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The map label for this gene is dapF

Identifier: 170026222

GI number: 170026222

Start: 4423412

End: 4424236

Strand: Reverse

Name: dapF

Synonym: YPK_4011

Alternate gene names: 170026222

Gene position: 4424236-4423412 (Counterclockwise)

Preceding gene: 170026223

Following gene: 170026221

Centisome position: 94.34

GC content: 53.21

Gene sequence:

>825_bases
ATGCAGTTCTCCAAAATGCACGGTCTTGGCAACGACTTTATGGTTGTTGATGCAGTGACTCAAAATGTTTACTTTTCGCC
GGAATTGATCCGCCGATTAGCAGACCGGCACACGGGTGTGGGCTTTGATCAGATGCTGGTAGTTGAGCCGCCCTACGACC
CCGAGCTGGATTTTCACTACCGTATTTTTAATGCCGATGGGAGCGAAGTCTCCCAGTGTGGTAATGGCGCACGCTGCTTT
GCCCGTTTTGTTCGTTTGAAAGGGCTGACCAATAAGCGCGAGATCAGCGTCAGTACGCAAACTGGCCGTATGATCCTAAG
CGTGACAGAAGATGAACAGGTTTGCGTCAATATGGGCGAGCCTGATTTTGAACCGCAAACTGTCCCTTTTCGCGCCGCCA
AAGCGGAGAAAACCTATATTTTACGCGCCGCAGAGCATACGGTGCTGTGTGGCGTGGTGTCGATGGGCAACCCCCACTGT
GTGATGCAGGTCGATGATGTTTCGGTTGCCAACGTGGCGTTGCTCGGTCCAGTATTGGAAAACCACGAGCGTTTCCCTGA
GCGGGCAAATATCGGCTTTATGCAGGTCGTCAGTCGCGACCACATTCGTTTACGGGTGTATGAGCGTGGGGCCGGTGAAA
CACAAGCCTGTGGCAGTGGTGCATGTGCTGCGGTGGCCGTCGGGGTGGTGCAAGACTTATTGAACGAAAATGTGCATGTC
GAATTGCCGGGCGGCAGTTTACACATCCGTTGGCAGGGGCCAGGCCATCCGTTGTATATGACCGGGCCTGCCACCCATGT
CTATGACGGATTTATTCATTTATGA

Upstream 100 bases:

>100_bases
CATCAGTACTGATGCCAGCCCTGTATCGACTTAATCTGTATCGACGTTAGCCAACACCGAATTTAGCCAACAATAAAAAG
AGTAGTCAGCGGAGTGTGAT

Downstream 100 bases:

>100_bases
CGAGTGTTGATACATGGCGAGCGTTAATATATGAAGGGCAGTGATACATGAAGGGCGTTGATATATGAAGAATTATGAGG
AGCAGGCGTTAGCGGGCATC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQFSKMHGLGNDFMVVDAVTQNVYFSPELIRRLADRHTGVGFDQMLVVEPPYDPELDFHYRIFNADGSEVSQCGNGARCF
ARFVRLKGLTNKREISVSTQTGRMILSVTEDEQVCVNMGEPDFEPQTVPFRAAKAEKTYILRAAEHTVLCGVVSMGNPHC
VMQVDDVSVANVALLGPVLENHERFPERANIGFMQVVSRDHIRLRVYERGAGETQACGSGACAAVAVGVVQDLLNENVHV
ELPGGSLHIRWQGPGHPLYMTGPATHVYDGFIHL

Sequences:

>Translated_274_residues
MQFSKMHGLGNDFMVVDAVTQNVYFSPELIRRLADRHTGVGFDQMLVVEPPYDPELDFHYRIFNADGSEVSQCGNGARCF
ARFVRLKGLTNKREISVSTQTGRMILSVTEDEQVCVNMGEPDFEPQTVPFRAAKAEKTYILRAAEHTVLCGVVSMGNPHC
VMQVDDVSVANVALLGPVLENHERFPERANIGFMQVVSRDHIRLRVYERGAGETQACGSGACAAVAVGVVQDLLNENVHV
ELPGGSLHIRWQGPGHPLYMTGPATHVYDGFIHL
>Mature_274_residues
MQFSKMHGLGNDFMVVDAVTQNVYFSPELIRRLADRHTGVGFDQMLVVEPPYDPELDFHYRIFNADGSEVSQCGNGARCF
ARFVRLKGLTNKREISVSTQTGRMILSVTEDEQVCVNMGEPDFEPQTVPFRAAKAEKTYILRAAEHTVLCGVVSMGNPHC
VMQVDDVSVANVALLGPVLENHERFPERANIGFMQVVSRDHIRLRVYERGAGETQACGSGACAAVAVGVVQDLLNENVHV
ELPGGSLHIRWQGPGHPLYMTGPATHVYDGFIHL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=85.036496350365, Blast_Score=491, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_YERP3 (A7FD74)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001399202.1
- ProteinModelPortal:   A7FD74
- SMR:   A7FD74
- STRING:   A7FD74
- GeneID:   5386842
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_0205
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   YPSE349747:YPSIP31758_0205-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30253; Mature: 30253

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 73-73 ACT_SITE 217-217

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSKMHGLGNDFMVVDAVTQNVYFSPELIRRLADRHTGVGFDQMLVVEPPYDPELDFHY
CCCHHHCCCCCCEEEEEEECCCEEECHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEE
RIFNADGSEVSQCGNGARCFARFVRLKGLTNKREISVSTQTGRMILSVTEDEQVCVNMGE
EEEECCCHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCEEEEECCC
PDFEPQTVPFRAAKAEKTYILRAAEHTVLCGVVSMGNPHCVMQVDDVSVANVALLGPVLE
CCCCCCCCCEEECCCCCEEEEEECCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHH
NHERFPERANIGFMQVVSRDHIRLRVYERGAGETQACGSGACAAVAVGVVQDLLNENVHV
HHHCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
ELPGGSLHIRWQGPGHPLYMTGPATHVYDGFIHL
EECCCEEEEEEECCCCCEEEECCCHHCCCCEEEC
>Mature Secondary Structure
MQFSKMHGLGNDFMVVDAVTQNVYFSPELIRRLADRHTGVGFDQMLVVEPPYDPELDFHY
CCCHHHCCCCCCEEEEEEECCCEEECHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEE
RIFNADGSEVSQCGNGARCFARFVRLKGLTNKREISVSTQTGRMILSVTEDEQVCVNMGE
EEEECCCHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCEEEEECCC
PDFEPQTVPFRAAKAEKTYILRAAEHTVLCGVVSMGNPHCVMQVDDVSVANVALLGPVLE
CCCCCCCCCEEECCCCCEEEEEECCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHH
NHERFPERANIGFMQVVSRDHIRLRVYERGAGETQACGSGACAAVAVGVVQDLLNENVHV
HHHCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
ELPGGSLHIRWQGPGHPLYMTGPATHVYDGFIHL
EECCCEEEEEEECCCCCEEEECCCHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA