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The map label for this gene is wecC

Identifier: 170026240

GI number: 170026240

Start: 4444390

End: 4445652

Strand: Reverse

Name: wecC

Synonym: YPK_4030

Alternate gene names: 170026240

Gene position: 4445652-4444390 (Counterclockwise)

Preceding gene: 170026241

Following gene: 170026239

Centisome position: 94.8

GC content: 49.8

Gene sequence:

>1263_bases
ATGAGTTTTGAAACTATTTCTGTTATCGGTCTTGGGTATATTGGTCTGCCGACCGCGGCGGCTTTTGCTTCGCGTAAAAA
GAAAGTGATTGGCGTTGACGTCAATGCGCATGCGGTTGAAACCATTAATCGTGGTGCCATCCATATCGTGGAGCCAGATT
TAGACAAAGTGGTAAAGATTGCTGTTGAAGGCGGCTACCTGCAAGCGGTGACGAAACCACAGGCAGCGGATGCATTCCTT
ATTGCCGTACCAACACCGTTTAAAGGCGATCATGAGCCGGATATGATTTTTGTCGAATCAGCCGCTAAATCCATCGCACC
GGTGCTGAAAAAAGGCGATTTGGTCATTTTGGAATCTACCTCCCCGGTAGGTGCTACTGAGCAAATGGCGCAATGGCTGG
CTGAGGCTCGCCCTGATTTGAGTTTCCCACAGCAAGCGGGCGAAGCAGCCGATATCAATATTGCATACTGCCCTGAGCGC
GTGTTACCGGGCCAGGTTATGGTTGAACTGATCCAGAATGACCGGGTGATTGGTGGCATGACACCAAAATGTTCTGCCCG
CGCCAGTGCGTTATATAAAATTTTCTTGGAAGGTGAGTGTGTGGTGACTAACTCACGCACCGCTGAGATGTGCAAACTGA
CGGAAAACAGCTTCCGTGACGTTAACATTGCCTTTGCCAACGAGTTATCACTGATTTGTGATGAGCAAGGTATCAATGTG
TGGGAGCTGATTCGTCTGGCGAACCGCCATCCACGCGTGAATATCTTACAGCCGGGTCCAGGTGTAGGTGGTCACTGTAT
CGCCGTCGATCCATGGTTTATTGTTTCTCAGAATCCACAATTGGCCCGCCTGATCCACACGGCGCGTCTGGTAAACGACG
GCAAACCGTTGTGGGTGGTTGATCGCGTTAAAGCCGCTGTTGCTGATTGTCTGGCCGCCAGTGATAAACGCGCTTCTGAA
GTGAAAATTGCCTGCTTCGGTCTGGCTTTTAAACCCGATATTGATGACCTGCGTGAAAGCCCAGCAGTGGGTGTTGCCCG
CTTGATCGCGGAGTGGCATGTGGGTGAAACACTGGTTGTTGAACCCAATGTTGAGCAATTGCCAAAATCACTGATGGGTC
TTGTCACCTTGAAAGATACCGCCACTGCTTTACAACAGGCTGATGTCTTGGTCATGTTGGTGGACCACAAACAATTCAAG
GCCATCAAACCTGAAGATATTAAGCAACAGTGGATTGTTGATACCAAAGGAGTATGGCGTTGA

Upstream 100 bases:

>100_bases
AGACGGCTTATCATCAAATGACCCGGGCACACAATCCTTACGGTGACGGGTATGCCTGTCAACGCATCCTTAAAGCTTTA
AAGAATCATCAGGTGACGCT

Downstream 100 bases:

>100_bases
GGCGAATTCTCGTCACAGGCGGGGCCGGTTTTATCGGCTCTGCGGTGGTCAGGCATATTATTGATGGCACCTCAGATAGC
GTGGTGGTGGTCGATAAACT

Product: UDP-N-acetyl-D-mannosamine dehydrogenase

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: UDP-ManNAc 6-dehydrogenase

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSFETISVIGLGYIGLPTAAAFASRKKKVIGVDVNAHAVETINRGAIHIVEPDLDKVVKIAVEGGYLQAVTKPQAADAFL
IAVPTPFKGDHEPDMIFVESAAKSIAPVLKKGDLVILESTSPVGATEQMAQWLAEARPDLSFPQQAGEAADINIAYCPER
VLPGQVMVELIQNDRVIGGMTPKCSARASALYKIFLEGECVVTNSRTAEMCKLTENSFRDVNIAFANELSLICDEQGINV
WELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVSQNPQLARLIHTARLVNDGKPLWVVDRVKAAVADCLAASDKRASE
VKIACFGLAFKPDIDDLRESPAVGVARLIAEWHVGETLVVEPNVEQLPKSLMGLVTLKDTATALQQADVLVMLVDHKQFK
AIKPEDIKQQWIVDTKGVWR

Sequences:

>Translated_420_residues
MSFETISVIGLGYIGLPTAAAFASRKKKVIGVDVNAHAVETINRGAIHIVEPDLDKVVKIAVEGGYLQAVTKPQAADAFL
IAVPTPFKGDHEPDMIFVESAAKSIAPVLKKGDLVILESTSPVGATEQMAQWLAEARPDLSFPQQAGEAADINIAYCPER
VLPGQVMVELIQNDRVIGGMTPKCSARASALYKIFLEGECVVTNSRTAEMCKLTENSFRDVNIAFANELSLICDEQGINV
WELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVSQNPQLARLIHTARLVNDGKPLWVVDRVKAAVADCLAASDKRASE
VKIACFGLAFKPDIDDLRESPAVGVARLIAEWHVGETLVVEPNVEQLPKSLMGLVTLKDTATALQQADVLVMLVDHKQFK
AIKPEDIKQQWIVDTKGVWR
>Mature_419_residues
SFETISVIGLGYIGLPTAAAFASRKKKVIGVDVNAHAVETINRGAIHIVEPDLDKVVKIAVEGGYLQAVTKPQAADAFLI
AVPTPFKGDHEPDMIFVESAAKSIAPVLKKGDLVILESTSPVGATEQMAQWLAEARPDLSFPQQAGEAADINIAYCPERV
LPGQVMVELIQNDRVIGGMTPKCSARASALYKIFLEGECVVTNSRTAEMCKLTENSFRDVNIAFANELSLICDEQGINVW
ELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVSQNPQLARLIHTARLVNDGKPLWVVDRVKAAVADCLAASDKRASEV
KIACFGLAFKPDIDDLRESPAVGVARLIAEWHVGETLVVEPNVEQLPKSLMGLVTLKDTATALQQADVLVMLVDHKQFKA
IKPEDIKQQWIVDTKGVWR

Specific function: Catalyzes the four-electron oxidation of UDP-N-acetyl-D- mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N- acetylmannosaminuronic acid (UDP-ManNAcA)

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family

Homologues:

Organism=Homo sapiens, GI4507813, Length=451, Percent_Identity=26.1640798226164, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI296040438, Length=347, Percent_Identity=25.0720461095101, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI296040443, Length=439, Percent_Identity=23.4624145785877, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI48994968, Length=420, Percent_Identity=82.3809523809524, Blast_Score=720, Evalue=0.0,
Organism=Escherichia coli, GI1788340, Length=406, Percent_Identity=24.384236453202, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17560350, Length=404, Percent_Identity=29.9504950495049, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI17136908, Length=456, Percent_Identity=25.6578947368421, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WECC_YERPE (Q8ZAE4)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AG0470
- RefSeq:   NP_667703.1
- RefSeq:   NP_994473.1
- RefSeq:   YP_002348741.1
- ProteinModelPortal:   Q8ZAE4
- SMR:   Q8ZAE4
- GeneID:   1145312
- GeneID:   1176702
- GeneID:   2765333
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO3863
- KEGG:   ypk:y0365
- KEGG:   ypm:YP_3182
- HOGENOM:   HBG400967
- OMA:   LVDHRQF
- ProtClustDB:   PRK11064
- BioCyc:   YPES187410:Y0365-MONOMER
- BioCyc:   YPES214092:YPO3863-MONOMER
- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11374
- PIRSF:   PIRSF000124
- TIGRFAMs:   TIGR03026

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N; SSF48179 6DGDH_C_like; SSF52413 UDP-Glc/GDP-Man_DH_C

EC number: 1.1.1.- [C]

Molecular weight: Translated: 45639; Mature: 45508

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: ACT_SITE 266-266

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFETISVIGLGYIGLPTAAAFASRKKKVIGVDVNAHAVETINRGAIHIVEPDLDKVVKI
CCCEEEEEEEECCCCCHHHHHHHHCCCEEEEEECCCHHHHHCCCCEEEEECCCHHHEEEE
AVEGGYLQAVTKPQAADAFLIAVPTPFKGDHEPDMIFVESAAKSIAPVLKKGDLVILEST
EECCCEEEEECCCCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHCCCCEEEEECC
SPVGATEQMAQWLAEARPDLSFPQQAGEAADINIAYCPERVLPGQVMVELIQNDRVIGGM
CCCCHHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEECC
TPKCSARASALYKIFLEGECVVTNSRTAEMCKLTENSFRDVNIAFANELSLICDEQGINV
CCCCHHHHHEEEEEEECCEEEEECCCCHHHHHHCCCCCCEEEEEEECCEEEEECCCCCCH
WELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVSQNPQLARLIHTARLVNDGKPLWVV
HHHHHHHCCCCEEEEECCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHCCCCCEEEH
DRVKAAVADCLAASDKRASEVKIACFGLAFKPDIDDLRESPAVGVARLIAEWHVGETLVV
HHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCHHHHHCCCCHHHHHHHHHHCCCCEEEE
EPNVEQLPKSLMGLVTLKDTATALQQADVLVMLVDHKQFKAIKPEDIKQQWIVDTKGVWR
CCCHHHHHHHHHHEEEEHHHHHHHHHCCEEEEEECCCHHCCCCCHHHHHHHEECCCCCCC
>Mature Secondary Structure 
SFETISVIGLGYIGLPTAAAFASRKKKVIGVDVNAHAVETINRGAIHIVEPDLDKVVKI
CCEEEEEEEECCCCCHHHHHHHHCCCEEEEEECCCHHHHHCCCCEEEEECCCHHHEEEE
AVEGGYLQAVTKPQAADAFLIAVPTPFKGDHEPDMIFVESAAKSIAPVLKKGDLVILEST
EECCCEEEEECCCCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHCCCCEEEEECC
SPVGATEQMAQWLAEARPDLSFPQQAGEAADINIAYCPERVLPGQVMVELIQNDRVIGGM
CCCCHHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEECC
TPKCSARASALYKIFLEGECVVTNSRTAEMCKLTENSFRDVNIAFANELSLICDEQGINV
CCCCHHHHHEEEEEEECCEEEEECCCCHHHHHHCCCCCCEEEEEEECCEEEEECCCCCCH
WELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVSQNPQLARLIHTARLVNDGKPLWVV
HHHHHHHCCCCEEEEECCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHCCCCCEEEH
DRVKAAVADCLAASDKRASEVKIACFGLAFKPDIDDLRESPAVGVARLIAEWHVGETLVV
HHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCHHHHHCCCCHHHHHHHHHHCCCCEEEE
EPNVEQLPKSLMGLVTLKDTATALQQADVLVMLVDHKQFKAIKPEDIKQQWIVDTKGVWR
CCCHHHHHHHHHHEEEEHHHHHHHHHCCEEEEEECCCHHCCCCCHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430