Click here to switch to the map view.

The map label for this gene is ilvG [H]

Identifier: 170026271

GI number: 170026271

Start: 4479136

End: 4480782

Strand: Reverse

Name: ilvG [H]

Synonym: YPK_4061

Alternate gene names: 170026271

Gene position: 4480782-4479136 (Counterclockwise)

Preceding gene: 170026273

Following gene: 170026270

Centisome position: 95.55

GC content: 53.19

Gene sequence:

>1647_bases
ATGAATGGTGCTCAGTGGGTGGTCCAAGCGTTGCGTGCGCAGGGCGTTGATACGGTATTTGGCTATCCGGGCGGGGCGAT
CATGCCTGTCTATGATGCGTTGTATGATGGTGGTGTTGAACATTTACTCTGCCGCCATGAGCAAGGTGCTGCCATTGCTG
CTATCGGTTATGCACGTGCAACCGGCAAAGTTGGGGTGTGTATCGCCACTTCTGGTCCCGGAGCGACTAACCTGATCACC
GGTCTGGCTGACGCTTTACTGGACTCTGTGCCGGTGATAGCTATCACCGGTCAGGTAGGGTCGGCCTTAATCGGTACCGA
TGCCTTTCAGGAGATCGATGTGCTGGGGCTATCACTGGCCTGCACCAAGCACAGTTTCTTGGTTGAGTCATTGGATGCGC
TGCCAGAGATCATGGCTGAAGCGTTTGCCATTGCCACTAGTGGCCGTCCTGGGCCGGTGTTAATCGATATTCCCAAAGAT
ATCCAGTTGGCGGTGGGGGAGTTAACGCCACACTTGAAGCCAGTTGAAGAGCATTCCGTTGATTCTGCCGCTGAGTTACA
ACACGCTTGGGATATGCTGGCGAACGCACAGAAACCGATGCTGTATGTTGGCGGTGGTGTCGGCATGGCGCAGGCGGTAC
CTGCCTTACGTGATTTTATCGCCATGACGGGGATCCCGGCGGTCGCCACCCTGAAAGGGCTGGGTGCACCAGATATCGCG
CACCCTTGTTATCTGGGTATGCTGGGTATGCACGGTACTAAAGCGGCGAATTTTGCCGTACAGGATTGTGATTTATTGGT
AGCGGTTGGCGCACGGTTTGATGACCGCGTAACCGGTAAGTTGAATACGTTCGCATCGAAAGCAAAAGTTATTCATATGG
ATATTGACCCGGCTGAGCTGGGCAAATTGCGTCAGGTTCATGTGGCATTGCAGGGCAATTTAAACGTGTTGTTGCCCGCC
TTACAACAGCCGTTAAATATTCAGCCGTGGCGTGATGAAGTGATGGCGCTGAAACAGCAACATCATTGGCGTTATGACCA
CCCTGGTCAGGCGATTTATGCGCCGTTATTACTGAAGCAGATCTCGGAGCGCAAAGCCCCTGAAACGGTAGTGACCACGG
ATGTGGGCCAGCACCAGATGTGGACGGCACAGCATATGAACTTTACCCGCCCGGAGAATTTCATCACTTCCAGCGGGTTG
GGGACCATGGGCTTCGGAGTGCCCGCTGCGGTAGGTGCTCAGATGGCCCGCCCTGATGATATGGTTATCTGTGTCTCTGG
CGATGGCTCCTTTATGATGAATGTGCAGGAGTTGGGCACGATAAAACGAAAACAGTTGCCGCTGAAAATCGTTTTATTGG
ATAACCAGCGGTTGGGGATGGTTCGGCAGTGGCAGCAATTGTTCTTCGATGGACGTTATAGCGAGACCAATCTTTCTGAT
AACCCCGATTTTATCACTCTGGCCAGCGCTTTTGATATCCCCGGCCAACGTATCACCCGTAAAGACCAAGTCGATGCCGC
TCTGGATGCGCTGTTTAATAGCGAAGGCCCTTATCTGCTCCAGGTTTCCATTGACGAACTCGAGAACGTTTGGCCGTTAG
TGCCGCCAGGCGCAGGTAATGAAACCATGCTGGAGAAAATCTCATGA

Upstream 100 bases:

>100_bases
GTCCGGGGGTTTTTTATTGGCACGCTAAAGGACGCGAGGAGCATAAAATGCACAGCAGAATAAAATCCTGTTCCTCTCGA
AAAAGGTCGGGGAAATAACT

Downstream 100 bases:

