATCCGGTGGGCACATTTCAGCCAAAAAGTTGAACAAATTGAAAAAGAGCGCCAACGGCTG
CGTGATATCTGGGTGCACCCGCATTCTGAGAATGTTTCAGAGATCAATGCGCTGCTGAAAGCACCTTTGTCCAAAGAAGC
CAACGGAGAAGAGCTACTGCGTCGGCCTGAAATAGACTATCGTTTGTTGACCTCTTTGACATCATTTGGCCCAGCGTTGA
CTGATCCACAATCGGCTGATCAAGTTGAAATTCAAGTCAAATATGAAGGGTATATTACTCGTCAGCAGGAAGAGATCGAA
AAACAACTACGCAACGAAAATACCTTATTGCCCGTTGATCTCGATTATCAGCAAGTCTCAGGTTTGTCGAATGAAGTCAT
TGCCAAATTGAATGACCATAAACCCAGTTCAATTGGTCAGGCATCCCGAATTTCAGGTATTACCCCCGCGGCAATATCTA
TCTTACTGGTTTGGCTGAAAAAACAAGGATTGTTGCGCCGCAGTGCGTGA

Upstream 100 bases:

>100_bases
AAAGGCGATCCTATGCCACTTGGTCGATCGTCTAAACTTGAGTAGAATCCCCCACCCCAGGGCAAACACAGAAATTCGCA
TAACGGCGAGGTGCAGTACC

Downstream 100 bases:

>100_bases
CGATTAAACGTTTGCCTGCACCCTGTATTTTACGGTCAGGCAAATACAGTAAACATATTCAATCCATTACCATGCTGGAT
TATCATTTTGCCTATTGACC

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MFYPDQFDVIIIGGGHAGTEAAMAAARMGRQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEIDALGGLMAKATDLAGIQ
FRILNASKGPAVRATRAQADRVLYRLAVRTALENQPNLMIFQQPVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLENYSGGRAGDPPSISLSQRLRELPLRVNRLKTGTPPRIDARTIDFSQLTPQLGDTPIPVFSFLGNAEQHPEQM
ACHITYTNEKTHEVIRNNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADRNSHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMKGLENARIIRPGYAIEYDFFDPRDLKPTLESKYIQGLFFAGQINGTTGYEEAAAQGLLAGLNAGRFANEEDG
WSPRRDEAYLGVLVDDLSTLGTKEPYRMFTSRAEYRLMLREDNADLRLTETGRKLGLVDDIRWAHFSQKVEQIEKERQRL
RDIWVHPHSENVSEINALLKAPLSKEANGEELLRRPEIDYRLLTSLTSFGPALTDPQSADQVEIQVKYEGYITRQQEEIE
KQLRNENTLLPVDLDYQQVSGLSNEVIAKLNDHKPSSIGQASRISGITPAAISILLVWLKKQGLLRRSA

Sequences:

>Translated_629_residues
MFYPDQFDVIIIGGGHAGTEAAMAAARMGRQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEIDALGGLMAKATDLAGIQ
FRILNASKGPAVRATRAQADRVLYRLAVRTALENQPNLMIFQQPVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLENYSGGRAGDPPSISLSQRLRELPLRVNRLKTGTPPRIDARTIDFSQLTPQLGDTPIPVFSFLGNAEQHPEQM
ACHITYTNEKTHEVIRNNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADRNSHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMKGLENARIIRPGYAIEYDFFDPRDLKPTLESKYIQGLFFAGQINGTTGYEEAAAQGLLAGLNAGRFANEEDG
WSPRRDEAYLGVLVDDLSTLGTKEPYRMFTSRAEYRLMLREDNADLRLTETGRKLGLVDDIRWAHFSQKVEQIEKERQRL
RDIWVHPHSENVSEINALLKAPLSKEANGEELLRRPEIDYRLLTSLTSFGPALTDPQSADQVEIQVKYEGYITRQQEEIE
KQLRNENTLLPVDLDYQQVSGLSNEVIAKLNDHKPSSIGQASRISGITPAAISILLVWLKKQGLLRRSA
>Mature_629_residues
MFYPDQFDVIIIGGGHAGTEAAMAAARMGRQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEIDALGGLMAKATDLAGIQ
FRILNASKGPAVRATRAQADRVLYRLAVRTALENQPNLMIFQQPVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLENYSGGRAGDPPSISLSQRLRELPLRVNRLKTGTPPRIDARTIDFSQLTPQLGDTPIPVFSFLGNAEQHPEQM
ACHITYTNEKTHEVIRNNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADRNSHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMKGLENARIIRPGYAIEYDFFDPRDLKPTLESKYIQGLFFAGQINGTTGYEEAAAQGLLAGLNAGRFANEEDG
WSPRRDEAYLGVLVDDLSTLGTKEPYRMFTSRAEYRLMLREDNADLRLTETGRKLGLVDDIRWAHFSQKVEQIEKERQRL
RDIWVHPHSENVSEINALLKAPLSKEANGEELLRRPEIDYRLLTSLTSFGPALTDPQSADQVEIQVKYEGYITRQQEEIE
KQLRNENTLLPVDLDYQQVSGLSNEVIAKLNDHKPSSIGQASRISGITPAAISILLVWLKKQGLLRRSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=637, Percent_Identity=45.6828885400314, Blast_Score=502, Evalue=1e-142,
Organism=Homo sapiens, GI19882217, Length=662, Percent_Identity=43.9577039274924, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI183227703, Length=677, Percent_Identity=42.9837518463811, Blast_Score=483, Evalue=1e-136,
Organism=Escherichia coli, GI2367273, Length=629, Percent_Identity=86.0095389507154, Blast_Score=1120, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=634, Percent_Identity=40.8517350157729, Blast_Score=472, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6321202, Length=620, Percent_Identity=45.6451612903226, Blast_Score=516, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24658174, Length=639, Percent_Identity=46.6353677621283, Blast_Score=527, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- InterPro:   IPR000048 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 70040; Mature: 70040