>100_bases
TACAACATCAACTCTCGATCCAAGCCCGCTTCCGCCCTGAAATGTTAGAGCGTGTCTTGCGCGTTGTGCGGCACCGTGGC
TTTCAGGTCTGTGCTATGAA

Product: acetolactate synthase 2 catalytic subunit

Products: NA

Alternate protein names: AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MNGAQWVVQALRAQGVDTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAIAAIGYARATGKVGVCIATSGPGATNLIT
GLADALLDSVPVIAITGQVGSALIGTDAFQEIDVLGLSLACTKHSFLVESLDALPEIMAEAFAIATSGRPGPVLIDIPKD
IQLAVGELTPHLKPVEEHSVDSAAELQHAWDMLANAQKPMLYVGGGVGMAQAVPALRDFIAMTGIPAVATLKGLGAPDIA
HPCYLGMLGMHGTKAANFAVQDCDLLVAVGARFDDRVTGKLNTFASKAKVIHMDIDPAELGKLRQVHVALQGNLNVLLPA
LQQPLNIQPWRDEVMALKQQHHWRYDHPGQAIYAPLLLKQISERKAPETVVTTDVGQHQMWTAQHMNFTRPENFITSSGL
GTMGFGVPAAVGAQMARPDDMVICVSGDGSFMMNVQELGTIKRKQLPLKIVLLDNQRLGMVRQWQQLFFDGRYSETNLSD
NPDFITLASAFDIPGQRITRKDQVDAALDALFNSEGPYLLQVSIDELENVWPLVPPGAGNETMLEKIS

Sequences:

>Translated_548_residues
MNGAQWVVQALRAQGVDTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAIAAIGYARATGKVGVCIATSGPGATNLIT
GLADALLDSVPVIAITGQVGSALIGTDAFQEIDVLGLSLACTKHSFLVESLDALPEIMAEAFAIATSGRPGPVLIDIPKD
IQLAVGELTPHLKPVEEHSVDSAAELQHAWDMLANAQKPMLYVGGGVGMAQAVPALRDFIAMTGIPAVATLKGLGAPDIA
HPCYLGMLGMHGTKAANFAVQDCDLLVAVGARFDDRVTGKLNTFASKAKVIHMDIDPAELGKLRQVHVALQGNLNVLLPA
LQQPLNIQPWRDEVMALKQQHHWRYDHPGQAIYAPLLLKQISERKAPETVVTTDVGQHQMWTAQHMNFTRPENFITSSGL
GTMGFGVPAAVGAQMARPDDMVICVSGDGSFMMNVQELGTIKRKQLPLKIVLLDNQRLGMVRQWQQLFFDGRYSETNLSD
NPDFITLASAFDIPGQRITRKDQVDAALDALFNSEGPYLLQVSIDELENVWPLVPPGAGNETMLEKIS
>Mature_548_residues
MNGAQWVVQALRAQGVDTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAIAAIGYARATGKVGVCIATSGPGATNLIT
GLADALLDSVPVIAITGQVGSALIGTDAFQEIDVLGLSLACTKHSFLVESLDALPEIMAEAFAIATSGRPGPVLIDIPKD
IQLAVGELTPHLKPVEEHSVDSAAELQHAWDMLANAQKPMLYVGGGVGMAQAVPALRDFIAMTGIPAVATLKGLGAPDIA
HPCYLGMLGMHGTKAANFAVQDCDLLVAVGARFDDRVTGKLNTFASKAKVIHMDIDPAELGKLRQVHVALQGNLNVLLPA
LQQPLNIQPWRDEVMALKQQHHWRYDHPGQAIYAPLLLKQISERKAPETVVTTDVGQHQMWTAQHMNFTRPENFITSSGL
GTMGFGVPAAVGAQMARPDDMVICVSGDGSFMMNVQELGTIKRKQLPLKIVLLDNQRLGMVRQWQQLFFDGRYSETNLSD
NPDFITLASAFDIPGQRITRKDQVDAALDALFNSEGPYLLQVSIDELENVWPLVPPGAGNETMLEKIS

Specific function: Catalyzes the first step in the biosynthesis of branched-chain amino acids [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=24.9116607773852, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI21361361, Length=496, Percent_Identity=24.7983870967742, Blast_Score=136, Evalue=5e-32,
Organism=Escherichia coli, GI1790104, Length=554, Percent_Identity=45.4873646209386, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=41.1660777385159, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1786717, Length=559, Percent_Identity=29.5169946332737, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1787096, Length=549, Percent_Identity=28.9617486338798, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1788716, Length=565, Percent_Identity=27.0796460176991, Blast_Score=159, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17531299, Length=559, Percent_Identity=27.5491949910555, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=559, Percent_Identity=27.5491949910555, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=504, Percent_Identity=23.4126984126984, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323755, Length=575, Percent_Identity=42.6086956521739, Blast_Score=452, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320588, Length=574, Percent_Identity=21.7770034843206, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320816, Length=494, Percent_Identity=22.6720647773279, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19922626, Length=563, Percent_Identity=25.9325044404973, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 58795; Mature: 58795

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGAQWVVQALRAQGVDTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAIAAIGYARA
CCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEEEC
TGKVGVCIATSGPGATNLITGLADALLDSVPVIAITGQVGSALIGTDAFQEIDVLGLSLA
CCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCHHHHHHHHHEEEEE
CTKHSFLVESLDALPEIMAEAFAIATSGRPGPVLIDIPKDIQLAVGELTPHLKPVEEHSV
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCHHHHHHHCCCCCCCCHHCCC
DSAAELQHAWDMLANAQKPMLYVGGGVGMAQAVPALRDFIAMTGIPAVATLKGLGAPDIA
CHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
HPCYLGMLGMHGTKAANFAVQDCDLLVAVGARFDDRVTGKLNTFASKAKVIHMDIDPAEL
CHHHHHHHCCCCCCCCCEEECCCCEEEEECCCCCCCCCCCHHHHHCCCEEEEEECCHHHH
GKLRQVHVALQGNLNVLLPALQQPLNIQPWRDEVMALKQQHHWRYDHPGQAIYAPLLLKQ
CCEEEEEEEEECCCEEEEHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ISERKAPETVVTTDVGQHQMWTAQHMNFTRPENFITSSGLGTMGFGVPAAVGAQMARPDD
HHHCCCCCEEEEECCCCCCEEHHHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHCCCCC
MVICVSGDGSFMMNVQELGTIKRKQLPLKIVLLDNQRLGMVRQWQQLFFDGRYSETNLSD
EEEEEECCCCEEEEHHHHCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCC
NPDFITLASAFDIPGQRITRKDQVDAALDALFNSEGPYLLQVSIDELENVWPLVPPGAGN
CCCEEEEEEECCCCCHHCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCC
ETMLEKIS
HHHHHHCC
>Mature Secondary Structure
MNGAQWVVQALRAQGVDTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAIAAIGYARA
CCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEEEC
TGKVGVCIATSGPGATNLITGLADALLDSVPVIAITGQVGSALIGTDAFQEIDVLGLSLA
CCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCHHHHHHHHHEEEEE
CTKHSFLVESLDALPEIMAEAFAIATSGRPGPVLIDIPKDIQLAVGELTPHLKPVEEHSV
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCHHHHHHHCCCCCCCCHHCCC
DSAAELQHAWDMLANAQKPMLYVGGGVGMAQAVPALRDFIAMTGIPAVATLKGLGAPDIA
CHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
HPCYLGMLGMHGTKAANFAVQDCDLLVAVGARFDDRVTGKLNTFASKAKVIHMDIDPAEL
CHHHHHHHCCCCCCCCCEEECCCCEEEEECCCCCCCCCCCHHHHHCCCEEEEEECCHHHH
GKLRQVHVALQGNLNVLLPALQQPLNIQPWRDEVMALKQQHHWRYDHPGQAIYAPLLLKQ
CCEEEEEEEEECCCEEEEHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
ISERKAPETVVTTDVGQHQMWTAQHMNFTRPENFITSSGLGTMGFGVPAAVGAQMARPDD
HHHCCCCCEEEEECCCCCCEEHHHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHCCCCC
MVICVSGDGSFMMNVQELGTIKRKQLPLKIVLLDNQRLGMVRQWQQLFFDGRYSETNLSD
EEEEEECCCCEEEEHHHHCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCC
NPDFITLASAFDIPGQRITRKDQVDAALDALFNSEGPYLLQVSIDELENVWPLVPPGAGN
CCCEEEEEEECCCCCHHCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCCCCCC
ETMLEKIS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3550695; 1379743; 9278503; 1995430; 6154938; 7015336; 3897211 [H]