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYPDQFDVIIIGGGHAGTEAAMAAARMGRQTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCHHCCCCCCCCCCCCCCHHH
KEIDALGGLMAKATDLAGIQFRILNASKGPAVRATRAQADRVLYRLAVRTALENQPNLMI
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEE
FQQPVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLDGKIHIGLENYSGGRAGDPPS
ECCCHHHHHHCCCCEEEHHHHHCHHHCCEEEEEEEECEECCEEEEEEECCCCCCCCCCCC
ISLSQRLRELPLRVNRLKTGTPPRIDARTIDFSQLTPQLGDTPIPVFSFLGNAEQHPEQM
CCHHHHHHHCCHHHHHCCCCCCCCCCEEECCHHHHCCCCCCCCCHHHHHHCCCCCCCCCE
ACHITYTNEKTHEVIRNNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADRNSHQIFLEPE
EEEEEECCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
GLTSNEIYPNGISTSLPFDVQMQIVRSMKGLENARIIRPGYAIEYDFFDPRDLKPTLESK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCCCCCHHHHH
YIQGLFFAGQINGTTGYEEAAAQGLLAGLNAGRFANEEDGWSPRRDEAYLGVLVDDLSTL
HHCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHH
GTKEPYRMFTSRAEYRLMLREDNADLRLTETGRKLGLVDDIRWAHFSQKVEQIEKERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RDIWVHPHSENVSEINALLKAPLSKEANGEELLRRPEIDYRLLTSLTSFGPALTDPQSAD
HHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCC
QVEIQVKYEGYITRQQEEIEKQLRNENTLLPVDLDYQQVSGLSNEVIAKLNDHKPSSIGQ
EEEEEEEECCEEECCHHHHHHHHCCCCCEEEEECCHHHHCCCCHHHHHHCCCCCCCCCCC
ASRISGITPAAISILLVWLKKQGLLRRSA
HHHCCCCCHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MFYPDQFDVIIIGGGHAGTEAAMAAARMGRQTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCHHCCCCCCCCCCCCCCHHH
KEIDALGGLMAKATDLAGIQFRILNASKGPAVRATRAQADRVLYRLAVRTALENQPNLMI
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEE
FQQPVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLDGKIHIGLENYSGGRAGDPPS
ECCCHHHHHHCCCCEEEHHHHHCHHHCCEEEEEEEECEECCEEEEEEECCCCCCCCCCCC
ISLSQRLRELPLRVNRLKTGTPPRIDARTIDFSQLTPQLGDTPIPVFSFLGNAEQHPEQM
CCHHHHHHHCCHHHHHCCCCCCCCCCEEECCHHHHCCCCCCCCCHHHHHHCCCCCCCCCE
ACHITYTNEKTHEVIRNNLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADRNSHQIFLEPE
EEEEEECCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
GLTSNEIYPNGISTSLPFDVQMQIVRSMKGLENARIIRPGYAIEYDFFDPRDLKPTLESK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCCCCCHHHHH
YIQGLFFAGQINGTTGYEEAAAQGLLAGLNAGRFANEEDGWSPRRDEAYLGVLVDDLSTL
HHCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHH
GTKEPYRMFTSRAEYRLMLREDNADLRLTETGRKLGLVDDIRWAHFSQKVEQIEKERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RDIWVHPHSENVSEINALLKAPLSKEANGEELLRRPEIDYRLLTSLTSFGPALTDPQSAD
HHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCC
QVEIQVKYEGYITRQQEEIEKQLRNENTLLPVDLDYQQVSGLSNEVIAKLNDHKPSSIGQ
EEEEEEEECCEEECCHHHHHHHHCCCCCEEEEECCHHHHCCCCHHHHHHCCCCCCCCCCC
ASRISGITPAAISILLVWLKKQGLLRRSA
HHHCCCCCHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA